{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:15:04Z","timestamp":1792516504268,"version":"4.5.0"},"reference-count":84,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013061","name":"Jilin Scientific and Technological Development Program","doi-asserted-by":"publisher","award":["20250203114SF"],"award-info":[{"award-number":["20250203114SF"]}],"id":[{"id":"10.13039\/501100013061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302874"],"award-info":[{"award-number":["32302874"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Advances"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.biotechadv.2026.109017","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:55:06Z","timestamp":1787241306000},"page":"109017","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["CRISPR\/Cas trans-cleavage activity in pathogen detection: research progress and innovations"],"prefix":"10.1016","volume":"93","author":[{"given":"Rong","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biotechadv.2026.109017_bb0005","author":"AAT Bioquest"},{"issue":"7675","key":"10.1016\/j.biotechadv.2026.109017_bb0010","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/nature24049","article-title":"RNA targeting with CRISPR-Cas13","volume":"550","author":"Abudayyeh","year":"2017","journal-title":"Nature"},{"issue":"7811","key":"10.1016\/j.biotechadv.2026.109017_bb0015","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/s41586-020-2279-8","article-title":"Massively multiplexed nucleic acid detection with Cas13","volume":"582","author":"Ackerman","year":"2020","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0020","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkag161","article-title":"A novel dual-guide CRISPR\u2013Cas13 strategy improves specificity for single-nucleotide variant detection","volume":"54","author":"Aguilar-Gonz\u00e1lez","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0025","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.nano.2019.03.006","article-title":"CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic","volume":"18","author":"Aquino-Jarquin","year":"2019","journal-title":"Nanomedicine"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0030","doi-asserted-by":"crossref","first-page":"5921","DOI":"10.1038\/s41467-020-19097-x","article-title":"Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2","volume":"11","author":"Arizti-Sanz","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113207","article-title":"Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis","volume":"183","author":"Azhar","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"3","key":"10.1016\/j.biotechadv.2026.109017_bb0040","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s00294-019-01040-3","article-title":"A glance at genome editing with CRISPR-Cas9 technology","volume":"66","author":"Barman","year":"2020","journal-title":"Curr. Genet."},{"issue":"6387","key":"10.1016\/j.biotechadv.2026.109017_bb0045","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.biotechadv.2026.109017_bb0050","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41587-024-02255-7","article-title":"Trans-nuclease activity of Cas9 activated by DNA or RNA target binding","volume":"43","author":"Chen","year":"2025","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0055","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1021\/acs.nanolett.0c04303","article-title":"CRISPR-Cas12a-based nucleic acid amplification-free DNA biosensor via au nanoparticle-assisted metal-enhanced fluorescence and colorimetric analysis","volume":"21","author":"Choi","year":"2021","journal-title":"Nano Lett."},{"issue":"48","key":"10.1016\/j.biotechadv.2026.109017_bb0060","doi-asserted-by":"crossref","first-page":"17399","DOI":"10.1002\/anie.201910772","article-title":"Exploring the trans-cleavage activity of CRISPR-Cas12a (cpf1) for the development of a universal electrochemical biosensor","volume":"58","author":"Dai","year":"2019","journal-title":"Angew. Chem. Int. Ed. Eng."},{"issue":"44","key":"10.1016\/j.biotechadv.2026.109017_bb0065","doi-asserted-by":"crossref","first-page":"16089","DOI":"10.1021\/acs.analchem.3c01861","article-title":"Multiplexed CRISPR-based nucleic acid detection using a single Cas protein","volume":"95","author":"Fu","year":"2023","journal-title":"Anal. Chem."},{"issue":"6336","key":"10.1016\/j.biotechadv.2026.109017_bb0070","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"issue":"6387","key":"10.1016\/j.biotechadv.2026.109017_bb0075","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0080","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.bios.2017.04.016","article-title":"A facile, rapid and sensitive detection of MRSA using a CRISPR-mediated DNA FISH method, antibody-like dCas9\/sgRNA complex","volume":"95","author":"Guk","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.133401","article-title":"Cas12a-based primer production enables isothermal amplification for nucleic acid detection","volume":"381","author":"Han","year":"2023","journal-title":"Sens. Actuators B"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115323","article-title":"Cas12a\/blocker DNA-based multiplex nucleic acid detection system for diagnosis of high-risk human papillomavirus infection","volume":"232","author":"Han","year":"2023","journal-title":"Biosens. Bioelectron."