{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:49:41Z","timestamp":1792680581892,"version":"4.5.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100019341","name":"Shenzhen Scientific and Technological Foundation","doi-asserted-by":"publisher","award":["RCJC20221008092726022"],"award-info":[{"award-number":["RCJC20221008092726022"]}],"id":[{"id":"10.13039\/501100019341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019341","name":"Shenzhen Scientific and Technological Foundation","doi-asserted-by":"publisher","award":["KCXFZ20211020163545004"],"award-info":[{"award-number":["KCXFZ20211020163545004"]}],"id":[{"id":"10.13039\/501100019341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370012"],"award-info":[{"award-number":["82370012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0907201"],"award-info":[{"award-number":["2020YFA0907201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019530","name":"Science Fund for Distinguished Young Scholars of Gansu Province","doi-asserted-by":"publisher","award":["0620220214"],"award-info":[{"award-number":["0620220214"]}],"id":[{"id":"10.13039\/501100019530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018540","name":"Science Fund for Distinguished Young Scholars of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzen Municipality","doi-asserted-by":"publisher","award":["SZZYSM202311009"],"award-info":[{"award-number":["SZZYSM202311009"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.bios.2025.117566","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T02:28:22Z","timestamp":1746757702000},"page":"117566","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6\/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)"],"prefix":"10.1016","volume":"284","author":[{"given":"Siyuan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhao","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1683-3993","authenticated-orcid":false,"given":"Su","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.bios.2025.117566_bib1","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1080\/10408398.2018.1564234","article-title":"Aptamers: an emerging class of bioaffinity ligands in bioactive peptide applications","volume":"60","author":"Acquah","year":"2020","journal-title":"Crit. Rev. Food. Sci."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib2","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1080\/22221751.2019.1664939","article-title":"CRISPR-Based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis","volume":"8","author":"Ai","year":"2019","journal-title":"Emerg. Microb. Infect."},{"issue":"6","key":"10.1016\/j.bios.2025.117566_bib3","doi-asserted-by":"crossref","first-page":"968","DOI":"10.3390\/biom13060968","article-title":"ESAT-6 a major virulence factor of Mycobacterium tuberculosis","volume":"13","author":"Anes","year":"2023","journal-title":"Biomolecules"},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/S2666-5247(22)00359-7","article-title":"WHO's global tuberculosis report 2022","volume":"4","author":"Bagcchi","year":"2023","journal-title":"Lancet Microbe"},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib5","article-title":"Recent efforts in the development of glycoconjugate vaccine and available treatment for tuberculosis","volume":"150","author":"Banoo","year":"2024","journal-title":"Bioogr. Chem."},{"issue":"6360","key":"10.1016\/j.bios.2025.117566_bib6","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/355564a0","article-title":"Selection of single-stranded DNA molecules that bind and inhibit human thrombin","volume":"355","author":"Bock","year":"1992","journal-title":"Nature"},{"issue":"11","key":"10.1016\/j.bios.2025.117566_bib7","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"Boehme","year":"2010","journal-title":"N. Engl. J. Med."},{"issue":"9776","key":"10.1016\/j.bios.2025.117566_bib8","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/S0140-6736(11)60438-8","article-title":"Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the xpert MTB\/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study","volume":"377","author":"Boehme","year":"2011","journal-title":"Lancet"},{"issue":"7","key":"10.1016\/j.bios.2025.117566_bib9","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR-Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"issue":"5","key":"10.1016\/j.bios.2025.117566_bib10","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1086\/342293","article-title":"Detection of antibody to Mycobacterium tuberculosis protein antigens in the cerebrospinal fluid of patients with tuberculous meningitis","volume":"186","author":"Chandramuki","year":"2002","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib11","doi-asserted-by":"crossref","first-page":"4617","DOI":"10.2147\/IDR.S373652","article-title":"A peptidomic approach to identify novel antigen biomarkers for the diagnosis of tuberculosis","volume":"15","author":"Chen","year":"2022","journal-title":"Infect. Drug Resist."