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Neurol."},{"key":"10.1016\/j.bmcl.2005.05.006_bib11","doi-asserted-by":"crossref","first-page":"9675","DOI":"10.1016\/S0040-4020(00)00922-4","volume":"56","author":"Kimbaris","year":"2000","journal-title":"Tetrahedron"},{"key":"10.1016\/j.bmcl.2005.05.006_bib12_1","unstructured":"Data for 7: as an oil; IR (Neat) \u03bdmax 3388, 3097, 3068, 2915, 2105, 1597, 1578cm\u22121; 1H NMR (CDCl3, 400MHz) \u03b4H (ppm) 4.06 (s, 2H, CH2), 6.04 (m, 1H, H-3), 6.19 (m, 1H, H-4), 6.73 (m, 1H, H-5), 7.14\u20137.26 (m, 4H, H-3\u2032, H-4\u2032, H-5\u2032, H-6\u2032), 7.99 (br s, 1H, NH pyrrole); 13C NMR (CDCl3, 100MHz) \u03b4C (ppm) 31.26, 107.75, 109.39, 118.03, 127.40, 128.37, 128.43, 129.07, 130.65, 130.77, 135.20, 137.02; MS (EI), m\/z 214 (M+, 100), 186 (28), 181 (31), 154 (12), 80 (8); EI HRMS M+, found 214.0559 C12H10N2S requires 214.0565"},{"key":"10.1016\/j.bmcl.2005.05.006_bib12_2","unstructured":"Data for 8: as a light yellow powder mp=183\u00b0C (dec); IR (Nujol) \u03bdmax 3165, 3111, 1609, 1583cm\u22121; 1H NMR (CDCl3, 400MHz) \u03b4H (ppm) 3.95 (s, 2H, CH2), 6.03 (m, 1H, H-1), 6.20 (t, 1H, J=3.3Hz, H-2), 7.15 (m, 1H, H-10), 7.20\u20137.29 (m, 3H, H-7, H-8, H-9), 7.68 (dd, 1H, J=3.4, 1.9Hz, H-3), 9.91 (br s, 1H, NH); 13C NMR (CDCl3, 100MHz) \u03b4C (ppm) 31.57, 109.32, 112.26, 120.88, 125.61, 127.28, 127.53, 128.03, 132.58, 132.89, 136.40, 180.17; MS (EI), m\/z 214 (M+, 100), 186 (33), 181 (32), 149 (36), 135 (20), 97 (15%); EI HRMS M+, found 214.0556 C12H10N2S requires 214.0565"},{"key":"10.1016\/j.bmcl.2005.05.006_bib12_3","unstructured":"Compounds 4\u20136 were characterized by satisfactory elemental analyses, and from their mass, 1H and 13C NMR spectra"},{"key":"10.1016\/j.bmcl.2005.05.006_bib13","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1152\/physrev.2000.80.1.315","volume":"80","author":"Nichols","year":"2000","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bmcl.2005.05.006_bib14_1","unstructured":"Cerebrocortical cultures\u2014Cortical cultures of mixed neurons and glia were derived from embryonic (fetal day 15 or 16) Wistar rats. Briefly, following dissociation in 0.027% trypsin, cerebral cortical cells were plated either on 96-well multiwell plates or 35mm dishes that had been previously coated overnight with 15\u03bcg\/ml poly-L-lysine and then with DMEM (Sigma) culture medium supplemented with 10% fetal bovine serum. After removal of the final coating solution, cells were seeded (106ml) in a serum free medium composed of a mixture of DMEM, contained 40\u03bcg\/ml gentamycin, and 60\u03bcg\/ml penicillin. Cells were cultured at 37\u00b0C in a humidified atmosphere of 95% air and 5% CO2 and were used after 6\u20137 days"},{"key":"10.1016\/j.bmcl.2005.05.006_bib14_2","unstructured":"Jurkat cell culture\u2014The human Jurkat T cell line (clone E6-1) was obtained from the American Type Culture Collection (Manassas, VA). Cells were maintained in RPMI 1640 medium (Sigma) supplemented with 10% FBS (Sigma), 2mM L-glutamine (Life Technologies), and 10\u03bcg\/ml gentamycin (Life Technologies). Cells were grown at 37\u00b0C in a humidified atmosphere consisting of 5% CO2. Cells were passaged three times weekly and maintained at a density between 0.2 and 1\u00d7106cells\/ml. Cells used for all experiments were in logarithmic growth phase, and the medium used for experiments had the same constituents as that used for cell passage, unless otherwise indicated"},{"key":"10.1016\/j.bmcl.2005.05.006_bib14_3","unstructured":"MTT reduction cell viability assay\u2014Experiments were performed using glia\/neurons and Jurkat cells cultured in 96-well plates. The effects of heterocycles 2, 3, 5, 6 and 8 (1\u03bcM) or\/and NMDA (10\u03bcM) on cell viability was assessed by a colorimetric assay based on the cleavage of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) into a blue-colored formazan product by mitochondrial succinate dehydrogenase. Additions were made directly to the culture medium for 24h. Cells then washed twice with HEPES-buffered incubation medium (HBM) (140mM NaCl, 5mM KCl, 5mM NaHCO3, 1.1mM MgCl2, 1.2 CaCl2, 5.5mM glucose, and 20mM HEPES, pH7.4.) and incubated for 45min at 37\u00b0C in HBM containing MTT (0.5mg\/ml). After this period, the HBM was carefully removed, and the blue formazan product was solubilized in 300\u03bcl of 100% dimethyl sulfoxide. The absorbance of each well was read at 570nm. The data were treated by one-way ANOVA analysis. The data from each experiment were analyzed separately. Where a significant effect was observed in the ANOVA analysis, comparisons of those samples were made by the t-test"},{"key":"10.1016\/j.bmcl.2005.05.006_bib15","doi-asserted-by":"crossref","first-page":"133","DOI":"10.4049\/jimmunol.133.1.133","volume":"133","author":"Mohagheghpour","year":"1984","journal-title":"J. Immunol."},{"key":"10.1016\/j.bmcl.2005.05.006_bib17","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1021\/jm001064n","volume":"44","author":"Gregson","year":"2001","journal-title":"J. Med. 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