},{"issue":"6416","key":"10.1016\/j.biotechadv.2026.109017_bb0095","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.aav4294","article-title":"Programmed DNA destruction by miniature CRISPR-Cas14 enzymes","volume":"362","author":"Harrington","year":"2018","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0100","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/s44328-025-00062-x","article-title":"Sustainable and portable CRISPR-based diagnostics for high-sensitivity Mpox detection","volume":"2","author":"Hirano","year":"2025","journal-title":"npj Biosens."},{"issue":"3","key":"10.1016\/j.biotechadv.2026.109017_bb0105","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1021\/acs.analchem.7b04542","article-title":"Clustered regularly interspaced short palindromic repeats\/Cas9 triggered isothermal amplification for site-specific nucleic acid detection","volume":"90","author":"Huang","year":"2018","journal-title":"Anal. Chem."},{"issue":"4","key":"10.1016\/j.biotechadv.2026.109017_bb0110","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.214","article-title":"Deep learning enhancing guide RNA design for CRISPR\/Cas12a-based diagnostics","volume":"3","author":"Huang","year":"2024","journal-title":"iMeta"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0115","doi-asserted-by":"crossref","first-page":"8257","DOI":"10.1038\/s41467-025-63094-x","article-title":"Sensitive pathogen DNA detection by a multi-guide RNA Cas12a assay favoring trans- versus cis-cleavage","volume":"16","author":"Huang","year":"2025","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0120","first-page":"790","article-title":"On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay","volume":"24","author":"Jin","year":"2025","journal-title":"J. Integr. Agric."},{"issue":"6096","key":"10.1016\/j.biotechadv.2026.109017_bb0125","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA\u2013guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"issue":"15","key":"10.1016\/j.biotechadv.2026.109017_bb0130","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1056\/NEJMc2026172","article-title":"Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing","volume":"383","author":"Joung","year":"2020","journal-title":"N. Engl. J. Med."},{"issue":"7","key":"10.1016\/j.biotechadv.2026.109017_bb0135","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"issue":"9","key":"10.1016\/j.biotechadv.2026.109017_bb0140","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.1093\/nar\/gkaa208","article-title":"PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage","volume":"48","author":"Karvelis","year":"2020","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.biotechadv.2026.109017_bb0145","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1021\/acs.analchem.3c05459","article-title":"On-site fluorescent detection of sepsis-inducing bacteria using a graphene-oxide CRISPR-Cas12a (GO-CRISPR) system","volume":"96","author":"Kasputis","year":"2024","journal-title":"Anal. Chem."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0150","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.molcel.2016.03.024","article-title":"Spatiotemporal control of type III-A CRISPR-Cas immunity: coupling DNA degradation with the target RNA recognition","volume":"62","author":"Kazlauskiene","year":"2016","journal-title":"Mol. Cell"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2024.126864","article-title":"Enhancing multiplex detection capabilities of the Cas12a\/blocker DNA system","volume":"281","author":"Kim","year":"2025","journal-title":"Talanta"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111917","article-title":"CRISPR: history and perspectives to the future","volume":"141","author":"Kozovska","year":"2021","journal-title":"Biomed. Pharmacother."},{"issue":"4","key":"10.1016\/j.biotechadv.2026.109017_bb0165","doi-asserted-by":"crossref","first-page":"R61","DOI":"10.1186\/gb-2007-8-4-r61","article-title":"Evolutionary conservation of sequence and secondary structures in CRISPR repeats","volume":"8","author":"Kunin","year":"2007","journal-title":"Genome Biol."},{"issue":"41","key":"10.1016\/j.biotechadv.2026.109017_bb0170","doi-asserted-by":"crossref","first-page":"25722","DOI":"10.1073\/pnas.2010196117","article-title":"Ultrasensitive CRISPR-based diagnostic for field-applicable detection of plasmodium species in symptomatic and asymptomatic malaria","volume":"117","author":"Lee","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"issue":"4","key":"10.1016\/j.biotechadv.2026.109017_bb0175","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s13206-022-00081-0","article-title":"Highly efficient DNA reporter for CRISPR\/Cas12a-based specific and sensitive biosensor","volume":"16","author":"Lee","year":"2022","journal-title":"Biochip J."