{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T00:18:57Z","timestamp":1792887537778,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CMMI 1662284"],"award-info":[{"award-number":["CMMI 1662284"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10RR027668"],"award-info":[{"award-number":["S10RR027668"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD021665"],"award-info":[{"award-number":["S10OD021665"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P01 AG039355"],"award-info":[{"award-number":["P01 AG039355"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01 AR053949"],"award-info":[{"award-number":["R01 AR053949"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bone Reports"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.bonr.2020.100277","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T07:37:30Z","timestamp":1589873850000},"page":"100277","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Osteocyte lacunar strain determination using multiscale finite element analysis"],"prefix":"10.1016","volume":"12","author":[{"given":"Sravan K.","family":"Kola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark T.","family":"Begonia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LeAnn M.","family":"Tiede-Lewis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loretta E.","family":"Laughrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Dallas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark L.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiagarajan","family":"Ganesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bonr.2020.100277_bb0005","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1016\/j.jbiomech.2008.01.031","article-title":"Round versus flat: bone cell morphology, elasticity, and mechanosensing","volume":"41","author":"Bacabac","year":"2008","journal-title":"J. Biomech."},{"key":"10.1016\/j.bonr.2020.100277_bb0010","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1056\/NEJMcp1513724","article-title":"Postmenopausal osteoporosis","volume":"374","author":"Black","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.bonr.2020.100277_bb0015","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-2818.2006.01706.x","article-title":"A guided tour into subcellular colocalization analysis in light microscopy","volume":"224","author":"Bolte","year":"2006","journal-title":"J. Microsc."},{"key":"10.1016\/j.bonr.2020.100277_bb0020","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.bone.2007.12.224","article-title":"Osteocytes, mechanosensing and Wnt signaling","volume":"42","author":"Bonewald","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0025","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1016\/j.jbiomech.2006.10.040","article-title":"Tissue strain amplification at the osteocyte lacuna: a microstructural finite element analysis","volume":"40","author":"Bonivtch","year":"2007","journal-title":"J. Biomech."},{"key":"10.1016\/j.bonr.2020.100277_bb0030","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1359\/jbmr.1997.12.10.1547","article-title":"Muscle strength, bone mass, and age-related bone loss","volume":"12","author":"Burr","year":"1997","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.bonr.2020.100277_bb0035","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/S8756-3282(02)00707-X","article-title":"Effects of biomechanical stress on bones in animals","volume":"30","author":"Burr","year":"2002","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0040","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/BF02406129","article-title":"Mechanical loading histories and cortical bone remodeling","volume":"36","author":"Carter","year":"1984","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.bonr.2020.100277_bb0045","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1115\/1.2891234","article-title":"Candidates for the mechanosensory system in bone","volume":"113","author":"Cowin","year":"1991","journal-title":"J. Biomech. Eng."},{"key":"10.1016\/j.bonr.2020.100277_bb0050","doi-asserted-by":"crossref","DOI":"10.1038\/boneres.2016.32","article-title":"A multiscale 3D finite element analysis of fluid\/solute transport in mechanically loaded bone","volume":"4","author":"Fan","year":"2016","journal-title":"Bone Research"},{"key":"10.1016\/j.bonr.2020.100277_bb0055","doi-asserted-by":"crossref","first-page":"E419","DOI":"10.1152\/ajpendo.1996.270.3.E419","article-title":"Increased bone formation in rat tibiae after a single short period of dynamic loading in vivo","volume":"270","author":"Forwood","year":"1996","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.bonr.2020.100277_bb0060","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.medengphy.2010.02.020","article-title":"A subject specific multibody model of the knee with menisci","volume":"32","author":"Guess","year":"2010","journal-title":"Med. Eng. Phys."