{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T06:10:00Z","timestamp":1774419000476,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004298","name":"Secom Science and Technology Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004298","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K12866ZA"],"award-info":[{"award-number":["JP24K12866ZA"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K24467"],"award-info":[{"award-number":["JP23K24467"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bone"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.bone.2025.117473","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T22:37:44Z","timestamp":1743028664000},"page":"117473","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Visualization and quantification of RANK-RANKL binding for application to disease investigations and drug discovery"],"prefix":"10.1016","volume":"195","author":[{"given":"Ken-ichi","family":"Nakahama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiho","family":"Hidaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanako","family":"Goto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayu","family":"Tada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoya","family":"Doi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Akiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"7803","key":"10.1016\/j.bone.2025.117473_bb0005","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/s41586-020-2188-x","article-title":"A reference map of the human binary protein interactome","volume":"580","author":"Luck","year":"2020","journal-title":"Nature"},{"issue":"23","key":"10.1016\/j.bone.2025.117473_bb0010","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1016\/j.cell.2024.10.038","article-title":"Discovery and significance of protein-protein interactions in health and disease","volume":"187","author":"Greenblatt","year":"2024","journal-title":"Cell"},{"issue":"8","key":"10.1016\/j.bone.2025.117473_bb0015","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.tibtech.2010.05.002","article-title":"Lighting up multiprotein complexes: lessons from GPCR oligomerization","volume":"28","author":"Ciruela","year":"2010","journal-title":"Trends Biotechnol."},{"issue":"2","key":"10.1016\/j.bone.2025.117473_bb0020","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1021\/acschembio.5b00753","article-title":"NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells","volume":"11","author":"Dixon","year":"2016","journal-title":"ACS Chem. Biol."},{"issue":"4","key":"10.1016\/j.bone.2025.117473_bb0025","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1093\/ejendo\/lvad039","article-title":"Characterization of dominant-negative growth hormone receptor variants reveals a potential therapeutic target for short stature","volume":"188","author":"Andrews","year":"2023","journal-title":"Eur. J. Endocrinol."},{"issue":"5","key":"10.1016\/j.bone.2025.117473_bb0030","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1021\/acsmeasuresciau.3c00021","article-title":"Accessible and generalizable in vitro luminescence assay for detecting GPCR activation","volume":"3","author":"Miller","year":"2023","journal-title":"ACS Measur. Sci. au"},{"issue":"1","key":"10.1016\/j.bone.2025.117473_bb0035","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1186\/s12964-023-01146-9","article-title":"Analysis of RAS and drug induced homo- and heterodimerization of RAF and KSR1 proteins in living cells using split Nanoluc luciferase","volume":"21","author":"Rohrer","year":"2023","journal-title":"Cell Commun. Signal."},{"issue":"11","key":"10.1016\/j.bone.2025.117473_bb0040","doi-asserted-by":"crossref","first-page":"4055","DOI":"10.1021\/acssensors.3c01080","article-title":"Split luciferase-fragment reconstitution for unveiling RNA localization and dynamics in live cells","volume":"8","author":"Eguchi","year":"2023","journal-title":"ACS Sens."},{"issue":"23","key":"10.1016\/j.bone.2025.117473_bb0045","doi-asserted-by":"crossref","first-page":"4001","DOI":"10.1007\/s00018-010-0479-3","article-title":"Cellular communications in bone homeostasis and repair","volume":"67","author":"Nakahama","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bone.2025.117473_bb0050","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bone.2013.12.031","article-title":"Communication-dependent mineralization of osteoblasts via gap junctions","volume":"61","author":"Hashida","year":"2014","journal-title":"Bone"},{"issue":"Pt 20","key":"10.1016\/j.bone.2025.117473_bb0055","doi-asserted-by":"crossref","first-page":"4187","DOI":"10.1242\/jcs.03162","article-title":"Low peak bone mass and attenuated anabolic response to parathyroid hormone in mice with an osteoblast-specific deletion of connexin43","volume":"119","author":"Chung","year":"2006","journal-title":"J. Cell Sci."