},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0180","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1021\/acssynbio.1c00620","article-title":"RPA\/CRISPR\/Cas12a-based on-site and rapid nucleic acid detection of Toxoplasma gondii in the environment","volume":"11","author":"Lei","year":"2022","journal-title":"ACS Synth. Biol."},{"issue":"3","key":"10.1016\/j.biotechadv.2026.109017_bb0185","doi-asserted-by":"crossref","first-page":"354","DOI":"10.33594\/000000224","article-title":"CRISPR\/Cas9 technology in translational biomedicine","volume":"54","author":"Leonova","year":"2020","journal-title":"Cell. Physiol. Biochem."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0195","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted nucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell Discov."},{"issue":"10","key":"10.1016\/j.biotechadv.2026.109017_bb0200","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acssynbio.9b00209","article-title":"HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation","volume":"8","author":"Li","year":"2019","journal-title":"ACS Synth. Biol."},{"issue":"11","key":"10.1016\/j.biotechadv.2026.109017_bb0210","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2420166122","article-title":"Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples","volume":"122","author":"Lim","year":"2025","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0215","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.molcel.2016.11.040","article-title":"C2c1-sgRNA complex structure reveals RNA-guided DNA cleavage mechanism","volume":"65","author":"Liu","year":"2017","journal-title":"Mol. Cell"},{"issue":"1\u20132","key":"10.1016\/j.biotechadv.2026.109017_bb0220","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.cell.2016.12.031","article-title":"Two distant catalytic sites are responsible for C2c2 RNase activities","volume":"168","author":"Liu","year":"2017","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0225","first-page":"44","article-title":"CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation","volume":"12","author":"Liu","year":"2025","journal-title":"Mil. Med. Res."},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0230","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1089\/crispr.2018.0033","article-title":"Classification and nomenclature of CRISPR-Cas systems: where from here?","volume":"1","author":"Makarova","year":"2018","journal-title":"Crispr J."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0235","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1093\/nar\/gkad1132","article-title":"New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity","volume":"52","author":"Molina Vargas","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"6387","key":"10.1016\/j.biotechadv.2026.109017_bb0240","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable viral diagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0245","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ymeth.2021.02.001","article-title":"CRISPR-ENHANCE: an enhanced nucleic acid detection platform using Cas12a","volume":"203","author":"Nguyen","year":"2022","journal-title":"Methods"},{"issue":"6408","key":"10.1016\/j.biotechadv.2026.109017_bb0250","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1126\/science.aas9129","article-title":"Engineered CRISPR-Cas9 nuclease with expanded targeting space","volume":"361","author":"Nishimasu","year":"2018","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0255","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2016.04.059","article-title":"Rapid, low-cost detection of Zika virus using programmable biomolecular components","volume":"165","author":"Pardee","year":"2016","journal-title":"Cell"},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0260","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1089\/crispr.2022.0048","article-title":"A multiplexed Cas13-based assay with point-of-care attributes for simultaneous COVID-19 diagnosis and variant surveillance","volume":"6","author":"Patchsung","year":"2023","journal-title":"Crispr J."},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0265","first-page":"8","article-title":"CRISPR-Cas12a: functional overview and applications","volume":"43","author":"Paul","year":"2020","journal-title":"Biom. J."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0270","article-title":"From PCR to CRISPR: comparing the performance of different nucleic acid detection technologies","volume":"1","author":"Peng","year":"2025","journal-title":"Biomed. Instrum."