{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T22:00:25Z","timestamp":1781215225232,"version":"3.54.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007710","name":"University of Connecticut","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE027642"],"award-info":[{"award-number":["R01DE027642"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bone"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.bone.2026.117852","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:01:36Z","timestamp":1773273696000},"page":"117852","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Porphyromonas gingivalis lipids enhance RANKL-mediated osteoclast formation"],"prefix":"10.1016","volume":"207","author":[{"given":"Matthew A.","family":"Zambrello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Sanjay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Rahmlow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Etoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Lamm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Sobue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavya","family":"Uddaraju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank C.","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.bone.2026.117852_bb0005","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.cden.2005.03.003","article-title":"Epidemiology and risk factors of periodontal diseases","volume":"49","author":"Albandar","year":"2005","journal-title":"Dent. Clin. N. Am."},{"issue":"2","key":"10.1016\/j.bone.2026.117852_bb0010","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1600-051X.1998.tb02419.x","article-title":"Microbial complexes in subgingival plaque","volume":"25","author":"Socransky","year":"1998","journal-title":"J. Clin. Periodontol."},{"key":"10.1016\/j.bone.2026.117852_bb0015","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1902\/jop.1988.59.4.259","article-title":"Intraoral occurance of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in active destructive periodontal lesions","volume":"59","author":"Saglie","year":"1988","journal-title":"J. Periodontol."},{"issue":"3","key":"10.1016\/j.bone.2026.117852_bb0020","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1034\/j.1600-0765.2001.360302.x","article-title":"Prostaglandin E2 secretion from gingival fibroblasts treated with interleukin-1beta: effects of lipid extracts from Porphyromonas gingivalis or calculus","volume":"36","author":"Nichols","year":"2001","journal-title":"J. Periodontal Res."},{"issue":"2","key":"10.1016\/j.bone.2026.117852_bb0025","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1399-302X.2006.00264.x","article-title":"Porphyromonas gingivalis lipids and diseased dental tissues","volume":"21","author":"Nichols","year":"2006","journal-title":"Oral Microbiol. Immunol."},{"issue":"9","key":"10.1016\/j.bone.2026.117852_bb0030","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1128\/IAI.00803-13","article-title":"Serine lipids of Porphyromonas gingivalis are human and mouse Toll-like receptor 2 ligands","volume":"81","author":"Clark","year":"2013","journal-title":"Infect. Immun."},{"issue":"4","key":"10.1016\/j.bone.2026.117852_bb0035","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00877-19","article-title":"Glycine lipids of Porphyromonas gingivalis are agonists for toll-like receptor 2","volume":"88","author":"Nichols","year":"2020","journal-title":"Infect. Immun."},{"issue":"12","key":"10.1016\/j.bone.2026.117852_bb0040","doi-asserted-by":"crossref","first-page":"8296","DOI":"10.4049\/jimmunol.177.12.8296","article-title":"Cutting edge: TLR2 is required for the innate response to Porphyromonas gingivalis: activation leads to bacterial persistence and TLR2 deficiency attenuates induced alveolar bone resorption","volume":"177","author":"Burns","year":"2006","journal-title":"J. Immunol."},{"issue":"9","key":"10.1016\/j.bone.2026.117852_bb0045","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1128\/IAI.00225-10","article-title":"Porphyromonas gingivalis lipids inhibit osteoblastic differentiation and function","volume":"78","author":"Wang","year":"2010","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bone.2026.117852_bb0050","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.bone.2015.09.008","article-title":"Serine dipeptide lipids of Porphyromonas gingivalis inhibit osteoblast differentiation: relationship to Toll-like receptor 2","volume":"81","author":"Wang","year":"2015","journal-title":"Bone"},{"issue":"5","key":"10.1016\/j.bone.2026.117852_bb0055","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1016\/S0002-9440(10)63560-2","article-title":"Receptor activator of nuclear factor kappaB ligand (RANKL)\/osteoprotegerin (OPG) ratio is increased in severe osteolysis","volume":"163","author":"Grimaud","year":"2003","journal-title":"Am. J. Pathol."