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Collect"},{"key":"10.1016\/j.bmcl.2020.127411_b0070","doi-asserted-by":"crossref","first-page":"710","DOI":"10.3390\/molecules22050710","volume":"22","author":"Al-Wahaibi","year":"2017","journal-title":"Molecules"},{"key":"10.1016\/j.bmcl.2020.127411_b0075","volume":"92","author":"Patila","year":"2019","journal-title":"Bioorg Chem"},{"key":"10.1016\/j.bmcl.2020.127411_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2019.111840","volume":"185","author":"Mazzotta","year":"2020","journal-title":"Eur J Med Chem"},{"key":"10.1016\/j.bmcl.2020.127411_b0085","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.ejmech.2012.10.042","volume":"58","author":"Vega-P\u00e9rez","year":"2012","journal-title":"Eur J Med Chem"},{"key":"10.1016\/j.bmcl.2020.127411_b0090","unstructured":"Synthesis of the thiourea derivatives (39-43). To a solution of the monoacyl derivative (0.75 mmol) in dry dichloromethane (10 mL) was added the corresponding isothiocyanate (0.9 mmol). The reaction mixture was stirred at room temperature until TLC showed that all the starting material had reacted, than was evaporated to dryness. Compounds were purified by flash chromatography on silica gel using the appropriate eluent."},{"key":"10.1016\/j.bmcl.2020.127411_b0095","unstructured":"Spectra data of the representative compound 41. 1H NMR (500 MHz, DMSO-d6) \u03b4 9.81 (s, 1H), 8.11 (s, 2H), 7.77 (s, 1H), 3.97 (t, J = 5.1 Hz, 4H), 3.48 (t, J = 5.1 Hz, 4H), 1.44 (s, 9H). 13C NMR (125 MHz, DMSO-d6) \u03b4 180.8, 153.8, 143.1, 130.1, 129.8, 129.6, 129.3, 129.2, 124.4, 124.3, 122.2, 116.6, 79.2, 47.8, 42.8, 28.0. HRMS (m\/z): calcd. for C18H21F6N3O2SNa 480.1151 [M+Na]+; found 480.1143. Anal. calcd for C18H21F6N3O2S: C, 47.26; H, 4.63; N, 9,19. Found: C, 46.95; H, 4.77; N, 9.17."},{"key":"10.1016\/j.bmcl.2020.127411_b0100","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1086\/595977","volume":"30","author":"Valencia","year":"2009","journal-title":"Infect Control Hosp Epidemiol"},{"key":"10.1016\/j.bmcl.2020.127411_b0105","unstructured":"The activity screening studies were performed as follows: All compounds were screened at 50 \u00b5M in 96-well plate to identify which produced inhibition of growth against 13 colistin-resistant A. baumannii clinical strains. For each strain, approximately 1x105 CFU\/mL bacteria in M\u00fcller Hinton Broth (MHB) were dispensed into the plates containing 50\u00b5M of each piperazine derivate. Plates were incubated at 37\u00b0C with humidity for 18-24 hours. The compounds were dissolved in DMSO, which was used as negative control. As a positive control, 1x105 CFU\/mL bacteria in broth, without compound was incubated in the same conditions. Growth was indicated by turbidity, a single large dot, or a filamentous network of growth. The experiments were performed in triplicate and were read independently by two observers."},{"key":"10.1016\/j.bmcl.2020.127411_b0110","unstructured":"Minimal inhibitory concentration (MIC) was determined by the broth microdilution method (cation-adjusted Mueller-Hinton broth; Becton Dickinson, Cockeysville, Md.), in accordance with the CLSI guidelines (Clinical and Laboratory Standards Institute, 2012).The compounds for which inhibition of the bacterial growth was observed in the screening studies were tested (6clinical strains). A range of piperazine derivates concentrations from 50\u00a0\u00b5M to 0.195\u00a0\u00b5M was tested. MICs were read as the lowest concentrations of piperazine derivate which inhibited visible growth after incubation for 24 h. The experiments were performed in triplicate and were read independently by two observers."