{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T15:45:45Z","timestamp":1792856745473,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.bios.2020.112774","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T02:22:08Z","timestamp":1604110928000},"page":"112774","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"special_numbering":"C","title":["Glial-fibrillary-acidic-protein (GFAP) biomarker detection in serum-matrix: Functionalization strategies and detection by an ultra-high-frequency surface-acoustic-wave (UHF-SAW) lab-on-chip."],"prefix":"10.1016","volume":"172","author":[{"given":"M.","family":"Agostini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Amato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.L.","family":"Vieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Greco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Tonazzini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Baroncelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Caleo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Vannini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Santi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Signore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cecchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2020.112774_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-33158-8","article-title":"Serum GFAP as a biomarker for disease severity in multiple sclerosis","volume":"8","author":"Abdelhak","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bios.2020.112774_bib2","doi-asserted-by":"crossref","first-page":"70901","DOI":"10.1109\/ACCESS.2019.2919000","article-title":"Full-SAW microfluidics-based lab-on-a-chip for biosensing","volume":"7","author":"Agostini","year":"2019","journal-title":"IEEE Access"},{"key":"10.1016\/j.bios.2020.112774_bib3","doi-asserted-by":"crossref","DOI":"10.1063\/1.5070136","article-title":"Polydimethylsiloxane (PDMS) irreversible bonding to untreated plastics and metals for microfluidics applications","volume":"7","author":"Agostini","year":"2019","journal-title":"Apl. Mater."},{"key":"10.1016\/j.bios.2020.112774_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.snb.2017.07.014","article-title":"A Rayleigh surface acoustic wave (R-SAW) resonator biosensor based on positive and negative reflectors with sub-nanomolar limit of detection","volume":"254","author":"Agostini","year":"2018","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2020.112774_bib5","doi-asserted-by":"crossref","first-page":"6770","DOI":"10.1021\/la401109r","article-title":"Effects of the electrode size and modification protocol on a label-free electrochemical biosensor","volume":"29","author":"Arya","year":"2013","journal-title":"Langmuir"},{"key":"10.1016\/j.bios.2020.112774_bib6","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1007\/s00415-010-5863-2","article-title":"Glial fibrillary acidic protein: a potential biomarker for progression in multiple sclerosis","volume":"258","author":"Axelsson","year":"2011","journal-title":"J. Neurol."},{"key":"10.1016\/j.bios.2020.112774_bib7","doi-asserted-by":"crossref","first-page":"104103","DOI":"10.1063\/1.2889951","article-title":"Acoustic-counterflow microfluidics by surface acoustic waves","volume":"92","author":"Cecchini","year":"2008","journal-title":"Appl. Phys. Lett."},{"key":"10.1016\/j.bios.2020.112774_bib8","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.bios.2014.12.058","article-title":"A surface-acoustic-wave-based cantilever bio-sensor","volume":"68","author":"De Simoni","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2020.112774_bib9","doi-asserted-by":"crossref","first-page":"2722","DOI":"10.1039\/C5LC00265F","article-title":"Recent advances in microfluidic actuation and micro-object manipulation via surface acoustic waves","volume":"15","author":"Destgeer","year":"2015","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2020.112774_bib10","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1016\/j.snb.2013.07.099","article-title":"Reusable gold nanoparticle enhanced QCM immunosensor for detecting C-reactive protein","volume":"188","author":"Ding","year":"2013","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2020.112774_bib11","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1039\/b906819h","article-title":"Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices","volume":"9","author":"Franke","year":"2009","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2020.112774_bib12","first-page":"1","article-title":"The association of very early serum levels of S100B, GFAP, UCH-L1, and SBDP with outcome in ProTECT III","author":"Frankel","year":"2019","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.bios.2020.112774_bib13","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1103\/RevModPhys.83.647","article-title":"Microscale acoustofluidics: microfluidics driven via acoustics and ultrasonics","volume":"83","author":"Friend","year":"2011","journal-title":"Rev. Mod. Phys."