{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:16:09Z","timestamp":1793315769498,"version":"4.5.0"},"reference-count":58,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2400902"],"award-info":[{"award-number":["2021YFC2400902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62375217"],"award-info":[{"award-number":["62375217"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272048"],"award-info":[{"award-number":["82272048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.bios.2025.117428","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T11:00:30Z","timestamp":1743591630000},"page":"117428","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["A rapid and ultrasensitive RPA-assisted CRISPR\u2013Cas12a\/Cas13a nucleic acid diagnostic platform with a smartphone-based portable device"],"prefix":"10.1016","volume":"280","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5898-6393","authenticated-orcid":false,"given":"Fei","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhui","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6659-6888","authenticated-orcid":false,"given":"Fangning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xincheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7811","key":"10.1016\/j.bios.2025.117428_bib1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/s41586-020-2279-8","article-title":"Massively multiplexed nucleic acid detection with Cas13","volume":"582","author":"Ackerman","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.bios.2025.117428_bib2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/bs.pmbts.2020.12.012","article-title":"History, evolution and classification of CRISPR-Cas associated systems","volume":"179","author":"Agarwal","year":"2021","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"10.1016\/j.bios.2025.117428_bib3","article-title":"iSCAN-V2: a one-pot RT-RPA-CRISPR\/cas12b assay for point-of-care SARS-CoV-2 detection","volume":"9","author":"Aman","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"8","key":"10.1016\/j.bios.2025.117428_bib4","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/s41551-022-00889-z","article-title":"Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants","volume":"6","author":"Arizti-Sanz","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib5","doi-asserted-by":"crossref","first-page":"5921","DOI":"10.1038\/s41467-020-19097-x","article-title":"Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2","volume":"11","author":"Arizti-Sanz","year":"2020","journal-title":"Nat. Commun."},{"issue":"70","key":"10.1016\/j.bios.2025.117428_bib6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3126\/kumj.v18i2.33035","article-title":"Virology, clinical features and diagnosis of COVID 19: review analysis","volume":"18","author":"Bhusal","year":"2020","journal-title":"Kathmandu Univ. Med. J."},{"issue":"2","key":"10.1016\/j.bios.2025.117428_bib7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1134\/S0006297920020030","article-title":"Isothermal nucleic acid amplification techniques and their use in bioanalysis","volume":"85","author":"Bodulev","year":"2020","journal-title":"Biochemistry (Mosc)"},{"issue":"9","key":"10.1016\/j.bios.2025.117428_bib8","doi-asserted-by":"crossref","DOI":"10.3390\/bios12090677","article-title":"Isothermal amplification technology for disease diagnosis","volume":"12","author":"Boonbanjong","year":"2022","journal-title":"Biosensors"},{"issue":"7","key":"10.1016\/j.bios.2025.117428_bib9","doi-asserted-by":"crossref","DOI":"10.3390\/mi8070228","article-title":"Rapid nucleic acid extraction and purification using a miniature ultrasonic technique","volume":"8","author":"Branch","year":"2017","journal-title":"Micromachines"},{"issue":"7","key":"10.1016\/j.bios.2025.117428_bib10","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR-Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bios.2025.117428_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112887","article-title":"CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics","volume":"177","author":"Bruch","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"8","key":"10.1016\/j.bios.2025.117428_bib12","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.tibtech.2019.04.005","article-title":"CRISPR\/Cas powered multiplexed biosensing","volume":"37","author":"Bruch","year":"2019","journal-title":"Trends Biotechnol."},{"issue":"9","key":"10.1016\/j.bios.2025.117428_bib13","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1080\/14737159.2020.1816466","article-title":"New and developing diagnostic platforms for COVID-19: a systematic review","volume":"20","author":"Chauhan","year":"2020","journal-title":"Expert Rev. Mol. Diagn"},{"issue":"6387","key":"10.1016\/j.bios.2025.117428_bib14","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"21","key":"10.1016\/j.bios.2025.117428_bib15","doi-asserted-by":"crossref","first-page":"14846","DOI":"10.1021\/acs.analchem.0c03883","article-title":"Reagents-loaded, automated assay that integrates recombinase-aided amplification and Cas12a nucleic acid detection for a point-of-care test","volume":"92","author":"Chen","year":"2020","journal-title":"Anal. Chem."},{"issue":"5\u20136","key":"10.1016\/j.bios.2025.117428_bib16","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1159\/000507423","article-title":"COVID-19 outbreak: an overview","volume":"64","author":"Ciotti","year":"2019","journal-title":"Chemotherapy"},{"key":"10.1016\/j.bios.2025.117428_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114101","article-title":"Isothermal amplification-assisted diagnostics for COVID-19","volume":"205","author":"De Felice","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"8","key":"10.1016\/j.bios.2025.117428_bib18","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.tibtech.2017.03.013","article-title":"Multiplexed point-of-care testing - xPOCT","volume":"35","author":"Dincer","year":"2017","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.bios.2025.117428_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.639108","article-title":"CRISPR\/Cas system: a potential technology for the prevention and control of COVID-19 and emerging infectious diseases","volume":"11","author":"Ding","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib20","doi-asserted-by":"crossref","first-page":"4711","DOI":"10.1038\/s41467-020-18575-6","article-title":"Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay","volume":"11","author":"Ding","year":"2020","journal-title":"Nat. Commun."