{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:47:19Z","timestamp":1789660039042,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002865","name":"Chongqing Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["2026KWJK1007"],"award-info":[{"award-number":["2026KWJK1007"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing Municipality","doi-asserted-by":"publisher","award":["CSTB2023NSCQ-MSX0147"],"award-info":[{"award-number":["CSTB2023NSCQ-MSX0147"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.bios.2026.118428","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:14:54Z","timestamp":1769040894000},"page":"118428","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["A DNAzyme-CRISPR cascade strategy for preamplification-free detection of Mycobacterium tuberculosis"],"prefix":"10.1016","volume":"298","author":[{"given":"Ying","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoming","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxin","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulei","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-8700","authenticated-orcid":false,"given":"Hui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2026.118428_bib1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1021\/acscentsci.0c01345","article-title":"Chemiluminescent protease probe for rapid, sensitive, and inexpensive detection of live Mycobacterium tuberculosis","volume":"7","author":"Babin","year":"2021","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.bios.2026.118428_bib2","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adx2067","article-title":"A streamlined CRISPR-based test for tuberculosis detection directly from sputum","author":"Bell","year":"2025","journal-title":"Sci. Adv."},{"key":"10.1016\/j.bios.2026.118428_bib3","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/S0140-6736(11)60438-8","article-title":"Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB\/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study","volume":"377","author":"Boehme","year":"2011","journal-title":"Lancet"},{"key":"10.1016\/j.bios.2026.118428_bib4","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1038\/s41467-025-59219-x","article-title":"Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-Cas\u03a6 tool","volume":"16","author":"Chen","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bios.2026.118428_bib5","article-title":"Making target sites in large structured RNAs accessible to RNA-cleaving DNAzymes through hybridization with synthetic DNA oligonucleotides","volume":"52","author":"Connor","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bios.2026.118428_bib6","doi-asserted-by":"crossref","first-page":"20204","DOI":"10.1021\/acs.analchem.4c04180","article-title":"Point-of-Care diagnosis of tuberculosis using a portable nucleic acid Test with distance-based readout","volume":"96","author":"Dong","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2026.118428_bib7","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S1473-3099(17)30691-6","article-title":"Xpert MTB\/RIF Ultra for detection of Mycobacterium tuberculosis and rifampicin resistance: a prospective multicentre diagnostic accuracy study","volume":"18","author":"Dorman","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.bios.2026.118428_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116740","article-title":"An extraction-free one-pot assay for rapid detection of Klebsiella pneumoniae by combining RPA and CRISPR\/Cas12a","volume":"267","author":"Fu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2026.118428_bib9","series-title":"Global Tuberculosis Report 2024","year":"2024"},{"key":"10.1016\/j.bios.2026.118428_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.abj4124","article-title":"Point-of-care diagnostic tests for tuberculosis disease","volume":"14","author":"Hong","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.bios.2026.118428_bib11","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1021\/acsnano.3c09506","article-title":"DNAzyme-Based dissipative DNA strand displacement for constructing temporal logic gates","volume":"18","author":"Hu","year":"2024","journal-title":"ACS Nano"},{"key":"10.1016\/j.bios.2026.118428_bib13","doi-asserted-by":"crossref","first-page":"11148","DOI":"10.1093\/nar\/gkae766","article-title":"Synergistic effect of split DNA activators of Cas12a with exon-unwinding and induced targeting effect","volume":"52","author":"Huang","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bios.2026.118428_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(25)00214-2","article-title":"The health impact of identifying a person with tuberculosis through systematic screening","author":"Kendall","year":"2025","journal-title":"Lancet Infect. Dis. S1473309925002142"},{"key":"10.1016\/j.bios.2026.118428_bib15","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf596","article-title":"PAM-free hairpin target binding activates trans -cleavage activity of Cas12a","volume":"53","author":"Li","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bios.2026.118428_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117566","article-title":"Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6\/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)","volume":"284","author":"Liu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2026.118428_bib17","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202001300","article-title":"MeCas12a, a highly sensitive and specific System for COVID\u201019 detection","volume":"7","author":"Ma","year":"2020","journal-title":"Adv. Sci."