{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:53:48Z","timestamp":1789181628671,"version":"build-2803163510"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42177212"],"award-info":[{"award-number":["42177212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42477491"],"award-info":[{"award-number":["42477491"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.bios.2025.118331","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T07:45:45Z","timestamp":1766389545000},"page":"118331","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Ultrasensitive profiling of circulating tumor cells via miRNA\/pH-activated nanoprobes coupled with microfluidic droplet encapsulation"],"prefix":"10.1016","volume":"296","author":[{"given":"Shixin","family":"Cai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongfeng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8616-3977","authenticated-orcid":false,"given":"Lele","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunpeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2906-7781","authenticated-orcid":false,"given":"Nandi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"35","key":"10.1016\/j.bios.2025.118331_bib1","doi-asserted-by":"crossref","first-page":"14222","DOI":"10.1021\/acs.analchem.4c02876","article-title":"An integrated inertial-magnetophoresis microfluidic chip online-coupled with ICP-MS for rapid separation and precise detection of circulating tumor cells","volume":"96","author":"Cai","year":"2024","journal-title":"Anal. Chem."},{"issue":"49","key":"10.1016\/j.bios.2025.118331_bib2","doi-asserted-by":"crossref","first-page":"18199","DOI":"10.1021\/acs.analchem.3c03843","article-title":"Nanosensor based on the dual-entropy-driven modulation strategy for intracellular detection of microRNA","volume":"95","author":"Cai","year":"2023","journal-title":"Anal. Chem."},{"issue":"4","key":"10.1016\/j.bios.2025.118331_bib3","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.31635\/ccschem.023.202303010","article-title":"Phenotype-directed DNA nanomachine for in situ analysis of stem cell-like subpopulation in breast cancer","volume":"6","author":"Cao","year":"2024","journal-title":"CCS Chem."},{"key":"10.1016\/j.bios.2025.118331_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114607","article-title":"A liquid-crystal aptasensing platform for label-free detection of a single circulating tumor cell","volume":"216","author":"Chang","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"32","key":"10.1016\/j.bios.2025.118331_bib5","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202201779","article-title":"2D MOF nanosensor\u2010integrated digital droplet microfluidic flow cytometry for in situ detection of multiple miRNAs in single CTC cells","volume":"18","author":"Chen","year":"2022","journal-title":"Small"},{"issue":"24","key":"10.1016\/j.bios.2025.118331_bib6","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202425252","article-title":"DNA framework-ensembled aptamers enhance fluid stability in circulating tumor cells capture for tumor treatment evaluation","volume":"64","author":"Chen","year":"2025","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.bios.2025.118331_bib7","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.matlet.2012.01.004","article-title":"Synthesis of ZnO nanoparticles by modified polyol method","volume":"73","author":"Chieng","year":"2012","journal-title":"Mater. Lett."},{"key":"10.1016\/j.bios.2025.118331_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2022.104237","article-title":"Circulating tumor cell isolation for cancer diagnosis and prognosis","volume":"83","author":"Deng","year":"2022","journal-title":"EBioMedicine"},{"issue":"4","key":"10.1016\/j.bios.2025.118331_bib9","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1039\/C6SC04633A","article-title":"A smart ZnO@polydopamine-nucleic acid nanosystem for ultrasensitive live cell mRNA imaging by the target-triggered intracellular self-assembly of active DNAzyme nanostructures","volume":"8","author":"He","year":"2017","journal-title":"Chem. Sci."},{"issue":"6","key":"10.1016\/j.bios.2025.118331_bib10","doi-asserted-by":"crossref","first-page":"5017","DOI":"10.1021\/acsnano.3c11147","article-title":"Single-cell liquid biopsy of lung cancer: ultra-simplified efficient enrichment of circulating tumor cells and hand-held fluorometer portable testing","volume":"18","author":"He","year":"2024","journal-title":"ACS Nano"},{"issue":"9","key":"10.1016\/j.bios.2025.118331_bib11","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1021\/acssensors.4c01357","article-title":"SERS-based droplet microfluidic platform for sensitive and high-throughput detection of cancer exosomes","volume":"9","author":"Ho","year":"2024","journal-title":"ACS Sens."},{"key":"10.1016\/j.bios.2025.118331_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116897","article-title":"Droplet-based digital PCR (ddPCR) and its applications","volume":"158","author":"Hou","year":"2023","journal-title":"Trends Anal. Chem."},{"issue":"14","key":"10.1016\/j.bios.2025.118331_bib13","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202413978","article-title":"Linking metastatic behavior and metabolic heterogeneity of circulating tumor cells at single\u2010cell level using an integrative microfluidic system","volume":"12","author":"Hou","year":"2025","journal-title":"Adv. Sci."