{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:30:39Z","timestamp":1788870639500,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":1019,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB 054652"],"award-info":[{"award-number":["MCB 054652"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM069557"],"award-info":[{"award-number":["GM069557"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2010,10]]},"DOI":"10.1016\/j.bpj.2010.08.005","type":"journal-article","created":{"date-parts":[[2010,10,6]],"date-time":"2010-10-06T04:04:57Z","timestamp":1286337897000},"page":"2180-2189","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Nucleotide-Independent Nitroxide Probe Reports on Site-Specific Stereomeric Environment in DNA"],"prefix":"10.1016","volume":"99","author":[{"given":"Anna M.","family":"Popova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Z.","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2010.08.005_bib1","doi-asserted-by":"crossref","first-page":"7806","DOI":"10.1021\/bi00445a042","article-title":"Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines","volume":"28","author":"Altenbach","year":"1989","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.08.005_bib2","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.sbi.2006.08.008","article-title":"Recent advances and applications of site-directed spin labeling","volume":"16","author":"Fanucci","year":"2006","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.bpj.2010.08.005_bib3","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0079-6603(08)00005-6","article-title":"Site-directed spin labeling studies on nucleic acid structure and dynamics","volume":"82","author":"Sowa","year":"2008","journal-title":"Prog. Nucleic Acids Res. Mol. Biol."},{"key":"10.1016\/j.bpj.2010.08.005_bib4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0076-6879(09)69015-7","article-title":"Studying RNA using site-directed spin-labeling and continuous-wave electron paramagnetic resonance spectroscopy","volume":"469","author":"Zhang","year":"2009","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bpj.2010.08.005_bib5","doi-asserted-by":"crossref","first-page":"6929","DOI":"10.1021\/bi010294g","article-title":"Quantitative analysis of the isolated GAAA tetraloop\/receptor interaction in solution: a site-directed spin labeling study","volume":"40","author":"Qin","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.08.005_bib6","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1038\/nprot.2007.308","article-title":"Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe","volume":"2","author":"Qin","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.bpj.2010.08.005_bib7","doi-asserted-by":"crossref","first-page":"8540","DOI":"10.1021\/bi900860w","article-title":"Site-specific DNA structural and dynamic features revealed by nucleotide-independent nitroxide probes","volume":"48","author":"Popova","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.08.005_bib8","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1021\/ja808217s","article-title":"Motions of the substrate recognition duplex in a group I intron assessed by site-directed spin labeling","volume":"131","author":"Grant","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bpj.2010.08.005_bib9","doi-asserted-by":"crossref","first-page":"4722","DOI":"10.1093\/nar\/gkl546","article-title":"Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe","volume":"34","author":"Cai","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bpj.2010.08.005_bib10","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1529\/biophysj.107.109439","article-title":"Nanometer distance measurements in RNA using site-directed spin labeling","volume":"93","author":"Cai","year":"2007","journal-title":"Biophys. J."},{"key":"10.1016\/j.bpj.2010.08.005_bib11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.bbrc.2008.04.088","article-title":"Diastereomer characterizations of nitroxide-labeled nucleic acids","volume":"371","author":"Grant","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bpj.2010.08.005_bib12","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/bip.20769","article-title":"Computation of nitroxide-nitroxide distances in spin-labeled DNA duplexes","volume":"87","author":"Price","year":"2007","journal-title":"Biopolymers."},{"key":"10.1016\/j.bpj.2010.08.005_bib13","doi-asserted-by":"crossref","first-page":"4759","DOI":"10.1093\/nar\/27.24.4759","article-title":"An NMR and molecular modelling analysis of d(CTACTGCTTTAG). d(CTAAAGCAGTAG) reveals that the particular behaviour of TpA steps is related to edge-to-edge contacts of their base-pairs in the major groove","volume":"27","author":"Leporc","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bpj.2010.08.005_bib14","series-title":"Advanced ESR Methods in\u00a0Polymer Reaserch","first-page":"53","article-title":"Calculating slow-motion ESR\u00a0spectra of spin-labeled polymers","author":"Earle","year":"2006"},{"key":"10.1016\/j.bpj.2010.08.005_bib15","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1080\/07328319608002393","article-title":"Isolation of oligodeoxynucleoside phosphorothioate diastereomers by the combination of DEAE ion-exchange and reversed-phase chromatography","volume":"15","author":"Kanehara","year":"1996","journal-title":"Nucleosides Nucleotides"},{"key":"10.1016\/j.bpj.2010.08.005_bib16","doi-asserted-by":"crossref","first-page":"8855","DOI":"10.1074\/jbc.271.15.8855","article-title":"Influence of the phosphate backbone on the recognition and hydrolysis of DNA by the EcoRV restriction endonuclease. A study using oligodeoxynucleotide phosphorothioates","volume":"271","author":"Thorogood","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bpj.2010.08.005_bib17","first-page":"623","article-title":"Preparation of DNA-duplexes, containing internucleotide thiophosphate groups in various positions of the recognition site for the EcoRII restriction endonuclease","volume":"29","author":"Subach","year":"2003","journal-title":"Bioorg. Khim."