{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:43:39Z","timestamp":1790660619582,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T00:00:00Z","timestamp":1584576000000},"content-version":"vor","delay-in-days":384,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100005492","name":"Stanford University","doi-asserted-by":"publisher","award":["W911NF07200271"],"award-info":[{"award-number":["W911NF07200271"]}],"id":[{"id":"10.13039\/100005492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2019,3]]},"DOI":"10.1016\/j.bpj.2019.01.040","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T15:01:00Z","timestamp":1549638060000},"page":"1136-1151","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["In\u00a0Vitro Measurement and Modeling of Platelet Adhesion on VWF-Coated Surfaces in Channel Flow"],"prefix":"10.1016","volume":"116","author":[{"given":"Qin M.","family":"Qi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eimear","family":"Dunne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Oglesby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingmar","family":"Schoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio J.","family":"Ricco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dermot","family":"Kenny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.G.","family":"Shaqfeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2019.01.040_bib1","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1161\/ATVBAHA.110.207522","article-title":"Signaling during platelet adhesion and activation","volume":"30","author":"Li","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol"},{"key":"10.1016\/j.bpj.2019.01.040_bib2","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1242\/jcs.01325","article-title":"Platelet adhesion signalling and the regulation of thrombus formation","volume":"117","author":"Gibbins","year":"2004","journal-title":"J.\u00a0Cell Sci"},{"key":"10.1016\/j.bpj.2019.01.040_bib3","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1172\/JCI113871","article-title":"Fibrinogen-independent platelet adhesion and thrombus formation on subendothelium mediated by glycoprotein IIb-IIIa complex at high shear rate","volume":"83","author":"Weiss","year":"1989","journal-title":"J.\u00a0Clin. Invest"},{"key":"10.1016\/j.bpj.2019.01.040_bib4","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0092-8674(00)80983-6","article-title":"Initiation of platelet adhesion by arrest onto fibrinogen or translocation on von Willebrand factor","volume":"84","author":"Savage","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.bpj.2019.01.040_bib5","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1365-2141.1980.tb01190.x","article-title":"Shear rate dependent inhibition of platelet adhesion and aggregation on collagenous surfaces by antibodies to human factor VIII\/von Willebrand factor","volume":"44","author":"Baumgartner","year":"1980","journal-title":"Br. J. Haematol"},{"key":"10.1016\/j.bpj.2019.01.040_bib6","doi-asserted-by":"crossref","first-page":"16900","DOI":"10.1073\/pnas.0608207103","article-title":"The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis","volume":"103","author":"Bergmeier","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2019.01.040_bib7","doi-asserted-by":"crossref","first-page":"4648","DOI":"10.1073\/pnas.1501689112","article-title":"Force-induced on-rate switching and modulation by mutations in gain-of-function von Willebrand diseases","volume":"112","author":"Kim","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2019.01.040_bib8","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1038\/nature09295","article-title":"A mechanically stabilized receptor-ligand flex-bond important in the vasculature","volume":"466","author":"Kim","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2019.01.040_bib9","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/s41467-017-00230-2","article-title":"Flow-induced elongation of von Willebrand factor precedes tension-dependent activation","volume":"8","author":"Fu","year":"2017","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2019.01.040_bib10","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1146\/annurev.bioeng.1.1.299","article-title":"Fluid mechanics of vascular systems, diseases, and thrombosis","volume":"1","author":"Wootton","year":"1999","journal-title":"Annu. Rev. Biomed. Eng"},{"key":"10.1016\/j.bpj.2019.01.040_bib11","doi-asserted-by":"crossref","first-page":"011902","DOI":"10.1063\/1.3677935","article-title":"Shear-induced particle migration and margination in a cellular suspension","volume":"24","author":"Zhao","year":"2012","journal-title":"Phys. Fluids"},{"key":"10.1016\/j.bpj.2019.01.040_bib12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.bpj.2016.06.024","article-title":"The effect of hematocrit on platelet adhesion: experiments and simulations","volume":"111","author":"Spann","year":"2016","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2019.01.040_bib13","doi-asserted-by":"crossref","first-page":"093102","DOI":"10.1103\/PhysRevFluids.2.093102","article-title":"Theory to predict particle migration and margination in the pressure-driven channel flow of blood","volume":"2","author":"Qi","year":"2017","journal-title":"Phys. Rev. Fluids"},{"key":"10.1016\/j.bpj.2019.01.040_bib14","doi-asserted-by":"crossref","first-page":"034302","DOI":"10.1103\/PhysRevFluids.3.034302","article-title":"Time-dependent particle migration and margination in the pressure-driven channel flow of blood","volume":"3","author":"Qi","year":"2018","journal-title":"Phys. Rev. Fluids"},{"key":"10.1016\/j.bpj.2019.01.040_bib15","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1016\/j.bpj.2015.04.013","article-title":"In\u00a0vitro