},{"issue":"6","key":"10.1016\/j.bios.2025.118331_bib15","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1021\/am400253d","article-title":"Enzyme-mimetic catalyst-modified nanoporous SiO2\u2013cellulose hybrid composites with high specific surface area for rapid H2O2 detection","volume":"5","author":"Jiang","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"1","key":"10.1016\/j.bios.2025.118331_bib16","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s40364-022-00403-2","article-title":"Detection of circulating tumor cells: opportunities and challenges","volume":"10","author":"Ju","year":"2022","journal-title":"Biomark. Res."},{"issue":"1","key":"10.1016\/j.bios.2025.118331_bib17","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/jhg.2016.89","article-title":"Recent trends in microRNA research into breast cancer with particular focus on the associations between microRNAs and intrinsic subtypes","volume":"62","author":"Kurozumi","year":"2016","journal-title":"J. Hum. Genet."},{"issue":"5","key":"10.1016\/j.bios.2025.118331_bib18","article-title":"A phenotype-independent \u201clabel-capture-release\u201d process for isolating viable circulating tumor cells in real-time drug susceptibility testing","volume":"6","author":"Lao","year":"2025","journal-title":"Innovation"},{"issue":"19","key":"10.1016\/j.bios.2025.118331_bib19","doi-asserted-by":"crossref","first-page":"18117","DOI":"10.1021\/acsnano.4c15056","article-title":"A digital score based on circulating-tumor-cells-derived mRNA quantification and machine learning for early colorectal cancer detection","volume":"19","author":"Li","year":"2025","journal-title":"ACS Nano"},{"key":"10.1016\/j.bios.2025.118331_bib20","article-title":"Dual trace detection of hazardous substances by aptamer-functionalized gold nanofilms based on surface-enhanced Raman spectroscopy","volume":"55","author":"Li","year":"2024","journal-title":"Surf. Interfaces"},{"issue":"6","key":"10.1016\/j.bios.2025.118331_bib21","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1038\/s41592-024-02586-y","article-title":"UDA-seq: universal droplet microfluidics-based combinatorial indexing for massive-scale multimodal single-cell sequencing","volume":"22","author":"Li","year":"2025","journal-title":"Nat. Method"},{"issue":"7","key":"10.1016\/j.bios.2025.118331_bib22","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1373\/clinchem.2015.253716","article-title":"Direct comparison of metastasis-related miRNAs expression levels in circulating tumor cells, corresponding plasma, and primary tumors of breast cancer patients","volume":"62","author":"Lianidou","year":"2016","journal-title":"Clin. Chem."},{"issue":"9","key":"10.1016\/j.bios.2025.118331_bib23","doi-asserted-by":"crossref","first-page":"4263","DOI":"10.1021\/acs.biomac.3c00557","article-title":"pH-responsive zinc ion regulating immunomodulatory nanoparticles for effective cancer immunotherapy","volume":"24","author":"Lim","year":"2023","journal-title":"Biomacromolecules"},{"issue":"15","key":"10.1016\/j.bios.2025.118331_bib24","article-title":"miRNA21 as a specific marker for the detection of circulating tumor cell","volume":"33","author":"Ordonez","year":"2015","journal-title":"J. Clin. Oncol."},{"issue":"3","key":"10.1016\/j.bios.2025.118331_bib25","doi-asserted-by":"crossref","DOI":"10.1002\/admt.202400477","article-title":"Dual nozzle\u2010assisted deterministic encapsulation of triple particles for screening NK\u2010Cell cytotoxicity against circulating tumor cell clusters","volume":"10","author":"Park","year":"2024","journal-title":"Adv. Mater. Technol."},{"issue":"5","key":"10.1016\/j.bios.2025.118331_bib27","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.1016\/j.cclet.2021.08.128","article-title":"Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics","volume":"33","author":"Qiu","year":"2022","journal-title":"Chin. Chem. Lett."},{"issue":"2","key":"10.1016\/j.bios.2025.118331_bib28","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1021\/acs.analchem.1c04194","article-title":"Single-cell digital microfluidic mass spectrometry platform for efficient and multiplex genotyping of circulating tumor cells","volume":"94","author":"Ruan","year":"2021","journal-title":"Anal. Chem."},{"issue":"45","key":"10.1016\/j.bios.2025.118331_bib29","doi-asserted-by":"crossref","first-page":"16489","DOI":"10.1021\/acs.analchem.3c02007","article-title":"Nucleic acid amplification-free digital detection method for SARS-CoV-2 RNA based on droplet microfluidics and CRISPR-Cas13a","volume":"95","author":"Shan","year":"2023","journal-title":"Anal. Chem."},{"issue":"4","key":"10.1016\/j.bios.2025.118331_bib30","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1021\/acs.nanolett.2c04658","article-title":"Bioorthogonal disassembly of hierarchical DNAzyme nanogel for high-performance intracellular microRNA imaging","volume":"23","author":"Shang","year":"2023","journal-title":"Nano Lett."},{"key":"10.1016\/j.bios.2025.118331_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114025","article-title":"Combination of microfluidic chips and biosensing for the enrichment of circulating tumor cells","volume":"202","author":"Shi","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"3","key":"10.1016\/j.bios.2025.118331_bib32","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4161\/cbt.23296","article-title":"Role of microRNAs in breast cancer","volume":"14","author":"Singh","year":"2013","journal-title":"Cancer Biol. Ther."},{"issue":"27","key":"10.1016\/j.bios.2025.118331_bib33","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202402379","article-title":"Precise capture and dynamic release of circulating liver cancer cells with dual\u2010histidine\u2010based cell imprinted hydrogels","volume":"36","author":"Sun","year":"2024","journal-title":"Adv. Mater."