{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:14:56Z","timestamp":1792516496204,"version":"4.5.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82504552"],"award-info":[{"award-number":["82504552"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473636"],"award-info":[{"award-number":["82473636"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373628"],"award-info":[{"award-number":["82373628"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018544","name":"Ningbo Municipal Science and Technology Innovative Research Team","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018544","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biosensors and Bioelectronics"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.bios.2026.118953","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T16:31:58Z","timestamp":1782145918000},"page":"118953","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Multiple DNA cycle amplification-assisted one-pot isothermal Cas12a for ultrasensitive nucleic acid detection"],"prefix":"10.1016","volume":"311","author":[{"given":"Xiaoxi","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3569-2732","authenticated-orcid":false,"given":"Chao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bios.2026.118953_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115940","article-title":"CeO(2) nanozyme mediated RPA\/CRISPR-Cas12a dual-mode biosensor for detection of invA gene in Salmonella","volume":"247","author":"Arshad","year":"2024","journal-title":"Biosens. Bioelectron."},{"issue":"7","key":"10.1016\/j.bios.2026.118953_bib2","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR-Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bios.2026.118953_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114152","article-title":"An RNA-based catalytic hairpin assembly circuit coupled with CRISPR-Cas12a for one-step detection of microRNAs","volume":"207","author":"Chen","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bios.2026.118953_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112642","article-title":"Contamination-free visual detection of SARS-CoV-2 with CRISPR\/Cas12a: a promising method in the point-of-care detection","volume":"169","author":"Chen","year":"2020","journal-title":"Biosens. Bioelectron."},{"issue":"3","key":"10.1016\/j.bios.2026.118953_bib5","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Euro Surveill."},{"issue":"43","key":"10.1016\/j.bios.2026.118953_bib6","doi-asserted-by":"crossref","first-page":"15275","DOI":"10.1073\/pnas.0407024101","article-title":"Triggered amplification by hybridization chain reaction","volume":"101","author":"Dirks","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"10","key":"10.1016\/j.bios.2026.118953_bib7","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1073\/pnas.92.10.4641","article-title":"Rolling replication of short DNA circles","volume":"92","author":"Fire","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"26","key":"10.1016\/j.bios.2026.118953_bib8","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2202034119","article-title":"Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics","volume":"119","author":"Hu","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bios.2026.118953_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114382","article-title":"A programmable and sensitive CRISPR\/Cas12a-based MicroRNA detection platform combined with hybridization chain reaction","volume":"211","author":"Jia","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"7","key":"10.1016\/j.bios.2026.118953_bib10","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.bios.2026.118953_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114428","article-title":"Enhanced chemiluminescence imaging sensor for ultrasensitive detection of nucleic acids based on HCR-CRISPR\/Cas12a","volume":"212","author":"Ke","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"4","key":"10.1016\/j.bios.2026.118953_bib12","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/s41564-022-01089-w","article-title":"Metagenomics-enabled microbial surveillance","volume":"7","author":"Ko","year":"2022","journal-title":"Nat. Microbiol."},{"issue":"1","key":"10.1016\/j.bios.2026.118953_bib13","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1038\/s41467-022-29202-x","article-title":"Guide RNAs containing universal bases enable Cas9\/Cas12a recognition of polymorphic sequences","volume":"13","author":"Krysler","year":"2022","journal-title":"Nat. Commun."},{"issue":"6","key":"10.1016\/j.bios.2026.118953_bib14","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1093\/nar\/gkp026","article-title":"Circular dichroism and conformational polymorphism of DNA","volume":"37","author":"Kypr","year":"2009","journal-title":"Nucleic Acids Res."},{"issue":"7","key":"10.1016\/j.bios.2026.118953_bib15","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.tibtech.2018.12.005","article-title":"CRISPR\/cas systems towards next-generation biosensing","volume":"37","author":"Li","year":"2019","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.bios.2026.118953_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117221","article-title":"Development of a single-tube RPA\/CRISPR-cas12a detection platform for monkeypox virus","volume":"278","author":"Liu","year":"2025","journal-title":"Biosens. Bioelectron."