{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T15:56:51Z","timestamp":1761407811232,"version":"build-2065373602"},"reference-count":39,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T00:00:00Z","timestamp":1559088000000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Brazilian Journal of Infectious Diseases"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.bjid.2019.05.005","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T08:17:58Z","timestamp":1559722678000},"page":"164-172","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach"],"prefix":"10.1016","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2259-6859","authenticated-orcid":false,"given":"Milene Gon\u00e7alves","family":"Quiles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Cruz","family":"Boettger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Matsiko","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jussimara","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Wagner","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinicius","family":"Ponzio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabianne","family":"Carlesse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Cappellano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia Godoy","family":"Carvalhaes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Carlos Campos","family":"Pignatari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bjid.2019.05.005_bib0200","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1086\/421946","article-title":"Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study","volume":"39","author":"Wisplinghoff","year":"2004","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0205","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/jac\/dkw402","article-title":"Association between inappropriate empirical antimicrobial therapy and hospital length of stay in Gram-negative bloodstream infections: stratification by prognosis","volume":"72","author":"Battle","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.bjid.2019.05.005_bib0210","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jinf.2016.11.001","article-title":"Attributable mortality of ICU-acquired bloodstream infections: Impact of the source, causative micro-organism, resistance profile and antimicrobial therapy","volume":"74","author":"Adrie","year":"2017","journal-title":"J Infect"},{"key":"10.1016\/j.bjid.2019.05.005_bib0215","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1097\/CCM.0b013e3181d69db7","article-title":"Antibiotics in sepsis and septic shock: like everything else in life, timing is everything","volume":"38","author":"Zubert","year":"2010","journal-title":"Crit Care Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0220","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1097\/CCM.0b013e31827e83af","article-title":"Surviving sepsis campaign guidelines committee including the pediatric subgroup Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock 2012","volume":"41","author":"Dellinger","year":"2013","journal-title":"Crit Care Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0225","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1128\/AAC.00462-11","article-title":"SAEI\/SAMPAC Bacteremia Group Impact of inadequate empirical therapy on the mortality of patients with bloodstream infections: a propensity score-based analysis","volume":"56","author":"Retamar","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.bjid.2019.05.005_bib0230","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1097\/00003246-200107000-00002","article-title":"Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care","volume":"29","author":"Angus","year":"2001","journal-title":"Crit Care Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0235","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1097\/01.CCM.0000217961.75225.E9","article-title":"Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock","volume":"34","author":"Kumar","year":"2006","journal-title":"Crit Care Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0240","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1309\/AJCPVMDQU2ZJDPBL","article-title":"Decreased mortality associated with prompt Gram staining of blood cultures","volume":"130","author":"Barenfanger","year":"2008","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0245","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.ijmm.2010.04.006","article-title":"Molecular diagnostics of sepsis \u2013 where are we today?","volume":"300","author":"Bauer","year":"2010","journal-title":"Int J Med Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0250","doi-asserted-by":"crossref","first-page":"330","DOI":"10.7326\/0003-4819-132-4-200002150-00025","article-title":"Rapid identification of pathogens in blood","volume":"132","author":"Kempf","year":"2000","journal-title":"Ann Intern Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0255","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1186\/s12879-015-1033-6","article-title":"Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR","volume":"15","author":"Quiles","year":"2015","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0260","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/1471-2334-13-166","article-title":"Diagnosis by real-time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections","volume":"13","author":"Menezes","year":"2013","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0265","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.00581-15","article-title":"Identification of Gram-negative bacteria and genetic resistance determinants from positive blood culture broths by use of the Verigene Gram-negative blood culture multiplex microarray-based molecular assay","volume":"53","author":"Ledeboer","year":"2015","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0270","doi-asserted-by":"crossref","DOI":"10.1016\/j.mayocp.2016.06.020","article-title":"New developments in clinical bacteriology laboratories","author":"Patel","year":"2016","journal-title":"Mayo Clin Proc"},{"key":"10.1016\/j.bjid.2019.05.005_bib0275","doi-asserted-by":"crossref","DOI":"10.1007\/s40506-017-0104-1","article-title":"Update in bloodstream infections diagnosis using new methods in microbiology","author":"Quiles","year":"2017","journal-title":"Curr Treat Options Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0280","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2016.08.019","article-title":"Optimal turnaround time for direct identification of microorganisms by mass spectrometry in blood culture","volume":"130","author":"Randazzo","year":"2016","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.bjid.2019.05.005_bib0285","first-page":"379","article-title":"Turnaround time of positive blood cultures after the introduction of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry","volume":"38","author":"Angeletti","year":"2015","journal-title":"New Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0290","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1128\/CMR.18.4.638-656.2005","article-title":"Antimicrobial stewardship programs