{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:18:41Z","timestamp":1791263921376,"version":"4.1.0"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100014103","name":"Key Technology Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2024CXGC010918"],"award-info":[{"award-number":["2024CXGC010918"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014103","name":"Key Technology Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2024TSGC0318"],"award-info":[{"award-number":["2024TSGC0318"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023MC088"],"award-info":[{"award-number":["ZR2023MC088"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009620","name":"Science and Technology Department of Gansu Province","doi-asserted-by":"publisher","award":["22CX8NA023"],"award-info":[{"award-number":["22CX8NA023"]}],"id":[{"id":"10.13039\/501100009620","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372438"],"award-info":[{"award-number":["32372438"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872909"],"award-info":[{"award-number":["31872909"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772068"],"award-info":[{"award-number":["31772068"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.bios.2025.117593","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T11:02:05Z","timestamp":1747652525000},"page":"117593","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"special_numbering":"C","title":["\"Turn-on\" aptamer-immune lateral flow assays for the detection of small molecule targets based on CHA-assisted and CRISPR\/Cas12a mediated signal transduction and amplification"],"prefix":"10.1016","volume":"285","author":[{"given":"Haowei","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jicheng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangling","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5640-443X","authenticated-orcid":false,"given":"Yemin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2025.117593_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.nantod.2024.102403","article-title":"Integration of a new generation of immunochromatographic assays: Recent advances and future trends","volume":"57","author":"Bai","year":"2024","journal-title":"Nano Today"},{"key":"10.1016\/j.bios.2025.117593_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.162279","article-title":"Portable biosensor for on-site detection of kanamycin in water samples based on CRISPR-Cas12a and an off-the-shelf glucometer","volume":"872","author":"Chen","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.bios.2025.117593_bib3","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1007\/s00604-024-06552-5","article-title":"SERS biosensors based on catalytic hairpin self-assembly and hybridization chain reaction Cascade signal amplification strategies for ultrasensitive microRNA-21 detection","volume":"191","author":"Chen","year":"2024","journal-title":"Microchim. Acta"},{"key":"10.1016\/j.bios.2025.117593_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2019.126645","article-title":"A turn-on fluorescent chemosensor based on aggregation-induced emission for cyanide detection and its bioimaging applications","volume":"296","author":"Deng","year":"2019","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2025.117593_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342922","article-title":"Combination of Capture-SELEX and Post-SELEX for procymidone-specific aptamer selection and broad-specificity aptamer discovery, and development of aptamer-based lateral flow assay","volume":"1318","author":"Dong","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.bios.2025.117593_bib6","doi-asserted-by":"crossref","first-page":"28933","DOI":"10.1021\/acsami.3c03399","article-title":"Ultrasensitive detection of miRNA via CRISPR\/Cas12a coupled with strand displacement amplification reaction","volume":"15","author":"Feng","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.bios.2025.117593_bib7","doi-asserted-by":"crossref","first-page":"11099","DOI":"10.1021\/acs.nanolett.3c03448","article-title":"Nano-impact electrochemical biosensing based on a CRISPR-responsive DNA hydrogel","volume":"23","author":"Guo","year":"2023","journal-title":"Nano Lett."},{"key":"10.1016\/j.bios.2025.117593_bib8","doi-asserted-by":"crossref","first-page":"4193","DOI":"10.1021\/acs.jafc.2c08888","article-title":"An ultrasensitive colorimetric foodborne pathogenic detection method using a CRISPR\/Cas12a mediated strand displacement\/hybridization chain reaction","volume":"71","author":"Jiang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.bios.2025.117593_bib9","doi-asserted-by":"crossref","first-page":"14000","DOI":"10.1021\/acs.jafc.4c03601","article-title":"An RPA-assisted CRISPR\/Cas12a assay combining fluorescence and lateral flow strips for the rapid detection of enterotoxigenic bacillus Cereus","volume":"72","author":"Li","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.bios.2025.117593_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114725","article-title":"Accelerated CRISPR\/Cas12a-based small molecule detection using bivalent aptamer","volume":"217","author":"Li","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.117593_bib11","doi-asserted-by":"crossref","first-page":"13577","DOI":"10.1021\/acs.jafc.3c03619","article-title":"CRISPR\/cas system: The accelerator for the development of non-nucleic acid target detection in food safety","volume":"71","author":"Li","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.bios.2025.117593_bib12","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1021\/acssensors.2c01636","article-title":"A label-free photoelectrochemical biosensor based on CRISPR\/Cas12a system responsive deoxyribonucleic acid hydrogel and \u201cClick\u201d","volume":"7","author":"Liu","year":"2022","journal-title":"Chemistry. ACS Sens."