measurement of particle margination in the microchannel flow: effect of varying hematocrit","volume":"108","author":"Fitzgibbon","year":"2015","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2019.01.040_bib16","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1002\/j.2040-4603.2016.tb00700.x","article-title":"Hemodynamics","volume":"6","author":"Secomb","year":"2016","journal-title":"Compr. Physiol"},{"key":"10.1016\/j.bpj.2019.01.040_bib17","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1046\/j.1537-2995.2001.41080977.x","article-title":"Anemia-induced increase in the bleeding time: implications for treatment of nonsurgical blood loss","volume":"41","author":"Valeri","year":"2001","journal-title":"Transfusion"},{"key":"10.1016\/j.bpj.2019.01.040_bib18","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1182\/blood-2016-10-746479","article-title":"Elevated hematocrit enhances platelet accumulation following vascular injury","volume":"129","author":"Walton","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2019.01.040_bib19","doi-asserted-by":"crossref","first-page":"2556","DOI":"10.1529\/biophysj.107.128520","article-title":"Platelet adhesive dynamics. Part\u00a0II:\u00a0high shear-induced transient aggregation via GPIbalpha-vWF-GPIbalpha bridging","volume":"95","author":"Mody","year":"2008","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2019.01.040_bib20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jcp.2012.08.014","article-title":"Multiscale model of platelet translocation and collision","volume":"244","author":"Wang","year":"2013","journal-title":"J.\u00a0Comput. Phys"},{"key":"10.1016\/j.bpj.2019.01.040_bib21","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1039\/C4SM01450B","article-title":"Examining platelet adhesion via Stokes flow simulations and microfluidic experiments","volume":"11","author":"Fitzgibbon","year":"2015","journal-title":"Soft Matter"},{"key":"10.1016\/j.bpj.2019.01.040_bib22","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1039\/C4SM02686A","article-title":"Microparticle shape effects on margination, near-wall dynamics and adhesion in a three-dimensional simulation of red blood cell suspension","volume":"11","author":"Vahidkhah","year":"2015","journal-title":"Soft Matter"},{"key":"10.1016\/j.bpj.2019.01.040_bib23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jbiomech.2016.11.019","article-title":"A multiscale biomechanical model of platelets: correlating with in-vitro results","volume":"50","author":"Zhang","year":"2017","journal-title":"J.\u00a0Biomech"},{"key":"10.1016\/j.bpj.2019.01.040_bib24","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1016\/j.bpj.2014.04.028","article-title":"Platelet dynamics in three-dimensional simulation of whole blood","volume":"106","author":"Vahidkhah","year":"2014","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2019.01.040_bib25","series-title":"AMS Contemporary Mathematics: Biological Fluid Dynamics: Modeling, Computations, and Applications","first-page":"149","article-title":"Multi-bond models of platelet adhesion and cohesion","author":"Skorczewski","year":"2014"},{"key":"10.1016\/j.bpj.2019.01.040_bib26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3109\/10520299709082249","article-title":"DiOC6(3): a useful dye for staining the endoplasmic reticulum","volume":"72","author":"Sabnis","year":"1997","journal-title":"Biotech. Histochem"},{"key":"10.1016\/j.bpj.2019.01.040_bib27","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.ab.2010.05.030","article-title":"Integrated system investigating shear-mediated platelet interactions with von Willebrand factor using microliters of whole blood","volume":"405","author":"Lincoln","year":"2010","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.bpj.2019.01.040_bib28","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s10544-010-9453-y","article-title":"Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfaces","volume":"12","author":"Kent","year":"2010","journal-title":"Biomed. Microdevices"},{"key":"10.1016\/j.bpj.2019.01.040_bib29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1088\/0967-3334\/23\/2\/315","article-title":"In\u00a0vivo PIV measurement of red blood cell velocity field in microvessels considering mesentery motion","volume":"23","author":"Sugii","year":"2002","journal-title":"Physiol. Meas"},{"key":"10.1016\/j.bpj.2019.01.040_bib30","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1161\/01.RES.43.5.738","article-title":"The distribution of blood rheological parameters in the microvasculature of cat mesentery","volume":"43","author":"Lipowsky","year":"1978","journal-title":"Circ. Res"},{"key":"10.1016\/j.bpj.2019.01.040_bib31","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1080\/09537100020019157","article-title":"Structural determinants within platelet glycoprotein Ibalpha involved in its binding to von Willebrand factor","volume":"11","author":"Cauwenberghs","year":"2000","journal-title":"Platelets"},{"key":"10.1016\/j.bpj.2019.01.040_bib32","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1161\/01.ATV.16.5.611","article-title":"Platelet adhesion to multimeric and dimeric von Willebrand factor and to collagen type III preincubated with von Willebrand factor","volume":"16","author":"Wu","year":"1996","journal-title":"Arterioscler. Thromb. Vasc. Biol"},{"key":"10.1016\/j.bpj.2019.01.040_bib33","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1055\/s-0038-1657620","article-title":"GPIIb\/IIIa antagonists: pathophysiologic and therapeutic insights from studies of c7E3 