{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:03:21Z","timestamp":1790787801669,"version":"4.1.0"},"reference-count":90,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":384,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.bpj.2020.07.043","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T12:05:59Z","timestamp":1599048359000},"page":"1683-1697","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":123,"title":["Piezo1 Forms Specific, Functionally Important Interactions with Phosphoinositides and Cholesterol"],"prefix":"10.1016","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3956-3284","authenticated-orcid":false,"given":"Amanda","family":"Buyan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles D.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0343-7796","authenticated-orcid":false,"given":"Jonathan","family":"Barnoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah S.M.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Martinac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siewert J.","family":"Marrink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6324-442X","authenticated-orcid":false,"given":"Ben","family":"Corry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2020.07.043_bib1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1126\/science.1193270","article-title":"Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels","volume":"330","author":"Coste","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.bpj.2020.07.043_bib2","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1126\/science.aau6324","article-title":"PIEZOs mediate neuronal sensing of blood pressure and the baroreceptor reflex","volume":"362","author":"Zeng","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.bpj.2020.07.043_bib3","doi-asserted-by":"crossref","first-page":"e21074","DOI":"10.7554\/eLife.21074","article-title":"Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes","volume":"6","author":"Servin-Vences","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.bpj.2020.07.043_bib4","doi-asserted-by":"crossref","first-page":"10347","DOI":"10.1073\/pnas.1409233111","article-title":"Piezo1, a mechanically activated ion channel, is required for vascular development in mice","volume":"111","author":"Ranade","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2020.07.043_bib5","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nature13701","article-title":"Piezo1 integration of vascular architecture with physiological force","volume":"515","author":"Li","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib6","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.neuropharm.2019.02.015","article-title":"Mechanosensitive meningeal nociception via Piezo channels: implications for pulsatile pain in migraine?","volume":"149","author":"Mikhailov","year":"2019","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.bpj.2020.07.043_bib7","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fnmol.2019.00178","article-title":"PIEZO1 is selectively expressed in small diameter mouse DRG neurons distinct from neurons strongly expressing TRPV1","volume":"12","author":"Wang","year":"2019","journal-title":"Front. Mol. Neurosci"},{"key":"10.1016\/j.bpj.2020.07.043_bib8","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1038\/ncomms2899","article-title":"Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO1 ion channels","volume":"4","author":"Albuisson","year":"2013","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib9","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1182\/blood-2012-04-422253","article-title":"Mutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis","volume":"120","author":"Zarychanski","year":"2012","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2020.07.043_bib10","doi-asserted-by":"crossref","first-page":"8329","DOI":"10.1038\/ncomms9329","article-title":"Impaired PIEZO1 function in patients with a novel autosomal recessive congenital lymphatic dysplasia","volume":"6","author":"Lukacs","year":"2015","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib11","doi-asserted-by":"crossref","first-page":"E1162","DOI":"10.1073\/pnas.1219777110","article-title":"Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO1","volume":"110","author":"Bae","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2020.07.043_bib12","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1182\/blood-2017-05-786004","article-title":"Novel mechanisms of PIEZO1 dysfunction in hereditary xerocytosis","volume":"130","author":"Glogowska","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2020.07.043_bib13","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/nature13419","article-title":"Membrane proteins bind lipids selectively to modulate their structure and function","volume":"510","author":"Laganowsky","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib14","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.bbamem.2004.05.012","article-title":"How lipids affect the activities of integral membrane proteins","volume":"1666","author":"Lee","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2020.07.043_bib15","doi-asserted-by":"crossref","first-page":"5775","DOI":"10.1021\/acs.chemrev.8b00451","article-title":"Emerging diversity in lipid-protein interactions","volume":"119","author":"Corradi","year":"2019","journal-title":"Chem. Rev"},{"key":"10.1016\/j.bpj.2020.07.043_bib16","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.celrep.2017.09.029","article-title":"Omega-3 fatty acids modulate TRPV4 function through plasma membrane remodeling","volume":"21","author":"Caires","year":"2017","journal-title":"Cell Rep"},{"key":"10.1016\/j.bpj.2020.07.043_bib17","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/nsb827","article-title":"Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating","volume":"9","author":"Perozo","year":"2002","journal-title":"Nat. Struct. Biol"},{"key":"10.1016\/j.bpj.2020.07.043_bib18","doi-asserted-by":"crossref","first-page":"8770","DOI":"10.1073\/pnas.1200051109","article-title":"Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS","volume":"109","author":"Nomura","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2020.07.043_bib19","doi-asserted-by":"crossref","first-page":"12239","DOI":"10.1021\/bi0509649","article-title":"Assessment of potential stimuli for mechano-dependent gating of MscL: effects of pressure, tension, and lipid headgroups","volume":"44","author":"Moe","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2020.07.043_bib20","doi-asserted-by":"crossref","first-page":"E6955","DOI":"10.1073\/pnas.1514138112","article-title":"Tissue mechanics govern the rapidly adapting and symmetrical response to touch","volume":"112","author":"Eastwood","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2020.07.043_bib21","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/368265a0","article-title":"A large-conductance mechanosensitive channel in E.