},{"key":"10.1016\/j.bonr.2020.100277_bb0065","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.bone.2018.01.018","article-title":"A new open-source tool for measuring 3D osteocyte lacunar geometries from confocal laser scanning microscopy reveals age-related changes to lacunar size and shape in cortical mouse bone","volume":"110","author":"Heveran","year":"2018","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0070","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1002\/jbmr.2900","article-title":"Activation of Wnt signaling by mechanical loading is impaired in the bone of old mice","volume":"31","author":"Holguin","year":"2016","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.bonr.2020.100277_bb0075","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1096\/fj.10-157370","article-title":"Mechanotransduction in bone repair and regeneration","volume":"24","author":"Huang","year":"2010","journal-title":"FASEB J."},{"key":"10.1016\/j.bonr.2020.100277_bb0080","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cmpb.2016.05.019","article-title":"Numerical simulation of osteocyte cell in response to directional mechanical loadings and mechanotransduction analysis: considering lacunar\u2013canalicular interstitial fluid flow","volume":"133","author":"Joukar","year":"2016","journal-title":"Comput. Methods Prog. Biomed."},{"key":"10.1016\/j.bonr.2020.100277_bb0085","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.bone.2015.02.011","article-title":"Novel approaches for two and three dimensional multiplexed imaging of osteocytes","volume":"76","author":"Kamel-ElSayed","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0090","doi-asserted-by":"crossref","first-page":"S102","DOI":"10.1007\/BF01673415","article-title":"Osteocytes, strain detection, bone modeling and remodeling","volume":"53","author":"Lanyon","year":"1993","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.bonr.2020.100277_bb0095","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.bone.2015.03.019","article-title":"In vivo mechanical loading rapidly activates \u03b2-catenin signaling in osteocytes through a prostaglandin mediated mechanism","volume":"76","author":"Lara-Castillo","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0100","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.bone.2015.03.019","article-title":"In vivo mechanical loading rapidly activates \u03b2-catenin signaling in osteocytes through a prostaglandin mediated mechanism","volume":"76","author":"Lara-Castillo","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0105","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.medengphy.2011.07.022","article-title":"Load\/strain distribution between ulna and radius in the mouse forearm compression loading model","volume":"34","author":"Lu","year":"2012","journal-title":"Med. Eng. Phys."},{"key":"10.1016\/j.bonr.2020.100277_bb0110","series-title":"FEBio Theory Manual","author":"Maas","year":"2011"},{"key":"10.1016\/j.bonr.2020.100277_bb0115","doi-asserted-by":"crossref","DOI":"10.1115\/1.4005694","article-title":"FEBio: finite elements for biomechanics","volume":"134","author":"Maas","year":"2012","journal-title":"J. Biomech. Eng."},{"key":"10.1016\/j.bonr.2020.100277_bb0120","doi-asserted-by":"crossref","unstructured":"Mader KS, Schneider P, M\u00fcller R, Stampanoni M. A quantitative framework for the 3D characterization of the osteocyte lacunar system. Bone 57: 142\u2013154.","DOI":"10.1016\/j.bone.2013.06.026"},{"key":"10.1016\/j.bonr.2020.100277_bb0125","unstructured":"Materialise P, MI. MIMICS Innovation Suite. In."},{"key":"10.1016\/j.bonr.2020.100277_bb0130","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1080\/01495739708936695","article-title":"Strain concentrations surrounding an ellipsoid model of lacunae and osteocytes","volume":"1","author":"McCreadie","year":"1997","journal-title":"Computer Methods in Biomechanics and Biomedical Engineering"},{"key":"10.1016\/j.bonr.2020.100277_bb0135","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1007\/s00198-011-1656-4","article-title":"Mechanical loading-related changes in osteocyte sclerostin expression in mice are more closely associated with the subsequent osteogenic response than the peak strains engendered","volume":"23","author":"Moustafa","year":"2012","journal-title":"Osteoporos. Int."},{"key":"10.1016\/j.bonr.2020.100277_bb0140","first-page":"261","article-title":"Microstructural strain near osteocyte lacuna in cortical bone in vitro","volume":"2","author":"Nicolella","year":"2002","journal-title":"J. Musculoskelet. Nueronal Interact."},{"key":"10.1016\/j.bonr.2020.100277_bb0145","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1016\/j.jbiomech.2005.04.032","article-title":"Osteocyte lacunae tissue strain in cortical bone","volume":"39","author":"Nicolella","year":"2006","journal-title":"J. Biomech."},{"key":"10.1016\/j.bonr.2020.100277_bb0150","first-page":"330","article-title":"Effects of nanomechanical bone tissue properties on bone tissue strain: implications for osteocyte mechanotransduction","volume":"8","author":"Nicolella","year":"2008","journal-title":"J. Musculoskelet. Neuronal Interact."},{"key":"10.1016\/j.bonr.2020.100277_bb0155","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/ajpa.20155","article-title":"The aging of Wolff\u2019s \u201claw\u201d: ontogeny and responses to mechanical loading in cortical bone","volume":"125","author":"Pearson","year":"2004","journal-title":"Am. J. Phys. Anthropol."