Fab","volume":"78","author":"Coller","year":"1997","journal-title":"Thromb. Haemost"},{"key":"10.1016\/j.bpj.2019.01.040_bib34","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.3324\/haematol.2013.087221","article-title":"Platelet interaction with von Willebrand factor is enhanced by shear-induced clustering of glycoprotein Ib\u03b1","volume":"98","author":"Gitz","year":"2013","journal-title":"Haematologica"},{"key":"10.1016\/j.bpj.2019.01.040_bib35","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1364\/OE.20.002081","article-title":"Wavelet analysis for single molecule localization microscopy","volume":"20","author":"Izeddin","year":"2012","journal-title":"Opt. Express"},{"key":"10.1016\/j.bpj.2019.01.040_bib36","doi-asserted-by":"crossref","first-page":"14278","DOI":"10.1038\/s41598-017-14346-4","article-title":"Margination and stretching of von Willebrand factor in the blood stream enable adhesion","volume":"7","author":"Rack","year":"2017","journal-title":"Sci. Rep"},{"key":"10.1016\/j.bpj.2019.01.040_bib37","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1182\/blood-2014-05-378638","article-title":"von Willebrand factor, Jedi knight of the bloodstream","volume":"124","author":"Springer","year":"2014","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2019.01.040_bib38","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1182\/blood.V88.8.2939.bloodjournal8882939","article-title":"Shear-dependent changes in the three-dimensional structure of human von Willebrand factor","volume":"88","author":"Siedlecki","year":"1996","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2019.01.040_bib39","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1080\/0953710021000024394","article-title":"Glanzmann\u2019s thrombasthenia: updated","volume":"13","author":"Nair","year":"2002","journal-title":"Platelets"},{"key":"10.1016\/j.bpj.2019.01.040_bib40","doi-asserted-by":"crossref","unstructured":"M\u00fcller, J. P., S. Mielke, \u2026, M. Benoit. Force sensing by the vascular protein von Willebrand factor is tuned by a strong intermonomer interaction. Proc. Natl. Acad. Sci. USA. 113:1208\u20131213.","DOI":"10.1073\/pnas.1516214113"},{"key":"10.1016\/j.bpj.2019.01.040_bib41","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/S0006-3495(98)74074-3","article-title":"Measuring two-dimensional receptor-ligand binding kinetics by micropipette","volume":"75","author":"Chesla","year":"1998","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2019.01.040_bib42","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.thromres.2015.06.019","article-title":"Von Willebrand factor-A1 domain binds platelet glycoprotein Ib\u03b1 in multiple states with distinctive force-dependent dissociation kinetics","volume":"136","author":"Ju","year":"2015","journal-title":"Thromb. Res"},{"key":"10.1016\/j.bpj.2019.01.040_bib43","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0959-440X(03)00039-3","article-title":"Protein-protein unbinding induced by force: single-molecule studies","volume":"13","author":"Weisel","year":"2003","journal-title":"Curr. Opin. Struct. Biol"},{"key":"10.1016\/j.bpj.2019.01.040_bib44","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1146\/annurev.fl.21.010189.001123","article-title":"Biofluid mechanics","volume":"21","author":"Skalak","year":"1989","journal-title":"Annu. Rev. Fluid Mech"},{"key":"10.1016\/j.bpj.2019.01.040_bib45","doi-asserted-by":"crossref","first-page":"113302","DOI":"10.1063\/1.2126937","article-title":"Three-dimensional simulations of a platelet-shaped spheroid near a wall in shear flow","volume":"17","author":"Mody","year":"2005","journal-title":"Phys. Fluids"},{"key":"10.1016\/j.bpj.2019.01.040_bib46","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1182\/blood.V99.1.159","article-title":"Shear-dependent tether formation during platelet translocation on von Willebrand factor","volume":"99","author":"Dopheide","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2019.01.040_bib47","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/0002-9343(74)90170-3","article-title":"Decreased adhesion of giant (Bernard-Soulier) platelets to subendothelium. Further implications on the role of the von Willebrand factor in hemostasis","volume":"57","author":"Weiss","year":"1974","journal-title":"Am. J. Med"},{"key":"10.1016\/j.bpj.2019.01.040_bib48","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1182\/blood-2005-02-0618","article-title":"Mechanism of platelet adhesion to von Willebrand factor and microparticle formation under high shear stress","volume":"107","author":"Reininger","year":"2006","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2019.01.040_bib49","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1161\/01.ATV.20.3.607","article-title":"Platelet glycoprotein IIb\/IIIa receptors and Glanzmann\u2019s thrombasthenia","volume":"20","author":"French","year":"2000","journal-title":"Arterioscler. Thromb. Vasc. Biol"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349519301110?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349519301110?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:34:33Z","timestamp":1762101273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349519301110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["S0006349519301110"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2019.01.040","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2019,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0Vitro Measurement and Modeling of Platelet Adhesion on VWF-Coated Surfaces in Channel Flow","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2019.01.040","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Biophysical Society.","name":"copyright","label":"Copyright"}]}}