},{"key":"10.1016\/j.bios.2026.118428_bib18","doi-asserted-by":"crossref","DOI":"10.1038\/s41551-025-01441-5","article-title":"Self-powered rapid antigen-specific T-cell response assay for Mycobacterium tuberculosis infections","author":"Ning","year":"2025","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.bios.2026.118428_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2025.168050","article-title":"Functional alliance of DNAzymes and CRISPR\/Cas12a for non-nucleic acid target detection","volume":"522","author":"Ruan","year":"2025","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.bios.2026.118428_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2025.105611","article-title":"Speeding up drug susceptibility testing in Mycobacterium tuberculosis using RNA biomarkers","volume":"113","author":"Sury","year":"2025","journal-title":"EBioMedicine"},{"key":"10.1016\/j.bios.2026.118428_bib21","doi-asserted-by":"crossref","first-page":"13950","DOI":"10.1021\/acsnano.4c04147","article-title":"Autonomous feedback-driven engineered DNAzyme-Coated trojan horse-like nanocapsules for On-Demand CRISPR\/Cas9 delivery","volume":"18","author":"Tang","year":"2024","journal-title":"ACS Nano"},{"key":"10.1016\/j.bios.2026.118428_bib22","doi-asserted-by":"crossref","DOI":"10.1002\/smmd.70007","article-title":"The Recent development in the diagnosis of Mycobacterium tuberculosis","volume":"4","author":"Temur","year":"2025","journal-title":"Smart Medicine"},{"key":"10.1016\/j.bios.2026.118428_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117720","article-title":"CRISPR\/Cas12a-mediated DNAzyme\/split-aptamer cascade for label-free detection of site-specific DNA methylation","volume":"287","author":"Tian","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2026.118428_bib24","doi-asserted-by":"crossref","DOI":"10.1126\/science.add8643","article-title":"CRISPR technology: a decade of genome editing is only the beginning","volume":"379","author":"Wang","year":"2023","journal-title":"Science"},{"key":"10.1016\/j.bios.2026.118428_bib25","doi-asserted-by":"crossref","first-page":"17926","DOI":"10.1021\/jacs.3c05413","article-title":"Site-Specific bioorthogonal activation of DNAzymes for On-Demand gene therapy","volume":"145","author":"Wang","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bios.2026.118428_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2020.103007","article-title":"A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity","volume":"60","author":"Wang","year":"2020","journal-title":"EBioMedicine"},{"key":"10.1016\/j.bios.2026.118428_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinf.2025.106517","article-title":"Tongue swab-based molecular diagnostics for pulmonary tuberculosis and drug resistance in adults: a prospective multicenter diagnostic accuracy study","volume":"91","author":"Wang","year":"2025","journal-title":"J. Infect."},{"key":"10.1016\/j.bios.2026.118428_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.168708","article-title":"Dual-functional DNAzyme powered CRISPR-Cas12a sensor for ultrasensitive and high-throughput detection of Pb2+ in freshwater","volume":"911","author":"Wen","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.bios.2026.118428_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.154542","article-title":"Three-way junction-mediated three-letter coded SDA cascade CRISPR\/Cas12a system for circRNA detection","volume":"497","author":"Wu","year":"2024","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.bios.2026.118428_bib32","doi-asserted-by":"crossref","first-page":"10159","DOI":"10.1021\/acs.analchem.2c01461","article-title":"Kinetics accelerated CRISPR-Cas12a enabling live-cell monitoring of Mn2+ homeostasis","volume":"94","author":"Xie","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2026.118428_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117539","article-title":"An integrated wearable fluorescence sensor for E. coli detection in catheter bags","volume":"283","author":"Xu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2026.118428_bib34","article-title":"NIR-II aggregation-induced emission nanoparticles camouflaged with preactivated macrophage membranes for phototheranostics of pulmonary tuberculosis","author":"Yan","year":"2025","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.bios.2026.118428_bib35","article-title":"Subcellular compartment\u2010specific amplified imaging of metal ions via ribosomal RNA\u2010Regulated DNAzyme sensors","author":"Yi","year":"2024","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.bios.2026.118428_bib36","doi-asserted-by":"crossref","first-page":"11456","DOI":"10.1039\/D5SC01759A","article-title":"Regulating CRISPR\/Cas12a trans-cleavage on the hairpin DNA\u2013MB nanointerface for enhanced multiplexed sensing application","volume":"16","author":"Zhao","year":"2025","journal-title":"Chem. Sci."},{"key":"10.1016\/j.bios.2026.118428_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.124582","article-title":"CRISPR\/Cas13a-triggered Cas12a biosensing method for ultrasensitive and specific miRNA detection","volume":"260","author":"Zhao","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.bios.2026.118428_bib38","doi-asserted-by":"crossref","first-page":"11884","DOI":"10.1093\/nar\/gkae804","article-title":"FRAME: flap endonuclease 1-engineered PAM module for precise and sensitive modulation of CRISPR\/Cas12a trans -cleavage activity","volume":"52","author":"Zuo","year":"2024","journal-title":"Nucleic Acids Res."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566326000606?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566326000606?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:37:47Z","timestamp":1778564267000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566326000606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":35,"alternative-id":["S0956566326000606"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2026.118428","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A DNAzyme-CRISPR cascade strategy for preamplification-free detection of Mycobacterium tuberculosis","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2026.118428","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"118428"}}