{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:31:06Z","timestamp":1762101066713},"reference-count":56,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T00:00:00Z","timestamp":1376697600000},"content-version":"vor","delay-in-days":1143,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2010,7]]},"DOI":"10.1016\/j.bpj.2010.04.061","type":"journal-article","created":{"date-parts":[[2010,7,21]],"date-time":"2010-07-21T08:22:29Z","timestamp":1279700549000},"page":"638-646","source":"Crossref","is-referenced-by-count":27,"title":["Structure and Orientation of a Voltage-Sensor Toxin in Lipid Membranes"],"prefix":"10.1016","volume":"99","author":[{"given":"Hyun Ho","family":"Jung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoi Jong","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirela","family":"Milescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chul Won","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seungkyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Yeon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Jae","family":"Eu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha Hyung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenton J.","family":"Swartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Il","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2010.04.061_bib1","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/nature07620","article-title":"Sensing voltage across lipid membranes","volume":"456","author":"Swartz","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib2","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nature06265","article-title":"Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment","volume":"450","author":"Long","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib3","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1126\/science.1116269","article-title":"Crystal structure of a mammalian voltage-dependent Shaker family K+ channel","volume":"309","author":"Long","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.bpj.2010.04.061_bib4","doi-asserted-by":"crossref","first-page":"15441","DOI":"10.1073\/pnas.0507651102","article-title":"Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane","volume":"102","author":"Lee","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2010.04.061_bib5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/nature01580","article-title":"X-ray structure of a voltage-dependent K+ channel","volume":"423","author":"Jiang","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib6","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.cell.2005.08.041","article-title":"Calibrated measurement of gating-charge arginine displacement in the KvAP voltage-dependent K+ channel","volume":"123","author":"Ruta","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.bpj.2010.04.061_bib7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/nature01581","article-title":"The principle of gating charge movement in a voltage-dependent K+ channel","volume":"423","author":"Jiang","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib8","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1126\/science.1101373","article-title":"Molecular architecture of the KvAP voltage-dependent K+ channel in a lipid bilayer","volume":"306","author":"Cuello","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.bpj.2010.04.061_bib9","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.str.2007.12.015","article-title":"Structural dynamics of an isolated voltage-sensor domain in a lipid bilayer","volume":"16","author":"Chakrapani","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.bpj.2010.04.061_bib10","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nature05416","article-title":"Phospholipids and the origin of cationic gating charges in voltage sensors","volume":"444","author":"Schmidt","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib11","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/nature04880","article-title":"Enzymatic activation of voltage-gated potassium channels","volume":"442","author":"Ramu","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib12","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1038\/nature06266","article-title":"Portability of paddle motif function and pharmacology in voltage sensors","volume":"450","author":"Alabi","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib13","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1085\/jgp.200709869","article-title":"Tarantula toxins interact with voltage sensors within lipid membranes","volume":"130","author":"Milescu","year":"2007","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib14","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1038\/nature06618","article-title":"Removal of phospho-head groups of membrane lipids immobilizes voltage sensors of K+ channels","volume":"451","author":"Xu","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib15","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/nature07473","article-title":"Deconstructing voltage sensor function and pharmacology in sodium channels","volume":"456","author":"Bosmans","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib16","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.jmb.2008.06.010","article-title":"Inferred motions of the S3a helix during voltage-dependent K+ channel gating","volume":"381","author":"Banerjee","year":"2008","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib17","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1038\/nsmb.1679","article-title":"Interactions between lipids and voltage sensor paddles detected with tarantula toxins","volume":"16","author":"Milescu","year":"2009","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib18","doi-asserted-by":"crossref","first-page":"15950","DOI":"10.1074\/jbc.271.27.15950","article-title":"Molecular determinants of high affinity binding of \u03b1-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel \u03b1 