{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:47:22Z","timestamp":1791388042224,"version":"4.3.3"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402442"],"award-info":[{"award-number":["32402442"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31402036"],"award-info":[{"award-number":["31402036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M760857"],"award-info":[{"award-number":["2024M760857"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017882","name":"Yellow Crane Talents Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017882","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic & Medicinal Chemistry Letters"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.bmcl.2025.130162","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T05:52:18Z","timestamp":1741240338000},"page":"130162","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Discovery of Flavonol derivatives as porcine reproductive and respiratory syndrome virus inhibitors"],"prefix":"10.1016","volume":"121","author":[{"given":"Zhi-Zheng","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Xu","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Hao","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.bmcl.2025.130162_bb0005","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.theriogenology.2006.04.024","article-title":"Porcine reproductive and respiratory syndrome virus","volume":"66","author":"Cho","year":"2006","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0010","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.virusres.2010.08.016","article-title":"Control and elimination of porcine reproductive and respiratory syndrome virus","volume":"154","author":"Corzo","year":"2010","journal-title":"Virus Res"},{"key":"10.1016\/j.bmcl.2025.130162_bb0015","first-page":"6","article-title":"Key gaps in the knowledge of the porcine respiratory reproductive syndrome virus (PRRSV). Frontiers in veterinary","author":"Montaner-Tarbes","year":"2019","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0020","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.virusres.2010.07.016","article-title":"Porcine reproductive and respiratory syndrome in China","volume":"154","author":"Zhou","year":"2010","journal-title":"Virus Res"},{"key":"10.1016\/j.bmcl.2025.130162_bb0025","first-page":"5","article-title":"Production losses from an endemic animal disease: porcine reproductive and respiratory syndrome (PRRS) in selected Midwest US sow farms. Frontiers in veterinary","author":"Valdes-Donoso","year":"2018","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.bmcl.2025.130162_bb0030","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tvjl.2007.05.022","article-title":"The challenge of PRRS immunology","volume":"177","author":"Mateu","year":"2008","journal-title":"Vet J"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0035","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.virusres.2010.09.003","article-title":"Taming PRRSV: revisiting the control strategies and vaccine design","volume":"154","author":"Thanawongnuwech","year":"2010","journal-title":"Virus Res"},{"key":"10.1016\/j.bmcl.2025.130162_bb0040","series-title":"Zhou E-M","first-page":"8","author":"Nan","year":"2017"},{"key":"10.1016\/j.bmcl.2025.130162_bb0045","first-page":"11","article-title":"Antiviral strategies of Chinese herbal medicine against PRRSV infection","author":"Gs","year":"2020","journal-title":"Front Microbiol"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0050","doi-asserted-by":"crossref","first-page":"4813","DOI":"10.1038\/s41467-024-48807-y","article-title":"High-throughput screening unveils nitazoxanide as a potent PRRSV inhibitor by targeting NMRAL1","volume":"15","author":"Cui","year":"2024","journal-title":"Nat Commun"},{"issue":"5","key":"10.1016\/j.bmcl.2025.130162_bb0055","doi-asserted-by":"crossref","first-page":"e00023","DOI":"10.1128\/jvi.00054-23","article-title":"Identification of new compounds against PRRSV infection by directly targeting CD163","volume":"97","author":"Zhu","year":"2023","journal-title":"J Virol"},{"issue":"9","key":"10.1016\/j.bmcl.2025.130162_bb0060","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.3390\/pharmaceutics14091793","article-title":"Flavonoids as potential antiviral agents for porcine viruses","volume":"14","author":"Zhang","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.bmcl.2025.130162_bb0065","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.antiviral.2018.07.012","article-title":"Multiple antiviral approaches of (\u2212)-epigallocatechin-3-gallate (EGCG) against porcine reproductive and respiratory syndrome virus infection in vitro","volume":"158","author":"Ge","year":"2018","journal-title":"Antivir Res"},{"issue":"19","key":"10.1016\/j.bmcl.2025.130162_bb0070","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1016\/j.vaccine.2020.03.019","article-title":"Oral supplementation of quercetin in PRRSV-1 modified-live virus vaccinated pigs in response to HP-PRRSV-2 challenge","volume":"38","author":"Ruansit","year":"2020","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0075","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s13567-019-0679-2","article-title":"Xanthohumol inhibits PRRSV proliferation and alleviates oxidative stress induced by PRRSV via the Nrf2\u2013HMOX1 axis","volume":"50","author":"Liu","year":"2019","journal-title":"Vet Res"},{"issue":"18","key":"10.1016\/j.bmcl.2025.130162_bb0080","doi-asserted-by":"crossref","first-page":"7107","DOI":"10.1080\/07391102.2020.1805364","article-title":"Exploring 3-hydroxyflavone scaffolds as mushroom tyrosinase inhibitors: synthesis, X-ray crystallography, antimicrobial, fluorescence