{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:12:11Z","timestamp":1791317531967,"version":"4.3.1"},"reference-count":42,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":684,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2011,9]]},"DOI":"10.1016\/j.bpj.2011.08.008","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T14:15:14Z","timestamp":1316528114000},"page":"1539-1545","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":202,"title":["In\u00a0Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy"],"prefix":"10.1016","volume":"101","author":[{"given":"Giuliano","family":"Scarcelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilhan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok\u00a0Hyun","family":"Yun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2011.08.008_bib1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1038\/nphoton.2008.187","article-title":"The eye's aplanatic answer","volume":"2","author":"Artal","year":"2008","journal-title":"Nat. Photonics."},{"key":"10.1016\/j.bpj.2011.08.008_bib2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1126\/science.877544","article-title":"The vertebrate eye lens","volume":"197","author":"Bloemendal","year":"1977","journal-title":"Science"},{"key":"10.1016\/j.bpj.2011.08.008_bib3","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.exer.2010.01.013","article-title":"On the growth and internal structure of the human lens","volume":"90","author":"Augusteyn","year":"2010","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1364\/AO.10.000459","article-title":"Theory of transparency of the eye","volume":"10","author":"Benedek","year":"1971","journal-title":"Appl. Opt."},{"key":"10.1016\/j.bpj.2011.08.008_bib5","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/302415a0","article-title":"Short-range order of crystallin proteins accounts for eye lens transparency","volume":"302","author":"Delaye","year":"1983","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2011.08.008_bib6","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1016\/S0006-3495(96)79477-8","article-title":"Structural basis of eye lens transparency: light scattering by concentrated solutions of bovine \u03b1-crystallin proteins","volume":"71","author":"Xia","year":"1996","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2011.08.008_bib7","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1146\/annurev.bioeng.6.040803.140055","article-title":"Ocular biomechanics and biotransport","volume":"6","author":"Ethier","year":"2004","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.bpj.2011.08.008_bib8","first-page":"956","article-title":"Massive increase in the stiffness of the human lens nucleus with age: the basis for presbyopia?","volume":"10","author":"Heys","year":"2004","journal-title":"Mol. Vis."},{"key":"10.1016\/j.bpj.2011.08.008_bib9","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1159\/000090645","article-title":"Presbyopia: the first stage of nuclear cataract?","volume":"38","author":"McGinty","year":"2006","journal-title":"Ophthalmic Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib10","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1016\/S0042-6989(98)00283-1","article-title":"Biometric, optical and physical changes in the isolated human crystalline lens with age in relation to presbyopia","volume":"39","author":"Glasser","year":"1999","journal-title":"Vision Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib11","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.exer.2004.10.010","article-title":"Dynamic mechanical properties of human lenses","volume":"80","author":"Weeber","year":"2005","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib12","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1136\/bjo.2011.202895","article-title":"Viscoelastic properties of fresh human lenses under 40 years of age: implications for the aetiology of presbyopia","volume":"95","author":"Schachar","year":"2011","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.bpj.2011.08.008_bib13","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.exer.2007.07.012","article-title":"On the relationship between lens stiffness and accommodative amplitude","volume":"85","author":"Weeber","year":"2007","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib14","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.exer.2007.12.009","article-title":"The stiffness of human cataract lenses is a function of both age and the type of cataract","volume":"86","author":"Heys","year":"2008","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib15","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/j.1444-0938.2008.00260.x","article-title":"Restoration of accommodation: surgical options for correction of presbyopia","volume":"91","author":"Glasser","year":"2008","journal-title":"Clin. Exp. Optom."},{"key":"10.1016\/j.bpj.2011.08.008_bib16","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.exer.2010.10.010","article-title":"An improved spinning lens test to determine the stiffness of the human lens","volume":"92","author":"Burd","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib17","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1113\/jphysiol.1971.sp009315","article-title":"The elastic constants of the human lens","volume":"212","author":"Fisher","year":"1971","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.bpj.2011.08.008_bib18","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0042-6989(97)00102-8","article-title":"Presbyopia and the optical changes in the human crystalline lens with age","volume":"38","author":"Glasser","year":"1998","journal-title":"Vision Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib19","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1007\/s00417-007-0537-1","article-title":"Stiffness gradient in the crystalline lens","volume":"245","author":"Weeber","year":"2007","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.bpj.2011.08.008_bib20","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.exer.2010.06.003","article-title":"Mouse lens stiffness measurements","volume":"91","author":"Baradia","year":"2010","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib21","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.exer.2006.10.007","article-title":"Mapping age-related elasticity changes in porcine lenses using bubble-based acoustic radiation force","volume":"84","author":"Erpelding","year":"2007","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib22","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1006\/exer.1994.1147","article-title":"Relation between local acoustic parameters and protein distribution in human and porcine eye lenses","volume":"59","author":"De Korte","year":"1994","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib23","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/nphoton.2007.250","article-title":"Confocal Brillouin microscopy for three-dimensional mechanical imaging","volume":"2","author":"Scarcelli","year":"2008","journal-title":"Nat. Photonics."