{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:57:32Z","timestamp":1758405452938},"reference-count":33,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,8,5]],"date-time":"2015-08-05T00:00:00Z","timestamp":1438732800000},"content-version":"vor","delay-in-days":369,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["biophysj.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1016\/j.bpj.2014.06.030","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T11:51:52Z","timestamp":1407239512000},"page":"613-620","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Blocking Dynamics of the SMR Transporter EmrE Impairs Efflux Activity"],"prefix":"10.1016","volume":"107","author":[{"given":"Supratik","family":"Dutta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma\u00a0A.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine\u00a0A.","family":"Henzler-Wildman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2014.06.030_bib1","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1038\/211969a0","article-title":"Simple allosteric model for membrane pumps","volume":"211","author":"Jardetzky","year":"1966","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2014.06.030_bib2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nature12233","article-title":"Structural basis for alternating access of a eukaryotic calcium\/proton exchanger","volume":"499","author":"Waight","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2014.06.030_bib3","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1515\/hsz-2012-0336","article-title":"Mechanistic aspects of sodium-binding sites in LeuT-like fold symporters","volume":"394","author":"Perez","year":"2013","journal-title":"Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib4","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.bbabio.2010.10.014","article-title":"The structural basis of secondary active transport mechanisms","volume":"1807","author":"Forrest","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.06.030_bib5","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.sbi.2008.09.007","article-title":"Structural insights into ABC transporter mechanism","volume":"18","author":"Oldham","year":"2008","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.bpj.2014.06.030_bib6","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nature12811","article-title":"Structural basis of the alternating-access mechanism in a bile acid transporter","volume":"505","author":"Zhou","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2014.06.030_bib7","doi-asserted-by":"crossref","first-page":"44036","DOI":"10.1074\/jbc.M112.414433","article-title":"Cysteine cross-linking defines the extracellular gate for the Leishmania donovani nucleoside transporter 1.1 (LdNT1.1)","volume":"287","author":"Vald\u00e9s","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib8","doi-asserted-by":"crossref","first-page":"33807","DOI":"10.1074\/jbc.M109.071977","article-title":"Ligand effects on cross-linking support a conformational mechanism for serotonin transport","volume":"284","author":"Tao","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib9","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/978-1-60761-652-8_24","article-title":"Site-directed disulfide cross-linking\u00a0to probe conformational changes of a transporter during its functional cycle: Escherichia coli AcrB multidrug exporter as an example","volume":"634","author":"Takatsuka","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bpj.2014.06.030_bib10","doi-asserted-by":"crossref","first-page":"20659","DOI":"10.1073\/pnas.0708639104","article-title":"CLC Cl \/H+ transporters constrained by covalent cross-linking","volume":"104","author":"Nguitragool","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.06.030_bib11","doi-asserted-by":"crossref","first-page":"15141","DOI":"10.1073\/pnas.1112216108","article-title":"Large collective motions regulate the functional properties of glutamate transporter trimers","volume":"108","author":"Jiang","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.06.030_bib12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/nature10703","article-title":"Antiparallel EmrE exports drugs by exchanging between asymmetric structures","volume":"481","author":"Morrison","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2014.06.030_bib13","doi-asserted-by":"crossref","first-page":"6825","DOI":"10.1074\/jbc.M113.535328","article-title":"Transported substrate determines exchange rate in the multidrug resistance transporter EmrE","volume":"289","author":"Morrison","year":"2014","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib14","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1093\/jb\/mvm169","article-title":"Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE","volume":"142","author":"Nara","year":"2007","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.bpj.2014.06.030_bib15","doi-asserted-by":"crossref","first-page":"15234","DOI":"10.1074\/jbc.M110.108746","article-title":"Topologically random insertion of EmrE supports a pathway for evolution of inverted repeats in ion-coupled transporters","volume":"285","author":"Nasie","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib16","doi-asserted-by":"crossref","first-page":"3036","DOI":"10.1016\/j.bbamem.2007.09.012","article-title":"MAS solid-state NMR studies on the multidrug transporter EmrE","volume":"1768","author":"Agarwal","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.06.030_bib17","doi-asserted-by":"crossref","first-page":"15754","DOI":"10.1021\/ja402605s","article-title":"Detecting