{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:17:12Z","timestamp":1789906632292,"version":"4.0.1"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004489","name":"Mitacs Inc","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.bios.2025.117337","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T23:36:47Z","timestamp":1741304207000},"page":"117337","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["A label-free point-of-care electrochemical biosensor for early and accurate detection of monkeypox"],"prefix":"10.1016","volume":"278","author":[{"given":"Ahmed A.","family":"Khorshed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2025.117337_bib1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.aca.2020.02.004","article-title":"DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli","volume":"1107","author":"Abdelrasoul","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.bios.2025.117337_bib2","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.3389\/fpubh.2021.728969","article-title":"Evaluation of rapid antigen tests using nasal samples to diagnose SARS-CoV-2 in symptomatic patients","volume":"9","author":"Alqahtani","year":"2022","journal-title":"Front. Public Health"},{"key":"10.1016\/j.bios.2025.117337_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2022.107218","article-title":"Construction of a highly sensitive immunosensor based on antibody immunoglobulin G\/3-(trimethoxysilyl) propylamine\/graphene oxide for antigen-specific immunoglobulin G detection","volume":"176","author":"Amini","year":"2022","journal-title":"Microchem. J."},{"issue":"24","key":"10.1016\/j.bios.2025.117337_bib4","doi-asserted-by":"crossref","first-page":"11438","DOI":"10.1021\/acs.nanolett.3c02709","article-title":"Selective single-molecule nanopore detection of mpox A29 protein directly in biofluids","volume":"23","author":"Cai","year":"2023","journal-title":"Nano Lett."},{"key":"10.1016\/j.bios.2025.117337_bib5","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.jcis.2023.12.188","article-title":"Fabrication of label-free immunoprobe for monkeypox A29 detection using one-step electrodeposited molybdenum oxide-graphene quantum rods","volume":"660","author":"Chandran","year":"2024","journal-title":"J. Colloid Interface Sci."},{"issue":"50","key":"10.1016\/j.bios.2025.117337_bib6","doi-asserted-by":"crossref","first-page":"58079","DOI":"10.1021\/acsami.3c10730","article-title":"Electrochemical paper-Based nanobiosensor for rapid and sensitive detection of monkeypox virus","volume":"15","author":"de Lima","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"9","key":"10.1016\/j.bios.2025.117337_bib7","doi-asserted-by":"crossref","first-page":"6065","DOI":"10.1021\/acsabm.4c00677","article-title":"Red fluorescent copper nanoclusters for fluorescence, smartphone, and electrochemical sensor arrays to detect the monkeypox A29 protein","volume":"7","author":"Dhanasekaran","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"issue":"3","key":"10.1016\/j.bios.2025.117337_bib8","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/S1473-3099(18)30043-4","article-title":"Genomic characterisation of human monkeypox virus in Nigeria","volume":"18","author":"Faye","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.bios.2025.117337_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2022.113538","article-title":"Recent advances in point of care testing for COVID-19 detection","volume":"153","author":"Fernandes","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.bios.2025.117337_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113112","article-title":"Electrochemical diagnostics of infectious viral diseases: trends and challenges","volume":"180","author":"Goud","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"10","key":"10.1016\/j.bios.2025.117337_bib11","doi-asserted-by":"crossref","first-page":"571","DOI":"10.3390\/bioengineering9100571","article-title":"Current and perspective sensing methods for monkeypox virus","volume":"9","author":"Gul","year":"2022","journal-title":"Bioengineering"},{"issue":"5","key":"10.1016\/j.bios.2025.117337_bib12","doi-asserted-by":"crossref","first-page":"264","DOI":"10.3390\/md21050264","article-title":"SPR sensor-based analysis of the inhibition of marine sulfated glycans on interactions between monkeypox virus proteins and glycosaminoglycans","volume":"21","author":"He","year":"2023","journal-title":"Mar. Drugs"},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib13","doi-asserted-by":"crossref","first-page":"244","DOI":"10.3201\/eid2801.211749","article-title":"Postmortem antigen-detecting rapid diagnostic tests to predict infectivity of SARS-CoV-2-associated deaths","volume":"28","author":"Heinrich","year":"2022","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib14","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1016\/S2666-5247(22)00291-9","article-title":"Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional study","volume":"4","author":"Hernaez","year":"2023","journal-title":"The Lancet Microbe"},{"key":"10.1016\/j.bios.2025.117337_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116725","article-title":"Lateral flow assays for viruses diagnosis: up-to-date technology and future prospects","volume":"157","author":"Ince","year":"2022","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"7","key":"10.1016\/j.bios.2025.117337_bib16","article-title":"Monkeypox: a comprehensive review of transmission, pathogenesis, and manifestation","volume":"14","author":"Kaler","year":"2022","journal-title":"Cureus"},{"key":"10.1016\/j.bios.2025.117337_bib17","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.snb.2016.07.165","article-title":"Sensitive determination of amlodipine besylate using bare\/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids","volume":"239","author":"Khairy","year":"2017","journal-title":"Sensor. Actuator. B Chem."