{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:07:25Z","timestamp":1781222845487,"version":"3.54.1"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"National Institute of Allergy and Infectious Diseases Division of Intramural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bone"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.bone.2025.117689","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T08:12:19Z","timestamp":1761379939000},"page":"117689","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["The C-type lectin receptor mincle is functionally expressed by murine bone cells and can mediate inflammatory osteoblast responses to Staphylococcus aureus"],"prefix":"10.1016","volume":"203","author":[{"given":"Erin L.","family":"Mills","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quinton A.","family":"Krueger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiza","family":"Noyal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Brittany","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Marriott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.bone.2025.117689_bb0005","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1385\/IR:30:3:291","article-title":"Osteoblast responses to bacterial pathogens: a previously unappreciated role for bone-forming cells in host defense and disease progression","volume":"30","author":"Marriott","year":"2004","journal-title":"Immunol. Res."},{"issue":"8","key":"10.1016\/j.bone.2025.117689_bb0010","doi-asserted-by":"crossref","first-page":"4075","DOI":"10.1128\/IAI.70.8.4075-4082.2002","article-title":"Bacterium-induced CXCL10 secretion by osteoblasts can be mediated in part through toll-like receptor 4","volume":"70","author":"Gasper","year":"2002","journal-title":"Infect. Immun."},{"issue":"9","key":"10.1016\/j.bone.2025.117689_bb0015","doi-asserted-by":"crossref","first-page":"5418","DOI":"10.1128\/IAI.71.9.5418-5421.2003","article-title":"Signaling via toll-like receptor 5 can initiate inflammatory mediator production by murine osteoblasts","volume":"71","author":"Madrazo","year":"2003","journal-title":"Infect. Immun."},{"issue":"19","key":"10.1016\/j.bone.2025.117689_bb0020","doi-asserted-by":"crossref","first-page":"16732","DOI":"10.1074\/jbc.M212473200","article-title":"Cpg oligodeoxynucleotides modulate the osteoclastogenic activity of osteoblasts via toll-like receptor 9","volume":"278","author":"Zou","year":"2003","journal-title":"J. Biol. Chem."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0025","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1128\/IAI.73.5.2967-2973.2005","article-title":"Induction of Nod1 and Nod2 intracellular pattern recognition receptors in murine osteoblasts following bacterial challenge","volume":"73","author":"Marriott","year":"2005","journal-title":"Infect. Immun."},{"issue":"3","key":"10.1016\/j.bone.2025.117689_bb0030","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.4049\/jimmunol.180.3.1382","article-title":"Stimulation by TLR5 modulates osteoclast differentiation through STAT1\/IFN-beta","volume":"180","author":"Ha","year":"2008","journal-title":"J. Immunol."},{"issue":"3","key":"10.1016\/j.bone.2025.117689_bb0035","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10616-012-9442-5","article-title":"R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts","volume":"64","author":"Miyamoto","year":"2012","journal-title":"Cytotechnology"},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0040","doi-asserted-by":"crossref","DOI":"10.1186\/s12866-016-0855-8","article-title":"TLR9 mediates S. aureus killing inside osteoblasts via induction of oxidative stress","volume":"16","author":"Mohamed","year":"2016","journal-title":"BMC Microbiol."},{"issue":"12","key":"10.1016\/j.bone.2025.117689_bb0045","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1016\/j.jcms.2017.09.007","article-title":"TLR expression profile of human alveolar bone proper-derived stem\/progenitor cells and osteoblasts","volume":"45","author":"Fawzy El-Sayed","year":"2017","journal-title":"J. Craniomaxillofac. Surg."},{"issue":"4","key":"10.1016\/j.bone.2025.117689_bb0050","doi-asserted-by":"crossref","first-page":"227","DOI":"10.11005\/jbm.2020.27.4.227","article-title":"The role of toll-like receptors in osteoclastogenesis","volume":"27","author":"Yim","year":"2020","journal-title":"J Bone Metab."},{"issue":"6","key":"10.1016\/j.bone.2025.117689_bb0055","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/intimm\/dxy028","article-title":"Mincle: 20 years of a versatile sensor of insults","volume":"30","author":"Lu","year":"2018","journal-title":"Int. Immunol."},{"issue":"12","key":"10.1016\/j.bone.2025.117689_bb0060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006038","article-title":"C-type lectin Mincle recognizes Glucosyl-diacylglycerol of Streptococcus pneumoniae and plays a protective role in pneumococcal pneumonia","volume":"12","author":"Behler-Janbeck","year":"2016","journal-title":"PLoS Pathog."},{"issue":"2","key":"10.1016\/j.bone.2025.117689_bb0065","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/0014-5793(75)80013-5","article-title":"Lipoteichoic acid and lipoteichoic acid carrier in Staphylococcus aureus H","volume":"53","author":"Duckworth","year":"1975","journal-title":"FEBS Lett."