},{"key":"10.1016\/j.bios.2020.112774_bib14","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.snb.2017.04.186","article-title":"Embryo development in dynamic microfluidic systems","volume":"250","author":"Greco","year":"2017","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2020.112774_bib15","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.3390\/s17112452","article-title":"Surface acoustic wave (SAW)-Enhanced chemical functionalization of gold films","volume":"17","author":"Greco","year":"2017","journal-title":"Sensors"},{"key":"10.1016\/j.bios.2020.112774_bib16","doi-asserted-by":"crossref","first-page":"7450","DOI":"10.1021\/acs.analchem.8b00972","article-title":"Surface-acoustic-wave (SAW)-Driven device for dynamic cell cultures","volume":"90","author":"Greco","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2020.112774_bib17","first-page":"5","article-title":"The costs of traumatic brain injury: a literature review","author":"Humphreys","year":"2013","journal-title":"Clin. Outcomes Res."},{"key":"10.1016\/j.bios.2020.112774_bib18","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1093\/brain\/awm263","article-title":"Serum GFAP is a diagnostic marker for glioblastoma multiforme","volume":"130","author":"Jung","year":"2007","journal-title":"Brain"},{"key":"10.1016\/j.bios.2020.112774_bib19","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.apgeochem.2006.09.010","article-title":"Sorption isotherms: a review on physical bases, modeling and measurement","volume":"22","author":"Limousin","year":"2007","journal-title":"Appl. Geochem."},{"key":"10.1016\/j.bios.2020.112774_bib20","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1039\/C4PY01356E","article-title":"Antibiofouling polymer interfaces: poly(ethylene glycol) and other promising candidates","volume":"6","author":"Lowe","year":"2015","journal-title":"Polym. Chem."},{"key":"10.1016\/j.bios.2020.112774_bib21","doi-asserted-by":"crossref","DOI":"10.1088\/1361-6528\/aaabea","article-title":"Carbon dots based immunosorbent assay for the determination of GFAP in human serum","volume":"29","author":"Ma","year":"2018","journal-title":"Nanotechnology"},{"key":"10.1016\/j.bios.2020.112774_bib22","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.bios.2015.11.033","article-title":"Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM sensor","volume":"78","author":"Masdor","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2020.112774_bib23","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jns.2005.09.007","article-title":"Increased serum-GFAP in patients with severe traumatic brain injury is related to outcome","volume":"240","author":"Nyl\u00e9n","year":"2006","journal-title":"J. Neurol. Sci."},{"key":"10.1016\/j.bios.2020.112774_bib24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/bs.mie.2019.02.006","article-title":"Visualizing the brain's astrocytes","volume":"622","author":"Preston","year":"2019","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bios.2020.112774_bib25","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1021\/ac3035426","article-title":"Half antibody fragments improve biosensor sensitivity without loss of selectivity","volume":"85","author":"Sharma","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2020.112774_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201501130","article-title":"Rapid and controllable digital microfluidic heating by surface acoustic waves","volume":"25","author":"Shilton","year":"2015","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.bios.2020.112774_bib27","doi-asserted-by":"crossref","first-page":"4941","DOI":"10.1002\/adma.201400091","article-title":"Nanoliter-droplet acoustic streaming via ultra high frequency surface acoustic waves","volume":"26","author":"Shilton","year":"2014","journal-title":"Adv. Mater."},{"key":"10.1016\/j.bios.2020.112774_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201606506","article-title":"Extended solution gate OFET-based biosensor for label-free glial fibrillary acidic protein detection with polyethylene glycol-containing bioreceptor layer","volume":"27","author":"Song","year":"2017","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.bios.2020.112774_bib29","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1080\/15548627.2019.1603549","article-title":"FBS\/BSA media concentration determines CCCP's ability to depolarize mitochondria and activate PINK1-PRKN mitophagy","volume":"15","author":"Soutar","year":"2019","journal-title":"Autophagy"},{"key":"10.1016\/j.bios.2020.112774_bib30","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1212\/WNL.0b013e3181e2414b","article-title":"Astrocytic damage is far more severe than demyelination in NMO: a clinical CSF biomarker study","volume":"75","author":"Takano","year":"2010","journal-title":"Neurology"},{"key":"10.1016\/j.bios.2020.112774_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss6609a1","article-title":"Traumatic brain injury\u2013related emergency department visits, hospitalizations, and deaths \u2014 United States, 2007 and 2013","volume":"66","author":"Taylor","year":"2017","journal-title":"MMWR Surveillance Summaries"},{"key":"10.1016\/j.bios.2020.112774_bib32","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s11060-015-1978-8","article-title":"Prospective evaluation of serum glial fibrillary acidic protein (GFAP) as a diagnostic marker for glioblastoma","volume":"126","author":"Tichy","year":"2016","journal-title":"J. Neuro Oncol."