subunit","volume":"271","author":"Rogers","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bpj.2010.04.061_bib19","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S0896-6273(00)80306-2","article-title":"Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites","volume":"18","author":"Swartz","year":"1997","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2010.04.061_bib20","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/S0896-6273(00)80307-4","article-title":"Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels","volume":"18","author":"Swartz","year":"1997","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2010.04.061_bib21","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/S0896-6273(00)80606-6","article-title":"Voltage sensor-trapping: enhanced activation of sodium channels by \u03b2-scorpion toxin bound to the S3-S4 loop in domain II","volume":"21","author":"Cest\u00e8le","year":"1998","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2010.04.061_bib22","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1085\/jgp.115.6.673","article-title":"Localization and molecular determinants of the Hanatoxin receptors on the voltage-sensing domains of a K+ channel","volume":"115","author":"Li-Smerin","year":"2000","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib23","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/S0300-9084(00)01174-3","article-title":"Molecular mechanisms of neurotoxin action on voltage-gated sodium channels","volume":"82","author":"Cest\u00e8le","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.bpj.2010.04.061_bib24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1085\/jgp.117.3.205","article-title":"Helical structure of the COOH terminus of S3 and its contribution to the gating modifier toxin receptor in voltage-gated ion channels","volume":"117","author":"Li-Smerin","year":"2001","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib25","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0896-6273(03)00636-6","article-title":"Interaction between extracellular Hanatoxin and the resting conformation of the voltage-sensor paddle in Kv channels","volume":"40","author":"Lee","year":"2003","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2010.04.061_bib26","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/nature03873","article-title":"Voltage-sensor activation with a tarantula toxin as cargo","volume":"436","author":"Phillips","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib27","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nature02632","article-title":"A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom","volume":"430","author":"Lee","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib28","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/nature02743","article-title":"Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers","volume":"430","author":"Suchyna","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2010.04.061_bib29","doi-asserted-by":"crossref","first-page":"6015","DOI":"10.1021\/bi0477034","article-title":"Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel","volume":"44","author":"Jung","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.04.061_bib30","doi-asserted-by":"crossref","first-page":"11127","DOI":"10.1074\/jbc.M412552200","article-title":"Differential phospholipid binding by site 3 and site 4 toxins. Implications for structural variability between voltage-sensitive sodium channel domains","volume":"280","author":"Smith","year":"2005","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2010.04.061_bib31","doi-asserted-by":"crossref","first-page":"19276","DOI":"10.1073\/pnas.0810187105","article-title":"Voltage-dependent K+ channel gating and voltage sensor toxin sensitivity depend on the mechanical state of the lipid membrane","volume":"105","author":"Schmidt","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2010.04.061_bib32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1006\/jmbi.2000.3609","article-title":"Solution structure of hanatoxin1, a gating modifier of voltage-dependent K+ channels: common surface features of gating modifier toxins","volume":"297","author":"Takahashi","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib33","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0022-2836(02)00595-8","article-title":"Solution structure of \u03c9-grammotoxin SIA, a gating modifier of P\/Q and N-type Ca2+ channel","volume":"321","author":"Takeuchi","year":"2002","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib34","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1021\/bi0353373","article-title":"Solution structure and functional characterization of SGTx1, a modifier of Kv2.1 channel gating","volume":"43","author":"Lee","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.04.061_bib35","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1046\/j.1432-1327.1999.00726.x","article-title":"Isolation, amino acid sequence and functional assays of SGTx1. The first toxin purified from the venom of the spider Scodra griseipes","volume":"265","author":"Marvin","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bpj.2010.04.061_bib36","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1085\/jgp.200309005","article-title":"Molecular surface of tarantula toxins interacting with voltage sensors in K(v) channels","volume":"123","author":"Wang","year":"2004","journal-title":"J.