{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:59:32Z","timestamp":1790423972247,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100009360","name":"King\u2019s College London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009360","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Norwich Research Park"},{"name":"Japanese Patent Office"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic &amp; Medicinal Chemistry"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.bmc.2017.04.006","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T23:31:12Z","timestamp":1491867072000},"page":"3182-3194","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"title":["Covalent inhibitors of LgtC: A blueprint for the discovery of non-substrate-like inhibitors for bacterial glycosyltransferases"],"prefix":"10.1016","volume":"25","author":[{"given":"Yong","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirella","family":"Vivoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Ema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niina","family":"Goos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Gehrke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Harmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd K.","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bmc.2017.04.006_b0005","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.sbi.2014.08.012","article-title":"Advances in understanding glycosyltransferases from a structural perspective","volume":"28","author":"Gloster","year":"2014","journal-title":"Curr Opin Struct Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0010","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1126\/science.aad1172","article-title":"Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation","volume":"351","author":"Petrou","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.bmc.2017.04.006_b0015","first-page":"E1190","article-title":"Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein","volume":"113","author":"Chen","year":"2016","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.bmc.2017.04.006_b0020","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nchembio.1029","article-title":"Developing inhibitors of glycan processing enzymes as tools for enabling glycobiology","volume":"8","author":"Gloster","year":"2012","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0025","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1039\/9781849737173-00078","article-title":"Recent design of glycosyltransferase inhibitors","volume":"39","author":"Wang","year":"2013","journal-title":"Carbohydr Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0030","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/nchembio.999","article-title":"Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome","volume":"8","author":"Rillahan","year":"2012","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0035","doi-asserted-by":"crossref","first-page":"40021","DOI":"10.1074\/jbc.M112.403568","article-title":"Metabolic inhibition of sialyl-lewis X biosynthesis by 5-thiofucose remodels the cell surface and impairs selectin-mediated cell adhesion","volume":"287","author":"Zandberg","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0040","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/nchembio.520","article-title":"Hijacking a biosynthetic pathway yields a glycosyltransferase inhibitor within cells","volume":"7","author":"Gloster","year":"2011","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0045","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1039\/C4MD00086B","article-title":"Beyond substrate analogues: new inhibitor chemotypes for glycosyltransferases","volume":"5","author":"Tedaldi","year":"2014","journal-title":"MedChemComm"},{"key":"10.1016\/j.bmc.2017.04.006_b0050","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1021\/acschembio.5b00004","article-title":"A small molecule that inhibits OGT activity in cells","volume":"10","author":"Ortiz-Meoz","year":"2015","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0055","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/nchembio.711","article-title":"A neutral diphosphate mimic crosslinks the active site of human O-GlcNAc transferase","volume":"8","author":"Jiang","year":"2012","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0060","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.chembiol.2012.12.006","article-title":"Developing irreversible inhibitors of the protein kinase cysteinome","volume":"20","author":"Liu","year":"2013","journal-title":"Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0065","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/nrd3410","article-title":"The resurgence of covalent drugs","volume":"10","author":"Singh","year":"2011","journal-title":"Nat Rev Drug Discovery"},{"key":"10.1016\/j.bmc.2017.04.006_b0070","doi-asserted-by":"crossref","first-page":"7590","DOI":"10.1021\/jm5006918","article-title":"Promiscuity and selectivity in covalent enzyme inhibition: a systematic study of electrophilic fragments","volume":"57","author":"Jost","year":"2014","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0075","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nchembio.925","article-title":"Reversible targeting of noncatalytic cysteines with chemically tuned electrophiles","volume":"8","author":"Serafimova","year":"2012","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0080","doi-asserted-by":"crossref","first-page":"7665","DOI":"10.1073\/pnas.0502860102","article-title":"Irreversible inhibitors of the EGF receptor may circumvent acquired resistance to gefitinib","volume":"102","author":"Kwak","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.bmc.2017.04.006_b0085","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/nchembio.492","article-title":"Selective irreversible inhibition of a protease by targeting a non-catalytic cysteine","volume":"7","author":"Hagel","year":"2011","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0090","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/84168","article-title":"Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs","volume":"8","author":"Persson","year":"2001","journal-title":"Nat Struct Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0095","doi-asserted-by":"crossref","first-page":"6226","DOI":"10.1128\/IAI.00722-06","article-title":"Role of lgtC in resistance of nontypeable Haemophilus influenzae strain R2866 to human serum","volume":"74","author":"Erwin","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.bmc.2017.04.006_b0100","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1039\/C4MD00077C","article-title":"A practical glycosyltransferase assay for the identification of new inhibitor chemotypes","volume":"5","author":"Tedaldi","year":"2014","journal-title":"MedChemComm"},{"key":"10.1016\/j.bmc.2017.04.006_b0105","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1016\/j.bmcl.2008.05.117","article-title":"Discovery of new Gram-negative antivirulence drugs: structure