{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:51:00Z","timestamp":1792515060651,"version":"4.5.0"},"reference-count":176,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100006377","name":"Purdue University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Advances"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.biotechadv.2025.108764","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T22:00:58Z","timestamp":1763762458000},"page":"108764","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["From dye exclusion to high-throughput screening: A review of cell viability assays and their applications"],"prefix":"10.1016","volume":"87","author":[{"given":"Kumar Vishven","family":"Naveen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akanksha","family":"Tyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia Mohammed Hamid","family":"Ibrahium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer E.A.W.","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raluca","family":"Ostafe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biotechadv.2025.108764_bb0005","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.2174\/1389201017666160808160513","article-title":"Cell proliferation and cytotoxicity assays","volume":"17","author":"Adan","year":"2016","journal-title":"Curr. Pharm. Biotechnol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0010","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.xphs.2023.08.026","article-title":"Carfilzomib-loaded ternary polypeptide nanoparticles stabilized by Polycationic complexation","volume":"113","author":"Agbana","year":"2024","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0015","doi-asserted-by":"crossref","first-page":"770","DOI":"10.3390\/brainsci14080770","article-title":"Real-time analysis of neuronal cell cultures for CNS drug discovery","volume":"14","author":"Akere","year":"2024","journal-title":"Brain Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0020","first-page":"1505","article-title":"A simplified colorimetric method for rapid detection of cell viability and toxicity in adherent cell culture systems","volume":"23","author":"Alamoudi","year":"2018","journal-title":"J. BUON"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13287-019-1296-8","article-title":"Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity","volume":"10","author":"Andrzejewska","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0030","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/BF01784045","article-title":"Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake","volume":"70","author":"Antal","year":"1995","journal-title":"Ann. Hematol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0035","doi-asserted-by":"crossref","first-page":"112","DOI":"10.4103\/2228-7477.181040","article-title":"Multiple MTS assay as the alternative method to determine survival fraction of the irradiated HT-29 colon cancer cells","volume":"6","author":"Arab-Bafrani","year":"2016","journal-title":"J. Med. Signals Sens."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0040","first-page":"64","article-title":"In vitro cytotoxicity and cell viability assays: principles, advantages, and disadvantages","volume":"2","author":"Aslant\u00fcrk","year":"2018","journal-title":"Genotoxicity-A predictable risk to our actual world"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11033-025-10551-x","article-title":"Inhibitory effects of tetraselmis algae extract on cervical cancer cell proliferation: a molecular and cellular approach","volume":"52","author":"Asoudeh-Fard","year":"2025","journal-title":"Mol. Biol. Rep."},{"issue":"12","key":"10.1016\/j.biotechadv.2025.108764_bb0050","doi-asserted-by":"crossref","first-page":"5911","DOI":"10.3390\/ijms26125911","article-title":"Spectral flow cytometry: the current state and future of the technology","volume":"26","author":"Astakhova","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0262119","article-title":"Quantitative, traceable determination of cell viability using absorbance microscopy","volume":"17","author":"Babakhanova","year":"2022","journal-title":"PLoS ONE"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0060","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0005-2728(03)00110-5","article-title":"Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F0 during ATP synthesis","volume":"1606","author":"Baracca","year":"2003","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0065","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1369\/0022155413520404","article-title":"Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting","volume":"62","author":"Barteneva","year":"2014","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0070","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.cellbi.2006.06.016","article-title":"Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V\/propidium iodide versus acridin orange\/ethidium bromide","volume":"30","author":"Baski\u0107","year":"2006","journal-title":"Cell Biol. Int."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0075","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1387-2656(05)11004-7","article-title":"Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction","volume":"11","author":"Berridge","year":"2005","journal-title":"Biotechnol. Annu. Rev."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0080","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/BF01666038","article-title":"A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD\/NR-90)","volume":"9","author":"Borenfreund","year":"1985","journal-title":"J. Tissue Cult. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0887-2333(88)90030-6","article-title":"Comparisons of two in vitro cytotoxicity assays\u2014the neutral red (NR) and tetrazolium MTT tests","volume":"2","author":"Borenfreund","year":"1988","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0090","doi-asserted-by":"crossref","DOI":"10.1186\/s13014-020-01697-y","article-title":"The clonogenic assay: robustness of plating efficiency-based analysis is strongly compromised by cellular cooperation","volume":"15","author":"Brix","year":"2020","journal-title":"Radiat. Oncol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0095","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.drup.2010.09.001","article-title":"Apoptosis assays for quantifying the bioactivity of anticancer drug products","volume":"13","author":"Brunelle","year":"2010","journal-title":"Drug Resist. Updat."