},{"issue":"8","key":"10.1016\/j.bone.2025.117473_bb0060","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1091\/mbc.e10-07-0571","article-title":"Osteoblast connexin43 modulates skeletal architecture by regulating both arms of bone remodeling","volume":"22","author":"Watkins","year":"2011","journal-title":"Mol. Biol. Cell"},{"issue":"8","key":"10.1016\/j.bone.2025.117473_bb0065","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/ng2076","article-title":"Osteoclast-poor human osteopetrosis due to mutations in the gene encoding RANKL","volume":"39","author":"Sobacchi","year":"2007","journal-title":"Nat. Genet."},{"issue":"4","key":"10.1016\/j.bone.2025.117473_bb0070","doi-asserted-by":"crossref","first-page":"2800","DOI":"10.1002\/jcp.30045","article-title":"A missense mutation sheds light on a novel structure-function relationship of RANKL","volume":"236","author":"Qiu","year":"2021","journal-title":"J. Cell. Physiol."},{"issue":"4","key":"10.1016\/j.bone.2025.117473_bb0075","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1093\/hmg\/ddr510","article-title":"A RANKL G278R mutation causing osteopetrosis identifies a functional amino acid essential for trimer assembly in RANKL and TNF","volume":"21","author":"Douni","year":"2012","journal-title":"Hum. Mol. Genet."},{"issue":"1","key":"10.1016\/j.bone.2025.117473_bb0080","doi-asserted-by":"crossref","first-page":"5338","DOI":"10.1038\/s41467-022-33006-4","article-title":"Identification of a binding site on soluble RANKL that can be targeted to inhibit soluble RANK-RANKL interactions and treat osteoporosis","volume":"13","author":"Huang","year":"2022","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.bone.2025.117473_bb0085","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ajhg.2008.06.015","article-title":"Human osteoclast-poor osteopetrosis with hypogammaglobulinemia due to TNFRSF11A (RANK) mutations","volume":"83","author":"Guerrini","year":"2008","journal-title":"Am. J. Hum. Genet."},{"issue":"20","key":"10.1016\/j.bone.2025.117473_bb0090","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1002\/cmdc.201700462","article-title":"Porphyrin derivatives inhibit the interaction between receptor activator of NF-\u03baB and its ligand","volume":"12","author":"Chypre","year":"2017","journal-title":"ChemMedChem"},{"key":"10.1016\/j.bone.2025.117473_bb0095","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2023.115502","article-title":"Isoimperatorin attenuates bone loss by inhibiting the binding of RANKL to RANK","volume":"211","author":"Li","year":"2023","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bone.2025.117473_bb0100","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2023.115463","article-title":"Isoliensinine suppresses bone loss by targeted inhibition of RANKL-RANK binding","volume":"210","author":"Deng","year":"2023","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bone.2025.117473_bb0105","article-title":"Discovery of sp3-rich diazatricycloundecanes as lysosomotropic autophagy inhibitors Eur","volume":"280","author":"Miura","year":"2024","journal-title":"J. Med. Chem."},{"issue":"42","key":"10.1016\/j.bone.2025.117473_bb0110","doi-asserted-by":"crossref","first-page":"37023","DOI":"10.1074\/jbc.M111.246280","article-title":"Increased bone mass in mice after single injection of anti-receptor activator of nuclear factor-kappaB ligand-neutralizing antibody: evidence for bone anabolic effect of parathyroid hormone in mice with few osteoclasts","volume":"286","author":"Furuya","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bone.2025.117473_bb0115","doi-asserted-by":"crossref","first-page":"463","DOI":"10.2147\/PPA.S46192","article-title":"A review of denosumab for the treatment of osteoporosis","volume":"8","author":"Miyazaki","year":"2014","journal-title":"Patient Prefer. Adher."}],"container-title":["Bone"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328225000857?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328225000857?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T05:18:44Z","timestamp":1774415924000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S8756328225000857"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":23,"alternative-id":["S8756328225000857"],"URL":"https:\/\/doi.org\/10.1016\/j.bone.2025.117473","relation":{},"ISSN":["8756-3282"],"issn-type":[{"value":"8756-3282","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Visualization and quantification of RANK-RANKL binding for application to disease investigations and drug discovery","name":"articletitle","label":"Article Title"},{"value":"Bone","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bone.2025.117473","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117473"}}