},{"issue":"11","key":"10.1016\/j.bone.2025.117689_bb0070","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.1128\/JB.183.11.3506-3514.2001","article-title":"Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase","volume":"183","author":"Kiriukhin","year":"2001","journal-title":"J. Bacteriol."},{"issue":"6","key":"10.1016\/j.bone.2025.117689_bb0075","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1128\/JB.01683-06","article-title":"Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus","volume":"189","author":"Gr\u00fcndling","year":"2007","journal-title":"J. Bacteriol."},{"issue":"6","key":"10.1016\/j.bone.2025.117689_bb0080","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s00109-010-0630-5","article-title":"Penetration of the blood-brain barrier by Staphylococcus aureus: contribution of membrane-anchored lipoteichoic acid","volume":"88","author":"Sheen","year":"2010","journal-title":"J. Mol. Med. (Berl)"},{"issue":"12","key":"10.1016\/j.bone.2025.117689_bb0085","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1046\/j.1432-1327.2000.01414.x","article-title":"Novel processive and nonprocessive glycosyltransferases from Staphylococcus aureus and Arabidopsis thaliana synthesize glycoglycerolipids, glycophospholipids, glycosphingolipids and glycosylsterols","volume":"267","author":"Jorasch","year":"2000","journal-title":"Eur. J. Biochem."},{"issue":"11","key":"10.1016\/j.bone.2025.117689_bb0090","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.1128\/JB.183.11.3506-3514.2001","article-title":"Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase","volume":"183","author":"Kiriukhin","year":"2001","journal-title":"J. Bacteriol."},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0095","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1093\/infdis\/jiad608","article-title":"Ectopic expression of C-type lectin Mincle renders mice susceptible to staphylococcal pneumonia","volume":"230","author":"Hollwedel","year":"2024","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.bone.2025.117689_bb0100","doi-asserted-by":"crossref","first-page":"3119","DOI":"10.2147\/JIR.S363538","article-title":"Comparative transcriptome analysis of gingival immune-mediated inflammation in Peri-Implantitis and periodontitis within the same host environment","volume":"15","author":"Yuan","year":"2022","journal-title":"J. Inflamm. Res."},{"issue":"9","key":"10.1016\/j.bone.2025.117689_bb0105","doi-asserted-by":"crossref","first-page":"4811","DOI":"10.1172\/JCI134214","article-title":"Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin","volume":"130","author":"Andreev","year":"2020","journal-title":"J. Clin. Invest."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0110","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1089\/107999001300177484","article-title":"Monocyte chemoattractant protein-1 expression by osteoblasts following infection with Staphylococcus aureus or Salmonella","volume":"21","author":"Bost","year":"2001","journal-title":"J. Interf. Cytokine Res."},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0115","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1007\/s10753-022-01731-z","article-title":"Substance P exacerbates the inflammatory and pro-osteoclastogenic responses of murine osteoclasts and osteoblasts to Staphylococcus aureus","volume":"46","author":"Johnson","year":"2023","journal-title":"Inflammation"},{"issue":"3","key":"10.1016\/j.bone.2025.117689_bb0120","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1128\/IAI.71.3.1209-1216.2003","article-title":"Functional CD40 expression induced following bacterial infection of mouse and human osteoblasts","volume":"71","author":"Schrum","year":"2003","journal-title":"Infect. Immun."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0125","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/S8756-3282(03)00265-5","article-title":"Bacterial infection induces expression of functional MHC class II molecules in murine and human osteoblasts","volume":"33","author":"Schrum","year":"2003","journal-title":"Bone"},{"key":"10.1016\/j.bone.2025.117689_bb0130","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1007\/BF03354640","article-title":"The isolation and culture of cells from explants of human trabecular bone","volume":"56","author":"Grundle","year":"1995","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.bone.2025.117689_bb0135","article-title":"Immunostimulatory nucleic acid nanoparticles (NANPs) augment protective osteoblast and osteoclast type I interferon responses to Staphylococcus aureus","volume":"60","author":"Mills","year":"2024","journal-title":"Nanomedicine"},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0140","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1189\/jlb.3A0515-185R","article-title":"Mincle suppresses Toll-like receptor 4 activation","volume":"100","author":"Greco","year":"2016","journal-title":"J. Leukoc. Biol."},{"issue":"17","key":"10.1016\/j.bone.2025.117689_bb0145","first-page":"3599","article-title":"Profiling gene expression responses of coral larvae (Acropora millepora) to elevated temperature and settlement inducers using a novel RNA-Seq procedure","volume":"20","author":"Meyer","year":"2011","journal-title":"Mol. Ecol."