},{"issue":"41","key":"10.1016\/j.bios.2025.117428_bib21","doi-asserted-by":"crossref","first-page":"6108","DOI":"10.1039\/D2CC01297A","article-title":"A smartphone-based three-in-one biosensor for co-detection of SARS-CoV-2 viral RNA, antigen and antibody","volume":"58","author":"Dou","year":"2022","journal-title":"Chem. Commun."},{"issue":"2","key":"10.1016\/j.bios.2025.117428_bib22","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.cell.2020.12.001","article-title":"Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy","volume":"184","author":"Fozouni","year":"2021","journal-title":"Cell"},{"issue":"6387","key":"10.1016\/j.bios.2025.117428_bib23","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"issue":"6336","key":"10.1016\/j.bios.2025.117428_bib24","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.bios.2025.117428_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.113994","article-title":"A one-pot CRISPR\/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics","volume":"202","author":"Hu","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"23","key":"10.1016\/j.bios.2025.117428_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202300663","article-title":"Light-start CRISPR-cas12a reaction with caged crRNA enables rapid and sensitive nucleic acid detection","volume":"62","author":"Hu","year":"2023","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"26","key":"10.1016\/j.bios.2025.117428_bib27","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2202034119","article-title":"Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics","volume":"119","author":"Hu","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"8","key":"10.1016\/j.bios.2025.117428_bib28","doi-asserted-by":"crossref","DOI":"10.3390\/v12080863","article-title":"SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP","volume":"12","author":"Klein","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.bios.2025.117428_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2020.104433","article-title":"Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories","volume":"128","author":"LeBlanc","year":"2020","journal-title":"J. Clin. Virol."},{"issue":"10","key":"10.1016\/j.bios.2025.117428_bib30","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acssynbio.9b00209","article-title":"HOLMESv2: a CRISPR-cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation","volume":"8","author":"Li","year":"2019","journal-title":"ACS Synth. Biol."},{"issue":"4","key":"10.1016\/j.bios.2025.117428_bib31","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/s41422-018-0022-x","article-title":"CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA","volume":"28","author":"Li","year":"2018","journal-title":"Cell Res."},{"key":"10.1016\/j.bios.2025.117428_bib32","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted nucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell. Discov."},{"issue":"23","key":"10.1016\/j.bios.2025.117428_bib33","doi-asserted-by":"crossref","first-page":"8277","DOI":"10.1021\/acs.analchem.2c00616","article-title":"Glycerol additive boosts 100-fold sensitivity enhancement for one-pot RPA-CRISPR\/Cas12a assay","volume":"94","author":"Lin","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2025.117428_bib34","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.trac.2017.10.015","article-title":"Recombinase polymerase amplification: basics, applications and recent advances","volume":"98","author":"Lobato","year":"2018","journal-title":"Trends Anal. Chem."},{"issue":"3","key":"10.1016\/j.bios.2025.117428_bib35","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/s41551-022-00861-x","article-title":"Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a","volume":"6","author":"Lu","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"6","key":"10.1016\/j.bios.2025.117428_bib36","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrmicro2577","article-title":"Evolution and classification of the CRISPR-Cas systems","volume":"9","author":"Makarova","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"issue":"6387","key":"10.1016\/j.bios.2025.117428_bib37","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable viral diagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"issue":"8","key":"10.1016\/j.bios.2025.117428_bib38","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1038\/s41551-022-00919-w","article-title":"A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma","volume":"6","author":"Najjar","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"24","key":"10.1016\/j.bios.2025.117428_bib39","doi-asserted-by":"crossref","first-page":"16204","DOI":"10.1021\/acs.analchem.0c04047","article-title":"Isothermal amplification and ambient visualization in a single tube for the detection of SARS-CoV-2 using loop-mediated amplification and CRISPR technology","volume":"92","author":"Pang","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2025.117428_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112592","article-title":"Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases","volume":"169","author":"Paul","year":"2020","journal-title":"Biosens. Bioelectron."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib41","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s41664-022-00212-4","article-title":"Improved strategies for CRISPR-cas12-based nucleic acids detection","volume":"6","author":"Qiu","year":"2022","journal-title":"J. Anal. Test."