{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:25:02Z","timestamp":1790832302183,"version":"4.1.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":1202,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2010,4]]},"DOI":"10.1016\/j.bpj.2009.12.4298","type":"journal-article","created":{"date-parts":[[2010,4,19]],"date-time":"2010-04-19T04:48:59Z","timestamp":1271652539000},"page":"1626-1631","source":"Crossref","is-referenced-by-count":110,"title":["The Behavior of the Hydrophobic Effect under Pressure and Protein Denaturation"],"prefix":"10.1016","volume":"98","author":[{"given":"J. Ra\u00fal","family":"Grigera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres N.","family":"McCarthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2009.12.4298_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-3233(08)60608-7","article-title":"Some factors in the interpretation of the protein denaturation","volume":"14","author":"Kauzmann","year":"1959","journal-title":"Adv. Protein Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib2","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1021\/bi00227a001","article-title":"Protein folding: local structures, domains, subunits, and assemblies","volume":"30","author":"Jaenicke","year":"1991","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2009.12.4298_bib3","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0167-4838(01)00344-2","article-title":"Revisiting volume changes in pressure-induced protein unfolding","volume":"1595","author":"Royer","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.bpj.2009.12.4298_bib4","doi-asserted-by":"crossref","first-page":"3734","DOI":"10.1021\/j100715a008","article-title":"Molecular motion and structure of aqueous mixtures with nonelectrolytes as studied by nuclear magnetic relaxation methods","volume":"74","author":"Goldammer","year":"1970","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib5","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1021\/j100456a009","article-title":"Influence of hydrophobic solutes on the dynamic behavior of water","volume":"84","author":"Hallenga","year":"1980","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib6","doi-asserted-by":"crossref","first-page":"148301","DOI":"10.1103\/PhysRevLett.99.148301","article-title":"Observation of immobilized water molecules around hydrophobic groups","volume":"99","author":"Rezus","year":"2007","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2009.12.4298_bib7","series-title":"Water: A Matrix of Life","author":"Franks","year":"2000"},{"key":"10.1016\/j.bpj.2009.12.4298_bib8","volume":"Vol. 4","year":"1980"},{"key":"10.1016\/j.bpj.2009.12.4298_bib9","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1039\/f19757100784","article-title":"Tracer diffusion of tritiated water (THO) in ordinary water (H2O) under pressure","volume":"71","author":"Woolf","year":"1975","journal-title":"J. C. S. Faraday I."},{"key":"10.1016\/j.bpj.2009.12.4298_bib10","first-page":"1084","article-title":"Structure of supercooled and glassy water under pressure","volume":"60","author":"Starr","year":"1999","journal-title":"Phys. Rev. E. Stat. Phys. Plasmas Fluids Relat. Interdiscip. Topics."},{"key":"10.1016\/j.bpj.2009.12.4298_bib11","doi-asserted-by":"crossref","first-page":"025502","DOI":"10.1103\/PhysRevLett.94.025502","article-title":"Ordering of hydrogen bonds in high-pressure low-temperature H2O","volume":"94","author":"Cai","year":"2005","journal-title":"Phys. Rev Lett."},{"key":"10.1016\/j.bpj.2009.12.4298_bib12","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1021\/j100879a018","article-title":"The effect of electrolyte on the viscosity of water under pressure","volume":"70","author":"Horne","year":"1966","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib13","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1103\/PhysRevLett.59.1128","article-title":"Diffusion in supercooled water to 300 MPa","volume":"59","author":"Prielmeier","year":"1987","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2009.12.4298_bib14","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1021\/bi00789a002","article-title":"Reversible pressure\u2014temperature denaturation of chymotrypsinogen","volume":"10","author":"Hawley","year":"1971","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2009.12.4298_bib15","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0167-4838(01)00332-6","article-title":"Pressure-temperature phase diagrams of biomolecules","volume":"1595","author":"Smeller","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2009.12.4298_bib16","doi-asserted-by":"crossref","first-page":"5756","DOI":"10.1073\/pnas.0710409105","article-title":"Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram","volume":"105","author":"Wiedersich","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2009.12.4298_bib17","doi-asserted-by":"crossref","first-page":"138103","DOI":"10.1103\/PhysRevLett.91.138103","article-title":"Possible mechanism for cold denaturation of proteins at high pressure","volume":"91","author":"Marqu\u00e9s","year":"2003","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2009.12.4298_bib18","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1073\/pnas.95.4.1552","article-title":"The pressure dependence of hydrophobic interactions is consistent with the observed pressure denaturation of proteins","volume":"95","author":"Hummer","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2009.12.4298_bib19","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/prot.340210302","article-title":"Funnels, pathways, and the energy landscape of protein folding: a synthesis","volume":"21","author":"Bryngelson","year":"1995","journal-title":"Proteins"},{"key":"10.1016\/j.bpj.2009.12.4298_bib20","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s10867-008-9083-2","article-title":"Studying the unfolding kinetics of proteins under pressure using long molecular dynamic simulation runs","volume":"33","author":"Chara","year":"2007","journal-title":"J. Biol. Phys."