},{"key":"10.1016\/j.bios.2025.118331_bib34","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.snb.2019.05.002","article-title":"pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy for sensitive detection and live cell imaging of multiple microRNAs","volume":"293","author":"Wang","year":"2019","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.bios.2025.118331_bib35","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.aca.2019.02.049","article-title":"MnO2 nanosheet-mediated ratiometric fluorescence biosensor for MicroRNA detection and imaging in living cells","volume":"1063","author":"Wang","year":"2019","journal-title":"Anal. Chim. Acta"},{"issue":"13","key":"10.1016\/j.bios.2025.118331_bib36","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202411459","article-title":"Data\u2010driven theoretical modeling of centrifugal step emulsification and its application in comprehensive multiscale analysis","volume":"12","author":"Wang","year":"2025","journal-title":"Adv. Sci."},{"key":"10.1016\/j.bios.2025.118331_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.117011","article-title":"Organic photoelectrochemical transistor biosensor based on BiVO4-ZnIn2S4 material for efficient and sensitive detection of MCF-7 cells","volume":"271","author":"Wang","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.118331_bib38","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.pbiomolbio.2022.12.005","article-title":"Clinical significance of circulating tumor cell (CTC)-specific microRNA (miRNA) in breast cancer","volume":"177","author":"Wei","year":"2023","journal-title":"Prog. Biophys. Mol. Biol."},{"issue":"1","key":"10.1016\/j.bios.2025.118331_bib39","doi-asserted-by":"crossref","first-page":"9146","DOI":"10.1038\/s41467-024-53227-z","article-title":"Overloading and unpacking (OAK) - droplet-based combinatorial indexing for ultra-high throughput single-cell multiomic profiling","volume":"15","author":"Wu","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bios.2025.118331_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117358","article-title":"Ratiometric electrochemiluminescence biosensor based on red blood cell membrane as an efficient antifouling interface for ultrasensitive analysis of circulating tumor cells in human serum","volume":"278","author":"Wu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.118331_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116984","article-title":"Antigen-independent single-cell circulating tumor cell detection using deep-learning-assisted biolasers","volume":"271","author":"Wu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2025.118331_bib42","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1159\/000481648","article-title":"MicroRNA-21 (Mir-21) promotes cell growth and invasion by repressing tumor suppressor PTEN in colorectal cancer","volume":"43","author":"Wu","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"issue":"18","key":"10.1016\/j.bios.2025.118331_bib43","doi-asserted-by":"crossref","first-page":"7131","DOI":"10.1021\/acs.jafc.3c00508","article-title":"Development of a fluorescent biosensor based on DNAzyme for tracing the release of zinc in maize leaves","volume":"71","author":"Yan","year":"2023","journal-title":"J. Agric. Food Chem."},{"issue":"1","key":"10.1016\/j.bios.2025.118331_bib44","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1186\/s12943-019-1043-x","article-title":"Liquid biopsy in hepatocellular carcinoma: circulating tumor cells and circulating tumor DNA","volume":"18","author":"Ye","year":"2019","journal-title":"Mol. Cancer"},{"issue":"10","key":"10.1016\/j.bios.2025.118331_bib45","doi-asserted-by":"crossref","first-page":"7073","DOI":"10.1039\/D5TA00494B","article-title":"Structured droplets dominated by interfacial self-assembly of topology-tunable Janus particles towards macroscopic materials","volume":"13","author":"Zhai","year":"2025","journal-title":"J. Mater. Chem. A"},{"key":"10.1016\/j.bios.2025.118331_bib46","article-title":"Ingenious integration of synthetic biology and droplet microfluidics","volume":"520","author":"Zhang","year":"2025","journal-title":"Chem. Eng. J."},{"issue":"7","key":"10.1016\/j.bios.2025.118331_bib47","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1021\/acs.nanolett.3c04828","article-title":"Controllable assembly of a quantum dot-based aptasensor guided by CRISPR\/Cas12a for direct measurement of circulating tumor cells in human blood","volume":"24","author":"Zhang","year":"2024","journal-title":"Nano Lett."},{"key":"10.1016\/j.bios.2025.118331_bib48","article-title":"A detachable magnetic nanodevice for the efficient capture and subtype identification of circulating tumor cells","volume":"34","author":"Zhang","year":"2024","journal-title":"Adv. Funct. Mater."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325012084?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566325012084?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T15:40:37Z","timestamp":1773934837000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566325012084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":46,"alternative-id":["S0956566325012084"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2025.118331","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ultrasensitive profiling of circulating tumor cells via miRNA\/pH-activated nanoprobes coupled with microfluidic droplet encapsulation","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2025.118331","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"118331"}}