},{"issue":"39","key":"10.1016\/j.bios.2025.117428_bib42","doi-asserted-by":"crossref","first-page":"24450","DOI":"10.1073\/pnas.2011221117","article-title":"SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification","volume":"117","author":"Rabe","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"5","key":"10.1016\/j.bios.2025.117428_bib43","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics11050813","article-title":"Analytical evaluation of Visby medical RT-PCR portable device for rapid detection of SARS-CoV-2","volume":"11","author":"Renzoni","year":"2021","journal-title":"Diagnostics"},{"issue":"3","key":"10.1016\/j.bios.2025.117428_bib44","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00228-20","article-title":"Tools and techniques for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)\/COVID-19 detection","volume":"34","author":"Safiabadi Tali","year":"2021","journal-title":"Clin. Microbiol. Rev."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib45","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s10544-006-9015-5","article-title":"A disposable lab-on-a-chip platform with embedded fluid actuators for active nanoliter liquid handling","volume":"9","author":"Samel","year":"2007","journal-title":"Biomed. Microdevices"},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib46","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s13073-018-0543-4","article-title":"Pathogen detection in the CRISPR-Cas era","volume":"10","author":"Sashital","year":"2018","journal-title":"Genome Med."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib47","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1186\/s13059-019-1742-z","article-title":"CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity","volume":"20","author":"Teng","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.bios.2025.117428_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113701","article-title":"Exploiting the orthogonal CRISPR-Cas12a\/Cas13a trans-cleavage for dual-gene virus detection using a handheld device","volume":"196","author":"Tian","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib49","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1146\/annurev-anchem-090822-014725","article-title":"CRISPR-based biosensing strategies: technical development and application prospects","volume":"16","author":"Tian","year":"2023","journal-title":"Annu. Rev. Anal. Chem."},{"issue":"7","key":"10.1016\/j.bios.2025.117428_bib50","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/s41589-023-01534-9","article-title":"Fast and sensitive CRISPR detection by minimized interference of target amplification","volume":"20","author":"Tong","year":"2024","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bios.2025.117428_bib51","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2023.341258","article-title":"CRISPR gel: a one-pot biosensing platform for rapid and sensitive detection of HIV viral RNA","volume":"1262","author":"Uno","year":"2023","journal-title":"Anal. Chim. Acta"},{"issue":"19","key":"10.1016\/j.bios.2025.117428_bib52","doi-asserted-by":"crossref","first-page":"12156","DOI":"10.1021\/acs.analchem.9b01526","article-title":"Cas12aVDet: a CRISPR\/cas12a-based platform for rapid and visual nucleic acid detection","volume":"91","author":"Wang","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2025.117428_bib53","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112766","article-title":"opvCRISPR: one-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection","volume":"172","author":"Wang","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"7","key":"10.1016\/j.bios.2025.117428_bib54","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1021\/acssynbio.3c00089","article-title":"Development of a naked eye CRISPR-cas12a and -Cas13a multiplex point-of-care detection of genetically modified swine","volume":"12","author":"Wang","year":"2023","journal-title":"ACS Synth. Biol."},{"issue":"4","key":"10.1016\/j.bios.2025.117428_bib55","doi-asserted-by":"crossref","DOI":"10.3390\/mi14040830","article-title":"A lab-on-a-tube biosensor combining recombinase-aided amplification and CRISPR-cas12a with rotated magnetic extraction for Salmonella detection","volume":"14","author":"Wu","year":"2023","journal-title":"Micromachines"},{"issue":"9","key":"10.1016\/j.bios.2025.117428_bib56","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1080\/14737159.2020.1816171","article-title":"The diagnostic methods in the COVID-19 pandemic, today and in the future","volume":"20","author":"Wu","year":"2020","journal-title":"Expert Rev. Mol. Diagn"},{"issue":"1","key":"10.1016\/j.bios.2025.117428_bib57","doi-asserted-by":"crossref","first-page":"6480","DOI":"10.1038\/s41467-022-34086-y","article-title":"Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification","volume":"13","author":"Xu","year":"2022","journal-title":"Nat. Commun."},{"issue":"12","key":"10.1016\/j.bios.2025.117428_bib58","doi-asserted-by":"crossref","first-page":"8561","DOI":"10.1021\/acs.analchem.0c01459","article-title":"Dynamic aqueous multiphase reaction system for one-pot CRISPR-cas12a-based ultrasensitive and quantitative molecular diagnosis","volume":"92","author":"Yin","year":"2020","journal-title":"Anal. Chem."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325003021?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325003021?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T02:17:36Z","timestamp":1745201856000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566325003021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":58,"alternative-id":["S0956566325003021"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2025.117428","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid and ultrasensitive RPA-assisted CRISPR\u2013Cas12a\/Cas13a nucleic acid diagnostic platform with a smartphone-based portable device","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2025.117428","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117428"}}