},{"key":"10.1016\/j.bpj.2009.12.4298_bib21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0010-4655(95)00042-E","article-title":"GROMACS: a message-passing parallel molecular dynamics implementation","volume":"91","author":"Berendsen","year":"1995","journal-title":"Comput. Phys. Commun."},{"key":"10.1016\/j.bpj.2009.12.4298_bib22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s008940100045","article-title":"GROMACS 3.0: a package for molecular simulation and trajectory analysis","volume":"7","author":"Lindahl","year":"2001","journal-title":"J. Mol. Model."},{"key":"10.1016\/j.bpj.2009.12.4298_bib23","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1002\/anie.199009921","article-title":"Computer simulation of molecular dynamics: methodology, applications, and perspectives in chemistry","volume":"29","author":"van Gunsteren","year":"1990","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bpj.2009.12.4298_bib24","series-title":"GROMOS-87 Manual","author":"van Gunsteren","year":"1987"},{"key":"10.1016\/j.bpj.2009.12.4298_bib25","doi-asserted-by":"crossref","first-page":"9206","DOI":"10.1021\/j100138a023","article-title":"A molecular dynamics study of the decane\/water interface","volume":"97","author":"Buuren","year":"1993","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib26","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H","article-title":"LINCS: a linear constraint solver for molecular simulations","volume":"18","author":"Hess","year":"1997","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib27","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/jcc.540130805","article-title":"SETTLE: an analytical version of the SHAKE and RATTLE algorithms for rigid water models","volume":"13","author":"Miyamoto","year":"1992","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib28","doi-asserted-by":"crossref","first-page":"6269","DOI":"10.1021\/j100308a038","article-title":"The missing term in effective pair potentials","volume":"91","author":"Berendsen","year":"1987","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib29","doi-asserted-by":"crossref","first-page":"17616","DOI":"10.1021\/jp0641029","article-title":"Hydration thermodynamic properties of amino acid analogues: a systematic comparison of biomolecular force fields and water models","volume":"110","author":"Hess","year":"2006","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bpj.2009.12.4298_bib30","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.pepi.2004.11.003","article-title":"Prediction of the PVT properties of water over wide range of temperatures and pressures from molecular dynamics simulation","volume":"149","author":"Zhang","year":"2005","journal-title":"Phys. Earth Planet. Inter."},{"key":"10.1016\/j.bpj.2009.12.4298_bib31","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1063\/1.448118","article-title":"Molecular dynamics with coupling to an external bath","volume":"81","author":"Berendsen","year":"1984","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.bpj.2009.12.4298_bib32","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1038\/366668a0","article-title":"The interatomic structure of water at supercritical temperatures","volume":"366","author":"Postorino","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2009.12.4298_bib33","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/35053024","article-title":"Relationship between structural order and the anomalies of liquid water","volume":"409","author":"Errington","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2009.12.4298_bib34","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/378364a0","article-title":"Effect of high salt concentrations on water structure","volume":"378","author":"Leberman","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2009.12.4298_bib35","doi-asserted-by":"crossref","first-page":"104502","DOI":"10.1063\/1.2768966","article-title":"Clustering of Lennard-Jones particles in water: temperature and pressure effects","volume":"127:","author":"Ferrara","year":"2007","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.bpj.2009.12.4298_bib36","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.jmgm.2005.09.007","article-title":"Effect of pressure on the conformation of proteins. A molecular dynamics simulation of lysozyme","volume":"24","author":"McCarthy","year":"2006","journal-title":"J. Mol. Graph. Model."