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0100","unstructured":"Cell Viability Assays Market Size & Share Report, 2024 \u2013 2032 2025. [WWW Document] URL https:\/\/www.gminsights.com\/industry-analysis\/cell-viability-assays-market (accessed 10.13.25)."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0105","first-page":"1","article-title":"In vitro immunotoxic evaluation of herbicides in RAW 264.7 cells","volume":"Part A","author":"Cestonaro","year":"2025","journal-title":"J. Toxicol. Environ. Health"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0110","doi-asserted-by":"crossref","first-page":"3269","DOI":"10.1021\/pr300018c","article-title":"Changes in mitochondrial proteome of renal tubular cells induced by calcium oxalate monohydrate crystal adhesion and internalization are related to mitochondrial dysfunction","volume":"11","author":"Chaiyarit","year":"2012","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0115","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/1097-4644(20010101)80:1<133::AID-JCB120>3.0.CO;2-T","article-title":"Vitamin a as an enzyme that catalyzes the reduction of MTT to formazan by vitamin C","volume":"80","author":"Chakrabarti","year":"2001","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0120","series-title":"Detection of Necrosis by Release of Lactate Dehydrogenase Activity. Immune Homeostasis: Methods and Protocols","first-page":"65","author":"Chan","year":"2013"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0125","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0227950","article-title":"Observation and quantification of the morphological effect of trypan blue rupturing dead or dying cells","volume":"15","author":"Chan","year":"2020","journal-title":"PLoS ONE"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0130","series-title":"The Respiratory Chain and Oxidative Phosphorylation","author":"Chance","year":"1956"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0135","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0887-2333(97)00107-0","article-title":"Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells","volume":"12","author":"Chiba","year":"1998","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0140","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s12935-025-03657-6","article-title":"Aniline TFPA enhances camptothecin-induced anti-NSCLC by modulating oxidative stress and impairing autophagy","volume":"25","author":"Chou","year":"2025","journal-title":"Cancer Cell Int."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0145","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ymeth.2013.03.020","article-title":"Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release","volume":"61","author":"Christensen","year":"2013","journal-title":"Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0150","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1038\/s41596-023-00840-9","article-title":"Optimizing the cell painting assay for image-based profiling","volume":"18","author":"Cimini","year":"2023","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0155","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/BF00256695","article-title":"Rapid chemosensitivity testing of human lung tumor cells using the MTT assay","volume":"17","author":"Cole","year":"1986","journal-title":"Cancer Chemother. Pharmacol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0160","doi-asserted-by":"crossref","first-page":"452","DOI":"10.4161\/cam.28348","article-title":"Live-cell imaging: the cell's perspective","volume":"8","author":"Cole","year":"2014","journal-title":"Cell Adhes. Migr."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0165","doi-asserted-by":"crossref","DOI":"10.1063\/5.0061373","article-title":"Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro","volume":"15","author":"Collins","year":"2021","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0170","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-97894-0","article-title":"Application of LDH assay for therapeutic efficacy evaluation of ex vivo tumor models","volume":"11","author":"Cox","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0175","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/gyno.1994.1187","article-title":"Cytotoxic drug sensitivity testing of tumor cells from patients with ovarian carcinoma using the fluorometric microculture cytotoxicity assay (FMCA)","volume":"54","author":"Csoka","year":"1994","journal-title":"Gynecol. Oncol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0180","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.toxlet.2018.12.004","article-title":"How reliable are in vitro IC50 values? Values vary with cytotoxicity assays in human glioblastoma cells","volume":"302","author":"Damiani","year":"2019","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0185","first-page":"799","article-title":"Interactions of rhodamine 123 with living cells studied by flow cytometry","volume":"42","author":"Darzynkiewicz","year":"1982","journal-title":"Cancer Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0190","article-title":"Leveraging 3D cell culture and AI technologies for next-generation drug discovery. Cell","volume":"1","author":"Dave","year":"2025","journal-title":"Biomaterials"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0195","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0147-6513(03)00083-6","article-title":"Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability","volume":"57","author":"Dayeh","year":"2004","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0200","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/s41420-021-00545-6","article-title":"Plasma membrane permeabilization following cell death: many ways to dye!","volume":"7","author":"De Schutter","year":"2021","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0205","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s10616-012-9481-y","article-title":"Beyond annexin V: fluorescence response of cellular membranes to apoptosis","volume":"65","author":"Demchenko","year":"2013","journal-title":"Cytotechnology"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0210","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s40259-017-0258-x","article-title":"Applications of bioengineered 3D tissue and tumor organoids in drug development and precision medicine: current and future","volume":"32","author":"Devarasetty","year":"2018","journal-title":"BioDrugs"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0215","doi-asserted-by":"crossref","first-page":"2964","DOI":"10.3390\/ijms26072964","article-title":"Synergistic effects of Oxaliplatin, 