{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:20:52Z","timestamp":1788909652316,"version":"build-2803163510"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Brazilian Council for Scientific Research"},{"name":"Carlos Chagas\u00a0Filho Foundation of Research Support of the State of Rio de Janeiro"},{"name":"Higher Education Coordination of Improvement"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.bios.2017.08.029","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T15:05:57Z","timestamp":1502982357000},"page":"115-121","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Development of an electrochemical immunosensor for the diagnostic testing of spotted fever using synthetic peptides"],"prefix":"10.1016","volume":"100","author":[{"given":"Isis C.","family":"Prado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f4nica E.T.A.","family":"Chino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia L.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 L.A.","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano G.","family":"Pinho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elba R.S.","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore G.","family":"De-Simone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2017.08.029_bib1","series-title":"Cellular and Molecular Immunology","author":"Abbas","year":"2007"},{"key":"10.1016\/j.bios.2017.08.029_bib2","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1128\/jcm.10.5.677-684.1979","article-title":"Indirect hemagglutination test for detection of antibody to Rickettsia rickettsii in sera from humans and common laboratory animals","volume":"10","author":"Anacker","year":"1979","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.bios.2017.08.029_bib3","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1186\/1471-2334-13-568","article-title":"Chagas disease-specific antigens: characterization of epitopes in CRA\/FRA by synthetic peptide mapping and evaluation by ELISA-peptide assay","volume":"13","author":"Bottino","year":"2013","journal-title":"BMC Infect. Dis."},{"issue":"9.4","key":"10.1016\/j.bios.2017.08.029_bib4","article-title":"B cell epitope mapping using synthetic peptides","volume":"60","author":"Carter","year":"2004","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.bios.2017.08.029_bib5","unstructured":"Casey, H.L., 1965. Adaptation of LBCF method to microtechnique. Standardized diagnostic complement fixation method and adaptation to the microtest. In: U.S. Public Health Service Monograph no. 74. U.S. Government Printing Office, Washington, D.C., pp. 31\u201334."},{"key":"10.1016\/j.bios.2017.08.029_bib6","series-title":"Din\u00e2mica das doen\u00e7as infecciosas e parasit\u00e1rias","author":"Coura","year":"2005"},{"issue":"4","key":"10.1016\/j.bios.2017.08.029_bib7","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.trstmh.2007.11.008","article-title":"Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of Schistosomiasis mansoni","volume":"102","author":"De Oliveira","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"4","key":"10.1016\/j.bios.2017.08.029_bib8","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/S0123-9392(10)70120-X","article-title":"Fiebre manchada de las monta\u00f1as rocosas: ni tan manchada ni tan monta\u00f1osa como pens\u00e1bamos","volume":"14","author":"D\u00edaz","year":"2010","journal-title":"Infectio"},{"issue":"8","key":"10.1016\/j.bios.2017.08.029_bib9","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1590\/S0074-02762005000800004","article-title":"Detection of Rickettsia rickettsii in the tick Amblyomma cajennense in a new Brazilian spotted fever-endemic area in the state of Minas Gerais","volume":"100","author":"Guedes","year":"2005","journal-title":"Mem Inst. Oswaldo Cruz"},{"key":"10.1016\/j.bios.2017.08.029_bib10","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1128\/jcm.12.2.144-150.1980","article-title":"Detection of rocky mountain spotted fever antibodies by a latex agglutination test","volume":"12","author":"Hechemy","year":"1980","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.bios.2017.08.029_bib11","doi-asserted-by":"crossref","first-page":"A23","DOI":"10.1016\/S0264-410X(02)00383-3","article-title":"Outer membrane protein A (OmpA): a new pathogen-associated molecular pattern that interacts with antigen presenting cells\u2014impact on vaccine strategies","volume":"20","author":"Jeannin","year":"2002","journal-title":"Vaccine"},{"key":"10.1016\/j.bios.2017.08.029_bib12","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.trac.2015.11.020","article-title":"Electrochemical immunosensors: critical survey of different architectures and transduction strategies","volume":"79","author":"Kokkinos","year":"2016","journal-title":"Trends Anal. Chem."},{"issue":"4","key":"10.1016\/j.bios.2017.08.029_bib13","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/0092-8674(90)90464-P","article-title":"Epitopes on protein antigens: misconceptions and realities","volume":"61","author":"Laver","year":"1990","journal-title":"Cell"},{"issue":"12","key":"10.1016\/j.bios.2017.08.029_bib14","doi-asserted-by":"crossref","first-page":"e115105","DOI":"10.1371\/journal.pone.0115105","article-title":"Clinical presentation, convalescence, and relapse of rocky mountain spotted fever in dogs experimentally infected via tick bite","volume":"9","author":"Levin","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.bios.2017.08.029_bib15","series-title":"Structural Biology: With Biochemical and Biophysical Foundations","author":"Luckey","year":"2008"},{"key":"10.1016\/j.bios.2017.08.029_bib16","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0956-5663(91)85016-P","article-title":"Highly-sensitive measurement of hydroquinone with an enzyme electrode","volume":"6","author":"Mizutani","year":"1991","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2017.08.029_bib17","series-title":"Rapid Detection of Infectious Agents","first-page":"21","article-title":"Use of synthetic peptides in microbial diagnostics","author":"Modrow","year":"2002"},{"key":"10.1016\/j.bios.2017.08.029_bib18","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1186\/1471-2164-13-510","article-title":"Is the C-terminal insertional signal in Gram-negative bacterial outer membrane proteins species-specific or not?","volume":"13","author":"Paramasivam","year":"2012","journal-title":"BMC Genom."