},{"key":"10.1016\/j.bpj.2011.08.008_bib24","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/284489a0","article-title":"Brillouin scattering, density and elastic properties of the lens and cornea of the eye","volume":"284","author":"Vaughan","year":"1980","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2011.08.008_bib25","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.1109\/TBME.2010.2052393","article-title":"Light-scattering study of the normal human eye lens: elastic properties and age dependence","volume":"57","author":"Bailey","year":"2010","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"10.1016\/j.bpj.2011.08.008_bib26","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.1364\/OL.33.002979","article-title":"Cross-axis cascading of spectral dispersion","volume":"33","author":"Scarcelli","year":"2008","journal-title":"Opt. Lett."},{"key":"10.1016\/j.bpj.2011.08.008_bib27","doi-asserted-by":"crossref","first-page":"10913","DOI":"10.1364\/OE.19.010913","article-title":"Multistage VIPA etalons for high-extinction parallel Brillouin spectroscopy","volume":"19","author":"Scarcelli","year":"2011","journal-title":"Opt. Express"},{"key":"10.1016\/j.bpj.2011.08.008_bib28","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/sj.eye.6701525","article-title":"Refractive index distribution in the porcine eye lens for 532\u00a0nm and 633\u00a0nm light","volume":"19","author":"Pierscionek","year":"2005","journal-title":"Eye (Lond.)"},{"key":"10.1016\/j.bpj.2011.08.008_bib29","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1016\/0042-6989(94)90023-X","article-title":"Refractive index of decapsulated bovine lens surfaces measured with a reflectometric sensor","volume":"34","author":"Pierscionek","year":"1994","journal-title":"Vision Res."},{"key":"10.1016\/j.bpj.2011.08.008_bib30","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/eye.1995.194","article-title":"The refractive index along the optic axis of the bovine lens","volume":"9","author":"Pierscionek","year":"1995","journal-title":"Eye (Lond.)"},{"key":"10.1016\/j.bpj.2011.08.008_bib31","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1136\/bjo.2006.105965","article-title":"Viscoelastic shear properties of the fresh porcine lens","volume":"91","author":"Schachar","year":"2007","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.bpj.2011.08.008_bib32","series-title":"Cytoskeletal Mechanics","year":"2006"},{"key":"10.1016\/j.bpj.2011.08.008_bib33","doi-asserted-by":"crossref","first-page":"148102","DOI":"10.1103\/PhysRevLett.87.148102","article-title":"Scaling the microrheology of living cells","volume":"87","author":"Fabry","year":"2001","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2011.08.008_bib34","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1103\/PhysRevLett.78.2020","article-title":"Rheology of soft glassy materials","volume":"78","author":"Sollich","year":"1997","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2011.08.008_bib35","doi-asserted-by":"crossref","first-page":"R1241","DOI":"10.1103\/PhysRevE.58.R1241","article-title":"Dynamic shear modulus of a semiflexible polymer network","volume":"58","author":"Gittes","year":"1998","journal-title":"Phys. Rev. E Stat. Phys. Plasmas Fluids Relat. Interdiscip. Topics"},{"key":"10.1016\/j.bpj.2011.08.008_bib36","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s10439-009-9661-x","article-title":"Biomechanics: cell research and applications for the next decade","volume":"37","author":"Discher","year":"2009","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2011.08.008_bib37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev.bioeng.5.040202.121623","article-title":"Selected methods for imaging elastic properties of biological tissues","volume":"5","author":"Greenleaf","year":"2003","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.bpj.2011.08.008_bib38","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/nmat1685","article-title":"Fast and slow dynamics of the cytoskeleton","volume":"5","author":"Deng","year":"2006","journal-title":"Nat. Mater."},{"key":"10.1016\/j.bpj.2011.08.008_bib39","series-title":"Physical Properties of Tissue","author":"Duck","year":"1990"},{"key":"10.1016\/j.bpj.2011.08.008_bib40","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1121\/1.3268508","article-title":"A unifying fractional wave equation for compressional and shear waves","volume":"127","author":"Holm","year":"2010","journal-title":"J.\u00a0Acoust. Soc. Am."},{"key":"10.1016\/j.bpj.2011.08.008_bib41","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1038\/nature01895","article-title":"Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens","volume":"424","author":"Nishimoto","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2011.08.008_bib42","unstructured":"Sistla, P. A., M. A. Reilly, \u2026, N. Ravi. 2009. The effect of R120G mutation in \u03b1B-Crystallin on the mechanical properties of mouse lenses. Proc. ARVO Reducing Disparit. Eye Dis. Treat., Ft. Lauderdale, FL. Abstract 2107."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349511009507?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349511009507?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:15:49Z","timestamp":1757780149000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349511009507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,9]]}},"alternative-id":["S0006349511009507"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2011.08.008","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2011,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2011.08.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}