{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:33:05Z","timestamp":1774917185729,"version":"3.50.1"},"reference-count":43,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic & Medicinal Chemistry Letters"],"published-print":{"date-parts":[[2008,1]]},"DOI":"10.1016\/j.bmcl.2007.10.110","type":"journal-article","created":{"date-parts":[[2007,11,20]],"date-time":"2007-11-20T07:21:08Z","timestamp":1195543268000},"page":"152-158","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"title":["Carbonic anhydrase inhibitors. Interaction of 2-(hydrazinocarbonyl)-3-phenyl-1H-indole-5-sulfonamide with 12 mammalian isoforms: Kinetic and X-ray crystallographic studies"],"prefix":"10.1016","volume":"18","author":[{"given":"\u00d6zlen","family":"G\u00fczel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Temperini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessio","family":"Innocenti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Scozzafava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayd\u0131n","family":"Salman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bmcl.2007.10.110_bib1_1","series-title":"Carbonic Anhydrase\u2014Its Inhibitors and Activators","first-page":"1","year":"2004"},{"key":"10.1016\/j.bmcl.2007.10.110_bib1_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1517\/13543776.10.5.575","volume":"10","author":"Supuran","year":"2000","journal-title":"Expert Opin. Ther. Pat."},{"key":"10.1016\/j.bmcl.2007.10.110_bib1_3","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1517\/13543776.16.12.1627","volume":"16","author":"Scozzafava","year":"2006","journal-title":"Expert Opin. Ther. Pat."},{"key":"10.1016\/j.bmcl.2007.10.110_bib2_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1574-6976.2000.tb00546.x","volume":"24","author":"Smith","year":"2000","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.bmcl.2007.10.110_bib2_2","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1002\/med.10025","volume":"23","author":"Supuran","year":"2003","journal-title":"Med. Res. Rev."},{"key":"10.1016\/j.bmcl.2007.10.110_bib2_3","series-title":"Carbonic Anhydrase: Its Inhibitors and Activators","first-page":"25","article-title":"Acatalytic CAs: Carbonic Anhydrase-Related Proteins","author":"Nishimori","year":"2004"},{"key":"10.1016\/j.bmcl.2007.10.110_bib3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1042\/BJ20070816","volume":"407","author":"Ohradanova","year":"2007","journal-title":"Biochem. J."},{"key":"10.1016\/j.bmcl.2007.10.110_bib4","doi-asserted-by":"crossref","first-page":"7697","DOI":"10.1002\/anie.200701189","volume":"46","author":"K\u00f6hler","year":"2007","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bmcl.2007.10.110_bib5","doi-asserted-by":"crossref","first-page":"849","DOI":"10.2174\/156802607780636735","volume":"7","author":"Mincione","year":"2007","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib6_1","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1517\/13543776.13.10.1545","volume":"13","author":"Supuran","year":"2003","journal-title":"Expert Opin. Ther. Pat."},{"key":"10.1016\/j.bmcl.2007.10.110_bib6_2","doi-asserted-by":"crossref","first-page":"879","DOI":"10.2174\/156802607780636762","volume":"7","author":"De Simone","year":"2007","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib7","doi-asserted-by":"crossref","first-page":"855","DOI":"10.2174\/156802607780636726","volume":"7","author":"Thiry","year":"2007","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib8","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.tips.2006.09.002","volume":"27","author":"Thiry","year":"2006","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.bmcl.2007.10.110_bib9","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.febslet.2004.10.043","volume":"577","author":"\u0160vastov\u00e1","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bmcl.2007.10.110_bib10","doi-asserted-by":"crossref","first-page":"865","DOI":"10.2174\/156802607780636708","volume":"7","author":"Pastorekova","year":"2007","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib11","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.radonc.2007.04.018","volume":"83","author":"Dubois","year":"2007","journal-title":"Radiother. Oncol."},{"key":"10.1016\/j.bmcl.2007.10.110_bib12","doi-asserted-by":"crossref","first-page":"5544","DOI":"10.1021\/jm060531j","volume":"49","author":"De Simone","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib13","doi-asserted-by":"crossref","first-page":"909","DOI":"10.2174\/156802607780636744","volume":"7","author":"Krungkrai","year":"2007","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib14_1","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1021\/jm0512600","volume":"49","author":"Nishimori","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib14_2","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1016\/j.bmcl.2007.04.063","volume":"17","author":"Nishimori","year":"2007","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.bmcl.2007.10.110_bib15","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1016\/j.cub.2005.10.040","volume":"15","author":"Klengel","year":"2005","journal-title":"Curr. Biol."},{"key":"10.1016\/j.bmcl.2007.10.110_bib16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1128\/EC.5.1.103-111.2006","volume":"5","author":"Mogensen","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.bmcl.2007.10.110_bib17","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.mib.2006.09.003","volume":"9","author":"Bahn","year":"2006","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.bmcl.2007.10.110_bib18","doi-asserted-by":"crossref","first-page":"18782","DOI":"10.1074\/jbc.M414348200","volume":"280","author":"Suarez Covarrubias","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib19","series-title":"Carbonic Anhydrase\u2014Its Inhibitors and Activators","first-page":"67","article-title":"Development of Sulfonamide Carbonic Anhydrase Inhibitors","author":"Supuran","year":"2004"},{"key":"10.1016\/j.bmcl.2007.10.110_bib20","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1097\/wnf.0b013e3180413d7d","volume":"30","author":"Biton","year":"2007","journal-title":"Clin. Neuropharmacol."