5-fluorouracil, and novel synthetic uracil analog U-359 on breast Cancer cell carcinogenesis","volume":"26","author":"D\u0142ugosz-Pokorska","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0220","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1089\/ten.tec.2021.0060","article-title":"Cell viability assays in three-dimensional hydrogels: a comparative study of accuracy","volume":"27","author":"Dominijanni","year":"2021","journal-title":"Tissue Eng. Part C Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0225","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1089\/adt.2015.669","article-title":"Bioluminescent, nonlytic, real-time cell viability assay and use in inhibitor screening","volume":"13","author":"Duellman","year":"2015","journal-title":"Assay Drug Dev. Technol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0230","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: a review of programmed cell death","volume":"35","author":"Elmore","year":"2007","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0235","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jff.2008.09.014","article-title":"A modified methylene blue assay for accurate cell counting","volume":"1","author":"Felice","year":"2009","journal-title":"J. Funct. Foods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0240","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0022-1759(75)90064-2","article-title":"Intracellular supravital stain delocalization as an assay for antibody-dependent complement-mediated cell damage","volume":"6","author":"Filman","year":"1975","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0245","doi-asserted-by":"crossref","first-page":"2924","DOI":"10.3390\/cancers16162924","article-title":"The proapoptotic action of pyrrolidinedione\u2013thiazolidinone hybrids towards human breast carcinoma cells does not depend on their genotype","volume":"16","author":"Finiuk","year":"2024","journal-title":"Cancers"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0250","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.toxlet.2005.07.001","article-title":"In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride","volume":"160","author":"Fotakis","year":"2006","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0255","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1038\/nprot.2006.339","article-title":"Clonogenic assay of cells in vitro","volume":"1","author":"Franken","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0260","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1002\/jnr.10825","article-title":"Glutamate uptake inhibitor L-trans-pyrrolidine 2, 4-dicarboxylate becomes neurotoxic in the presence of subthreshold concentrations of mitochondrial toxin 3-nitropropionate: involvement of mitochondrial reducing activity and ATP production","volume":"74","author":"Garc\u00eda","year":"2003","journal-title":"J. Neurosci. Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0265","doi-asserted-by":"crossref","DOI":"10.1007\/s10895-024-03723-8","article-title":"Photo-physical characteristics of Janus Green B in different solvents and its interaction mechanism with silver nanoparticles","author":"Gawad","year":"2024","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0270","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222312827","article-title":"The MTT assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis","volume":"22","author":"Ghasemi","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0275","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0887-2333(01)00014-5","article-title":"Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity","volume":"15","author":"Gonzalez","year":"2001","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0280","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a041012","article-title":"Caspases and their substrates","volume":"14","author":"Green","year":"2022","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0285","doi-asserted-by":"crossref","first-page":"3584","DOI":"10.1038\/s41467-025-58779-2","article-title":"Unveiling cellular communications through rapid pan-membrane-protein labeling","volume":"16","author":"Gunasekara","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0290","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1006\/abio.1997.2220","article-title":"Fluorometric and colorimetric detection of caspase activity associated with apoptosis","volume":"251","author":"Gurtu","year":"1997","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0295","series-title":"Presented at the Proceedings of the 10th International Symposium on Bioluminescence and Chemiluminescence","first-page":"392","article-title":"Stabilization of firefly luciferase using directed evolution","author":"Hall","year":"1998"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0300","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/(SICI)1097-0320(20000601)40:2<167::AID-CYTO11>3.0.CO;2-1","article-title":"A new four-color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells","volume":"40","author":"Hasper","year":"2000","journal-title":"Cytometry"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0305","series-title":"The Handbook: A Guide to Fluorescent Probes and Labeling Technologies","author":"Haugland","year":"2005"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0310","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0091-679X(01)63013-9","article-title":"Protein labeling with fluorescent probes","volume":"63","author":"Holmes","year":"2001","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0315","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-96707-y","article-title":"Enhancing the anticancer effects of rosmarinic acid in PC3 and LNCaP prostate cancer cells using titanium oxide and selenium-doped graphene oxide nanoparticles","volume":"15","author":"Hosseinzadeh Ranjbar","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0320","doi-asserted-by":"crossref","first-page":"4230","DOI":"10.1002\/chem.202003697","article-title":"Cyanine dyes containing quinoline moieties: history, synthesis, optical properties, and applications","volume":"27","author":"Ilina","year":"2021","journal-title":"Chem Eur J"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0325","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1007\/s10971-024-06531-5","article-title":"Multifunctional zinc oxide nanoparticles: investigating antifungal, cytotoxic, and oxidative properties","volume":"112","author":"Javid","year":"2024","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0330","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jim.2005.07.024","volume":"306","author":"Jayaraman","year":"2005","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0335","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1073\/pnas.77.2.990","article-title":"Localization of mitochondria in living cells with rhodamine 123","volume":"77","author":"Johnson","year":"1980","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0340","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/fft2.44","article-title":"Guidelines for cell viability assays","volume":"1","author":"Kamiloglu","year":"2020","journal-title":"Food Front."