},{"key":"10.1016\/j.bios.2025.117593_bib13","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3390\/ph15010090","article-title":"Aptamer-based lateral flow assays: Current trends in clinical diagnostic rapid tests","volume":"15","author":"Majdinasab","year":"2022","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.bios.2025.117593_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115188","article-title":"Fluorescent aptasensor for the ultrasensitive detection of antibiotic residue in food samples based on dumbbell DNA-Mediated signal amplification","volume":"228","author":"Shi","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.117593_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2022.132537","article-title":"Multiplexed lateral flow assay integrated with orthogonal CRISPR-cas system for SARS-CoV-2 detection","volume":"371","author":"Su","year":"2022","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2025.117593_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2023.103104","article-title":"An effective strategy of designing \u201cturn on\u201d mode-based lateral flow chromatography for rapid detection of aflatoxin B1","volume":"56","author":"Tang","year":"2023","journal-title":"Food Biosci."},{"key":"10.1016\/j.bios.2025.117593_bib17","doi-asserted-by":"crossref","first-page":"5174","DOI":"10.7150\/thno.56471","article-title":"Development of nucleic acid aptamer-based lateral flow assays: A robust platform for cost-effective point-of-care diagnosis","volume":"11","author":"Wang","year":"2021","journal-title":"Theranostics"},{"key":"10.1016\/j.bios.2025.117593_bib18","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.bios.2017.05.017","article-title":"Novel label-free and high-throughput microchip electrophoresis platform for multiplex antibiotic residues detection based on aptamer probes and target catalyzed hairpin assembly for signal amplification","volume":"97","author":"Wang","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.117593_bib19","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.talanta.2017.10.002","article-title":"A turn-on competitive immunochromatographic strips integrated with quantum dots and gold nano-stars for cadmium ion detection","volume":"178","author":"Xiao","year":"2018","journal-title":"Talanta"},{"key":"10.1016\/j.bios.2025.117593_bib20","doi-asserted-by":"crossref","first-page":"10664","DOI":"10.1021\/acs.analchem.3c01307","article-title":"Unlocking the full potential of Cas12a: Exploring the effects of substrate and reaction conditions on trans-cleavage activity","volume":"95","author":"Xu","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2025.117593_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2022.131545","article-title":"A comparative study of \u201cturn-off\u201d mode and \u201cturn-on\u201d mode lateral flow immunoassay for T-2 toxin detection","volume":"359","author":"Zhang","year":"2022","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2025.117593_bib22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.scib.2020.09.004","article-title":"A versatile biosensing platform coupling CRISPR\u2013Cas12a and aptamers for detection of diverse analytes","volume":"66","author":"Zhao","year":"2021","journal-title":"Science Bulletin"},{"key":"10.1016\/j.bios.2025.117593_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2024.136259","article-title":"Non-enzymatic aptazyme-driven autonomous DNA circuits coupled with three-junction CHA for amplified live-cell ATP imaging","volume":"418","author":"Zhu","year":"2024","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2025.117593_bib24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.snb.2015.11.089","article-title":"A \u201cturn-on\u201d fluorescence probe towards copper ions based on core-substitued naphthalene diimide","volume":"226","author":"Zong","year":"2016","journal-title":"Sensor. Actuator. B Chem."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325004671?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325004671?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T10:35:16Z","timestamp":1762338916000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566325004671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":24,"alternative-id":["S0956566325004671"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2025.117593","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"\"Turn-on\" aptamer-immune lateral flow assays for the detection of small molecule targets based on CHA-assisted and CRISPR\/Cas12a mediated signal transduction and amplification","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2025.117593","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117593"}}