and properties of novel E. coli WaaC inhibitors","volume":"18","author":"Moreau","year":"2008","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.bmc.2017.04.006_b0110","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.chembiol.2004.02.024","article-title":"Identification of selective inhibitors for the glycosyltransferase MurG via high-throughput screening","volume":"11","author":"Hu","year":"2004","journal-title":"Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0115","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1021\/jm901070c","article-title":"Quantitative analyses of aggregation, autofluorescence, and reactivity artifacts in a screen for inhibitors of a thiol protease","volume":"53","author":"Jadhav","year":"2010","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0120","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cbpa.2014.10.021","article-title":"Covalent protein modification: the current landscape of residue-specific electrophiles","volume":"24","author":"Shannon","year":"2015","journal-title":"Curr Opin Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0125","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/jcc.23136","article-title":"CovalentDock: automated covalent docking with parameterized covalent linkage energy estimation and molecular geometry constrains","volume":"34","author":"Ouyang","year":"2013","journal-title":"J Comput Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0130","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1021\/jm201154p","article-title":"Inhibition of galactosyltransferases by a novel class of donor analogues","volume":"55","author":"Descroix","year":"2012","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0135","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1021\/jm901137j","article-title":"New substructure filters for removal of pan assay interference compounds (PAINS) from screening libraries and for their exclusion in bioassays","volume":"53","author":"Baell","year":"2010","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0140","doi-asserted-by":"crossref","first-page":"D490","DOI":"10.1093\/nar\/gkt1178","article-title":"The carbohydrate-active enzymes database (CAZy) in 2013","volume":"42","author":"Lombard","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bmc.2017.04.006_b0145","doi-asserted-by":"crossref","first-page":"35922","DOI":"10.1074\/jbc.M111.261172","article-title":"Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni","volume":"286","author":"Lee","year":"2011","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0150","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1038\/nchembio.1666","article-title":"Covalent docking of large libraries for the discovery of chemical probes","volume":"10","author":"London","year":"2014","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.bmc.2017.04.006_b0155","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1128\/MMBR.00007-14","article-title":"The sweet tooth of bacteria: common themes in bacterial glycoconjugates","volume":"78","author":"Tytgat","year":"2014","journal-title":"Mol Microbiol Rev"},{"key":"10.1016\/j.bmc.2017.04.006_b0160","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1038\/nrmicro3232","article-title":"Targeting virulence: can we make evolution-proof drugs?","volume":"12","author":"Allen","year":"2014","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.bmc.2017.04.006_b0165","doi-asserted-by":"crossref","first-page":"4761","DOI":"10.1021\/jm5002937","article-title":"Discovery of pyrazolopyridones as a novel class of noncovalent DprE1 inhibitor with potent anti-mycobacterial activity","volume":"57","author":"Panda","year":"2014","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0170","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/j.bmcl.2006.12.020","article-title":"5-((1H-pyrazol-4-yl)methylene)-2-thioxothiazolidin-4-one inhibitors of ADAMTS-5","volume":"17","author":"Gilbert","year":"2007","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.bmc.2017.04.006_b0175","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/glycob\/cwq187","article-title":"Universal phosphatase-coupled glycosyltransferase assay","volume":"21","author":"Wu","year":"2011","journal-title":"Glycobiology"},{"key":"10.1016\/j.bmc.2017.04.006_b0180","unstructured":"<http:\/\/www.genomics.agilent.com\/primerDesignProgram.jsp>."},{"key":"10.1016\/j.bmc.2017.04.006_b0185","unstructured":"MarvinSketch (version 5.12.4) for Mac OS X 10.7.5; <www.chemaxon.com>."},{"key":"10.1016\/j.bmc.2017.04.006_b0190","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera \u2013 a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J Comput Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0195","unstructured":"The PyMOL Molecular Graphics System, Version 1.7.4 Schr\u00f6dinger LLC."},{"key":"10.1016\/j.bmc.2017.04.006_b0200","doi-asserted-by":"crossref","first-page":"17796","DOI":"10.1074\/jbc.M809804200","article-title":"The crystal structures of the open and catalytically competent closed conformation of Escherichia coli glycogen synthase","volume":"284","author":"Sheng","year":"2009","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0205","doi-asserted-by":"crossref","first-page":"8041","DOI":"10.1074\/jbc.M113.545384","article-title":"Structures of complexes of a metal-independent glycosyltransferase GT6 from Bacteroides ovatus with UDP-N-acetylgalactosamine (UDP-GalNAc) and its hydrolysis products","volume":"289","author":"Pham","year":"2014","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bmc.2017.04.006_b0210","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1107\/S0907444911053157","article-title":"Structures of NodZ \u03b1-1,6-fucosyltransferase in complex with GDP and GDP-fucose","volume":"68","author":"Brzezinski","year":"2012","journal-title":"Acta Crystallogr Sect D"},{"key":"10.1016\/j.bmc.2017.04.006_b0215","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nature11744","article-title":"Crystallographic snapshot of cellulose synthesis and membrane translocation","volume":"493","author":"Morgan","year":"2012","journal-title":"Nature"}],"container-title":["Bioorganic &amp; Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0968089617301797?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0968089617301797?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:49:45Z","timestamp":1760834985000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0968089617301797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["S0968089617301797"],"URL":"https:\/\/doi.org\/10.1016\/j.bmc.2017.04.006","relation":{},"ISSN":["0968-0896"],"issn-type":[{"value":"0968-0896","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Covalent inhibitors of LgtC: A blueprint for the discovery of non-substrate-like inhibitors for bacterial glycosyltransferases","name":"articletitle","label":"Article Title"},{"value":"Bioorganic & Medicinal Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bmc.2017.04.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}