},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0150","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1006\/geno.1997.4995","article-title":"Comparison of DNA sequences with protein sequences","volume":"46","author":"Pearson","year":"1997","journal-title":"Genomics"},{"issue":"12","key":"10.1016\/j.bone.2025.117689_bb0155","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"issue":"3","key":"10.1016\/j.bone.2025.117689_bb0160","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1093\/bioinformatics\/btn647","article-title":"arrayQualityMetrics--a bioconductor package for quality assessment of microarray data","volume":"25","author":"Kauffmann","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.bone.2025.117689_bb0165","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2009"},{"issue":"8","key":"10.1016\/j.bone.2025.117689_bb0170","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0175","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00814-20","article-title":"mRNA transcriptome analysis of bone in a mouse model of implant-associated Staphylococcus aureus osteomyelitis","volume":"89","author":"Lin","year":"2021","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bone.2025.117689_bb0180","doi-asserted-by":"crossref","DOI":"10.1007\/s10753-025-02280-x","article-title":"Substance P augments chemokine production by Staphylococcus aureus infected murine osteoclasts","author":"Sipprell","year":"2025","journal-title":"Inflammation"},{"issue":"4","key":"10.1016\/j.bone.2025.117689_bb0185","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00014-23","article-title":"Staphylococcus aureus infection induces the production of the neutrophil chemoattractants CXCL1, CXCL2, CXCL3, CXCL5, CCL3, and CCL7 by murine osteoblasts","volume":"91","author":"Sipprell","year":"2023","journal-title":"Infect. Immun."},{"issue":"Pt 7","key":"10.1016\/j.bone.2025.117689_bb0190","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1099\/jmm.0.015859-0","article-title":"Differential roles for NOD2 in osteoblast inflammatory immune responses to bacterial pathogens of bone tissue","volume":"59","author":"Chauhan","year":"2010","journal-title":"J. Med. Microbiol."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0195","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.03971-24","article-title":"RIG-I and cGAS mediate antimicrobial and inflammatory responses of primary osteoblasts and osteoclasts to Staphylococcus aureus","volume":"16","author":"Mills","year":"2025","journal-title":"MBio"},{"issue":"1","key":"10.1016\/j.bone.2025.117689_bb0200","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.bone.2025.117689_bb0205","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.4049\/jimmunol.0904013","article-title":"Cutting edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate","volume":"184","author":"Schoenen","year":"2010","journal-title":"J. Immunol."},{"issue":"16","key":"10.1016\/j.bone.2025.117689_bb0210","doi-asserted-by":"crossref","first-page":"E3285","DOI":"10.1073\/pnas.1618133114","article-title":"Intracellular metabolite \u03b2-glucosylceramide is an endogenous Mincle ligand possessing immunostimulatory activity","volume":"114","author":"Nagata","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bone.2025.117689_bb0215","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.00251","article-title":"C-type lectin-like receptors: head or tail in cell death immunity","volume":"11","author":"Drouin","year":"2020","journal-title":"Front. Immunol."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0220","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.1128\/IAI.67.5.2677-2681.1999","article-title":"Mechanisms of internalization of Staphylococcus aureus by cultured human osteoblasts","volume":"67","author":"Jevon","year":"1999","journal-title":"Infect. Immun."},{"issue":"5","key":"10.1016\/j.bone.2025.117689_bb0225","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1128\/IAI.69.5.2872-2877.2001","article-title":"Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria","volume":"69","author":"Ahmed","year":"2001","journal-title":"Infect. Immun."},{"issue":"4","key":"10.1016\/j.bone.2025.117689_bb0230","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1021\/acsnano.8b00716","article-title":"Mechanical forces guiding Staphylococcus aureus cellular invasion","volume":"12","author":"Prystopiuk","year":"2018","journal-title":"ACS Nano"}],"updated-by":[{"DOI":"10.1016\/j.bone.2026.117936","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000}},{"DOI":"10.1016\/j.bone.2026.117936","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000}}],"container-title":["Bone"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328225003011?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328225003011?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T23:44:49Z","timestamp":1781221489000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S8756328225003011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":46,"alternative-id":["S8756328225003011"],"URL":"https:\/\/doi.org\/10.1016\/j.bone.2025.117689","relation":{},"ISSN":["8756-3282"],"issn-type":[{"value":"8756-3282","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The C-type lectin receptor mincle is functionally expressed by murine bone cells and can mediate inflammatory osteoblast responses to Staphylococcus aureus","name":"articletitle","label":"Article Title"},{"value":"Bone","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bone.2025.117689","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117689"}}