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0345","series-title":"Time-and Dose-Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay. Detection of Cell Death Mechanisms: Methods and Protocols","first-page":"77","author":"Karassina","year":"2021"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0350","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1007\/s00216-021-03471-9","article-title":"A single-cell analytical approach to quantify activated caspase-3\/7 during osteoblast proliferation, differentiation, and apoptosis","volume":"413","author":"Killinger","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0355","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ab.2015.09.010","article-title":"Application of a non-hazardous vital dye for cell counting with automated cell counters","volume":"492","author":"Kim","year":"2016","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0360","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0022-1759(00)00232-5","article-title":"Detection of dead cells and measurement of cell killing by flow cytometry","volume":"243","author":"King","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0365","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0022-1759(83)90438-6","article-title":"An enzyme-release assay for natural cytotoxicity","volume":"64","author":"Korzeniewski","year":"1983","journal-title":"J. Immunol. Methods"},{"issue":"3","key":"10.1016\/j.biotechadv.2025.108764_bb0370","doi-asserted-by":"crossref","first-page":"170","DOI":"10.2298\/VSP241004010K","article-title":"Anti-inflammatory properties of an A3 adenosine receptor agonist, piclidenoson, in a model of human peripheral blood mononuclear cell culture","volume":"82","author":"Kozi\u0107","year":"2025","journal-title":"Vojnosanit. Pregl."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0375","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1177\/32.10.6090533","article-title":"Fluorescent erythrosin B is preferable to trypan blue as a vital exclusion dye for mammalian cells in monolayer culture","volume":"32","author":"Krause","year":"1984","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0380","article-title":"Analysis of cell viability by the lactate dehydrogenase assay","volume":"2018","author":"Kumar","year":"2018","journal-title":"Cold Spring Harb Protoc"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0385","series-title":"Cytotoxicity Detection Kit (LDH) from Roche Applied Science","author":"Kumarasuriyar","year":"2007"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0390","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fphar.2018.00006","article-title":"Three-dimensional in vitro cell culture models in drug discovery and drug repositioning","volume":"9","author":"Langhans","year":"2018","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0395","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.canlet.2008.05.040","article-title":"Mitochondria-cytochrome C-caspase-9 cascade mediates isorhamnetin-induced apoptosis","volume":"270","author":"Lee","year":"2008","journal-title":"Cancer Lett."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0400","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1155\/1999\/176515","article-title":"Critical evaluation of techniques to detect and measure cell death\u2013study in a model of UV radiation of the leukaemic cell line HL60","volume":"19","author":"Leite","year":"1999","journal-title":"Anal. Cell. Pathol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0405","article-title":"Becoming a CRO expert for human-based cell analytics [WWW document]","author":"Lickiss","year":"2025","journal-title":"Cell. Signal."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0410","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0887-2333(96)00015-X","article-title":"A combination of fluorescent probes for evaluation of cytotoxicity and toxic mechanisms in isolated rainbow trout hepatocytes","volume":"10","author":"Lilius","year":"1996","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0415","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1038\/nprot.2008.114","article-title":"The fluorometric microculture cytotoxicity assay","volume":"3","author":"Lindhagen","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0420","series-title":"Methods in Cell Biology","first-page":"81","article-title":"Evaluation of CAR-T cell cytotoxicity: real-time impedance-based analysis","author":"Lisby","year":"2022"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0425","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1071\/RD21355","article-title":"Assessment of sperm mitochondrial activity by flow cytometry and fluorescent microscopy: a comparative study of mitochondrial fluorescent probes in bovine spermatozoa","volume":"34","author":"Llavanera","year":"2022","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0430","doi-asserted-by":"crossref","DOI":"10.3389\/ftox.2022.981701","article-title":"The alamar blue assay in the context of safety testing of nanomaterials","volume":"4","author":"Longhin","year":"2022","journal-title":"Front. Toxicol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0435","series-title":"DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols","first-page":"3","article-title":"In situ detection of apoptosis by the TUNEL assay: an overview of techniques","author":"Loo","year":"2010"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0440","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1186\/s12967-024-05838-9","article-title":"Exploring the role of PARP1 inhibition in enhancing antibody\u2013drug conjugate therapy for acute leukemias: insights from DNA damage response pathway interactions","volume":"22","author":"Luserna","year":"2024","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0445","doi-asserted-by":"crossref","first-page":"220","DOI":"10.3390\/ijms26010220","article-title":"An overview of the current state of cell viability assessment methods using OECD classification","volume":"26","author":"Madorran","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0450","first-page":"1","article-title":"Zinc supplementation reduces ROS production and prevents MDMA-induced apoptosis in TM3 Leydig cells via the inhibition of pro-apoptotic proteins","author":"Mahmoudi-Nejad","year":"2024","journal-title":"Biol. Trace Elem. Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0455","doi-asserted-by":"crossref","first-page":"6668","DOI":"10.1021\/acs.jpclett.4c00888","article-title":"Uncovering the dissociative adsorption of the leveler Janus Green B on cu electrodes at the molecular level","volume":"15","author":"Mao","year":"2024","journal-title":"J. Phys. Chem. Lett."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0460","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.3233\/CH-248101","article-title":"MTS, WST-8, and ATP viability assays in 2D and 3D cultures: comparison of methodologically different assays in primary human chondrocytes","volume":"88","author":"Martin","year":"2024","journal-title":"Clin. Hemorheol. Microcirc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0465","first-page":"271","article-title":"Limitations of the fluorescent probe viability assay","volume":"2","author":"Massaro","year":"1989","journal-title":"Mol. Toxicol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0470","doi-asserted-by":"crossref","first-page":"5.1.1","DOI":"10.1002\/cpim.40","article-title":"Flow cytometry: an overview","volume":"120","author":"McKinnon","year":"2018","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0475","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1008630","article-title":"Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning","volume":"17","author":"Mergenthaler","year":"2021","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0480","first-page":"4361","article-title":"Basis for the selective cytotoxicity of rhodamine 123","volume":"47","author":"Modica-Napolitano","year":"1987","journal-title":"Cancer Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0485","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1251\/bpo137","article-title":"In vivo and in vitro techniques for comparative study of antiviral T-cell responses in the amphibian Xenopus","volume":"10","author":"Morales","year":"2008","journal-title":"Biological Procedures Online"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0490","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0495","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.3390\/cells13221838","article-title":"Apoptosis: a comprehensive overview of signaling pathways, morphological changes, and physiological significance and therapeutic implications","volume":"13","author":"Mustafa","year":"2024","journal-title":"Cells"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0500","series-title":"Live Quantification of Cell Viability Via Neutral Red Uptake Using lens-Free Imaging","author":"Nablo","year":"2019"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0505","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.procbio.2023.04.027","article-title":"Ultraperformance liquid chromatography-based metabolite profiling and cytotoxic activity of the ethyl acetate extract of two endophytic Penicillium sp","volume":"130","author":"Naveen","year":"2023","journal-title":"Process Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0510","doi-asserted-by":"crossref","first-page":"248","DOI":"10.3390\/antiox12020248","article-title":"Comparative analysis of the antioxidant, antidiabetic, antibacterial, cytoprotective potential and metabolite profile of two endophytic Penicillium spp","volume":"12","author":"Naveen","year":"2023","journal-title":"Antioxidants"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0515","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1007\/BF01962793","article-title":"The pharmacological relevance of vital staining with neutral red","volume":"35","author":"Nemes","year":"1979","journal-title":"Experientia"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0520","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1403272","article-title":"Dual roles of CD11b+ CD33+ HLA-DR-\/lowCD14-myeloid-derived suppressor cells with a granulocytic morphology following allogeneic hematopoietic stem cell transplantation: from inflammation promoters to immune suppressors within 90 days","volume":"15","author":"Ni","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0525","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1002\/cyto.a.24211","article-title":"Evaluating spectral cytometry for immune profiling in viral disease","volume":"97","author":"Niewold","year":"2020","journal-title":"Cytometry A"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0530","first-page":"12","article-title":"Multiplexed viability, cytotoxicity and apoptosis assays for cell-based screening","volume":"16","author":"Niles","year":"2006","journal-title":"Cell Notes"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0535","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ab.2007.04.007","article-title":"A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers","volume":"366","author":"Niles","year":"2007","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0540","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1021\/acsbiomaterials.0c01511","article-title":"Combination therapy of breast cancer by codelivery of doxorubicin and survivin siRNA using polyethylenimine modified silk fibroin nanoparticles","volume":"7","author":"Norouzi","year":"2021","journal-title":"ACS Biomater Sci. Eng."