{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:01:24Z","timestamp":1792789284550,"version":"4.5.0"},"reference-count":58,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"am","delay-in-days":278,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R33AI147419"],"award-info":[{"award-number":["R33AI147419"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R33HD105610"],"award-info":[{"award-number":["R33HD105610"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["2319913"],"award-info":[{"award-number":["2319913"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["1912410"],"award-info":[{"award-number":["1912410"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["1902503"],"award-info":[{"award-number":["1902503"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.bios.2024.116997","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T06:48:21Z","timestamp":1732603701000},"page":"116997","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Compact multiplex PCR device for HIV-1 and HIV-2 viral load determination from finger-prick whole blood in resource-limited settings"],"prefix":"10.1016","volume":"271","author":[{"given":"Tianyi","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Politza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Ahasan","family":"Ahamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneesh","family":"Kshirsagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0666-7127","authenticated-orcid":false,"given":"Yusheng","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8435-9672","authenticated-orcid":false,"given":"Weihua","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2024.116997_bib1","doi-asserted-by":"crossref","first-page":"145a","DOI":"10.1016\/j.bpj.2023.11.1001","volume":"123","author":"Ahamed","year":"2024","journal-title":"Biophys. J."},{"key":"10.1016\/j.bios.2024.116997_bib2","volume":"36","author":"Ahamed","year":"2024","journal-title":"Nanotechnology"},{"key":"10.1016\/j.bios.2024.116997_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115866","volume":"246","author":"Ahamed","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2024.116997_bib4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s13027-023-00513-y","volume":"18","author":"Akakpo","year":"2023","journal-title":"Infect. Agents Cancer"},{"key":"10.1016\/j.bios.2024.116997_bib5","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bios.2024.116997_bib6","volume":"14","author":"Bwana","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.bios.2024.116997_bib7","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1093\/cid\/cit104","volume":"56","author":"Charpentier","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.bios.2024.116997_bib8","series-title":"Biomedical Engineering Technologies, Methods in Molecular Biology","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/978-1-0716-1803-5_16","article-title":"Sample-to-Answer microfluidic nucleic acid testing (NAT) on lab-on-a-disc for malaria detection at point of need","author":"Choi","year":"2022"},{"key":"10.1016\/j.bios.2024.116997_bib9","series-title":"Biomedical Engineering Technologies, Methods in Molecular Biology","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/978-1-0716-1803-5_14","article-title":"An ultracompact real-time fluorescence loop-mediated isothermal amplification (LAMP) analyzer","author":"Choi","year":"2022"},{"key":"10.1016\/j.bios.2024.116997_bib10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.bios.2018.05.019","volume":"115","author":"Choi","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2024.116997_bib11","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1039\/C6LC01078D","volume":"16","author":"Choi","year":"2016","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2024.116997_bib12","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jviromet.2016.09.007","volume":"237","author":"Curtis","year":"2016","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.bios.2024.116997_bib13","volume":"7","author":"Curtis","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bios.2024.116997_bib14","doi-asserted-by":"crossref","first-page":"324","DOI":"10.15302\/J-ENG-2015072","volume":"1","author":"Damhorst","year":"2015","journal-title":"Engineering"},{"key":"10.1016\/j.bios.2024.116997_bib15","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1038\/s41591-021-01590-5","volume":"27","author":"Deeks","year":"2021","journal-title":"Nat. Med."},{"key":"10.1016\/j.bios.2024.116997_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2023.114872","volume":"324","author":"Diop-Ndiaye","year":"2024","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.bios.2024.116997_bib17","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s12887-023-03943-0","volume":"23","author":"Djiyou","year":"2023","journal-title":"BMC Pediatr."},{"key":"10.1016\/j.bios.2024.116997_bib18","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1097\/QAI.0000000000003037","volume":"91","author":"Dorward","year":"2022","journal-title":"JAIDS Journal of Acquired Immune Deficiency Syndromes"},{"key":"10.1016\/j.bios.2024.116997_bib19","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00097-18","volume":"32","author":"Drain","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.bios.2024.116997_bib20","unstructured":"Franklin\u00ae Real-Time PCR Thermocycler [WWW Document], n.d. . Biomeme, Inc. URL https:\/\/shop.biomeme.com\/franklin-real-time-pcr-thermocycler\/(accessed 8.3.24)."},{"key":"10.1016\/j.bios.2024.116997_bib21","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01986-20","volume":"95","author":"Gaebler","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.bios.2024.116997_bib22","volume":"e00619\u201323","author":"Guiraud","year":"2023","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.bios.2024.116997_bib23","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01922-18","volume":"57","author":"Jagodzinski","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.bios.2024.116997_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1002089","volume":"13","author":"Kilmarx","year":"2016","journal-title":"PLoS Med."