},{"key":"10.1016\/j.bonr.2020.100277_bb0160","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1002\/jbmr.1567","article-title":"Demonstration of osteocytic perilacunar\/canalicular remodeling in mice during lactation","volume":"27","author":"Qing","year":"2012","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.bonr.2020.100277_bb0165","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S1350-4533(98)00007-1","article-title":"Mechanical properties and the hierarchical structure of bone","volume":"20","author":"Rho","year":"1998","journal-title":"Med. Eng. Phys."},{"key":"10.1016\/j.bonr.2020.100277_bb0170","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/S8756-3282(02)00871-2","article-title":"Mechanotransduction in bone: genetic effects on mechanosensitivity in mice","volume":"31","author":"Robling","year":"2002","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0175","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s00198-010-1194-5","article-title":"Osteocyte: the unrecognized side of bone tissue","volume":"21","author":"Rochefort","year":"2010","journal-title":"Osteoporos. Int."},{"key":"10.1016\/j.bonr.2020.100277_bb0180","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1007\/s00198-009-0858-5","article-title":"The role of osteocytes in bone mechanotransduction","volume":"20","author":"Santos","year":"2009","journal-title":"Osteoporos. Int."},{"key":"10.1016\/j.bonr.2020.100277_bb0185","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.bone.2013.01.037","article-title":"Measurement and estimation of osteocyte mechanical strain","volume":"54","author":"Stern","year":"2013","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0190","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bone.2008.02.020","article-title":"The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes","volume":"43","author":"Sugawara","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0195","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.bone.2012.09.033","article-title":"The early mouse 3D osteocyte network in the presence and absence of mechanical loading","volume":"52","author":"Sugawara","year":"2013","journal-title":"Bone"},{"issue":"10","key":"10.1016\/j.bonr.2020.100277_bb0200","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.18632\/aging.101308","article-title":"Degeneration of the osteocyte network in the C57BL\/6 mouse model of aging","volume":"9","author":"Tiede-Lewis","year":"2017","journal-title":"Aging"},{"key":"10.1016\/j.bonr.2020.100277_bb0205","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/8756-3282(92)90084-A","article-title":"Structural changes in rat bone subjected to long-term, in vivo mechanical loading","volume":"13","author":"Turner","year":"1992","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0210","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.bone.2009.04.238","article-title":"Osteocyte morphology in human tibiae of different bone pathologies with different bone mineral density\u2014is there a role for mechanosensing?","volume":"45","author":"van Hove","year":"2009","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0215","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.bone.2008.01.030","article-title":"Osteocyte morphology in fibula and calvaria\u2014is there a role for mechanosensing?","volume":"43","author":"Vatsa","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.bonr.2020.100277_bb0220","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1098\/rsif.2012.0286","article-title":"Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes","volume":"9","author":"Verbruggen","year":"2012","journal-title":"J. R. Soc. Interface"},{"key":"10.1016\/j.bonr.2020.100277_bb0225","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s10237-013-0487-y","article-title":"Fluid flow in the osteocyte mechanical environment: a fluid\u2013structure interaction approach","volume":"13","author":"Verbruggen","year":"2014","journal-title":"Biomech. Model. Mechanobiol."}],"updated-by":[{"DOI":"10.1016\/j.bonr.2021.101084","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000}}],"container-title":["Bone Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352187220300371?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352187220300371?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:08:42Z","timestamp":1778872122000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352187220300371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":45,"alternative-id":["S2352187220300371"],"URL":"https:\/\/doi.org\/10.1016\/j.bonr.2020.100277","relation":{},"ISSN":["2352-1872"],"issn-type":[{"value":"2352-1872","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Osteocyte lacunar strain determination using multiscale finite element analysis","name":"articletitle","label":"Article Title"},{"value":"Bone Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bonr.2020.100277","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"100277"}}