{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:20:14Z","timestamp":1771262414081,"version":"3.50.1"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Brazilian Journal of Microbiology"],"published-print":{"date-parts":[[2018,11]]},"DOI":"10.1016\/j.bjm.2018.04.003","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T10:14:58Z","timestamp":1524564898000},"page":"220-223","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"S1","title":["A high prevalence of human papillomavirus 16 and 18 co-infections in cervical biopsies from southern Brazil"],"prefix":"10.1007","volume":"49","author":[{"given":"Sheile Pinheiro de","family":"Jesus","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carla Marques da","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina Bones","family":"Barcellos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubia Mar\u00edlia de","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Maria Dornelles da","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lucia","family":"Rossetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"issue":"5","key":"10.1016\/j.bjm.2018.04.003_bib0140","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int J Cancer"},{"key":"10.1016\/j.bjm.2018.04.003_bib0145","unstructured":"GLOBOCAN \u2013 Brazil; 2012. Available from: http:\/\/globocan.iarc.fr\/Pages\/fact_sheets_population.aspx [homepage on the Internet] [updated 2012. Accessed 29.08.17]."},{"key":"10.1016\/j.bjm.2018.04.003_bib0150","unstructured":"INCA \u2013 Instituto Nacional do Cancer; 2016. Available from: http:\/\/www.inca.gov.br\/estimativa\/2016\/mapa.asp?ID=5 [homepage on the Internet] [updated 2016; Accessed 29.08.17]."},{"key":"10.1016\/j.bjm.2018.04.003_bib0155","unstructured":"IARC \u2013 International Agency for Research on Cancer; 2015. Available from: http:\/\/monographs.iarc.fr\/ENG\/Classification\/List_of_Classifications.pdf [homepage on the Internet] [updated July 2017; Accessed 29.08.17]."},{"issue":"4","key":"10.1016\/j.bjm.2018.04.003_bib0160","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1136\/jcp.55.4.244","article-title":"The causal relation between human papillomavirus and cervical cancer","volume":"55","author":"Bosch","year":"2002","journal-title":"J Clin Pathol"},{"key":"10.1016\/j.bjm.2018.04.003_bib0165","unstructured":"ICO \u2013 Institut Catal\u00e0 d\u2019Oncologia on HPV and Cervical Cancer (HPV Information Centre). Summary report on HPV and cervical cancer statistics in Brazil; 2017. Available from: http:\/\/www.hpvcentre.net\/statistics\/reports\/BRA_FS.pdf [homepage on the Internet] [updated 2017; Accessed 29.08.17]."},{"issue":"5","key":"10.1016\/j.bjm.2018.04.003_bib0170","doi-asserted-by":"crossref","first-page":"e5060","DOI":"10.1590\/1414-431X20155060","article-title":"HPV vaccines: a controversial issue?","volume":"49","author":"Nicol","year":"2016","journal-title":"Braz J Med Biol Res"},{"issue":"2","key":"10.1016\/j.bjm.2018.04.003_bib0175","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1590\/S1676-24442007000200008","article-title":"Evaluation of two methods of DNA extraction from paraffin-embedded material for PCR amplification","volume":"43","author":"Simonato","year":"2007","journal-title":"J Bras Patol Med Lab"},{"issue":"1\/2","key":"10.1016\/j.bjm.2018.04.003_bib0180","first-page":"143","article-title":"Extra\u00e7\u00e3o e purifica\u00e7\u00e3o de DNA em material Biol\u00f3gico parafinado","volume":"35","author":"Vilanova-Costa","year":"2008","journal-title":"Estudos, Goi\u00e2nia"},{"key":"10.1016\/j.bjm.2018.04.003_bib0185","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1099\/0022-1317-76-4-1057","article-title":"The use of general primers GP5 and GP6 elongated at their 3\u2032 ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR","volume":"76","author":"De Roda Husman","year":"1995","journal-title":"J Gen Virol"},{"issue":"4732","key":"10.1016\/j.bjm.2018.04.003_bib0190","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1126\/science.2999980","article-title":"Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia","volume":"230","author":"Saiki","year":"1985","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.bjm.2018.04.003_bib0195","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jviromet.2011.06.010","article-title":"Evaluation of a novel microplate colorimetric hybridization genotyping assay for human papillomavirus","volume":"177","author":"Barcellos","year":"2011","journal-title":"J Virol Methods"},{"issue":"1","key":"10.1016\/j.bjm.2018.04.003_bib0200","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/jmv.20512","article-title":"Detection of specific human papillomavirus types in paraffin-embedded sections of cervical carcinomas","volume":"78","author":"Bryan","year":"2006","journal-title":"J Med Virol"},{"key":"10.1016\/j.bjm.2018.04.003_bib0205","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12985-014-0234-8","article-title":"Identification of human papillomaviruses from formalin-fixed, paraffin-embedded pre-cancer and invasive cervical cancer specimens in Zambia: a cross-sectional study","volume":"12","author":"Bateman","year":"2015","journal-title":"Virol J"},{"issue":"2","key":"10.1016\/j.bjm.2018.04.003_bib0210","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1002\/jmv.23437","article-title":"Genotyping