},{"issue":"17","key":"10.1016\/j.biotechadv.2026.109017_bb0275","doi-asserted-by":"crossref","first-page":"10040","DOI":"10.1093\/nar\/gkae736","article-title":"CRISPR-Cas target recognition for sensing viral and cancer biomarkers","volume":"52","author":"Rahimi","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0280","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.049874","article-title":"New advances in CRISPR\/Cas-mediated precise gene-editing techniques","volume":"16","author":"Richardson","year":"2023","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0285","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2025.138850","article-title":"Onsite detection of airborne antibiotic-resistant bacteria via Cas9 nickase-triggered amplification reactions","volume":"495","author":"Seo","year":"2025","journal-title":"J. Hazard. Mater."},{"issue":"2","key":"10.1016\/j.biotechadv.2026.109017_bb0290","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1039\/D0CC06412B","article-title":"Cascade CRISPR\/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity","volume":"57","author":"Sha","year":"2021","journal-title":"Chem. Commun. (Camb.)"},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0295","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abc7802","article-title":"A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics","volume":"7","author":"Shi","year":"2021","journal-title":"Sci. Adv."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0300","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2022.131871","article-title":"Sensitive and simultaneous detection of hygiene indicator bacteria using an enhanced CRISPR\/Cas system in combination with a portable fluorescence detector","volume":"365","author":"Shin","year":"2022","journal-title":"Sens. Actuators B"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0305","first-page":"242","article-title":"Universal amplification-free RNA detection by integrating CRISPR-Cas10 with aptameric graphene field-effect transistor","volume":"17","author":"Sun","year":"2025","journal-title":"Nanomicro Lett."},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0310","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1186\/s13059-019-1742-z","article-title":"CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity","volume":"20","author":"Teng","year":"2019","journal-title":"Genome Biol."},{"issue":"19","key":"10.1016\/j.biotechadv.2026.109017_bb0315","doi-asserted-by":"crossref","first-page":"12156","DOI":"10.1021\/acs.analchem.9b01526","article-title":"Cas12aVDet: a CRISPR\/Cas12a-based platform for rapid and visual nucleic acid detection","volume":"91","author":"Wang","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0320","doi-asserted-by":"crossref","first-page":"1158163","DOI":"10.3389\/fmicb.2023.1158163","article-title":"CDetection.v2: one-pot assay for the detection of SARS-CoV-2","volume":"14","author":"Wang","year":"2023","journal-title":"Front. Microbiol."},{"issue":"45","key":"10.1016\/j.biotechadv.2026.109017_bb0325","doi-asserted-by":"crossref","first-page":"24241","DOI":"10.1002\/anie.202110384","article-title":"Trans single-stranded DNA cleavage via CRISPR\/Cas14a1 activated by target RNA without destruction","volume":"60","author":"Wei","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0330","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1038\/s41591-022-01734-1","article-title":"Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants","volume":"28","author":"Welch","year":"2022","journal-title":"Nat. Med."},{"issue":"12","key":"10.1016\/j.biotechadv.2026.109017_bb0335","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1038\/s41551-025-01424-6","article-title":"LbuCas13a directly targets DNA and elicits strong trans-cleavage activity","volume":"9","author":"Wu","year":"2025","journal-title":"Nat. Biomed. Eng."},{"issue":"6397","key":"10.1016\/j.biotechadv.2026.109017_bb0340","doi-asserted-by":"crossref","DOI":"10.1126\/science.aat0839","article-title":"Structure basis for RNA-guided DNA degradation by Cascade and Cas3","volume":"361","author":"Xiao","year":"2018","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.biotechadv.2026.109017_bb0345","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/s41592-021-01124-4","article-title":"Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes","volume":"18","author":"Xu","year":"2021","journal-title":"Nat. Methods"},{"issue":"7","key":"10.1016\/j.biotechadv.2026.109017_bb0350","article-title":"Engineering CRISPR\/Cas13 system against RNA viruses: from diagnostics to therapeutics","volume":"9","author":"Xue","year":"2022","journal-title":"Bioengineering (Basel)"},{"issue":"12","key":"10.1016\/j.biotechadv.2026.109017_bb0355","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.102208","article-title":"Facile, ultrasensitive, and highly specific diagnosis of goose astrovirus via reverse transcription-enzymatic recombinase amplification coupled with a CRISPR-Cas12a system detection","volume":"101","author":"Yang","year":"2022","journal-title":"Poult. Sci."},{"issue":"6","key":"10.1016\/j.biotechadv.2026.109017_bb0360","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkag267","article-title":"Elimination of cis-cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection","volume":"54","author":"Yin","year":"2026","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0365","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1186\/s13059-024-03448-8","article-title":"Systemic evaluation of various CRISPR\/Cas13 orthologs for knockdown of targeted transcripts in plants","volume":"25","author":"Yu","year":"2024","journal-title":"Genome Biol."