behaviour, structure-activity relationship and molecular modelling studies","volume":"39","author":"Ashraf","year":"2021","journal-title":"J Biomol Struct Dyn"},{"issue":"9","key":"10.1016\/j.bmcl.2025.130162_bb0085","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1021\/acs.jcim.8b00285","article-title":"PADFrag: a database built for the exploration of bioactive fragment space for drug discovery","volume":"58","author":"Yang","year":"2018","journal-title":"J Chem Inf Model"},{"issue":"17","key":"10.1016\/j.bmcl.2025.130162_bb0090","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1016\/j.bmcl.2016.07.050","article-title":"A new class of flavonol-based anti-prostate cancer agents: design, synthesis, and evaluation in cell models","volume":"26","author":"Li","year":"2016","journal-title":"Bioorg Med Chem Lett"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0095","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s12985-023-02043-w","article-title":"Evaluating anti-viral effect of Tylvalosin tartrate on porcine reproductive and respiratory syndrome virus and analyzing the related gene regulation by transcriptomics","volume":"20","author":"Tang","year":"2023","journal-title":"Virol J"},{"issue":"2","key":"10.1016\/j.bmcl.2025.130162_bb0100","doi-asserted-by":"crossref","first-page":"e81","DOI":"10.1111\/j.1365-2893.2011.01507.x","article-title":"Suppression of hepatitis C virus by the flavonoid quercetin is mediated by inhibition of NS3 protease activity","volume":"19","author":"Bachmetov","year":"2012","journal-title":"J Viral Hepat"},{"issue":"6","key":"10.1016\/j.bmcl.2025.130162_bb0105","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1111\/cbdd.13604","article-title":"Characteristics of flavonoids as potent MERS-CoV 3C-like protease inhibitors","volume":"94","author":"Jo","year":"2019","journal-title":"Chem Biol Drug Des"},{"issue":"3","key":"10.1016\/j.bmcl.2025.130162_bb0110","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s10529-016-2261-6","article-title":"Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship","volume":"39","author":"Lim","year":"2017","journal-title":"Biotechnol Lett"},{"key":"10.1016\/j.bmcl.2025.130162_bb0115","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.virol.2022.04.005","article-title":"Flavonols and dihydroflavonols inhibit the main protease activity of SARS-CoV-2 and the replication of human coronavirus 229E","volume":"571","author":"Zhu","year":"2022","journal-title":"Virology"},{"issue":"1","key":"10.1016\/j.bmcl.2025.130162_bb0120","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1080\/14756366.2020.1754813","article-title":"Inhibition of African swine fever virus protease by myricetin and myricitrin","volume":"35","author":"Jo","year":"2020","journal-title":"J Enzyme Inhibition and Medicinal Chem"},{"key":"10.1016\/j.bmcl.2025.130162_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.virol.2024.110128","article-title":"Antiviral activity of flavonol against porcine epidemic diarrhea virus","volume":"597","author":"Liang","year":"2024","journal-title":"Virology"},{"issue":"9","key":"10.1016\/j.bmcl.2025.130162_bb0130","doi-asserted-by":"crossref","first-page":"4864","DOI":"10.1074\/jbc.271.9.4864","article-title":"The Arterivirus Nsp4 protease is the prototype of a novel Group of Chymotrypsin-like Enzymes, the 3C-like serine proteases","volume":"271","author":"Snijder","year":"1996","journal-title":"J Biol Chem"},{"issue":"2","key":"10.1016\/j.bmcl.2025.130162_bb0135","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J Comput Chem"},{"issue":"2","key":"10.1016\/j.bmcl.2025.130162_bb0140","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J Chem Theory Comput"},{"key":"10.1016\/j.bmcl.2025.130162_bb0145","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.vetmic.2017.11.031","article-title":"Identification of two antiviral inhibitors targeting 3C-like serine\/3C-like protease of porcine reproductive and respiratory syndrome virus and porcine epidemic diarrhea virus","volume":"213","author":"Shi","year":"2018","journal-title":"Vet Microbiol"},{"issue":"2","key":"10.1016\/j.bmcl.2025.130162_bb0150","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1002\/wcms.1121","article-title":"An overview of the Amber biomolecular simulation package","volume":"3","author":"Salomon-Ferrer","year":"2013","journal-title":"WIREs Computational Molecular Science"},{"issue":"16","key":"10.1016\/j.bmcl.2025.130162_bb0155","doi-asserted-by":"crossref","first-page":"9478","DOI":"10.1021\/acs.chemrev.9b00055","article-title":"End-point binding free energy calculation with MM\/PBSA and MM\/GBSA: strategies and applications in drug design","volume":"119","author":"Wang","year":"2019","journal-title":"Chem Rev"}],"container-title":["Bioorganic & Medicinal Chemistry Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960894X2500071X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960894X2500071X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:13:56Z","timestamp":1778789636000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960894X2500071X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":31,"alternative-id":["S0960894X2500071X"],"URL":"https:\/\/doi.org\/10.1016\/j.bmcl.2025.130162","relation":{},"ISSN":["0960-894X"],"issn-type":[{"value":"0960-894X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Discovery of Flavonol derivatives as porcine reproductive and respiratory syndrome virus inhibitors","name":"articletitle","label":"Article Title"},{"value":"Bioorganic & Medicinal Chemistry Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bmcl.2025.130162","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"130162"}}