},{"key":"10.1016\/j.bpj.2009.12.4298_bib37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/jcc.540160303","article-title":"The double cube lattice method: efficient approaches to numerical integration of surface area and volume and to dot surface contouring of molecular assemblies","volume":"16","author":"Eisenhaber","year":"1995","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.bpj.2009.12.4298_bib38","series-title":"Dynamics of Proteins and Nucleic Acids","author":"McCammon","year":"1987"},{"key":"10.1016\/j.bpj.2009.12.4298_bib39","doi-asserted-by":"crossref","first-page":"10875","DOI":"10.1021\/bi034722p","article-title":"Highly fluctuating protein structures revealed by\u00a0variable-pressure nuclear magnetic resonance","volume":"42","author":"Akasaka","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2009.12.4298_bib40","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0022-2836(02)00449-7","article-title":"High pressure NMR reveals that apomyoglobin is an equilibrium mixture from the native to the unfolded","volume":"320","author":"Kitahara","year":"2002","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2009.12.4298_bib41","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.bbapap.2005.11.015","article-title":"Pressure denaturation of apomyoglobin: a molecular dynamics simulation study","volume":"1764","author":"McCarthy","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2009.12.4298_bib42","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/325763a0","article-title":"Thermodynamics of unfolding","volume":"325","author":"Kauzmann","year":"1987","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2009.12.4298_bib43","doi-asserted-by":"crossref","first-page":"094509","DOI":"10.1063\/1.1854626","article-title":"Hydrophobic effect on partial molar volume","volume":"122","author":"Imai","year":"2005","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.bpj.2009.12.4298_bib44","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.bbapap.2006.01.003","article-title":"A molecular dynamics simulation of SNASE and its hydration shell at high temperature and high pressure","volume":"1764","author":"Smolin","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2009.12.4298_bib45","doi-asserted-by":"crossref","first-page":"037803","DOI":"10.1103\/PhysRevLett.103.037803","article-title":"Quantifying water density fluctuations and compressibility of hydration shells of hydrophobic solutes and proteins","volume":"103","author":"Sarupria","year":"2009","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2009.12.4298_bib46","doi-asserted-by":"crossref","first-page":"17754","DOI":"10.1073\/pnas.0804775105","article-title":"Computing the stability diagram of the Trp- Cage miniprotein","volume":"118","author":"Paschek","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2009.12.4298_bib47","doi-asserted-by":"crossref","first-page":"5961","DOI":"10.1021\/jp075928t","article-title":"Mechanistic elements of protein cold denaturation","volume":"112","author":"Lopez","year":"2008","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bpj.2009.12.4298_bib48","doi-asserted-by":"crossref","first-page":"8907","DOI":"10.1002\/anie.200702736","article-title":"Pressure and salt effects in simulated water: two sides of the same coin?","volume":"46","author":"Holzmann","year":"2007","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.bpj.2009.12.4298_bib49","doi-asserted-by":"crossref","first-page":"9877","DOI":"10.1021\/bi980894o","article-title":"Local dynamics of apocytochrome b562 examined by the dependence of hydrogen exchange on hydration pressure","volume":"47","author":"Fuentes","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2009.12.4298_bib50","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/S0022-2836(03)00209-2","article-title":"Pressure-dependent changes in the solution structure of hen egg-white lysozyme","volume":"327","author":"Refaee","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2009.12.4298_bib51","first-page":"5952","article-title":"Pressure versus heat-induced unfolding of ribonuclease A: the case of hydrophobic interactions within a chain-folding initiation site","volume":"42","author":"Torrent","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2009.12.4298_bib52","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1002\/prot.1128","article-title":"Filling a cavity dramatically increases pressure stability of the c-Myb R2 subdomain","volume":"45","author":"Lassalle","year":"2001","journal-title":"Proteins"},{"key":"10.1016\/j.bpj.2009.12.4298_bib53","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1006\/fstl.1996.0118","article-title":"Mechanism of high pressure denaturation of proteins","volume":"29","author":"Hayakawa","year":"1996","journal-title":"Lebensm. Wiss. Technol."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349509061505?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349509061505?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T14:55:57Z","timestamp":1544540157000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349509061505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":53,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["S0006349509061505"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2009.12.4298","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}