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0370","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.340009","article-title":"Rapid RNA detection through intra-enzyme chain replacement-promoted Cas13a cascade cyclic reaction without amplification","volume":"1217","author":"Zeng","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.biotechadv.2026.109017_bb0375","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ab.2018.09.012","article-title":"Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique","volume":"561-562","author":"Zhang","year":"2018","journal-title":"Anal. Biochem."},{"issue":"3","key":"10.1016\/j.biotechadv.2026.109017_bb0385","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf040","article-title":"Direct repeat region 3\u2032 end modifications regulate Cas12a activity and expand its applications","volume":"53","author":"Zhang","year":"2025","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"10.1016\/j.biotechadv.2026.109017_bib432","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.cell.2015.09.038","article-title":"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system","volume":"163","author":"Zetsche","year":"2015","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0390","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102811","article-title":"Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications","volume":"37","author":"Zhang","year":"2026","journal-title":"Mol. Ther. Nucleic Acids"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0395","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1038\/s41467-025-58945-6","article-title":"Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization","volume":"16","author":"Zhao","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0400","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2024.127457","article-title":"The nucleic acid detection using CRISPR\/Cas biosensing system with micro-nano modality for point-of-care applications","volume":"286","author":"Zhao","year":"2025","journal-title":"Talanta"},{"issue":"13","key":"10.1016\/j.biotechadv.2026.109017_bb0405","doi-asserted-by":"crossref","first-page":"9982","DOI":"10.1021\/acs.analchem.5c08220","article-title":"Direct RNA triggering of Cas12a through the native crRNA architecture enables clinical nucleic acids diagnostics","volume":"98","author":"Zhao","year":"2026","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0410","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41392-022-00893-4","article-title":"Preventing autosomal-dominant hearing loss in Bth mice with CRISPR\/CasRx-based RNA editing","volume":"7","author":"Zheng","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"issue":"13","key":"10.1016\/j.biotechadv.2026.109017_bb0415","article-title":"CRISPR\/Cas13a powered portable electrochemiluminescence chip for ultrasensitive and specific MiRNA detection","volume":"7","author":"Zhou","year":"2020","journal-title":"Adv. Sci. (Weinh.)"},{"issue":"10","key":"10.1016\/j.biotechadv.2026.109017_bb0420","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1039\/D3AN00462G","article-title":"The construction of CRISPR\/Cas9-mediated FRET 16S rDNA sensor for detection of Mycobacterium tuberculosis","volume":"148","author":"Zhou","year":"2023","journal-title":"Analyst"},{"issue":"1","key":"10.1016\/j.biotechadv.2026.109017_bb0425","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1186\/s12985-023-02232-7","article-title":"Rapid detection of avian influenza virus based on CRISPR-Cas12a","volume":"20","author":"Zhou","year":"2023","journal-title":"Virol. J."},{"key":"10.1016\/j.biotechadv.2026.109017_bb0430","article-title":"Challenges and strategies for CRISPR-Cas molecular diagnostics from mechanism to clinical translation","volume":"11","author":"Zhou","year":"2026","journal-title":"Sens. Actuators Rep."}],"container-title":["Biotechnology Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0734975026002235?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0734975026002235?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:24:31Z","timestamp":1790699071000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0734975026002235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":84,"alternative-id":["S0734975026002235"],"URL":"https:\/\/doi.org\/10.1016\/j.biotechadv.2026.109017","relation":{},"ISSN":["0734-9750"],"issn-type":[{"value":"0734-9750","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"CRISPR\/Cas trans-cleavage activity in pathogen detection: research progress and innovations","name":"articletitle","label":"Article Title"},{"value":"Biotechnology Advances","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biotechadv.2026.109017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"109017"}}