in health care systems","volume":"18","author":"MacDougall","year":"2005","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.bjid.2019.05.005_bib0295","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1002\/j.1875-9114.2012.01137.x","article-title":"Using rapid diagnostic tests to optimize antimicrobial selection in antimicrobial stewardship programs","volume":"32","author":"Goff","year":"2012","journal-title":"Pharmacotherapy"},{"key":"10.1016\/j.bjid.2019.05.005_bib0300","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1186\/s12879-016-1792-8","article-title":"Clinical relevance of molecular identification of microorganisms and detection of antimicrobial resistance genes in bloodstream infections of paediatric cancer patients","volume":"16","author":"Carlesse","year":"2016","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0305","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1093\/cid\/cis1204","article-title":"Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study","volume":"56","author":"Clerc","year":"2013","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0310","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1128\/AAC.04259-14","article-title":"Rapid testing using the Verigene Gram-negative blood culture nucleic acid test in combination with antimicrobial stewardship intervention against Gram-negative bacteremia","volume":"59","author":"Bork","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.bjid.2019.05.005_bib0315","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1086\/664910","article-title":"The economics of antimicrobial stewardship: the current state of the art and applying the business case model","volume":"33","author":"Stevenson","year":"2012","journal-title":"Infect Control Hosp Epidemiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0320","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1128\/JCM.01452-16","article-title":"Cost analysis of implementing matrix-assisted laser desorption ionization-time of flight mass spectrometry plus real-time antimicrobial stewardship intervention for bloodstream infections","volume":"55","author":"Patel","year":"2016","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0325","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.mimet.2014.12.009","article-title":"Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek\u00ae MS system","volume":"109","author":"Monteiro","year":"2015","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.bjid.2019.05.005_bib0330","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1128\/JCM.01831-09","article-title":"Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting","volume":"48","author":"Christner","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0335","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1128\/JCM.02485-09","article-title":"Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry","volume":"48","author":"Ferroni","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0340","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3343\/alm.2013.33.1.14","article-title":"Rapid clinical bacteriology and its future impact","volume":"33","author":"Van Belkum","year":"2013","journal-title":"Ann Lab Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0345","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.5858\/arpa.2012-0651-OA","article-title":"Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs","volume":"137","author":"Perez","year":"2013","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.bjid.2019.05.005_bib0350","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1099\/jmm.0.075580-0","article-title":"Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI Sepsityper combined with the BD Phoenix automated system","volume":"63","author":"Hazelton","year":"2014","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0355","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/JCM.06169-11","article-title":"Clinical impact of a real-time PCR assay for rapid identification of staphylococcal bacteremia","volume":"50","author":"Frye","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0360","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1111\/1469-0691.12640","article-title":"Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium","volume":"20","author":"Idelevich","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.bjid.2019.05.005_bib0365","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1128\/JCM.00529-14","article-title":"Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures","volume":"52","author":"Martinez","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0370","doi-asserted-by":"crossref","first-page":"827416","DOI":"10.1155\/2015\/827416","article-title":"Rapid identification of pathogens in positive blood culture of patients with sepsis: review and meta-analysis of the performance of the Sepsityper kit","volume":"2015","author":"Morgenthaler","year":"2015","journal-title":"Int J Microbiol"},{"key":"10.1016\/j.bjid.2019.05.005_bib0375","doi-asserted-by":"crossref","first-page":"32589","DOI":"10.1371\/journal.pone.0032589","article-title":"Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia","volume":"7","author":"Vlek","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bjid.2019.05.005_bib0380","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1093\/cid\/ciu039","article-title":"Introduction of rapid methicillin-resistant Staphylococcus aureus polymerase chain reaction testing and antibiotic selection among hospitalized patients with purulent skin infections","volume":"58","author":"Terp","year":"2014","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.bjid.2019.05.005_bib0385","doi-asserted-by":"crossref","first-page":"637","DOI":"10.2147\/TCRM.S2838","article-title":"Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time","volume":"4","author":"Ly","year":"2008","journal-title":"Ther Clin Risk Manag"},{"key":"10.1016\/j.bjid.2019.05.005_bib0390","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/1469-0691.12282","article-title":"Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry on patient management","volume":"19","author":"Martiny","year":"2013","journal-title":"Clin Microbiol Infect"}],"container-title":["The Brazilian Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1413867018310742?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1413867018310742?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T15:54:13Z","timestamp":1761407653000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1413867018310742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["S1413867018310742"],"URL":"https:\/\/doi.org\/10.1016\/j.bjid.2019.05.005","relation":{},"ISSN":["1413-8670"],"issn-type":[{"type":"print","value":"1413-8670"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach","name":"articletitle","label":"Article Title"},{"value":"The Brazilian Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bjid.2019.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Sociedade Brasileira de Infectologia. Published by Elsevier Espa\u00f1a, S.L.U.","name":"copyright","label":"Copyright"}]}}