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For each inhibitor at least six traces of the initial 5\u201310% of the reaction have been used for determining the initial velocity. The uncatalyzed rates were determined in the same manner and subtracted from the total observed rates. Stock solutions of inhibitor (0.1mM) were prepared in distilled\u2013deionized water and dilutions up to 0.01nM were done thereafter with the assay buffer. Inhibitor and enzyme solutions were preincubated together for 15min\u201312h at room temperature (15min) or 4\u00b0C (all other incubation times) prior to assay, in order to allow for the formation of the E\u2013I complex or for the eventual active site mediated hydrolysis of the inhibitor. Data reported in Table 1 show the inhibition after 12h incubation, which led to the eventual completion of the in situ hydrolysis of the heterocyclic ring, as reported earlier for couamrins.1b,2 The inhibition constants were obtained by non-linear least-squares methods using PRISM 3, as reported earlier,1b,2 and represent the mean from at least three different determinations. CA isofoms were recombinant ones obtained in house as reported earlier.1,2"},{"key":"10.1016\/j.bmcl.2012.03.071_b0085","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1021\/bi00704a015","volume":"13","author":"Pocker","year":"1974","journal-title":"Biochemistry"},{"key":"10.1016\/j.bmcl.2012.03.071_b0090","first-page":"7","volume":"1968","author":"Pocker","year":"1995","journal-title":"Biochemistry"},{"key":"10.1016\/j.bmcl.2012.03.071_b0095","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1021\/ja00087a086","volume":"116","author":"Nair","year":"1994","journal-title":"J. Am. Chem. 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