{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T17:03:12Z","timestamp":1781974992034,"version":"3.54.5"},"reference-count":38,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bone"],"published-print":{"date-parts":[[2010,9]]},"DOI":"10.1016\/j.bone.2010.06.018","type":"journal-article","created":{"date-parts":[[2010,6,26]],"date-time":"2010-06-26T08:53:55Z","timestamp":1277542435000},"page":"657-665","source":"Crossref","is-referenced-by-count":25,"title":["The role of pyrophosphate\/phosphate homeostasis in terminal differentiation and apoptosis of growth plate chondrocytes"],"prefix":"10.1016","volume":"47","author":[{"given":"Hyon Jong","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Delaney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Kirsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bone.2010.06.018_bib1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1177\/154411130201300604","article-title":"The fate of the terminally differentiated chondrocyte: evidence for microenvironmental regulation of chondrocyte apoptosis","volume":"13","author":"Adams","year":"2002","journal-title":"Crit Rev Oral Biol Med"},{"key":"10.1016\/j.bone.2010.06.018_bib2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF00508369","article-title":"Energy dispersive X-ray elemental analysis of isolated epiphyseal growth plate chondrocyte fragments","volume":"69","author":"Boyde","year":"1980","journal-title":"Histochemistry"},{"key":"10.1016\/j.bone.2010.06.018_bib3","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0221-8747(84)90019-5","article-title":"Microdissection\u2013elemental analysis of the mineralizing growth cartilage of the normal and rachitic chick","volume":"5","author":"Shapiro","year":"1984","journal-title":"Metab Bone Dis Relat Res"},{"key":"10.1016\/j.bone.2010.06.018_bib4","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1002\/jcb.240570206","article-title":"Morphological and biochemical characterization of mineralizing primary cultures of avian growth plate chondrocytes: evidence for cellular processing of Ca2+ and Pi prior to matrix mineralization","volume":"57","author":"Wu","year":"1995","journal-title":"J Cell Biochem"},{"key":"10.1016\/j.bone.2010.06.018_bib5","doi-asserted-by":"crossref","first-page":"9637","DOI":"10.1073\/pnas.0502249102","article-title":"Hypophosphatemia leads to rickets by impairing caspase-mediated apoptosis of hypertrophic chondrocytes","volume":"102","author":"Sabbagh","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.bone.2010.06.018_bib6","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s00223-002-1056-z","article-title":"Induction of apoptosis in skeletal tissues: phosphate-mediated chick chondrocyte apoptosis is calcium dependent","volume":"73","author":"Mansfield","year":"2003","journal-title":"Calcif Tissue Int"},{"key":"10.1016\/j.bone.2010.06.018_bib7","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1002\/(SICI)1097-4652(199906)179:3<276::AID-JCP5>3.0.CO;2-#","article-title":"Extracellular phosphate ions cause apoptosis of terminally differentiated epiphyseal chondrocytes","volume":"179","author":"Mansfield","year":"1999","journal-title":"J Cell Physiol"},{"key":"10.1016\/j.bone.2010.06.018_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S8756-3282(00)00409-9","article-title":"Phosphate ions mediate chondrocyte apoptosis through a plasma membrane transporter mechanism","volume":"28","author":"Mansfield","year":"2001","journal-title":"Bone"},{"key":"10.1016\/j.bone.2010.06.018_bib9","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1359\/jbmr.2003.18.8.1430","article-title":"Phosphate is a specific signal for ATDC5 chondrocyte maturation and apoptosis-associated mineralization: possible implication of apoptosis in the regulation of endochondral ossification","volume":"18","author":"Magne","year":"2003","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.bone.2010.06.018_bib10","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1359\/jbmr.1999.14.12.2015","article-title":"Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia","volume":"14","author":"Fedde","year":"1999","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.bone.2010.06.018_bib11","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1126\/science.289.5477.265","article-title":"Role of the mouse ank gene in control of tissue calcification and arthritis","volume":"289","author":"Ho","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.bone.2010.06.018_bib12","doi-asserted-by":"crossref","first-page":"C1","DOI":"10.1152\/ajpcell.2001.281.1.C1","article-title":"Inorganic