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib18","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-17219-7","article-title":"A gold nanoparticle-protein G electrochemical affinity biosensor for the detection of SARS-CoV-2 antibodies: a surface modification approach","volume":"12","author":"Khaniani","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bios.2025.117337_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1088471","article-title":"The monkeypox diagnosis, treatments and prevention: a review","volume":"12","author":"Khattak","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.bios.2025.117337_bib20","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jelechem.2018.07.029","article-title":"Voltammetric determination of meclizine antihistamine drug utilizing graphite screen-printed electrodes in physiological medium","volume":"824","author":"Khorshed","year":"2018","journal-title":"J. Electroanal. Chem."},{"key":"10.1016\/j.bios.2025.117337_bib21","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.talanta.2019.01.117","article-title":"Electrochemical determination of antihypertensive drugs by employing costless and portable unmodified screen-printed electrodes","volume":"198","author":"Khorshed","year":"2019","journal-title":"Talanta"},{"issue":"3","key":"10.1016\/j.bios.2025.117337_bib22","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1039\/C8AY02405G","article-title":"Disposable screen-printed electrodes modified with uniform iron oxide nanocubes for the simple electrochemical determination of meclizine, an antihistamine drug","volume":"11","author":"Khorshed","year":"2019","journal-title":"Anal. Methods"},{"key":"10.1016\/j.bios.2025.117337_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115793","article-title":"Development of an impedance-based biosensor for determination of IgG galactosylation levels","volume":"245","author":"Khorshed","year":"2024","journal-title":"Biosens. Bioelectron."},{"issue":"14","key":"10.1016\/j.bios.2025.117337_bib24","doi-asserted-by":"crossref","first-page":"7866","DOI":"10.3390\/ijms23147866","article-title":"Monkeypox: a new threat?","volume":"23","author":"Kmiec","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"10.1016\/j.bios.2025.117337_bib25","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.jmii.2022.07.004","article-title":"Monkeypox: an emerging global threat during the COVID-19 pandemic","volume":"55","author":"Lai","year":"2022","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.bios.2025.117337_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2022.198932","article-title":"The virology of human monkeypox virus (hMPXV): a brief overview","volume":"322","author":"Lansiaux","year":"2022","journal-title":"Virus Res."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib27","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1021\/acs.analchem.5b02367","article-title":"Hampering of the stability of gold electrodes by ferri-\/ferrocyanide redox couple electrolytes during electrochemical impedance spectroscopy","volume":"88","author":"Lazar","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2025.117337_bib28","doi-asserted-by":"crossref","first-page":"11318","DOI":"10.1039\/D4NR01618A","article-title":"CRISPR\/Cas12a antifouling nanocomposite electrochemical biosensors enable amplification-free detection of Monkeypox virus in complex biological fluids","volume":"16","author":"Lee","year":"2024","journal-title":"Nanoscale"},{"issue":"4","key":"10.1016\/j.bios.2025.117337_bib29","doi-asserted-by":"crossref","DOI":"10.1002\/INMD.20230020","article-title":"Nanozymes: applications in clinical biomarker detection","volume":"1","author":"Liang","year":"2023","journal-title":"Interdiscip. Med."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib30","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.28429","article-title":"Mpox diagnostics: review of current and emerging technologies","volume":"95","author":"Lim","year":"2023","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.bios.2025.117337_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2023.123087","article-title":"Label-free detection of virus based on surface-enhanced Raman scattering","volume":"302","author":"Lv","year":"2023","journal-title":"Spectrochim. Acta Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.bios.2025.117337_bib32","article-title":"Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation","volume":"11","author":"Ma","year":"2022","journal-title":"Biosens. Bioelectron. X"},{"key":"10.1016\/j.bios.2025.117337_bib33","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jviromet.2016.07.024","article-title":"Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay","volume":"236","author":"Maksyutov","year":"2016","journal-title":"J. Virol. Methods"},{"issue":"20","key":"10.1016\/j.bios.2025.117337_bib34","doi-asserted-by":"crossref","first-page":"7149","DOI":"10.1021\/acs.analchem.1c05358","article-title":"Recent advances in electrochemical tools for virus detection","volume":"94","author":"Manring","year":"2022","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib35","doi-asserted-by":"crossref","first-page":"4","DOI":"10.3390\/sci4010004","article-title":"A practical approach for quantitative polymerase chain reaction, the gold standard in microbiological diagnosis","volume":"4","author":"Mosolyg\u00f3","year":"2022","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib36","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1749-6632.1993.tb18343.x","article-title":"Methods for collecting saliva","volume":"694","author":"Navazesh","year":"1993","journal-title":"Ann. N. Y. Acad. Sci."