},{"key":"10.1016\/j.bpj.2010.08.005_bib18","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1093\/nar\/23.3.530","article-title":"Analysis of oligo(deoxynucleoside phosphorothioate)s and their diastereomeric composition","volume":"23","author":"Wilk","year":"1995","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bpj.2010.08.005_bib19","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1080\/15257770008035005","article-title":"Influence of\u00a0diastereomeric ratios of deoxyribonucleoside phosphoramidites on\u00a0the synthesis of phosphorothioate oligonucleotides","volume":"19","author":"Cheruvallath","year":"2000","journal-title":"Nucleosides Nucleotides Nucleic Acids"},{"key":"10.1016\/j.bpj.2010.08.005_bib20","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1021\/j150660a018","article-title":"Analysis of protein-lipid interactions based on model simulations of electron spin resonance spectra","volume":"88","author":"Meirovitch","year":"1984","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bpj.2010.08.005_bib21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0006-3495(87)83217-4","article-title":"Brownian dynamics simulation of restricted rotational diffusion","volume":"52","author":"Mart\u00ednez","year":"1987","journal-title":"Biophys. J."},{"key":"10.1016\/j.bpj.2010.08.005_bib22","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1021\/bi002645h","article-title":"Molecular motion of spin labeled side chains in \u03b1-helices: analysis by variation of side chain structure","volume":"40","author":"Columbus","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2010.08.005_bib23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1006\/jmra.1996.0113","article-title":"Nonlinear-least-squares analysis of slow-motion EPR spectra in one and two dimensions using\u00a0a modified Levenberg-Marquardt algorithm","volume":"120","author":"Budil","year":"1996","journal-title":"J. Magn. Reson. A."},{"key":"10.1016\/j.bpj.2010.08.005_bib24","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.1021\/jp910606h","article-title":"Multifrequency electron spin resonance study of the dynamics of spin labeled T4 lysozyme","volume":"114","author":"Zhang","year":"2010","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bpj.2010.08.005_bib25","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1073\/pnas.95.4.1460","article-title":"A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics","volume":"95","author":"SantaLucia","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2010.08.005_bib26","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1021\/jp983699s","article-title":"Thermodynamic parameters for stacking and hydrogen bonding of nucleic acid bases in aqueous solution: ab initio\/Langevin dipoles study","volume":"103","author":"Florian","year":"1999","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bpj.2010.08.005_bib27","doi-asserted-by":"crossref","first-page":"5509","DOI":"10.1021\/ja00040a004","article-title":"Site-specific labeling of DNA sequences containing phosphorothioate diesters","volume":"114","author":"Fidanza","year":"1992","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bpj.2010.08.005_bib28","doi-asserted-by":"crossref","first-page":"6641","DOI":"10.1016\/j.tetlet.2005.07.158","article-title":"New approach to the synthesis of oligodeoxyribonucleotides modified with phosphorothioates of predetermined sense of P-chirality","volume":"46","author":"Nawrot","year":"2005","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.bpj.2010.08.005_bib29","doi-asserted-by":"crossref","first-page":"16031","DOI":"10.1021\/ja805780u","article-title":"Solid-phase synthesis of stereoregular oligodeoxyribonucleoside phosphorothioates using bicyclic oxazaphospholidine derivatives as monomer units","volume":"130","author":"Oka","year":"2008","journal-title":"J. Am. Chem. Soc."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349510009719?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349510009719?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:11:47Z","timestamp":1757779907000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349510009719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":29,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["S0006349510009719"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2010.08.005","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Nucleotide-Independent Nitroxide Probe Reports on Site-Specific Stereomeric Environment in DNA","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2010.08.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}