},{"issue":"2","key":"10.1016\/j.bios.2025.117566_bib12","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1183\/09031936.00007814","article-title":"Xpert MTB\/RIF assay for the diagnosis of extrapulmonary tuberculosis: a systematic review and meta-analysis","volume":"44","author":"Denkinger","year":"2014","journal-title":"Eur. Respir. J."},{"issue":"8","key":"10.1016\/j.bios.2025.117566_bib13","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/s41579-018-0013-4","article-title":"Metabolic principles of persistence and pathogenicity in Mycobacterium tuberculosis","volume":"16","author":"Ehrt","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"issue":"6336","key":"10.1016\/j.bios.2025.117566_bib14","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.bios.2025.117566_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.807095","article-title":"Whole genome sequencing identifies novel mutations associated with bedaquiline resistance in Mycobacterium tuberculosis","volume":"12","author":"Guo","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"5","key":"10.1016\/j.bios.2025.117566_bib16","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.00167-2021","article-title":"Tests for tuberculosis infection: landscape analysis","volume":"58","author":"Hamada","year":"2021","journal-title":"Eur. Respir. J."},{"issue":"15","key":"10.1016\/j.bios.2025.117566_bib17","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1056\/NEJMc2026172","article-title":"Detection of SARS-CoV-2 with SHERLOCK one-pot testing","volume":"383","author":"Joung","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.bios.2025.117566_bib18","doi-asserted-by":"crossref","first-page":"8911","DOI":"10.1038\/ncomms9911","article-title":"Chemical basis for the recognition of trimethyllysine by epigenetic reader proteins","volume":"6","author":"Kamps","year":"2015","journal-title":"Nat. Commun."},{"issue":"21","key":"10.1016\/j.bios.2025.117566_bib19","doi-asserted-by":"crossref","first-page":"8966","DOI":"10.1021\/ac302902s","article-title":"Aptamer-based viability impedimetric sensor for bacteria","volume":"84","author":"Labib","year":"2012","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib20","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1039\/C6AN02109C","article-title":"Importance of specimen pretreatment for the low-level detection of mycobacterial lipoarabinomannan in human serum","volume":"142","author":"Laurentius","year":"2016","journal-title":"Analyst"},{"issue":"543","key":"10.1016\/j.bios.2025.117566_bib21","article-title":"Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)\/terephthalate metal-organic framework","volume":"185","author":"Li","year":"2018","journal-title":"Microchim. Acta"},{"issue":"10","key":"10.1016\/j.bios.2025.117566_bib22","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1021\/acssynbio.1c00103","article-title":"Highly sensitive CRISPR\/Cas12a-Based fluorescence detection of porcine reproductive and respiratory syndrome virus","volume":"10","author":"Liu","year":"2021","journal-title":"ACS Synth. Biol."},{"issue":"10","key":"10.1016\/j.bios.2025.117566_bib23","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1007\/s10096-020-03951-3","article-title":"Screening and evaluation of Mycobacterium tuberculosis diagnostic antigens","volume":"39","author":"Ma","year":"2020","journal-title":"Eur. J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.bios.2025.117566_bib24","article-title":"Advances in molecular diagnosis of tuberculosis","volume":"58","author":"MacLean","year":"2020","journal-title":"J. Clin. Microbol."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1128\/CMR.00034-13","article-title":"Gamma interferon release assays for detection of Mycobacterium tuberculosis infection","volume":"27","author":"Pai","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.bios.2025.117566_bib26","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1136\/thorax-2023-220647","article-title":"TB PCR in BAL and EBUS-TBNA samples for the diagnosis of pulmonary and mediastinal lymph node TB: retrospective TRiBE study","volume":"79","author":"Park","year":"2024","journal-title":"Thorax"},{"key":"10.1016\/j.bios.2025.117566_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.126769","article-title":"Exploring recombinant secretory proteins from Mycobacterium tuberculosis to develop a serological platform for tuberculosis diagnosis","volume":"249","author":"Ramalingam","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"5","key":"10.1016\/j.bios.2025.117566_bib28","doi-asserted-by":"crossref","first-page":"231","DOI":"10.5588\/ijtld.23.0432","article-title":"Characteristics of TB cases without documented sputum culture in the United States, 2011-2021","volume":"28","author":"Rautman","year":"2024","journal-title":"Int. J. Tubercul. Lung Dis."