{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:40:38Z","timestamp":1772120438837,"version":"3.50.1"},"reference-count":21,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.bpj.2019.08.024","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T19:34:57Z","timestamp":1567020897000},"page":"1258-1268","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Microwell Cell Capture Device Reveals Variable Response to Dobutamine in Isolated Cardiomyocytes"],"prefix":"10.1016","volume":"117","author":[{"given":"J. Alexander","family":"Clark","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan D.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart G.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2019.08.024_bib1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1113\/JP276718","article-title":"Diverse relaxation rates exist among rat cardiomyocytes isolated from a single myocardial region","volume":"597","author":"Clark","year":"2019","journal-title":"J.\u00a0Physiol"},{"key":"10.1016\/j.bpj.2019.08.024_bib2","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/phar.1939","article-title":"Effect of inpatient dobutamine versus milrinone on out-of-hospital mortality in patients with acute decompensated heart failure","volume":"37","author":"King","year":"2017","journal-title":"Pharmacotherapy"},{"key":"10.1016\/j.bpj.2019.08.024_bib3","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1126\/science.1200113","article-title":"Cardiac myosin activation: a potential therapeutic approach for systolic heart failure","volume":"331","author":"Malik","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.bpj.2019.08.024_bib4","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bpj.2019.08.024_bib5","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1161\/CIRCRESAHA.116.310363","article-title":"Multi-imaging method to assay the contractile mechanical output of micropatterned human iPSC-derived cardiac myocytes","volume":"120","author":"Ribeiro","year":"2017","journal-title":"Circ. Res"},{"key":"10.1016\/j.bpj.2019.08.024_bib6","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.yjmcc.2017.12.002","article-title":"Hypertrophic cardiomyopathy-linked mutation in troponin T causes myofibrillar disarray and pro-arrhythmic action potential changes in human iPSC cardiomyocytes","volume":"114","author":"Wang","year":"2018","journal-title":"J.\u00a0Mol. Cell. Cardiol"},{"key":"10.1016\/j.bpj.2019.08.024_bib7","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s00395-016-0587-9","article-title":"Stiff matrix induces switch to pure \u03b2-cardiac myosin heavy chain expression in human ESC-derived cardiomyocytes","volume":"111","author":"Weber","year":"2016","journal-title":"Basic Res. Cardiol"},{"key":"10.1016\/j.bpj.2019.08.024_bib8","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1007\/s00210-017-1422-z","article-title":"Frequency-dependent effects of omecamtiv mecarbil on cell shortening of isolated canine ventricular cardiomyocytes","volume":"390","author":"Horv\u00e1th","year":"2017","journal-title":"Naunyn Schmiedebergs Arch. Pharmacol"},{"key":"10.1016\/j.bpj.2019.08.024_bib9","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1196\/annals.1380.026","article-title":"Cardiac \u03b2-adrenergic signaling: from subcellular microdomains to heart failure","volume":"1080","author":"Saucerman","year":"2006","journal-title":"Ann. N. Y. Acad. Sci"},{"key":"10.1016\/j.bpj.2019.08.024_bib10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0896-6273(00)80358-X","article-title":"cAMP-dependent regulation of cardiac L-type Ca2+ channels requires membrane targeting of PKA and phosphorylation of channel subunits","volume":"19","author":"Gao","year":"1997","journal-title":"Neuron"},{"key":"10.1016\/j.bpj.2019.08.024_bib11","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1161\/CIRCHEARTFAILURE.114.001585","article-title":"Phosphoregulation of cardiac inotropy via myosin binding protein-C during increased pacing frequency or \u03b21-adrenergic stimulation","volume":"8","author":"Tong","year":"2015","journal-title":"Circ Heart Fail"},{"key":"10.1016\/j.bpj.2019.08.024_bib12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.abb.2016.01.019","article-title":"Molecule specific effects of PKA-mediated phosphorylation on rat isolated heart and cardiac myofibrillar function","volume":"601","author":"Hanft","year":"2016","journal-title":"Arch. Biochem. Biophys"},{"key":"10.1016\/j.bpj.2019.08.024_bib13","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1161\/01.RES.76.6.1028","article-title":"Cardiac troponin I phosphorylation increases the rate of cardiac muscle relaxation","volume":"76","author":"Zhang","year":"1995","journal-title":"Circ. Res"},{"key":"10.1016\/j.bpj.2019.08.024_bib14","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.yjmcc.2015.02.014","article-title":"\u03b2-adrenergic effects on cardiac myofilaments and contraction in an integrated rabbit ventricular myocyte model","volume":"81","author":"Negroni","year":"2015","journal-title":"J.\u00a0Mol. Cell. Cardiol"},{"key":"10.1016\/j.bpj.2019.08.024_bib15","doi-asserted-by":"crossref","first-page":"H2092","DOI":"10.1152\/ajpheart.01241.2005","article-title":"Measurement of myofilament calcium sensitivity at physiological temperature in intact cardiac trabeculae","volume":"290","author":"Varian","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol"},{"key":"10.1016\/j.bpj.2019.08.024_bib16","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/1471-2121-12-18","article-title":"Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C","volume":"12","author":"Uys","year":"2011","journal-title":"BMC Cell Biol"},{"key":"10.1016\/j.bpj.2019.08.024_bib17","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1074\/jbc.M110.148684","article-title":"Cardiac troponin T, a sarcomeric AKAP, tethers protein kinase A at the myofilaments","volume":"286","author":"Sumandea","year":"2011","journal-title":"J.\u00a0Biol. Chem"},{"key":"10.1016\/j.bpj.2019.08.024_bib18","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1161\/01.RES.0000145361.50017.aa","article-title":"Sustained \u03b21-adrenergic stimulation modulates cardiac contractility by Ca2+\/calmodulin kinase signaling pathway","volume":"95","author":"Wang","year":"2004","journal-title":"Circ. Res"},{"key":"10.1016\/j.bpj.2019.08.024_bib19","doi-asserted-by":"crossref","first-page":"e0160605","DOI":"10.1371\/journal.pone.0160605","article-title":"In\u00a0vivo cannulation methods for cardiomyocytes isolation from heart disease models","volume":"11","author":"Jian","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.bpj.2019.08.024_bib20","doi-asserted-by":"crossref","first-page":"12923","DOI":"10.1073\/pnas.0600137103","article-title":"Systems analysis of PKA-mediated phosphorylation gradients in live cardiac myocytes","volume":"103","author":"Saucerman","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2019.08.024_bib21","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1113\/expphysiol.2009.052126","article-title":"The rate of loss of T-tubules in cultured adult ventricular myocytes is species dependent","volume":"95","author":"Pavlovi\u0107","year":"2010","journal-title":"Exp. Physiol"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349519307416?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349519307416?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:40:05Z","timestamp":1757781605000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349519307416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["S0006349519307416"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2019.08.024","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/636852","asserted-by":"object"}]},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Microwell Cell Capture Device Reveals Variable Response to Dobutamine in Isolated Cardiomyocytes","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2019.08.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Biophysical Society.","name":"copyright","label":"Copyright"}]}}