of human papillomavirus in paraffin embedded cervical tissue samples from women in Ethiopia and the Sudan","volume":"85","author":"Abate","year":"2013","journal-title":"J Med Virol"},{"key":"10.1016\/j.bjm.2018.04.003_bib0215","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijid.2015.02.018","article-title":"Prevalence of high risk Human Papillomavirus in cervical dysplasia and cancer samples from twin cities in Pakistan","volume":"34","author":"Gul","year":"2015","journal-title":"Int J Infect Dis"},{"issue":"2","key":"10.1016\/j.bjm.2018.04.003_bib0220","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1590\/S0074-02762003000200003","article-title":"Human papillomavirus prevalence among women with cervical intraepithelial neoplasia III and invasive cervical cancer from Goi\u00e2nia, Brazil","volume":"98","author":"Rabelo-Santos","year":"2003","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"4","key":"10.1016\/j.bjm.2018.04.003_bib0225","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1002\/ijc.25396","article-title":"Human papillomavirus type distribution in 30,848 invasive cervical cancers worldwide: variation by geographical region, histological type and year of publication","volume":"128","author":"Li","year":"2011","journal-title":"Int J Cancer"},{"issue":"5","key":"10.1016\/j.bjm.2018.04.003_bib0230","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1128\/JCM.00087-13","article-title":"Multiple human papillomavirus infections with high viral loads are associated with cervical lesions but do not differentiate grades of cervical abnormalities","volume":"51","author":"Schmitt","year":"2013","journal-title":"J Clin Microbiol"},{"issue":"11","key":"10.1016\/j.bjm.2018.04.003_bib0235","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/dc.23143","article-title":"Genotypes and prevalence of HPV single and multiple concurrent infections in women with HSIL","volume":"42","author":"Beca","year":"2014","journal-title":"Diagn Cytopathol"},{"issue":"7","key":"10.1016\/j.bjm.2018.04.003_bib0240","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1093\/infdis\/jiq139","article-title":"Human papillomavirus infection with multiple types: pattern of coinfection and risk of cervical disease","volume":"203","author":"Chaturvedi","year":"2011","journal-title":"J Infect Dis"},{"key":"10.1016\/j.bjm.2018.04.003_bib0245","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1186\/1471-2334-14-214","article-title":"A portrait of single and multiple HPV type infections in Brazilian women of different age strata with squamous or glandular cervical lesions","volume":"14","author":"Resende","year":"2014","journal-title":"BMC Infect Dis"},{"issue":"9","key":"10.1016\/j.bjm.2018.04.003_bib0250","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1086\/507909","article-title":"Atypical squamous cells of undetermined significance\/low-Grade squamous intraepithelial lesions triage study group Human Papillomavirus genotypes and the cumulative 2-year risk of cervical precancer","volume":"194","author":"Wheeler","year":"2006","journal-title":"J Infect Dis"},{"issue":"7","key":"10.1016\/j.bjm.2018.04.003_bib0255","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.3390\/v6072762","article-title":"Prevalence and genotyping of high risk human papillomavirus in cervical cancer samples from Punjab, Pakistan","volume":"6","author":"Siddiqa","year":"2014","journal-title":"Viruses"},{"key":"10.1016\/j.bjm.2018.04.003_bib0260","unstructured":"Atlas On-line de Mortalidade. Available from: https:\/\/mortalidade.inca.gov.br\/MortalidadeWeb\/pages\/Modelo08\/consultar.xhtml [homepage on the Internet] [Accessed 12.03.18]."},{"key":"10.1016\/j.bjm.2018.04.003_bib0265","unstructured":"ICO\/IARC Information Centre on HPV and Cancer. Disease burden estimates \u2013 cervical cancer \u2013 mortality rate. Available from: http:\/\/www.hpvcentre.net\/parser.php?xml=M2_Cervical Cancer_Mortality Rates&iso=XWX&title=M2 [homepage on the Internet] [updated Nov 2015; Accessed 12.03.18]."},{"issue":"1","key":"10.1016\/j.bjm.2018.04.003_bib0270","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0102-311X2005000100033","article-title":"Evolution of uterine cervical cancer mortality from 1979 to 1998 in the State of Rio Grande do Sul, Brazil","volume":"21","author":"Kalakun","year":"2005","journal-title":"Cad Saude Publica"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1517838217308328?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1517838217308328?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T07:08:35Z","timestamp":1546412915000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1517838217308328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":27,"alternative-id":["S1517838217308328"],"URL":"https:\/\/doi.org\/10.1016\/j.bjm.2018.04.003","relation":{},"ISSN":["1517-8382"],"issn-type":[{"value":"1517-8382","type":"print"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A high prevalence of human papillomavirus 16 and 18 co-infections in cervical biopsies from southern Brazil","name":"articletitle","label":"Article Title"},{"value":"Brazilian Journal of Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bjm.2018.04.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.","name":"copyright","label":"Copyright"}]}}