{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:59:26Z","timestamp":1791323966091,"version":"4.3.1"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic & Medicinal Chemistry"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.bmc.2025.118266","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T21:44:06Z","timestamp":1749159846000},"page":"118266","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Design, synthesis, and biological evaluation of long-acting glucagon-like peptide-1 (GLP-1) conjugates modified with dual fatty acids and a proline-alanine-serine (PAS) polypeptide"],"prefix":"10.1016","volume":"128","author":[{"given":"Chengcheng","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhen","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyong","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3695-1209","authenticated-orcid":false,"given":"Jun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bmc.2025.118266_b0005","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.peptides.2017.12.021","article-title":"Gut hormone polyagonists for the treatment of type 2 diabetes","volume":"100","author":"Brandt","year":"2018","journal-title":"Peptides"},{"key":"10.1016\/j.bmc.2025.118266_b0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.diabres.2021.109118","article-title":"IDF diabetes atlas: global estimates of undiagnosed diabetes in adults for 2021","volume":"183","author":"Ogurtsova","year":"2022","journal-title":"Diabetes Res Clin Pract"},{"key":"10.1016\/j.bmc.2025.118266_b0015","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/s41574-022-00690-7","article-title":"The burden and risks of emerging complications of diabetes mellitus","volume":"18","author":"Tomic","year":"2022","journal-title":"Nat Rev Endocrinol"},{"key":"10.1016\/j.bmc.2025.118266_b0020","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1111\/dom.13846","article-title":"Evaluation of weight change and hypoglycaemia as mediators in the association between insulin use and death","volume":"21","author":"Min","year":"2019","journal-title":"Diabetes Obes Metab"},{"key":"10.1016\/j.bmc.2025.118266_b0025","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s40364-025-00765-3","article-title":"Glucagon-like peptide 1 receptor agonists and cancer risk: advancing precision medicine through mechanistic understanding and clinical evidence","volume":"13","author":"Lin","year":"2025","journal-title":"Biomarker Res"},{"key":"10.1016\/j.bmc.2025.118266_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2024.116342","article-title":"Glucagon-like peptide-1 analogs: Miracle drugs are blooming?","volume":"269","author":"Gong","year":"2024","journal-title":"Eur J Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0035","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/00325481.2021.2002616","article-title":"Managing the gastrointestinal side effects of GLP-1 receptor agonists in obesity: recommendations for clinical practice","volume":"134","author":"Wharton","year":"2022","journal-title":"Postgrad Med"},{"key":"10.1016\/j.bmc.2025.118266_b0040","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1111\/dom.14058","article-title":"A glycosylated Fc-fused glucagon-like peptide-1 receptor agonist exhibits equivalent glucose lowering to but fewer gastrointestinal side effects than dulaglutide","volume":"22","author":"An","year":"2020","journal-title":"Diabetes Obes Metab"},{"key":"10.1016\/j.bmc.2025.118266_b0045","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1021\/bc049735+","article-title":"Synthesis, characterization, and pharmacokinetic studies of PEGylated glucagon-like peptide-1","volume":"16","author":"Lee","year":"2005","journal-title":"Bioconjug Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0050","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1021\/jm9909645","article-title":"Potent derivatives of glucagon-like Peptide-1 with Pharmacokinetic properties suitable for once daily Administration","volume":"43","author":"Knudsen","year":"2000","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0055","doi-asserted-by":"crossref","first-page":"E352","DOI":"10.1152\/ajpendo.2000.278.2.E352","article-title":"Measurement of interstitial albumin in human skeletal muscle and adipose tissue by open-flow microperfusion","volume":"278","author":"Ellmerer","year":"2000","journal-title":"Am J Physiol-Endocrinol Metab"},{"key":"10.1016\/j.bmc.2025.118266_b0060","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/s41573-022-00529-w","article-title":"Derivatization with fatty acids in peptide and protein drug discovery","volume":"22","author":"Kurtzhals","year":"2023","journal-title":"Nat Rev Drug Discov"},{"key":"10.1016\/j.bmc.2025.118266_b0065","doi-asserted-by":"crossref","first-page":"7370","DOI":"10.1021\/acs.jmedchem.5b00726","article-title":"Discovery of the once-weekly glucagon-like Peptide-1 (GLP-1) analogue