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0545","doi-asserted-by":"crossref","first-page":"S127","DOI":"10.1093\/annonc\/5.suppl_1.S127","article-title":"In vitro drug sensitivity testing of tumor cells from patients with non-Hodgkin's lymphoma using the fluorometric microculture cytotoxicity assay","volume":"5","author":"Nygren","year":"1994","journal-title":"Ann. Oncol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0550","doi-asserted-by":"crossref","first-page":"5421","DOI":"10.1046\/j.1432-1327.2000.01606.x","article-title":"Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity","volume":"267","author":"O'Brien","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0555","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/0959-8049(93)90297-S","article-title":"Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: a further pitfall in the use of the MTT assay for evaluating cell growth and survival","volume":"29","author":"Pagliacci","year":"1993","journal-title":"Eur. J. Cancer"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0560","doi-asserted-by":"crossref","first-page":"2946","DOI":"10.1016\/j.biomaterials.2007.02.034","article-title":"The effect of nano-and micron-sized particles of cobalt\u2013chromium alloy on human fibroblasts in vitro","volume":"28","author":"Papageorgiou","year":"2007","journal-title":"Biomaterials"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0565","doi-asserted-by":"crossref","first-page":"3054","DOI":"10.1016\/j.cgh.2020.06.008","article-title":"Putative mechanisms of diarrhea in COVID-19","volume":"18","author":"Perisetti","year":"2020","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0570","doi-asserted-by":"crossref","first-page":"98","DOI":"10.2144\/000113610","article-title":"Mitochondrial membrane potential probes and the proton gradient: a practical usage guide","volume":"50","author":"Perry","year":"2011","journal-title":"Biotechniques"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0575","doi-asserted-by":"crossref","first-page":"36040","DOI":"10.1074\/jbc.M113.491324","article-title":"NAT8L (N-acetyltransferase 8-like) accelerates lipid turnover and increases energy expenditure in brown adipocytes","volume":"288","author":"Pessentheiner","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0580","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3390\/bios14040156","article-title":"Standard operating procedure to optimize resazurin-based viability assays","volume":"14","author":"Petiti","year":"2024","journal-title":"Biosensors"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0585","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/B978-0-12-427150-0.50101-7","article-title":"Dye exclusion tests for cell viability","author":"Phillips","year":"1973","journal-title":"Tissue Culture. Elsevier"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0590","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1093\/nar\/gkad1091","article-title":"Quantification of all 12 canonical ribonucleotides by real-time fluorogenic in vitro transcription","volume":"52","author":"Purhonen","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0595","article-title":"The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation","volume":"2259","author":"Quah","year":"2010","journal-title":"J. Visual. Exp."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0600","doi-asserted-by":"crossref","first-page":"3452","DOI":"10.1039\/D1NR08429A","article-title":"Triggering the nanophase separation of albumin through multivalent binding to glycogen for drug delivery in 2D and 3D multicellular constructs","volume":"14","author":"Radziwon","year":"2022","journal-title":"Nanoscale"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0605","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s11033-024-10200-9","article-title":"Aqueous and ethanolic extracts of Moringa oleifera leaves induce selective cytotoxicity in Raji and Jurkat cell lines by activating the P21 pathway independent of P53","volume":"52","author":"Rajabi","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0610","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.ymeth.2013.03.030","article-title":"BAK\/BAX activation and cytochrome c release assays using isolated mitochondria","volume":"61","author":"Renault","year":"2013","journal-title":"Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0615","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nprot.2008.75","article-title":"Neutral red uptake assay for the estimation of cell viability\/cytotoxicity","volume":"3","author":"Repetto","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0620","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/154065804322966315","article-title":"Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays","volume":"2","author":"Riss","year":"2004","journal-title":"Assay Drug Dev. Technol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0625","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-61779-108-6_12","article-title":"Cytotoxicity testing: measuring viable cells, dead cells, and detecting mechanism of cell death","author":"Riss","year":"2011","journal-title":"Mammal. Cell Viabil. Methods Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0630","series-title":"Cell Viability Assays. Assay Guidance Manual [Internet]","author":"Riss","year":"2016"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0635","author":"Riss"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0640","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.3390\/cells12141875","article-title":"Flow cytometry: the next revolution","volume":"12","author":"Robinson","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0645","series-title":"Cell Viability Assays: Methods and Protocols","first-page":"237","article-title":"Neutral red uptake assay to assess cytotoxicity in vitro","author":"Rodrigues","year":"2023"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0650","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.jid.2015.11.020","article-title":"Research techniques made simple: techniques to assess cell proliferation","volume":"136","author":"Romar","year":"2016","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0655","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1007\/s10541-005-0104-5","article-title":"Lipophilic triphenylphosphonium cations as tools in mitochondrial bioenergetics and free radical biology","volume":"70","author":"Ross","year":"2005","journal-title":"Biochem. Mosc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0660","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1128\/aem.63.6.2421-2431.1997","article-title":"Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain","volume":"63","author":"Roth","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0665","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1073\/pnas.55.1.134","article-title":"Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters","volume":"55","author":"Rotman","year":"1966","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0670","doi-asserted-by":"crossref","first-page":"5805","DOI":"10.1007\/s12035-023-03433-5","article-title":"A review on caspases: key regulators of biological activities and apoptosis","volume":"60","author":"Sahoo","year":"2023","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0675","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/S0006-3495(85)83825-X","article-title":"Motion of mitochondria in cultured cells quantified by analysis of digitized images","volume":"48","author":"Salmeen","year":"1985","journal-title":"Biophys. J."