substrates bound to the secondary multidrug efflux pump EmrE by DNP-enhanced solid-state NMR","volume":"135","author":"Ong","year":"2013","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.bpj.2014.06.030_bib18","doi-asserted-by":"crossref","first-page":"10321","DOI":"10.1002\/anie.201303091","article-title":"Ligand-induced conformational changes of the multidrug resistance transporter EmrE probed by oriented solid-state NMR spectroscopy","volume":"52","author":"Gayen","year":"2013","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bpj.2014.06.030_bib19","doi-asserted-by":"crossref","first-page":"6175","DOI":"10.1093\/emboj\/cdg611","article-title":"Three-dimensional structure of the bacterial multidrug transporter EmrE shows it is an asymmetric homodimer","volume":"22","author":"Ubarretxena-Belandia","year":"2003","journal-title":"EMBO\u00a0J."},{"key":"10.1016\/j.bpj.2014.06.030_bib20","doi-asserted-by":"crossref","first-page":"18999","DOI":"10.1073\/pnas.0709387104","article-title":"X-ray structure of EmrE supports dual topology model","volume":"104","author":"Chen","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.06.030_bib21","doi-asserted-by":"crossref","first-page":"4642","DOI":"10.1016\/j.jmb.2013.07.039","article-title":"In\u00a0vivo trp scanning of the small multidrug resistance protein EmrE confirms 3D structure models\u2019","volume":"425","author":"Lloris-Garcer\u00e1","year":"2013","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bpj.2014.06.030_bib22","doi-asserted-by":"crossref","first-page":"26052","DOI":"10.1074\/jbc.M112.357590","article-title":"Antiparallel dimers of the small multidrug resistance protein EmrE are more stable than parallel dimers","volume":"287","author":"Lloris-Garcer\u00e1","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jmb.2006.08.072","article-title":"Quasi-symmetry in the cryo-EM structure of EmrE provides the key to modeling its transmembrane domain","volume":"364","author":"Fleishman","year":"2006","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bpj.2014.06.030_bib24","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/j.bbamem.2011.12.020","article-title":"Reconstitution of integral membrane proteins into isotropic bicelles with improved sample stability and expanded lipid composition profile","volume":"1818","author":"Morrison","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.06.030_bib25","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s10858-009-9385-0","article-title":"TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins","volume":"45","author":"Li","year":"2009","journal-title":"J.\u00a0Biomol. NMR"},{"key":"10.1016\/j.bpj.2014.06.030_bib26","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"Delaglio","year":"1995","journal-title":"J.\u00a0Biomol. NMR"},{"key":"10.1016\/j.bpj.2014.06.030_bib27","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/BF00404272","article-title":"NMRView: a computer program for the visualization and analysis of NMR data","volume":"4","author":"Johnson","year":"1994","journal-title":"J.\u00a0Biomol. NMR"},{"key":"10.1016\/j.bpj.2014.06.030_bib28","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1074\/jbc.270.12.6856","article-title":"EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents","volume":"270","author":"Yerushalmi","year":"1995","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib29","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1021\/bi00296a027","article-title":"Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells","volume":"23","author":"Lambert","year":"1984","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2014.06.030_bib30","doi-asserted-by":"crossref","first-page":"26710","DOI":"10.1074\/jbc.M110.132621","article-title":"Structure, dynamics, and substrate-induced conformational changes of the multidrug transporter EmrE in liposomes","volume":"285","author":"Amadi","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib31","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1074\/jbc.M707899200","article-title":"The key residue for substrate transport (Glu-14) in the EmrE dimer is asymmetric","volume":"283","author":"Lehner","year":"2008","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.06.030_bib32","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1016\/j.jmb.2008.01.056","article-title":"Electron crystallography reveals plasticity within the drug binding site of the small multidrug transporter EmrE","volume":"377","author":"Korkhov","year":"2008","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bpj.2014.06.030_bib33","doi-asserted-by":"crossref","first-page":"8072","DOI":"10.1021\/ja503145x","article-title":"Intrinsic Conformational Plasticity of Native EmrE Provides a Pathway for Multidrug Resistance","volume":"136","author":"Cho","year":"2014","journal-title":"J. Am. Chem. Soc."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349514006730?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349514006730?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:52:15Z","timestamp":1538355135000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349514006730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["S0006349514006730"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2014.06.030","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Blocking Dynamics of the SMR Transporter EmrE Impairs Efflux Activity","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2014.06.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}