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib29","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98471-1","article-title":"Detection and quantification of Mycobacterium tuberculosis antigen CFP10 in serum and urine for the rapid diagnosis of active tuberculosis disease","volume":"11","author":"Seifert","year":"2021","journal-title":"Sci. Rep."},{"issue":"12","key":"10.1016\/j.bios.2025.117566_bib30","doi-asserted-by":"crossref","first-page":"14510","DOI":"10.1021\/acsami.3c18596","article-title":"Nucleic acid and nanomaterial synergistic amplification enables dual targets of ultrasensitive fluorescence quantification to improve the efficacy of clinical tuberculosis diagnosis","volume":"16","author":"Shi","year":"2024","journal-title":"Acs. Appl. Mater. Inter."},{"issue":"1","key":"10.1016\/j.bios.2025.117566_bib31","article-title":"Multi-centric evaluation of truenat MTB and MTB-RIF Dx assays for diagnosis of extrapulmonary tuberculosis","volume":"14","author":"Singh","year":"2024","journal-title":"Sci. Rep."},{"issue":"8","key":"10.1016\/j.bios.2025.117566_bib32","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.1021\/ac400366b","article-title":"Selection of DNA aptamers against epithelial cell adhesion molecule for cancer cell imaging and circulating tumor cell capture","volume":"85","author":"Song","year":"2013","journal-title":"Anal. Chem."},{"issue":"6","key":"10.1016\/j.bios.2025.117566_bib33","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.jinf.2014.05.015","article-title":"CFP10 and ESAT6 aptamers as effective mycobacterial antigen diagnostic reagents","volume":"69","author":"Tang","year":"2014","journal-title":"J. Infect."},{"issue":"12","key":"10.1016\/j.bios.2025.117566_bib34","doi-asserted-by":"crossref","first-page":"14445","DOI":"10.1021\/acsami.3c17039","article-title":"Solid-phase extraction and enhanced amplification-free detection of pathogens integrated by multifunctional CRISPR-Cas12a","volume":"16","author":"Tian","year":"2024","journal-title":"Acs. Appl. Mater. Inter."},{"issue":"5","key":"10.1016\/j.bios.2025.117566_bib35","doi-asserted-by":"crossref","first-page":"449","DOI":"10.6026\/973206300200449","article-title":"Synthesis and molecular docking analysis of MBH adducts' derived amides as potential beta-lactamase inhibitors","volume":"20","author":"Ullah","year":"2024","journal-title":"Bioinformation"},{"issue":"8","key":"10.1016\/j.bios.2025.117566_bib36","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nchembio.609","article-title":"Aptamers for allosteric regulation","volume":"7","author":"Vinkenborg","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bios.2025.117566_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114282","article-title":"Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection","volume":"211","author":"Wei","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"2","key":"10.1016\/j.bios.2025.117566_bib38","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01368-19","article-title":"Cas12a\/Guide RNA-based platform for rapid and accurate identification of major mycobacterium species","volume":"58","author":"Xiao","year":"2020","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.bios.2025.117566_bib39","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.cell.2016.04.003","article-title":"Crystal structure of Cpf1 in complex with guide RNA and target DNA","volume":"165","author":"Yamano","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.bios.2025.117566_bib40","article-title":"A rapid, accurate, and low-cost method for detecting Mycobacterium tuberculosis and its drug-resistant genes in pulmonary tuberculosis: applications of MassARRAY DNA mass spectrometry","volume":"14","author":"Yang","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.bios.2025.117566_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.116980","article-title":"CRISPR-based nucleic acid diagnostics for pathogens","volume":"160","author":"Yang","year":"2023","journal-title":"Trac-Trend. Anal. Chem."},{"issue":"5","key":"10.1016\/j.bios.2025.117566_bib42","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.3892\/ijmm.2015.2131","article-title":"iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis","volume":"35","author":"Yang","year":"2015","journal-title":"Int. J. Mol. Med."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325004403?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325004403?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:44:56Z","timestamp":1777596296000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566325004403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":42,"alternative-id":["S0956566325004403"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2025.117566","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6\/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2025.117566","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117566"}}