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0680","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13044-021-00118-w","article-title":"Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma","volume":"14","author":"Samimi","year":"2021","journal-title":"Thyroid. Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0685","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0006-3495(99)77214-0","article-title":"Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives","volume":"76","author":"Scaduto","year":"1999","journal-title":"Biophys. J."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0690","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1021\/tx025558u","article-title":"Surface modification of quartz inhibits toxicity, particle uptake, and oxidative DNA damage in human lung epithelial cells","volume":"15","author":"Schins","year":"2002","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0695","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/cyto.990130216","article-title":"Dead cell discrimination with 7-amino-actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry","volume":"13","author":"Schmid","year":"1992","journal-title":"Cytometry"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0700","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ab.2005.06.009","article-title":"Application of Alamar blue\/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout","volume":"344","author":"Schreer","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0705","series-title":"Animal Cell Technology: Developments Towards the 21st Century","first-page":"1133","article-title":"Evaluation of erythrocin B as a substitute for trypan blue","author":"Scott","year":"1995"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0710","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2024.103159","article-title":"Protocol for real-time assessment of mitochondrial and glycolytic ATP production in patient-derived glioma stem-like cells","volume":"5","author":"Sharma","year":"2024","journal-title":"STAR Protoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0715","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/s41592-023-02042-3","article-title":"A practitioner's view of spectral flow cytometry","volume":"21","author":"Sharma","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0720","article-title":"Pexidartinib impairs liver mitochondrial functions causing cell death in primary human hepatocytes at clinically relevant concentrations","volume":"152075","author":"Shi","year":"2025","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0725","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1021\/ja068008h","article-title":"Fluorescent biomembrane probe for ratiometric detection of apoptosis","volume":"129","author":"Shynkar","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0730","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.3390\/ma12132125","article-title":"Real-time live-cell imaging technology enables high-throughput screening to verify in vitro biocompatibility of 3D printed materials","volume":"12","author":"Siller","year":"2019","journal-title":"Materials"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0735","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.3128","article-title":"Analysis of the mitochondrial membrane potential using the cationic JC-1 dye as a sensitive fluorescent probe","volume":"9","author":"Sivandzade","year":"2019","journal-title":"Bio-protocol"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0740","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1073\/pnas.88.9.3671","article-title":"Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1","volume":"88","author":"Smiley","year":"1991","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0745","doi-asserted-by":"crossref","first-page":"A3-B","DOI":"10.1002\/0471142735.ima03bs111","article-title":"Trypan blue exclusion test of cell viability","volume":"111","author":"Strober","year":"2015","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0750","series-title":"Cellular Chemosensitivity Assays: An Overview. Cancer Cell Culture: Methods and Protocols","first-page":"219","author":"Sumantran","year":"2011"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0755","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s44187-024-00177-6","article-title":"Ethnobotany, total polyphenol content, and cytotoxicity of African olive (Canarium schweinfurthii Engl.) fruit pulp","volume":"4","author":"Tabula","year":"2024","journal-title":"Discov. Food"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0760","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/0014-4827(68)90475-8","article-title":"Deposition of Janus green B and pinocyanol in mitochondria of supravitally stained KB cells","volume":"52","author":"Tanaka","year":"1968","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0765","doi-asserted-by":"crossref","DOI":"10.1016\/j.jare.2024.12.044","article-title":"LTX-315 is a novel broad-spectrum antimicrobial peptide against clinical multidrug-resistant bacteria","author":"Tang","year":"2025","journal-title":"J. Adv. Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0770","doi-asserted-by":"crossref","DOI":"10.1007\/s13167-024-00394-0","article-title":"Identifying specificity protein 2 as a key marker for diabetic encephalopathy in the context of predictive, preventive, and personalized medicine","author":"Tao","year":"2025","journal-title":"EPMA J."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0775","first-page":"9","article-title":"A violet ratiometric membrane probe for the detection of apoptosis","volume":"59","author":"Telford","year":"2012","journal-title":"Curr. Protoc. Cytom."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0780","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-1-4939-3588-8_4","article-title":"Measurement of apoptosis by multiparametric flow cytometry","author":"Telford","year":"2016","journal-title":"Apoptos. Methods Toxicol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0785","doi-asserted-by":"crossref","DOI":"10.1155\/nri\/8948290","article-title":"Inhibition of the transforming growth factor-\u03b2 signaling pathway confers neuroprotective effects on beta-amyloid-induced direct neurotoxicity and microglia-mediated Neuroinflammation","volume":"2025","author":"Tiong","year":"2025","journal-title":"Neurol. Res. Int."