{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:24:18Z","timestamp":1785911058779,"version":"3.56.0"},"reference-count":55,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,11,4]],"date-time":"2015-11-04T00:00:00Z","timestamp":1446595200000},"content-version":"vor","delay-in-days":368,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["biophysj.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2014,11]]},"DOI":"10.1016\/j.bpj.2014.09.034","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T16:48:28Z","timestamp":1415119708000},"page":"2082-2090","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":57,"title":["Physical Properties of Escherichia coli Spheroplast Membranes"],"prefix":"10.1016","volume":"107","author":[{"given":"Yen","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Lin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey\u00a0W.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2014.09.034_bib1","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1146\/annurev.bb.23.060194.004035","article-title":"Mechanical properties of the red cell membrane in relation to molecular structure and genetic defects","volume":"23","author":"Mohandas","year":"1994","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"10.1016\/j.bpj.2014.09.034_bib2","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1016\/S0006-3495(92)81970-7","article-title":"A sensitive measure of surface stress in the resting neutrophil","volume":"61","author":"Needham","year":"1992","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib3","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1016\/S0006-3495(94)81012-4","article-title":"Passive mechanical behavior of human neutrophils: effect of cytochalasin B","volume":"66","author":"Tsai","year":"1994","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0006-3495(89)82660-8","article-title":"Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration","volume":"56","author":"Evans","year":"1989","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0021-9290(99)00175-X","article-title":"Micropipette aspiration of living cells","volume":"33","author":"Hochmuth","year":"2000","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2014.09.034_bib6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0962-8924(96)80993-7","article-title":"Modulation of membrane dynamics and cell motility by membrane tension","volume":"6","author":"Sheetz","year":"1996","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.bpj.2014.09.034_bib7","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1146\/annurev.biophys.35.040405.102017","article-title":"Continuous membrane-cytoskeleton adhesion requires continuous accommodation to lipid and cytoskeleton dynamics","volume":"35","author":"Sheetz","year":"2006","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"10.1016\/j.bpj.2014.09.034_bib8","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/35073095","article-title":"Cell control by membrane-cytoskeleton adhesion","volume":"2","author":"Sheetz","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bpj.2014.09.034_bib9","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.tcb.2012.07.005","article-title":"Mechanical feedback between membrane tension and dynamics","volume":"22","author":"Gauthier","year":"2012","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.bpj.2014.09.034_bib10","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1016\/S0006-3495(99)77168-7","article-title":"Membrane tether formation from blebbing cells","volume":"77","author":"Dai","year":"1999","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib11","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0021-9290(98)00166-3","article-title":"Alterations in the Young\u2019s modulus and volumetric properties of chondrocytes isolated from normal and osteoarthritic human cartilage","volume":"32","author":"Jones","year":"1999","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2014.09.034_bib12","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1073\/pnas.84.8.2297","article-title":"Pressure-sensitive ion channel in Escherichia coli","volume":"84","author":"Martinac","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.09.034_bib13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0005-2736(85)90200-7","article-title":"Fusion of bacterial spheroplasts by electric fields","volume":"819","author":"Ruthe","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.09.034_bib14","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1073\/pnas.1015757108","article-title":"Cardiolipin microdomains localize to negatively curved regions of Escherichia coli membranes","volume":"108","author":"Renner","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.09.034_bib15","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1128\/AAC.02005-12","article-title":"The target of daptomycin is absent from Escherichia coli and other gram-negative pathogens","volume":"57","author":"Randall","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.bpj.2014.09.034_bib16","series-title":"The Physiology and Biochemistry of Prokaryoties","author":"White","year":"2012"},{"key":"10.1016\/j.bpj.2014.09.034_bib17","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1128\/MMBR.63.1.230-262.1999","article-title":"Osmosensing by bacteria: signals and membrane-based sensors","volume":"63","author":"Wood","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.bpj.2014.09.034_bib18","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1126\/science.282.5397.2220","article-title":"Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel","volume":"282","author":"Chang","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.bpj.2014.09.034_bib19","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.bbamem.2004.06.009","article-title":"Lipids