Semaglutide","volume":"58","author":"Lau","year":"2015","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.bmc.2023.117291","article-title":"Design, synthesis, and biological evaluation of a potential long-acting glucagon-like peptide-1 (GLP-1) analog","volume":"85","author":"Zhang","year":"2023","journal-title":"Bioorg Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0075","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/protein\/gzt023","article-title":"PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins","volume":"26","author":"Schlapschy","year":"2013","journal-title":"Protein Eng Des Sel"},{"key":"10.1016\/j.bmc.2025.118266_b0080","doi-asserted-by":"crossref","first-page":"6126","DOI":"10.1021\/jm070861j","article-title":"Structure\u2212activity and protraction relationship of long-acting glucagon-like Peptide-1 derivatives: importance of fatty acid length, Polarity, and Bulkiness","volume":"50","author":"Madsen","year":"2007","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0085","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1021\/acs.bioconjchem.3c00464","article-title":"Long-term strategies for poorly water-soluble peptides: combining fatty acid modification with PAS Fusion","volume":"34","author":"Zhu","year":"2023","journal-title":"Bioconjug Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.bmc.2021.116291","article-title":"Design, synthesis and biological evaluation of double fatty chain-modified glucagon-like peptide-1 conjugates","volume":"44","author":"Zhang","year":"2021","journal-title":"Bioorg Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0095","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nature22394","article-title":"Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein","volume":"546","author":"Zhang","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.bmc.2025.118266_b0100","doi-asserted-by":"crossref","first-page":"18039","DOI":"10.1038\/srep18039","article-title":"Variant fatty acid-like molecules conjugation, novel approaches for extending the stability of therapeutic peptides","volume":"5","author":"Li","year":"2015","journal-title":"Sci Rep"},{"key":"10.1016\/j.bmc.2025.118266_b0105","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.diabet.2017.05.009","article-title":"Effect of GLP-1 receptor agonist on gastrointestinal tract motility and residue rates as evaluated by capsule endoscopy","volume":"43","author":"Nakatani","year":"2017","journal-title":"Diabetes Metab"},{"key":"10.1016\/j.bmc.2025.118266_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.peptides.2020.170342","article-title":"Intestinal glucagon-like peptide-1 effects on food intake: physiological relevance and emerging mechanisms","volume":"131","author":"Krieger","year":"2020","journal-title":"Peptides"},{"key":"10.1016\/j.bmc.2025.118266_b0115","doi-asserted-by":"crossref","first-page":"639","DOI":"10.3390\/applbiosci2040040","article-title":"Unlocking insights into folding, structure, and function of proteins through Circular dichroism spectroscopy\u2014A short Review","volume":"2","author":"Linhares","year":"2023","journal-title":"Appl Biosci"},{"key":"10.1016\/j.bmc.2025.118266_b0120","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1021\/mp5007147","article-title":"PASylation of murine leptin leads to extended plasma half-life and enhanced in vivo Efficacy","volume":"12","author":"Morath","year":"2015","journal-title":"Mol Pharm"},{"key":"10.1016\/j.bmc.2025.118266_b0125","doi-asserted-by":"crossref","DOI":"10.1021\/acs.jmedchem.8b00560","article-title":"New analogs of the complement C3 inhibitor compstatin with increased solubility and improved Pharmacokinetic Profile","author":"Berger","year":"2018","journal-title":"J Med Chem"},{"key":"10.1016\/j.bmc.2025.118266_b0130","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1038\/nbt1094-1017","article-title":"A novel approach for high level production of a recombinant human Parathyroid hormone fragment in Escherichia coli","volume":"12","author":"Gram","year":"1994","journal-title":"Bio\/Technology"}],"container-title":["Bioorganic & Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096808962500207X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096808962500207X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T02:39:00Z","timestamp":1775097540000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096808962500207X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":26,"alternative-id":["S096808962500207X"],"URL":"https:\/\/doi.org\/10.1016\/j.bmc.2025.118266","relation":{},"ISSN":["0968-0896"],"issn-type":[{"value":"0968-0896","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Design, synthesis, and biological evaluation of long-acting glucagon-like peptide-1 (GLP-1) conjugates modified with dual fatty acids and a proline-alanine-serine (PAS) polypeptide","name":"articletitle","label":"Article Title"},{"value":"Bioorganic & Medicinal Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bmc.2025.118266","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"118266"}}