{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:21:21Z","timestamp":1778224881585,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T00:00:00Z","timestamp":1463616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Brazilian Journal of Infectious Diseases"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.bjid.2016.04.005","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T01:13:50Z","timestamp":1464657230000},"page":"335-341","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples"],"prefix":"10.1016","volume":"20","author":[{"given":"Ivano","family":"de Filippis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Ferreira","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalia","family":"Caldeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Carvalho","family":"de Azevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Eugenio","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"Suppl. 2","key":"10.1016\/j.bjid.2016.04.005_bib0150","doi-asserted-by":"crossref","first-page":"B71","DOI":"10.1016\/j.vaccine.2009.04.070","article-title":"Biology and pathogenesis of the evolutionarily successful, obligate human bacterium Neisseria meningitidis","volume":"27","author":"Stephens","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.bjid.2016.04.005_bib0155","unstructured":"World Health Organization Communicable Disease Surveillance and Response. Laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenza. http:\/\/www.who.int\/csr\/resources\/publications\/meningitis\/WHO_CDS_CSR_EDC_99_7_EN\/en\/. [Accessed 31.01.16]."},{"issue":"May (5)","key":"10.1016\/j.bjid.2016.04.005_bib0160","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S1473-3099(10)70048-7","article-title":"Global and regional risk of disabling sequelae from bacterial meningitis: a systematic review and meta-analysis","volume":"10","author":"Edmond","year":"2010","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0165","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1128\/JCM.32.11.2738-2744.1994","article-title":"Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy","volume":"32","author":"R\u00e5dstr\u00f6m","year":"1994","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0170","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1128\/JCM.38.2.855-857.2000","article-title":"Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis","volume":"38","author":"Taha","year":"2000","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0175","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1086\/345438","article-title":"Broad-range bacterial polymerase chain reaction for early detection of bacterial meningitis","volume":"36","author":"Saravolatz","year":"2003","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0180","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1469-0691.2005.01109.x","article-title":"Simultaneous single-tube PCR assay or the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae","volume":"11","author":"Tzanakaki","year":"2005","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.bjid.2016.04.005_bib0185","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.diagmicrobio.2004.10.004","article-title":"Rapid detection of Neisseria meningitidis in cerebrospinal fluid by one-step polymerase chain reaction of the nspA gene","volume":"51","author":"de Filippis","year":"2005","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0190","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1111\/j.1469-0691.2007.01756.x","article-title":"Comparison of broad-range bacterial PCR and culture of cerebrospinal fluid for diagnosis of community-acquired bacterial meningitis","volume":"13","author":"Welinder-Olsson","year":"2007","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.bjid.2016.04.005_bib0195","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1080\/00365540600904761","article-title":"Diagnosis of meningococcal meningitis in Brazil by use of PCR","volume":"39","author":"Pedro","year":"2007","journal-title":"Scand J Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0200","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1128\/JCM.39.4.1553-1558.2001","article-title":"Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR","volume":"39","author":"Corless","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0205","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1471-2180-2-17","article-title":"Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae","volume":"9","author":"Mygind","year":"2002","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0210","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1128\/JCM.42.7.2919-2925.2004","article-title":"Prospective study of a real-time PCR that is highly sensitive, specific, and clinically useful for diagnosis of meningococcal disease in children","volume":"42","author":"Bryant","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0215","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/JCM.42.7.2980-2987.2004","article-title":"Use of robotized DNA isolation and real-time PCR to quantify and identify close correlation between levels of Neisseria meningitidis DNA and lipopolysaccharides in plasma and cerebrospinal fluid from patients with systemic meningococcal disease","volume":"42","author":"\u00d8vsteb\u00f8","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0220","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.diagmicrobio.2006.01.007","article-title":"Quantitative detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in lower respiratory tract samples by real-time PCR","volume":"55","author":"Kais","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0225","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.02498-06","article-title":"Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA","volume":"45","author":"Carvalho Mda","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0230","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s10096-007-0350-0","article-title":"Evaluation of a real-time polymerase chain reaction assay for the diagnosis of pneumococcal and meningococcal meningitis in a tertiary care hospital","volume":"26","author":"Van Gastel","year":"2007","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0235","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1099\/00222615-33-4-271","article-title":"Detection of Haemophilus influenzae in cerebrospinal fluids by polymerase chain reaction DNA amplification","volume":"33","author":"van Ketel","year":"1990","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0240","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1128\/JCM.32.10.2382-2386.1994","article-title":"PCR for capsular typing of Haemophilus influenzae","volume":"32","author":"Falla","year":"1994","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0245","doi-asserted-by":"crossref","first-page":"4590","DOI":"10.1128\/JCM.01766-10","article-title":"False-negative PCR result due to gene polymorphism: the example of Neisseria meningitidis","volume":"48","author":"Jaton","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0250","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1128\/JCM.42.2.807-810.2004","article-title":"Emergence of nonencapsulated and encapsulated non-b-type invasive Haemophilus influenzae isolates in Portugal (1989\u20132001)","volume":"42","author":"Bajanca","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0255","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.diagmicrobio.2009.10.005","article-title":"Determination of capsulation status in Haemophilus influenzae by multiplex polymerase chain reaction","volume":"66","author":"Nelson","year":"2010","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0260","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.3201\/eid1709.101991","article-title":"Increasing incidence of invasive Haemophilus influenzae disease in adults, Utah, USA","volume":"17","author":"Rubach","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0265","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1471-2334-9-45","article-title":"Invasive Haemophilus influenzae infections in Germany: impact of non-type b serotypes in the post-vaccine era","volume":"9","author":"Kalies","year":"2009","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0270","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1128\/JCM.42.2.524-529.2004","article-title":"Analysis of invasive Haemophilus influenzae infections after extensive vaccination against H. influenzae type b","volume":"42","author":"Campos","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2016.04.005_bib0275","doi-asserted-by":"crossref","first-page":"e167","DOI":"10.1016\/j.ijid.2010.10.005","article-title":"Invasive Haemophilus influenzae in British Columbia: non-Hib and non-typeable strains causing disease in children and adults","volume":"15","author":"Shuel","year":"2011","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0280","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.1016\/j.vaccine.2010.03.075","article-title":"Changing epidemiology of invasive Haemophilus influenzae in Ontario, Canada: evidence for herd effects and strain replacement due to Hib vaccination","volume":"28","author":"Adam","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.bjid.2016.04.005_bib0285","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/cir268","article-title":"Nontypeable Haemophilus influenzae invasive disease in The Netherlands: a retrospective surveillance study 2001\u20132008","volume":"53","author":"Van Wessel","year":"2011","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.bjid.2016.04.005_bib0290","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2334-12-40","article-title":"Non-capsulated and capsulated Haemophilus influenzae in children with acute otitis media in Venezuela: a prospective epidemiological study","volume":"15","author":"Naranjo","year":"2012","journal-title":"BMC Infect Dis"}],"container-title":["The Brazilian Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1413867016300770?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1413867016300770?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T10:02:55Z","timestamp":1600682575000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1413867016300770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["S1413867016300770"],"URL":"https:\/\/doi.org\/10.1016\/j.bjid.2016.04.005","relation":{},"ISSN":["1413-8670"],"issn-type":[{"value":"1413-8670","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples","name":"articletitle","label":"Article Title"},{"value":"The Brazilian Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bjid.2016.04.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Editora Ltda.","name":"copyright","label":"Copyright"}]}}