},{"issue":"9","key":"10.1016\/j.bios.2025.117337_bib37","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.3201\/eid2809.220917","article-title":"Rapid adaptation of established high-throughput molecular testing infrastructure for monkeypox virus detection","volume":"28","author":"N\u00f6rz","year":"2022","journal-title":"Emerg. Infect. Dis."},{"issue":"6","key":"10.1016\/j.bios.2025.117337_bib38","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1021\/acs.analchem.1c04296","article-title":"Capacitive aptasensor coupled with microfluidic enrichment for real-time detection of trace SARS-CoV-2 nucleocapsid protein","volume":"94","author":"Qi","year":"2022","journal-title":"Anal. Chem."},{"issue":"5","key":"10.1016\/j.bios.2025.117337_bib39","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.3390\/v15051120","article-title":"Development of a multi-epitope universal mRNA vaccine candidate for monkeypox, smallpox, and Vaccinia viruses: design and in silico analyses","volume":"15","author":"Rcheulishvili","year":"2023","journal-title":"Viruses"},{"issue":"8","key":"10.1016\/j.bios.2025.117337_bib40","doi-asserted-by":"crossref","first-page":"924","DOI":"10.3390\/pathogens11080924","article-title":"Monkeypox and its Possible Sexual Transmission: where are we now with its evidence?","volume":"11","author":"Sah","year":"2022","journal-title":"Pathogens"},{"issue":"6","key":"10.1016\/j.bios.2025.117337_bib41","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1021\/acssensors.0c00604","article-title":"Epidermal enzymatic biosensors for sweat vitamin C: toward personalized nutrition","volume":"5","author":"Sempionatto","year":"2020","journal-title":"ACS Sens."},{"issue":"7","key":"10.1016\/j.bios.2025.117337_bib42","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/s41551-021-00685-1","article-title":"An epidermal patch for the simultaneous monitoring of haemodynamic and metabolic biomarkers","volume":"5","author":"Sempionatto","year":"2021","journal-title":"Nat. Biomed. Eng."},{"issue":"18","key":"10.1016\/j.bios.2025.117337_bib43","doi-asserted-by":"crossref","first-page":"5898","DOI":"10.3390\/molecules27185898","article-title":"Kinetic and structural aspects of glycosaminoglycan\u2013monkeypox virus protein A29 interactions using surface plasmon resonance","volume":"27","author":"Shi","year":"2022","journal-title":"Molecules"},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib44","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41378-022-00460-5","article-title":"Label-free impedimetric immunosensor for point-of-care detection of COVID-19 antibodies","volume":"9","author":"Shoute","year":"2023","journal-title":"Microsyst. Nanoeng."},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib45","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/bios14010006","article-title":"Faradaic impedimetric immunosensor for label-free point-of-care detection of COVID-19 antibodies using gold-interdigitated electrode array","volume":"14","author":"Shoute","year":"2023","journal-title":"Biosensors"},{"key":"10.1016\/j.bios.2025.117337_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116727","article-title":"Recent trends and advancements in electrochemiluminescence biosensors for human virus detection","volume":"157","author":"Sobhanie","year":"2022","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"5","key":"10.1016\/j.bios.2025.117337_bib47","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1039\/D2AN02043B","article-title":"Gold-based paper for antigen detection of monkeypox virus","volume":"148","author":"Ye","year":"2023","journal-title":"Analyst"},{"key":"10.1016\/j.bios.2025.117337_bib48","article-title":"A portable, low-cost and high-throughput electrochemical impedance spectroscopy device for point-of-care biomarker detection","volume":"13","author":"Ye","year":"2022","journal-title":"Biosens. Bioelectron. X"},{"issue":"1","key":"10.1016\/j.bios.2025.117337_bib49","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3390\/v15010084","article-title":"Development of a novel loop-mediated isothermal amplification method for the rapid detection of monkeypox virus infections","volume":"15","author":"Yu","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.bios.2025.117337_bib50","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114476","article-title":"An impedimetric biosensor for COVID-19 serology test and modification of sensor performance via dielectrophoresis force","volume":"213","author":"Zeng","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"17","key":"10.1016\/j.bios.2025.117337_bib51","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1039\/D2AY01998A","article-title":"CRISPR\/Cas12a-mediated ultrasensitive and on-site monkeypox viral testing","volume":"15","author":"Zhao","year":"2023","journal-title":"Anal. Methods"},{"issue":"7","key":"10.1016\/j.bios.2025.117337_bib52","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/S1473-3099(22)00354-1","article-title":"Monkeypox outbreaks outside endemic regions: scientific and social priorities","volume":"22","author":"Zumla","year":"2022","journal-title":"Lancet Infect. Dis."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325002118?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325002118?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T19:05:33Z","timestamp":1744311933000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566325002118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":52,"alternative-id":["S0956566325002118"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2025.117337","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A label-free point-of-care electrochemical biosensor for early and accurate detection of monkeypox","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2025.117337","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117337"}}