},{"issue":"Pt 2","key":"10.1016\/j.bios.2026.118953_bib17","article-title":"Integrating CRISPR-Cas12a and rolling circle-amplified G-quadruplex for naked-eye fluorescent \"off-on\" detection of citrus alternaria","volume":"262","author":"Ma","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"12","key":"10.1016\/j.bios.2026.118953_bib18","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.bios.2026.118953_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.lanmic.2025.101079","article-title":"Wastewater surveillance for early warning of COVID-19 outbreaks in long-term care facilities","volume":"6","author":"Pan","year":"2025","journal-title":"Lancet Microbe"},{"issue":"9","key":"10.1016\/j.bios.2026.118953_bib20","doi-asserted-by":"crossref","first-page":"e290","DOI":"10.1016\/S1473-3099(21)00048-7","article-title":"Scaling up COVID-19 rapid antigen tests: promises and challenges","volume":"21","author":"Peeling","year":"2021","journal-title":"Lancet Infect. Dis."},{"issue":"1","key":"10.1016\/j.bios.2026.118953_bib21","doi-asserted-by":"crossref","first-page":"5125","DOI":"10.1038\/s41467-025-60490-1","article-title":"Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring","volume":"16","author":"Perez-Zabaleta","year":"2025","journal-title":"Nat. Commun."},{"issue":"7","key":"10.1016\/j.bios.2026.118953_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"issue":"5805","key":"10.1016\/j.bios.2026.118953_bib23","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1126\/science.1132493","article-title":"Enzyme-free nucleic acid logic circuits","volume":"314","author":"Seelig","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.bios.2026.118953_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.118141","article-title":"Ultra-fast one-pot isothermal detection of respiratory virus: ADNA-initiated CRISPR\/Cas12a-mediated RCA cycle","volume":"293","author":"Shan","year":"2026","journal-title":"Biosens. Bioelectron."},{"issue":"3","key":"10.1016\/j.bios.2026.118953_bib25","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/s41579-020-00461-z","article-title":"Considerations for diagnostic COVID-19 tests","volume":"19","author":"Vandenberg","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"issue":"12","key":"10.1016\/j.bios.2026.118953_bib26","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1038\/s41551-023-01033-1","article-title":"A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs","volume":"7","author":"Yan","year":"2023","journal-title":"Nat. Biomed. Eng."},{"issue":"39","key":"10.1016\/j.bios.2026.118953_bib27","doi-asserted-by":"crossref","first-page":"26657","DOI":"10.1021\/jacs.4c02354","article-title":"RNA-activated CRISPR\/Cas12a nanorobots operating in living cells","volume":"146","author":"Yuan","year":"2024","journal-title":"J. Am. Chem. Soc."},{"issue":"3","key":"10.1016\/j.bios.2026.118953_bib28","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.cell.2015.09.038","article-title":"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-cas system","volume":"163","author":"Zetsche","year":"2015","journal-title":"Cell"},{"issue":"34","key":"10.1016\/j.bios.2026.118953_bib29","first-page":"14028","article-title":"Electrochemical-fluorescent bimodal biosensor based on dual CRISPR-Cas12a multiple Cascade amplification for ctDNA detection","volume":"96","author":"Zhang","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bios.2026.118953_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.118008","article-title":"CRISPR-integrated nanoconfined interparticle catalytic hairpin assembly for enhanced dual-mode SARS-CoV-2 detection in wastewater","volume":"290","author":"Zheng","year":"2025","journal-title":"Biosens. Bioelectron."},{"issue":"13","key":"10.1016\/j.bios.2026.118953_bib31","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","article-title":"Mfold web server for nucleic acid folding and hybridization prediction","volume":"31","author":"Zuker","year":"2003","journal-title":"Nucleic Acids Res."}],"container-title":["Biosensors and Bioelectronics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566326005853?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0956566326005853?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:06:34Z","timestamp":1786709194000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0956566326005853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":31,"alternative-id":["S0956566326005853"],"URL":"https:\/\/doi.org\/10.1016\/j.bios.2026.118953","relation":{},"ISSN":["0956-5663"],"issn-type":[{"value":"0956-5663","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiple DNA cycle amplification-assisted one-pot isothermal Cas12a for ultrasensitive nucleic acid detection","name":"articletitle","label":"Article Title"},{"value":"Biosensors and Bioelectronics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bios.2026.118953","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"118953"}}