},{"key":"10.1016\/j.bmcl.2020.127411_b0115","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/j.bmcl.2019.03.032","volume":"29","author":"Xu","year":"2019","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.bmcl.2020.127411_b0120","doi-asserted-by":"crossref","first-page":"27258","DOI":"10.1038\/srep27258","volume":"6","author":"Lyu","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.bmcl.2020.127411_b0125","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.bbrep.2016.03.014","volume":"6","author":"Cherdtrakulkiat","year":"2016","journal-title":"Biochem Biophys Reports"},{"key":"10.1016\/j.bmcl.2020.127411_b0130","doi-asserted-by":"crossref","first-page":"3682","DOI":"10.1002\/adfm.201202850","volume":"23","author":"Ong","year":"2013","journal-title":"Adv Funct Mater"},{"key":"10.1016\/j.bmcl.2020.127411_b0135","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4155\/fmc-2016-0186","volume":"9","author":"Carullo","year":"2017","journal-title":"Future Med Chem"},{"key":"10.1016\/j.bmcl.2020.127411_b0140","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1038\/s41591-019-0675-0","volume":"25","author":"Furman","year":"2019","journal-title":"Nat Med"},{"key":"10.1016\/j.bmcl.2020.127411_b0145","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/cbdd.12947","volume":"90","author":"Corr\u00eaa","year":"2017","journal-title":"Chem Biol Drug Des"},{"issue":"11","key":"10.1016\/j.bmcl.2020.127411_b0150","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.3390\/molecules21111544","volume":"21","author":"Li","year":"2016","journal-title":"Molecules"},{"issue":"137","key":"10.1016\/j.bmcl.2020.127411_b0155","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.pbb.2015.08.008","volume":"2015","author":"Silva","year":"2015","journal-title":"Pharmacol Biochem Behav"},{"issue":"12","key":"10.1016\/j.bmcl.2020.127411_b0160","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.3390\/molecules21121684","volume":"21","author":"Ma","year":"2016","journal-title":"Molecules"},{"key":"10.1016\/j.bmcl.2020.127411_b0165","doi-asserted-by":"crossref","first-page":"825","DOI":"10.3389\/fphar.2017.00825","volume":"8","author":"Corr\u00eaa","year":"2017","journal-title":"Front Pharmacol"},{"issue":"4","key":"10.1016\/j.bmcl.2020.127411_b0170","first-page":"1027","volume":"11","author":"Ahmadi","year":"2012","journal-title":"Iran J Pharmac Res: IJPR"},{"issue":"1","key":"10.1016\/j.bmcl.2020.127411_b0175","first-page":"84","volume":"62","author":"Chen","year":"2016","journal-title":"Cell Mol Biol"},{"key":"10.1016\/j.bmcl.2020.127411_b0180","first-page":"9","volume":"2018","author":"Thangam","year":"1873","journal-title":"Front Immunol"},{"key":"10.1016\/j.bmcl.2020.127411_b0185","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.ejmech.2015.07.026","volume":"102","author":"Shaquiquzzaman","year":"2015","journal-title":"Eur J Med Chem"},{"issue":"6","key":"10.1016\/j.bmcl.2020.127411_b0190","first-page":"1012","volume":"25","author":"Mohan","year":"2014","journal-title":"J Braz Chem Soc"},{"issue":"20","key":"10.1016\/j.bmcl.2020.127411_b0195","doi-asserted-by":"crossref","first-page":"6385","DOI":"10.1016\/j.bmcl.2012.08.071","volume":"22","author":"Hatnapure","year":"2012","journal-title":"Bioorganic & Med Chem Lett"},{"key":"10.1016\/j.bmcl.2020.127411_b0200","unstructured":"Inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. 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