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0790","first-page":"184","article-title":"Comparison of MTT, XTT and a novel tetrazolium compound MTS for in-vitro proliferation and chemosensitivity assays","volume":"3","author":"TL","year":"1992","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0795","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.115172","article-title":"Metabolic reprogramming of macrophages by PKM2 promotes IL-10 production via adenosine","volume":"44","author":"Toller-Kawahisa","year":"2025","journal-title":"Cell Rep."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0800","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/BF00914435","article-title":"A method for viable cell count","volume":"1","author":"Tolnai","year":"1975","journal-title":"TCA Manual\/Tissue Culture Assoc."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0805","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0003-9861(02)00217-5","article-title":"Dipeptidyl peptidase I: importance of progranzyme activation sequences, other dipeptide sequences, and the N-terminal amino group of synthetic substrates for enzyme activity","volume":"403","author":"Tran","year":"2002","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0810","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1159\/000077285","article-title":"Interference by anti-cancer chemotherapeutic agents in the MTT-tumor chemosensitivity assay","volume":"50","author":"Ulukaya","year":"2004","journal-title":"Chemotherapy"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0815","author":"US Environmental Protection Agency"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0820","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.3390\/nano10112236","article-title":"From 2D to 3D cancer cell models\u2014the enigmas of drug delivery research","volume":"10","author":"Van Zundert","year":"2020","journal-title":"Nanomaterials"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0825","doi-asserted-by":"crossref","DOI":"10.3390\/cells14010044","article-title":"New insights in microplastic cellular uptake through a cell-based Organotypic rainbow-trout (Oncorhynchus mykiss) intestinal platform","volume":"14","author":"Verdile","year":"2025","journal-title":"Cells"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0830","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1084\/jem.182.5.1597","article-title":"Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes","volume":"182","author":"Verhoven","year":"1995","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0835","doi-asserted-by":"crossref","first-page":"21200","DOI":"10.1021\/acs.est.3c05675","article-title":"Microplate-based cell viability assay as a cost-effective alternative to flow cytometry for microalgae analysis","volume":"57","author":"Wang","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0840","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/sj.cdd.4401263","article-title":"A new quantitative assay for cytochrome c release in apoptotic cells","volume":"10","author":"Waterhouse","year":"2003","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0845","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioadv.2022.213083","article-title":"Influence of protein corona on the interaction of glycogen\u2013siRNA constructs with ex vivo human blood immune cells","volume":"140","author":"Wojnilowicz","year":"2022","journal-title":"Biomater. Adv."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0850","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.3390\/bios12111012","article-title":"Colorimetric detection of DNase type I 3\u2032 OH DNA ends using an isothermal amplification-assisted paper-based analytical device","volume":"12","author":"Xue","year":"2022","journal-title":"Biosensors"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0855","article-title":"Extracellular flux assay (seahorse assay): diverse applications in metabolic research across biological disciplines","volume":"100095","author":"Yoo","year":"2024","journal-title":"Mol. Cell"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0860","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/toxres\/tfab114","article-title":"JC-10 probe as a novel method for analyzing the mitochondrial membrane potential and cell stress in whole zebrafish embryos","volume":"11","author":"Younes","year":"2022","journal-title":"Toxicol. Res."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0865","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.89826","article-title":"An open-source, high-resolution, automated fluorescence microscope","volume":"12","author":"Zehrer","year":"2024","journal-title":"Elife"},{"key":"10.1016\/j.biotechadv.2025.108764_bb0870","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1038\/s41598-025-86792-4","article-title":"Multi-assay assessment of cytotoxicity reveals multiple mechanisms of action in 3D microtissues","volume":"15","author":"Zein-Sabatto","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0875","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/s41378-023-00562-8","article-title":"Computer vision meets microfluidics: a label-free method for high-throughput cell analysis","volume":"9","author":"Zhou","year":"2023","journal-title":"Microsyst. Nanoeng."},{"key":"10.1016\/j.biotechadv.2025.108764_bb0880","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2024.125323","article-title":"Short-chain chlorinated paraffins induce liver injury in mice through mitochondrial disorders and disruption of cholesterol-bile acid pathway","volume":"364","author":"Zhou","year":"2025","journal-title":"Environ. Pollut."}],"container-title":["Biotechnology Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0734975025002502?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0734975025002502?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T11:07:05Z","timestamp":1773832025000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0734975025002502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":176,"alternative-id":["S0734975025002502"],"URL":"https:\/\/doi.org\/10.1016\/j.biotechadv.2025.108764","relation":{},"ISSN":["0734-9750"],"issn-type":[{"value":"0734-9750","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"From dye exclusion to high-throughput screening: A review of cell viability assays and their applications","name":"articletitle","label":"Article Title"},{"value":"Biotechnology Advances","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biotechadv.2025.108764","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"108764"}}