},{"key":"10.1016\/j.bmcl.2007.10.110_bib21","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1016\/j.bmcl.2005.03.032","volume":"15","author":"De Simone","year":"2005","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.bmcl.2007.10.110_bib22_1","doi-asserted-by":"crossref","first-page":"4834","DOI":"10.1021\/jm0501073","volume":"48","author":"Cecchi","year":"2005","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib22_2","doi-asserted-by":"crossref","first-page":"7024","DOI":"10.1021\/jm060807n","volume":"49","author":"Winum","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib22_3","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1021\/jm011112j","volume":"45","author":"Gr\u00fcneberg","year":"2002","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib23_1","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1021\/jm00013a004","volume":"38","author":"Boriack","year":"1995","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib23_2","doi-asserted-by":"crossref","first-page":"12125","DOI":"10.1021\/ja002627n","volume":"122","author":"Kim","year":"2000","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bmcl.2007.10.110_bib23_3","series-title":"Carbonic Anhydrase\u2014Its Inhibitors and Activators","first-page":"45","article-title":"Multiple Binding Modes Observed in X-ray Structures of Carbonic Anhydrase Inhibitor Complexes and Other Systems: Consequences for Structure-based Drug Design","author":"Antel","year":"2004"},{"key":"10.1016\/j.bmcl.2007.10.110_bib24","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1021\/jm010163d","volume":"45","author":"Kim","year":"2002","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib25","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1002\/pro.5560071201","volume":"7","author":"Boriack-Sjodin","year":"1998","journal-title":"Protein Sci."},{"key":"10.1016\/j.bmcl.2007.10.110_bib27","unstructured":"Khalifah, R. G. J. Biol. Chem. 1971, 246, 2561. An Applied Photophysics stopped-flow instrument has been used for assaying the CA catalysed CO2 hydration activity. Phenol red (at a concentration of 0.2mM) has been used as indicator, working at the absorbance maximum of 557nm, with 10mM Hepes (pH 7.5) as buffer, 0.1M Na2SO4 (for maintaining constant the ionic strength), following the CA-catalyzed CO2 hydration reaction for a period of 10\u2013100s. The CO2 concentrations ranged from 1.7 to 17mM for the determination of the kinetic parameters and inhibition constants. For each inhibitor at least six traces of the initial 5\u201310% of the reaction have been used for determining the initial velocity. The uncatalyzed rates were determined in the same manner and subtracted from the total observed rates. Stock solutions of inhibitor (0.1mM) were prepared in distilled-deionized water and dilutions up to 0.1nM were done thereafter with distilled-deionized water. Inhibitor and enzyme solutions were preincubated together for 15min at room temperature prior to assay, in order to allow for the formation of the E-I complex. The inhibition constants were obtained by non-linear least-squares methods using PRISM 3, as reported earlier,12\u201314 and represent the mean from at least three different determinations. Enzyme concentrations in the assay system were in the range of 7.1\u201313nM.12\u201314"},{"key":"10.1016\/j.bmcl.2007.10.110_bib28a","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1016\/j.bmc.2007.04.020","volume":"15","author":"Supuran","year":"2007","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib28b","doi-asserted-by":"crossref","first-page":"7229","DOI":"10.1016\/j.bmc.2007.08.037","volume":"15","author":"Nishimori","year":"2007","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.bmcl.2007.10.110_bib29","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nm1534","volume":"13","author":"Gao","year":"2007","journal-title":"Nat. Med."},{"key":"10.1016\/j.bmcl.2007.10.110_bib31","unstructured":"Oxford Diffraction. CrysAlis RED. 2006, Version 1.171.32.2. Oxford Diffraction Ltd, UK."},{"key":"10.1016\/j.bmcl.2007.10.110_bib32","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1107\/S0108767390010224","volume":"A47","author":"Jones","year":"1991","journal-title":"Acta Cryst."},{"key":"10.1016\/j.bmcl.2007.10.110_bib33","first-page":"2126","volume":"D60","author":"Emsley","year":"2004","journal-title":"Acta Cryst."},{"key":"10.1016\/j.bmcl.2007.10.110_bib34","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Crystallogr."}],"container-title":["Bioorganic & Medicinal Chemistry Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960894X07013030?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960894X07013030?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T05:51:12Z","timestamp":1760766672000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960894X07013030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["S0960894X07013030"],"URL":"https:\/\/doi.org\/10.1016\/j.bmcl.2007.10.110","relation":{},"ISSN":["0960-894X"],"issn-type":[{"value":"0960-894X","type":"print"}],"subject":[],"published":{"date-parts":[[2008,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Carbonic anhydrase inhibitors. Interaction of 2-(hydrazinocarbonyl)-3-phenyl-1H-indole-5-sulfonamide with 12 mammalian isoforms: Kinetic and X-ray crystallographic studies","name":"articletitle","label":"Article Title"},{"value":"Bioorganic & Medicinal Chemistry Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bmcl.2007.10.110","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}