do influence protein function\u2014the hydrophobic matching hypothesis revisited","volume":"1666","author":"Jensen","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.09.034_bib20","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1038\/ncb2480","article-title":"Plasma membrane stress induces relocalization of Slm proteins and activation of TORC2 to promote sphingolipid synthesis","volume":"14","author":"Berchtold","year":"2012","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bpj.2014.09.034_bib21","doi-asserted-by":"crossref","first-page":"E77","DOI":"10.1073\/pnas.1101130108","article-title":"Real-time attack on single Escherichia coli cells by the human antimicrobial peptide LL-37","volume":"108","author":"Sochacki","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.09.034_bib22","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.bbamem.2013.02.011","article-title":"Real-time attack of LL-37 on single Bacillus subtilis cells","volume":"1828","author":"Barns","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.09.034_bib23","doi-asserted-by":"crossref","first-page":"6584","DOI":"10.1021\/bi400785j","article-title":"Localized permeabilization of E. coli membranes by the antimicrobial peptide Cecropin A","volume":"52","author":"Rangarajan","year":"2013","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2014.09.034_bib24","doi-asserted-by":"crossref","first-page":"4494","DOI":"10.1128\/JB.00011-12","article-title":"Daptomycin-mediated reorganization of membrane architecture causes mislocalization of essential cell division proteins","volume":"194","author":"Pogliano","year":"2012","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.bpj.2014.09.034_bib25","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1016\/j.bpj.2009.01.030","article-title":"Free energies of molecular bound states in lipid bilayers: lethal concentrations of antimicrobial peptides","volume":"96","author":"Huang","year":"2009","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib26","first-page":"272","article-title":"Osmoadaptation in bacteria","volume":"37","author":"Galinski","year":"1995","journal-title":"Adv. Microb. Physiol."},{"key":"10.1016\/j.bpj.2014.09.034_bib27","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/BF00174204","article-title":"Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying","volume":"37","author":"Lippert","year":"1992","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.bpj.2014.09.034_bib28","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1128\/jb.170.6.2457-2461.1988","article-title":"Osmotic regulation of biosynthesis of membrane-derived oligosaccharides in Escherichia coli","volume":"170","author":"Kennedy","year":"1988","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.bpj.2014.09.034_bib29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0968-0004(98)01196-7","article-title":"Responses of E. coli to osmotic stress: large changes in amounts of cytoplasmic solutes and water","volume":"23","author":"Record","year":"1998","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.bpj.2014.09.034_bib30","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/S0006-3495(00)76726-9","article-title":"Biophysical characterization of changes in amounts and activity of Escherichia coli cell and compartment water and turgor pressure in response to osmotic stress","volume":"78","author":"Cayley","year":"2000","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib31","doi-asserted-by":"crossref","first-page":"7850","DOI":"10.1016\/S0021-9258(17)41044-1","article-title":"Periplasmic space in Salmonella typhimurium and Escherichia coli","volume":"252","author":"Stock","year":"1977","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.09.034_bib32","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1016\/S0021-9258(19)57283-0","article-title":"Porin channels in intact cells of Escherichia coli are not affected by Donnan potentials across the outer membrane","volume":"263","author":"Sen","year":"1988","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2014.09.034_bib33","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S0006-3495(81)84817-5","article-title":"Thermoelasticity of large lecithin bilayer vesicles","volume":"35","author":"Kwok","year":"1981","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib34","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.bpj.2008.11.007","article-title":"Interaction of tea catechin (-)-epigallocatechin gallate with lipid bilayers","volume":"96","author":"Sun","year":"2009","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib35","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.bpj.2011.01.013","article-title":"Adhesion and merging of lipid bilayers: a method for measuring the free energy of adhesion and hemifusion","volume":"100","author":"Sun","year":"2011","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib36","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1128\/JB.138.2.567-574.1979","article-title":"Biochemical and topographical studies on Escherichia coli cell surface. IV. Giant spheroplast formation from a filamentous cell","volume":"138","author":"Onitsuka","year":"1979","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.bpj.2014.09.034_bib37","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/JB.155.1.228-237.1983","article-title":"Coordination of flagella on filamentous cells of Escherichia coli","volume":"155","author":"Ishihara","year":"1983","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.bpj.2014.09.034_bib38","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0005-2736(95)00126-N","article-title":"Generation of giant