},{"key":"10.1016\/j.bios.2020.112774_bib33","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1039\/c2lc40396j","article-title":"Interaction-free, automatic, on-chip fluid routing by surface acoustic waves","volume":"12","author":"Travagliati","year":"2012","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2020.112774_bib34","doi-asserted-by":"crossref","first-page":"10633","DOI":"10.1021\/ac502465s","article-title":"Acoustofluidics and whole-blood manipulation in surface acoustic wave counterflow devices","volume":"86","author":"Travagliati","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2020.112774_bib35","author":"UniProt database"},{"key":"10.1016\/j.bios.2020.112774_bib36","doi-asserted-by":"crossref","first-page":"3927","DOI":"10.1007\/s00216-015-8606-5","article-title":"Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications","volume":"407","author":"Vaisocherov\u00e1","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.bios.2020.112774_bib37","doi-asserted-by":"crossref","first-page":"12685","DOI":"10.1021\/jp0481005","article-title":"Effect of film thickness on the validity of the sauerbrey equation for hydrated polyelectrolyte films","volume":"108","author":"Vogt","year":"2004","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bios.2020.112774_bib38","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1212\/01.WNL.0000120550.00643.DC","article-title":"Glial and neuronal proteins in serum predict outcome after severe traumatic brain injury","volume":"62","author":"Vos","year":"2004","journal-title":"Neurology"},{"key":"10.1016\/j.bios.2020.112774_bib39","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.20964\/2017.08.69","article-title":"Electrochemical quantitative detection of glial fibrillary acidic protein based on molecularly imprinted polymer sensor","volume":"12","author":"Wang","year":"2017","journal-title":"International Journal of Electrochemical Science"},{"key":"10.1016\/j.bios.2020.112774_bib43","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/14737159.2018.1428089","article-title":"An update on diagnostic and prognostic biomarkers for traumatic brain injury","volume":"18","author":"Wang","year":"2018","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.bios.2020.112774_bib40","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/S1474-4422(19)30282-0","article-title":"Association between plasma GFAP concentrations and MRI abnormalities in patients with CT-negative traumatic brain injury in the TRACK-TBI cohort: a prospective multicentre study","volume":"18","author":"Yue","year":"2019","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.bios.2020.112774_bib41","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1007\/s10072-013-1541-3","article-title":"Serum glial fibrillary acidic protein as a biomarker for differentiating intracerebral hemorrhage and ischemic stroke in patients with symptoms of acute stroke: a systematic review and meta-analysis","volume":"34","author":"Zhang","year":"2013","journal-title":"Neurol. Sci."},{"key":"10.1016\/j.bios.2020.112774_bib42","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1002\/smll.200700006","article-title":"Accurate immobilization of antibody-functionalized peptide nanotubes on protein-patterned arrays by optimizing their ligand-receptor interactions","volume":"3","author":"Zhao","year":"2007","journal-title":"Small"}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566320307612?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566320307612?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T01:13:08Z","timestamp":1760663588000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566320307612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":43,"alternative-id":["S0956566320307612"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2020.112774","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Glial-fibrillary-acidic-protein (GFAP) biomarker detection in serum-matrix: Functionalization strategies and detection by an ultra-high-frequency surface-acoustic-wave (UHF-SAW) lab-on-chip.","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2020.112774","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112774"}}