\u00a0Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib37","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/73331","article-title":"A novel NMR method for determining the interfaces of large protein-protein complexes","volume":"7","author":"Takahashi","year":"2000","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib38","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0022-2836(02)00018-9","article-title":"Determination of the interface of a large protein complex by transferred cross-saturation measurements","volume":"318","author":"Nakanishi","year":"2002","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib39","doi-asserted-by":"crossref","first-page":"4981","DOI":"10.1074\/jbc.M307815200","article-title":"Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR","volume":"279","author":"Takeuchi","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bpj.2010.04.061_bib40","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1006\/abio.2000.4773","article-title":"How to measure and analyze tryptophan fluorescence in membranes properly, and why bother?","volume":"285","author":"Ladokhin","year":"2000","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bpj.2010.04.061_bib41","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/S0076-6879(97)78024-8","article-title":"Distribution analysis of depth-dependent fluorescence quenching in membranes: a practical guide","volume":"278","author":"Ladokhin","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bpj.2010.04.061_bib42","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/S0006-3495(99)77258-9","article-title":"Analysis of protein and peptide penetration into membranes by depth-dependent fluorescence quenching: theoretical considerations","volume":"76","author":"Ladokhin","year":"1999","journal-title":"Biophys. J."},{"key":"10.1016\/j.bpj.2010.04.061_bib43","doi-asserted-by":"crossref","first-page":"5312","DOI":"10.1021\/bi00138a010","article-title":"Calibration of the parallax fluorescence quenching method for determination of membrane penetration depth: refinement and comparison of quenching by spin-labeled and brominated lipids","volume":"31","author":"Abrams","year":"1992","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.04.061_bib44","doi-asserted-by":"crossref","first-page":"10826","DOI":"10.1021\/bi00091a038","article-title":"Extension of the parallax analysis of membrane penetration depth to the polar region of model membranes: use of fluorescence quenching by a spin-label attached to the phospholipid polar headgroup","volume":"32","author":"Abrams","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.04.061_bib45","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.jmb.2004.11.026","article-title":"The solution structure of the C-terminal domain of TonB and interaction studies with TonB box peptides","volume":"345","author":"Sean Peacock","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib46","doi-asserted-by":"crossref","first-page":"35494","DOI":"10.1074\/jbc.M311520200","article-title":"Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-\u03b1","volume":"279","author":"Weljie","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bpj.2010.04.061_bib47","series-title":"SPARKY 3","author":"Goddard","year":"2004"},{"key":"10.1016\/j.bpj.2010.04.061_bib48","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1006\/jmbi.1997.1291","article-title":"Solution structure of the first three zinc fingers of TFIIIA bound to the cognate DNA sequence: determinants of affinity and sequence specificity","volume":"273","author":"Wuttke","year":"1997","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib49","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nsb0897-605","article-title":"Domain packing and dynamics in the DNA complex of the N-terminal zinc fingers of TFIIIA","volume":"4","author":"Foster","year":"1997","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.bpj.2010.04.061_bib50","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1006\/jmre.2001.2499","article-title":"Complete relaxation and conformational exchange matrix (CORCEMA) analysis of intermolecular saturation transfer effects in reversibly forming ligand-receptor complexes","volume":"155","author":"Jayalakshmi","year":"2002","journal-title":"J. Magn. Reson."},{"key":"10.1016\/j.bpj.2010.04.061_bib51","doi-asserted-by":"crossref","first-page":"L07","DOI":"10.1529\/biophysj.106.098681","article-title":"SGTx1, a Kv channel gating-modifier toxin, binds to the interfacial region of lipid bilayers","volume":"92","author":"Wee","year":"2007","journal-title":"Biophys. J."},{"key":"10.1016\/j.bpj.2010.04.061_bib52","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1085\/jgp.20028636","article-title":"Movement and crevices around a sodium channel S3 segment","volume":"120","author":"Nguyen","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib53","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0896-6273(03)00646-9","article-title":"The orientation and molecular movement of a K+ channel voltage-sensing domain","volume":"40","author":"Gandhi","year":"2003","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2010.04.061_bib54","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1085\/jgp.200609624","article-title":"Constraints on voltage sensor movement in the shaker K+ channel","volume":"128","author":"Darman","year":"2006","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.bpj.2010.04.061_bib55","doi-asserted-by":"crossref","first-page":"34443","DOI":"10.1074\/jbc.M202715200","article-title":"Solution structure of peptide toxins that block mechanosensitive ion channels","volume":"277","author":"Oswald","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bpj.2010.04.061_bib56","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1085\/jgp.115.5.583","article-title":"Identification of a peptide toxin from Grammostola spatulata spider venom that blocks\u00a0cation-selective stretch-activated channels","volume":"115","author":"Suchyna","year":"2000","journal-title":"J. Gen. Physiol."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000634951000559X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000634951000559X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,9]],"date-time":"2018-12-09T12:29:58Z","timestamp":1544358598000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000634951000559X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["S000634951000559X"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2010.04.061","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}