protoplasts of Escherichia coli and an inner-membrane anion selective conductance","volume":"1238","author":"Kubalski","year":"1995","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.09.034_bib39","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0005-2736(90)90214-9","article-title":"Ion channel activities in the Escherichia coli outer membrane","volume":"1024","author":"Buechner","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2014.09.034_bib40","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/S0006-3495(00)76295-3","article-title":"Effect of chain length and unsaturation on elasticity of lipid bilayers","volume":"79","author":"Rawicz","year":"2000","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2014.09.034_bib41","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1073\/pnas.0710625105","article-title":"Mechanism and kinetics of pore formation in membranes by water-soluble amphipathic peptides","volume":"105","author":"Lee","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.09.034_bib42","doi-asserted-by":"crossref","first-page":"14243","DOI":"10.1073\/pnas.1307010110","article-title":"Process of inducing pores in membranes by melittin","volume":"110","author":"Lee","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2014.09.034_bib43","series-title":"Theory of Elasticity","first-page":"101","author":"Landau","year":"1970"},{"key":"10.1016\/j.bpj.2014.09.034_bib44","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1126\/science.347575","article-title":"Models for the specific adhesion of cells to cells","volume":"200","author":"Bell","year":"1978","journal-title":"Science"},{"key":"10.1016\/j.bpj.2014.09.034_bib45","doi-asserted-by":"crossref","first-page":"118304","DOI":"10.1103\/PhysRevLett.109.118304","article-title":"Universal relaxation governs the nonequilibrium elasticity of biomolecules","volume":"109","author":"Kappel","year":"2012","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2014.09.034_bib46","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1126\/science.7939660","article-title":"Intermolecular forces and energies between ligands and receptors","volume":"266","author":"Moy","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.bpj.2014.09.034_bib47","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1126\/science.276.5315.1112","article-title":"Folding-unfolding transitions in single titin molecules characterized with laser tweezers","volume":"276","author":"Kellermayer","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.bpj.2014.09.034_bib48","doi-asserted-by":"crossref","first-page":"789652","DOI":"10.1155\/2012\/789652","article-title":"Thermal adaptation of the archaeal and bacterial lipid membranes","volume":"2012","author":"Koga","year":"2012","journal-title":"Archaea"},{"key":"10.1016\/j.bpj.2014.09.034_bib49","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1128\/AEM.63.6.2421-2431.1997","article-title":"Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain","volume":"63","author":"Roth","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.bpj.2014.09.034_bib50","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1574-6968.1997.tb10396.x","article-title":"Effects of carbonylcyanide-m-chlorophenylhydrazone (CCCP) and acetate on Escherichia coli O157:H7 and K-12: uncoupling versus anion accumulation","volume":"151","author":"Diez-Gonzalez","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.bpj.2014.09.034_bib51","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1128\/IAI.66.6.2434-2440.1998","article-title":"Structure-function relationship of antibacterial synthetic peptides homologous to a helical surface region on human lactoferrin against Escherichia coli serotype O111","volume":"66","author":"Chapple","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bpj.2014.09.034_bib52","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/jac\/43.1.61","article-title":"Quinolone accumulation by Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli","volume":"43","author":"Piddock","year":"1999","journal-title":"J.\u00a0Antimicrob. Chemother."},{"key":"10.1016\/j.bpj.2014.09.034_bib53","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/nphoton.2011.100","article-title":"Optical tweezers study life under tension","volume":"5","author":"Fazal","year":"2011","journal-title":"Nat. Photonics"},{"key":"10.1016\/j.bpj.2014.09.034_bib54","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1085\/jgp.200910371","article-title":"Adaptive behavior of bacterial mechanosensitive channels is coupled to membrane mechanics","volume":"135","author":"Belyy","year":"2010","journal-title":"J.\u00a0Gen. Physiol."},{"key":"10.1016\/j.bpj.2014.09.034_bib55","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1515\/znc-1973-11-1209","article-title":"Elastic properties of lipid bilayers: theory and possible experiments","volume":"28","author":"Helfrich","year":"1973","journal-title":"Z.\u00a0Naturforsch. C"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000634951401008X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000634951401008X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T05:27:56Z","timestamp":1650518876000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000634951401008X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":55,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["S000634951401008X"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2014.09.034","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2014,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Physical Properties of Escherichia coli Spheroplast Membranes","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2014.09.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}