},{"issue":"11","key":"10.1016\/j.bone.2026.117852_bb0060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0277871","article-title":"Evaluation of culture conditions for osteoclastogenesis in RAW264.7 cells","volume":"17","author":"Cheng","year":"2022","journal-title":"PLoS One"},{"issue":"5","key":"10.1016\/j.bone.2026.117852_bb0065","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.bbalip.2017.01.008","article-title":"Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor","volume":"1862","author":"Kanzaki","year":"2017","journal-title":"Biochim. Biophys. Acta"},{"issue":"27","key":"10.1016\/j.bone.2026.117852_bb0070","doi-asserted-by":"crossref","first-page":"24159","DOI":"10.1074\/jbc.M110.198085","article-title":"TLR2-dependent modulation of osteoclastogenesis by Porphyromonas gingivalis through differential induction of NFATc1 and NF-kappaB","volume":"286","author":"Zhang","year":"2011","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.bone.2026.117852_bb0075","doi-asserted-by":"crossref","first-page":"987","DOI":"10.2353\/ajpath.2006.060180","article-title":"B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease","volume":"169","author":"Kawai","year":"2006","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.bone.2026.117852_bb0080","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.3389\/fimmu.2020.01693","article-title":"Chronic exposure of gingival fibroblasts to TLR2 or TLR4 agonist inhibits osteoclastogenesis but does not affect osteogenesis","volume":"11","author":"Karlis","year":"2020","journal-title":"Front. Immunol."},{"issue":"03","key":"10.1016\/j.bone.2026.117852_bb0085","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/omi.12439","article-title":"Metabolism of serine\/glycine lipids by human gingival cells in culture","volume":"39","author":"Guido","year":"2023","journal-title":"Mol Oral Microbiol"},{"issue":"9","key":"10.1016\/j.bone.2026.117852_bb0090","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1128\/iai.62.9.3753-3760.1994","article-title":"Distribution of 3-hydroxy iC17:0 in subgingival plaque and gingival tissue samples: relationship to adult periodontitis","volume":"62","author":"Nichols","year":"1994","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bone.2026.117852_bb0095","doi-asserted-by":"crossref","first-page":"5805","DOI":"10.1128\/IAI.66.12.5805-5811.1998","article-title":"Relationship between hydroxy fatty acids and prostaglandin E2 in gingival tissue","volume":"66","author":"Nichols","year":"1998","journal-title":"Infect. Immun."},{"issue":"6","key":"10.1016\/j.bone.2026.117852_bb0100","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.4049\/jimmunol.1600876","article-title":"TLR tolerance as a treatment for central nervous system autoimmunity","volume":"197","author":"Anstadt","year":"2016","journal-title":"J. Immunol."},{"issue":"12","key":"10.1016\/j.bone.2026.117852_bb0105","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1194\/jlr.M400278-JLR200","article-title":"Structures and biological activity of phosphorylated dihydroceramides of Porphyromonas gingivalis","volume":"45","author":"Nichols","year":"2004","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.bone.2026.117852_bb0110","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"10.1016\/j.bone.2026.117852_bb0115","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0021-9258(19)83961-3","article-title":"Rapid incorporation of phosphate into mitochondrial lipids","volume":"238","author":"Garbus","year":"1968","journal-title":"J. Biol. Chem."},{"issue":"12","key":"10.1016\/j.bone.2026.117852_bb0120","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1194\/jlr.RA120000951","article-title":"A novel phosphoglycerol serine-glycine lipodipeptide of Porphyromonas gingivalis is a TLR2 ligand","volume":"61","author":"Nichols","year":"2020","journal-title":"J. Lipid Res."