},{"key":"10.1016\/j.bios.2024.116997_bib25","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.15585\/mmwr.mm6547a2","volume":"65","author":"Lecher","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"10.1016\/j.bios.2024.116997_bib26","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.snb.2016.01.073","volume":"229","author":"Liao","year":"2016","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2024.116997_bib27","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1039\/c1an00007a","volume":"136","author":"Liu","year":"2011","journal-title":"The Analyst"},{"key":"10.1016\/j.bios.2024.116997_bib28","article-title":"Fingerpick blood-based nucleic acid testing on A USB interfaced device towards HIV self-testing","author":"Liu","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2024.116997_bib29","volume":"209","author":"Liu","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2024.116997_bib30","author":"Liu","year":"2023","journal-title":"ACS Sens. acssensors.3c01819"},{"key":"10.1016\/j.bios.2024.116997_bib31","doi-asserted-by":"crossref","first-page":"6065","DOI":"10.1038\/s41467-020-19821-7","volume":"11","author":"Mancuso","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bios.2024.116997_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2019.104212","volume":"122","author":"Mariani","year":"2020","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.bios.2024.116997_bib33","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1039\/C6LC01239F","volume":"17","author":"Mauk","year":"2017","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2024.116997_bib34","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1046\/j.1471-8286.2003.00548.x","volume":"3","author":"Mogg","year":"2003","journal-title":"Mol. Ecol. Notes"},{"key":"10.1016\/j.bios.2024.116997_bib35","first-page":"5905","volume":"24","author":"Moranguinho","year":"2023","journal-title":"Indian J. Manag. Sci."},{"key":"10.1016\/j.bios.2024.116997_bib36","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.2165","volume":"31","author":"Newman","year":"2021","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.bios.2024.116997_bib37","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41378-022-00400-3","volume":"8","author":"Nguyen","year":"2022","journal-title":"Microsyst Nanoeng"},{"key":"10.1016\/j.bios.2024.116997_bib38","doi-asserted-by":"crossref","first-page":"10701","DOI":"10.1021\/acsnano.3c01917","volume":"17","author":"Nouri","year":"2023","journal-title":"ACS Nano"},{"key":"10.1016\/j.bios.2024.116997_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113012","volume":"178","author":"Nouri","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2024.116997_bib40","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1186\/s12889-022-13504-2","volume":"22","author":"Pham","year":"2022","journal-title":"BMC Publ. Health"},{"issue":"3","key":"10.1016\/j.bios.2024.116997_bib41","volume":"14","author":"Pham","year":"2017","journal-title":"AIDS Res. Ther."},{"key":"10.1016\/j.bios.2024.116997_bib42","doi-asserted-by":"crossref","first-page":"6580","DOI":"10.1021\/acs.analchem.8b00269","volume":"90","author":"Phillips","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2024.116997_bib43","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1039\/C9LC00506D","volume":"19","author":"Phillips","year":"2019","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2024.116997_bib44","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1039\/D3LC00545C","volume":"23","author":"Politza","year":"2023","journal-title":"Lab Chip"},{"key":"10.1016\/j.bios.2024.116997_bib45","series-title":"A Portable Centrifuge for Universal Nucleic Acid Extraction at the Point-of-Care","author":"Politza","year":"2024"},{"key":"10.1016\/j.bios.2024.116997_bib46","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1038\/s41598-024-56164-5","volume":"14","author":"Prakash","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bios.2024.116997_bib47","doi-asserted-by":"crossref","first-page":"4186","DOI":"10.1038\/s41467-023-39837-z","volume":"14","author":"Reeves","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bios.2024.116997_bib48","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1128\/JCM.00593-14","volume":"52","author":"Ritchie","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.bios.2024.116997_bib49","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/nm0696-625","volume":"2","author":"Saag","year":"1996","journal-title":"Nat. Med."},{"key":"10.1016\/j.bios.2024.116997_bib50","first-page":"278","article-title":"Acs biomater-","volume":"2","author":"Safavieh","year":"2016","journal-title":"Sci Eng"},{"key":"10.1016\/j.bios.2024.116997_bib51","doi-asserted-by":"crossref","first-page":"2370","DOI":"10.1021\/acssensors.2c01023","volume":"7","author":"Tang","year":"2022","journal-title":"ACS Sens."},{"key":"10.1016\/j.bios.2024.116997_bib52","series-title":"ACS Sensors","author":"Tang","year":"2022"},{"key":"10.1016\/j.bios.2024.116997_bib53","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0283602","volume":"18","author":"Tchounga","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.bios.2024.116997_bib54","first-page":"1133","article-title":"J infect dis","volume":"197","year":"2008"},{"key":"10.1016\/j.bios.2024.116997_bib55","doi-asserted-by":"crossref","first-page":"e668","DOI":"10.1016\/S2214-109X(21)00034-6","volume":"9","author":"Thomas","year":"2021","journal-title":"Lancet Global Health"},{"key":"10.1016\/j.bios.2024.116997_bib56","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0250942","volume":"16","author":"Tombuloglu","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.bios.2024.116997_bib57","series-title":"HIV Key Facts","year":"2022"},{"key":"10.1016\/j.bios.2024.116997_bib59","volume":"16","author":"Zhang","year":"2024","journal-title":"WIREs Nanomed Nanobiotechnol"}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566324010042?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566324010042?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T03:44:20Z","timestamp":1750131860000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566324010042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":58,"alternative-id":["S0956566324010042"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2024.116997","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Compact multiplex PCR device for HIV-1 and HIV-2 viral load determination from finger-prick whole blood in resource-limited settings","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2024.116997","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116997"}}