},{"key":"10.1016\/j.bios.2017.08.029_bib19","series-title":"O Sistema Imune","author":"Parham","year":"2011"},{"key":"10.1016\/j.bios.2017.08.029_bib20","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1128\/CMR.18.4.719-756.2005","article-title":"Tick-borne rickettsioses around the world: emerging diseases challenging old concepts","volume":"18","author":"Parola","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.bios.2017.08.029_bib21","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick-borne rickettsioses around the world: a geographic approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.bios.2017.08.029_bib22","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.clindermatol.2005.11.007","article-title":"Tropical rickettsioses","volume":"24","author":"Parola","year":"2006","journal-title":"Clin. Dermatol."},{"key":"10.1016\/j.bios.2017.08.029_bib23","doi-asserted-by":"crossref","first-page":"5364","DOI":"10.1021\/ac900464z","article-title":"Comparative study of different cross-linking agents for the imobilization of functionalized carbon nanotubes within a chitosan film supported on a graphite \u2013 epoxy composite","volume":"81","author":"Pauliukaite","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2017.08.029_bib24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1006\/jmbi.2000.3671","article-title":"High-resolution structure of the OmpA membrane domain","volume":"298","author":"Pautsch","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bios.2017.08.029_bib25","series-title":"Typho exanthematico de S\u00e3o Paulo","author":"Piza","year":"1932"},{"issue":"1","key":"10.1016\/j.bios.2017.08.029_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000260078","article-title":"Relevant B cell epitopes in allergic disease","volume":"152","author":"Pom\u00e9s","year":"2010","journal-title":"Int. Arch. Allergy Immunol."},{"issue":"7","key":"10.1016\/j.bios.2017.08.029_bib27","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.resmic.2006.02.002","article-title":"Identification of two putative rickettsial adhesins by proteomic analysis","volume":"157","author":"Renesto","year":"2006","journal-title":"Res. Microbiol."},{"issue":"1","key":"10.1016\/j.bios.2017.08.029_bib28","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1574-695X.2011.00899.x","article-title":"Widespread use of real-time PCR for rickettsial diagnosis","volume":"64","author":"Renvois\u00e9","year":"2012","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.bios.2017.08.029_bib29","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.bios.2016.05.004","article-title":"Cratylia mollis lectin nanoelectrode for differential diagnostic of prostate cancer and benign prostatic hyperplasia based on label-free detection","volume":"85","author":"Silva","year":"2016","journal-title":"Biosens. Bioelectron."},{"issue":"2","key":"10.1016\/j.bios.2017.08.029_bib30","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1006\/bbrc.2002.6657","article-title":"The function of OmpA in Escherichia coli","volume":"292","author":"Wang","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bios.2017.08.029_bib31","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1128\/iai.52.1.120-127.1986","article-title":"Humoral immune response to rocky mountain spotted fever in experimentally Iifected guinea pigs: immunoprecipitation of lactoperoxidase 125I-labeled proteins and detection of soluble antigens of Rickettsia rickettsii","volume":"52","author":"Williams","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bios.2017.08.029_bib32","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.bios.2004.01.013","article-title":"Hydroquinone Diphosphate: an alkaline phosphate substrate that does not produce electrode fouling in electrochemical imunoassays","volume":"20","author":"Wilson","year":"2004","journal-title":"Biosens. Bioelectron."},{"issue":"4","key":"10.1016\/j.bios.2017.08.029_bib33","doi-asserted-by":"crossref","first-page":"e62249","DOI":"10.1371\/journal.pone.0062249","article-title":"Conformational B-cell epitope prediction on antigen protein structures: a review of current algorithms and comparison with common binding site prediction methods","volume":"8","author":"Yao","year":"2013","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.bios.2017.08.029_bib34","doi-asserted-by":"crossref","first-page":"903","DOI":"10.4269\/ajtmh.2008.79.903","article-title":"Rickettsia felis outer membrane protein a: a potential tool for diagnosis of patients with flea-borne spotted fever","volume":"79","author":"Zavala-Castro","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"24","key":"10.1016\/j.bios.2017.08.029_bib35","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.bios.2008.03.017","article-title":"Highly sensitive electrochemical detection of immunospecies based on combination of Fc label and PPD film\/gold nanoparticle amplification","volume":"15","author":"Zhang","year":"2008","journal-title":"Biosens. Bioelectron."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566317305651?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566317305651?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:41:24Z","timestamp":1760661684000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566317305651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":35,"alternative-id":["S0956566317305651"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2017.08.029","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of an electrochemical immunosensor for the diagnostic testing of spotted fever using synthetic peptides","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2017.08.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}