},{"issue":"4","key":"10.1016\/j.bone.2026.117852_bb0125","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1194\/jlr.M500542-JLR200","article-title":"Structures and biological activities of novel phosphatidylethanolamine lipids of Porphyromonas gingivalis","volume":"47","author":"Nichols","year":"2006","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.bone.2026.117852_bb0130","doi-asserted-by":"crossref","DOI":"10.3389\/froh.2021.698481","article-title":"Serine\/glycine lipid recovery in lipid extracts from healthy and diseased dental samples: relationship to chronic periodontitis","volume":"2","author":"Nichols","year":"2021","journal-title":"Front Oral Health"},{"issue":"4","key":"10.1016\/j.bone.2026.117852_bb0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0151967","article-title":"Differential responses of pattern recognition receptors to outer membrane vesicles of three periodontal pathogens","volume":"11","author":"Cecil","year":"2016","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.bone.2026.117852_bb0140","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0016771","article-title":"Phosphorylated dihydroceramides from common human bacteria are recovered in human tissues","volume":"6","author":"Nichols","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.bone.2026.117852_bb0145","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1415565","article-title":"Microbiome-derived bacterial lipids regulate gene expression of proinflammatory pathway inhibitors in systemic monocytes","volume":"15","author":"Mihori","year":"2024","journal-title":"Front. Immunol."},{"issue":"3","key":"10.1016\/j.bone.2026.117852_bb0150","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.4049\/jimmunol.0900378","article-title":"TLR2-dependent inflammatory response to Porphyromonas gingivalis is MyD88 independent, whereas MyD88 is required to clear infection","volume":"184","author":"Burns","year":"2010","journal-title":"J. Immunol."},{"key":"10.1016\/j.bone.2026.117852_bb0155","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3389\/fcimb.2017.00359","article-title":"Porphyromonas gingivalis stimulates TLR2-PI3K signaling to escape immune clearance and induce bone resorption independently of MyD88","volume":"7","author":"Makkawi","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"4","key":"10.1016\/j.bone.2026.117852_bb0160","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1128\/IAI.01036-12","article-title":"A novel class of lipoprotein lipase-sensitive molecules mediates toll-like receptor 2 activation by Porphyromonas gingivalis","volume":"81","author":"Jain","year":"2013","journal-title":"Infect. Immun."},{"issue":"4","key":"10.1016\/j.bone.2026.117852_bb0165","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1111\/omi.12427","article-title":"Accessory fimbrial subunits and PPAD are necessary for TLR2 activation by Porphyromonas gingivalis","volume":"38","author":"Wielento","year":"2023","journal-title":"Mol Oral Microbiol"},{"issue":"6","key":"10.1016\/j.bone.2026.117852_bb0170","doi-asserted-by":"crossref","first-page":"2430","DOI":"10.2353\/ajpath.2009.090544","article-title":"Unique lipids from a common human bacterium represent a new class of Toll-like receptor 2 ligands capable of enhancing autoimmunity","volume":"175","author":"Nichols","year":"2009","journal-title":"Am. J. Pathol."},{"issue":"8","key":"10.1016\/j.bone.2026.117852_bb0175","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1016\/j.joms.2016.02.016","article-title":"Ultrastructural analyses of alveolar bone in a patient with osteomyelitis secondary to osteopetrosis: a review of the literature","volume":"74","author":"Mikami","year":"2016","journal-title":"J. Oral Maxillofac. Surg."},{"key":"10.1016\/j.bone.2026.117852_bb0180","article-title":"Periodontal profile and radiographic characterization of the jaws in a patient with autosomal dominant osteopetrosis","volume":"2017","author":"da Silva Barbirato","year":"2017","journal-title":"Endocrinol. Diabetes Metab. Case Rep."}],"updated-by":[{"DOI":"10.1016\/j.bone.2026.117946","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000}},{"DOI":"10.1016\/j.bone.2026.117946","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000}}],"container-title":["Bone"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328226000785?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S8756328226000785?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T21:19:50Z","timestamp":1781212790000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S8756328226000785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":36,"alternative-id":["S8756328226000785"],"URL":"https:\/\/doi.org\/10.1016\/j.bone.2026.117852","relation":{},"ISSN":["8756-3282"],"issn-type":[{"value":"8756-3282","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Porphyromonas gingivalis lipids enhance RANKL-mediated osteoclast formation","name":"articletitle","label":"Article Title"},{"value":"Bone","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bone.2026.117852","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117852"}}