pyrophosphate generation and disposition in pathophysiology","volume":"281","author":"Terkeltaub","year":"2001","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.bone.2010.06.018_bib13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0065-2571(99)00036-9","article-title":"Molecular mechanisms of mammalian inorganic phosphate homeostasis","volume":"40","author":"Takeda","year":"2000","journal-title":"Adv Enzyme Regul"},{"key":"10.1016\/j.bone.2010.06.018_bib14","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/ki.1996.138","article-title":"Characteristics and regulation of Pi transport in osteogenic cells for bone metabolism","volume":"49","author":"Caverzasio","year":"1996","journal-title":"Kidney Int"},{"key":"10.1016\/j.bone.2010.06.018_bib15","doi-asserted-by":"crossref","first-page":"15872","DOI":"10.1074\/jbc.M701116200","article-title":"Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity","volume":"282","author":"Addison","year":"2007","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bone.2010.06.018_bib16","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1083\/jcb.137.5.1149","article-title":"Regulated production of mineralization-competent matrix vesicles in hypertrophic chondrocytes","volume":"137","author":"Kirsch","year":"1997","journal-title":"J Cell Biol"},{"key":"10.1016\/j.bone.2010.06.018_bib17","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/0945-053X(94)90199-6","article-title":"Characterization of primary cultures of chondrocytes from type II collagen\/beta-galactosidase transgenic mice","volume":"14","author":"Lefebvre","year":"1994","journal-title":"Matrix Biol"},{"key":"10.1016\/j.bone.2010.06.018_bib18","doi-asserted-by":"crossref","first-page":"30848","DOI":"10.1074\/jbc.M605937200","article-title":"Annexin V\/beta5 integrin interactions regulate apoptosis of growth plate chondrocytes","volume":"281","author":"Wang","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bone.2010.06.018_bib19","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1128\/MCB.25.1.312-323.2005","article-title":"Role of the progressive ankylosis gene (ank) in cartilage mineralization","volume":"25","author":"Wang","year":"2005","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.bone.2010.06.018_bib20","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1083\/jcb.200203014","article-title":"Retinoic acid stimulates annexin-mediated growth plate chondrocyte mineralization","volume":"157","author":"Wang","year":"2002","journal-title":"J Cell Biol"},{"key":"10.1016\/j.bone.2010.06.018_bib21","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1002\/jcp.20857","article-title":"Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation","volume":"210","author":"Pucci","year":"2007","journal-title":"J Cell Physiol"},{"key":"10.1016\/j.bone.2010.06.018_bib22","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1006\/excr.1993.1209","article-title":"Retinoic acid induces rapid mineralization and expression of mineralization-related genes in chondrocytes","volume":"207","author":"Iwamoto","year":"1993","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.bone.2010.06.018_bib23","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1093\/clinchem\/22.7.972","article-title":"Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans","volume":"22","author":"Van Belle","year":"1976","journal-title":"Clin Chem"},{"key":"10.1016\/j.bone.2010.06.018_bib24","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1093\/rheumatology\/40.10.1146","article-title":"Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor","volume":"40","author":"Kolettas","year":"2001","journal-title":"Rheumatology"},{"key":"10.1016\/j.bone.2010.06.018_bib25","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1002\/art.20748","article-title":"Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes","volume":"52","author":"Cecil","year":"2005","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.bone.2010.06.018_bib26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1006\/dbio.1999.9207","article-title":"Retinoid signaling is required for chondrocyte maturation and endochondral bone formation during limb skeletogenesis","volume":"208","author":"Koyama","year":"1999","journal-title":"Dev Biol"},{"key":"10.1016\/j.bone.2010.06.018_bib27","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/jn\/123.suppl_2.301","article-title":"Involvement of cellular metabolism of calcium and phosphate in calcification of avian growth plate cartilage","volume":"123","author":"Wuthier","year":"1993","journal-title":"J Nutr"},{"key":"10.1016\/j.bone.2010.06.018_bib28","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S0014-4827(03)00213-1","article-title":"Inorganic phosphate regulates multiple genes during osteoblast differentiation, including Nrf2","volume":"288","author":"Beck","year":"2003","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.bone.2010.06.018_bib29","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/9467","article-title":"VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation","volume":"5","author":"Gerber","year":"1999","journal-title":"Nat Med"},{"key":"10.1016\/j.bone.2010.06.018_bib30","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/(SICI)1097-0177(199703)208:3<387::AID-AJA9>3.0.CO;2-E","article-title":"Collagenase-3 (MMP-13) is expressed by hypertrophic chondrocytes, periosteal cells, and osteoblasts during human fetal bone development","volume":"208","author":"Johansson","year":"1997","journal-title":"Dev Dyn"},{"key":"10.1016\/j.bone.2010.06.018_bib31","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1359\/jbmr.2000.15.7.1257","article-title":"Collagenase-3 (MMP-13) and integral membrane protein 2a (Itm2a) are marker genes of chondrogenic\/osteoblastic cells in bone formation: sequential temporal, and spatial expression of Itm2a, alkaline phosphatase, MMP-13, and osteocalcin in the mouse","volume":"15","author":"Tuckermann","year":"2000","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.bone.2010.06.018_bib32","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1086\/432261","article-title":"Dysregulation of chondrogenesis in human cleidocranial dysplasia","volume":"77","author":"Zheng","year":"2005","journal-title":"Am J Hum Genet"},{"key":"10.1016\/j.bone.2010.06.018_bib33","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.joca.2004.08.008","article-title":"Regulation of MMP-13 expression by RUNX2 and FGF2 in osteoarthritic cartilage","volume":"12","author":"Wang","year":"2004","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.bone.2010.06.018_bib34","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1002\/art.22041","article-title":"Contribution of runt-related transcription factor 2 to the pathogenesis of osteoarthritis in mice after induction of knee joint instability","volume":"54","author":"Kamekura","year":"2006","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.bone.2010.06.018_bib35","doi-asserted-by":"crossref","first-page":"47","DOI":"10.2174\/138920108783497659","article-title":"Joint diseases and matrix metalloproteinases: a role for MMP-13","volume":"9","author":"Takaishi","year":"2008","journal-title":"Curr Pharm Biotechnol"},{"key":"10.1016\/j.bone.2010.06.018_bib36","doi-asserted-by":"crossref","first-page":"3218","DOI":"10.1002\/art.10632","article-title":"Up-regulated expression of cartilage intermediate-layer protein and ANK in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease","volume":"46","author":"Hirose","year":"2002","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.bone.2010.06.018_bib37","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.joca.2003.12.004","article-title":"Upregulated ank expression in osteoarthritis can promote both chondrocyte MMP-13 expression and calcification via chondrocyte extracellular PPi excess","volume":"12","author":"Johnson","year":"2004","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.bone.2010.06.018_bib38","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.joca.2008.05.020","article-title":"Inorganic pyrophosphate as a regulator of hydroxyapatite or calcium pyrophosphate dihydrate mineral deposition by matrix vesicles","volume":"17","author":"Thouverey","year":"2009","journal-title":"Osteoarthritis Cartilage"}],"container-title":["Bone"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328210013116?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328210013116?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T05:13:01Z","timestamp":1591420381000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S8756328210013116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["S8756328210013116"],"URL":"https:\/\/doi.org\/10.1016\/j.bone.2010.06.018","relation":{},"ISSN":["8756-3282"],"issn-type":[{"value":"8756-3282","type":"print"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}