\u00a0coli encoded by mscL alone","volume":"368","author":"Sukharev","year":"1994","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib22","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0006-3495(02)75169-2","article-title":"Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes","volume":"83","author":"Sukharev","year":"2002","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2020.07.043_bib23","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1126\/science.1261512","article-title":"K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac","volume":"347","author":"Dong","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.bpj.2020.07.043_bib24","doi-asserted-by":"crossref","first-page":"3614","DOI":"10.1073\/pnas.1320768111","article-title":"Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K+ channels","volume":"111","author":"Brohawn","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2020.07.043_bib25","doi-asserted-by":"crossref","first-page":"e41844","DOI":"10.7554\/eLife.41844","article-title":"OSCA\/TMEM63 are an evolutionarily conserved family of mechanically activated ion channels","volume":"7","author":"Murthy","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.bpj.2020.07.043_bib26","doi-asserted-by":"crossref","first-page":"183203","DOI":"10.1016\/j.bbamem.2020.183203","article-title":"Membrane stiffness is one of the key determinants of E.\u00a0coli MscS channel mechanosensitivity","volume":"1862","author":"Xue","year":"2020","journal-title":"Biochim. Biophys. Acta Biomembr"},{"key":"10.1016\/j.bpj.2020.07.043_bib27","doi-asserted-by":"crossref","first-page":"10366","DOI":"10.1038\/ncomms10366","article-title":"Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension","volume":"7","author":"Cox","year":"2016","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib28","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.celrep.2016.10.033","article-title":"Piezo1 channels are inherently mechanosensitive","volume":"17","author":"Syeda","year":"2016","journal-title":"Cell Rep"},{"key":"10.1016\/j.bpj.2020.07.043_bib29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.4161\/chan.21064","article-title":"Gating the mechanical channel Piezo1: a comparison between whole-cell and patch recording","volume":"6","author":"Gottlieb","year":"2012","journal-title":"Channels (Austin)"},{"key":"10.1016\/j.bpj.2020.07.043_bib30","doi-asserted-by":"crossref","first-page":"3520","DOI":"10.1038\/ncomms4520","article-title":"Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch","volume":"5","author":"Poole","year":"2014","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib31","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/s42003-019-0514-3","article-title":"Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca2+ flickers","volume":"2","author":"Ellefsen","year":"2019","journal-title":"Commun. Biol"},{"key":"10.1016\/j.bpj.2020.07.043_bib32","article-title":"Tethering Piezo channels to the actin cytoskeleton for mechanogating via the E-cadherin-\u03b2-catenin mechanotransduction complex","author":"Wang","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.bpj.2020.07.043_bib33","doi-asserted-by":"crossref","first-page":"20180221","DOI":"10.1098\/rstb.2018.0221","article-title":"The plasma membrane as a mechanochemical transducer","volume":"374","author":"Le Roux","year":"2019","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci"},{"key":"10.1016\/j.bpj.2020.07.043_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.celrep.2019.08.075","article-title":"Biophysical principles of\u00a0ion-channel-mediated mechanosensory transduction","volume":"29","author":"Cox","year":"2019","journal-title":"Cell Rep"},{"key":"10.1016\/j.bpj.2020.07.043_bib35","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1016\/j.cell.2018.09.054","article-title":"Cell membranes resist flow","volume":"175","author":"Shi","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.bpj.2020.07.043_bib36","doi-asserted-by":"crossref","first-page":"e33660","DOI":"10.7554\/eLife.33660","article-title":"Structure-based membrane dome mechanism for Piezo mechanosensitivity","volume":"6","author":"Guo","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.bpj.2020.07.043_bib37","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nature25453","article-title":"Structure of the mechanically activated ion channel Piezo1","volume":"554","author":"Saotome","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib38","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nature25743","article-title":"Structure and mechanogating mechanism of the Piezo1 channel","volume":"554","author":"Zhao","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib39","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.celrep.2019.12.040","article-title":"Inactivation kinetics and mechanical gating of Piezo1 ion channels depend on subdomains within the cap","volume":"30","author":"Lewis","year":"2020","journal-title":"Cell Rep"},{"key":"10.1016\/j.bpj.2020.07.043_bib40","doi-asserted-by":"crossref","first-page":"4503","DOI":"10.1038\/s41467-019-12501-1","article-title":"A mechanism for the activation of the mechanosensitive Piezo1 channel by the small molecule Yoda1","volume":"10","author":"Botello-Smith","year":"2019","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib41","article-title":"Disruption of membrane cholesterol organization impairs the concerted activity of PIEZO1 channel clusters","author":"Ridone","year":"2019","journal-title":"bioRxiv"},{"key":"10.1016\/j.bpj.2020.07.043_bib42","doi-asserted-by":"crossref","first-page":"8512","DOI":"10.1038\/ncomms9512","article-title":"Membrane stiffening by STOML3 facilitates mechanosensation in sensory neurons","volume":"6","author":"Qi","year":"2015","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib43","doi-asserted-by":"crossref","first-page":"ra15","DOI":"10.1126\/scisignal.2005667","article-title":"Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides","volume":"8","author":"Borbiro","year":"2015","journal-title":"Sci. Signal"},{"key":"10.1016\/j.bpj.2020.07.043_bib44","doi-asserted-by":"crossref","first-page":"e32346","DOI":"10.7554\/eLife.32346","article-title":"Myotubularin related protein-2 and its phospholipid substrate PIP2 control Piezo2-mediated mechanotransduction in peripheral sensory neurons","volume":"7","author":"Narayanan","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.bpj.2020.07.043_bib45","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1038\/s41467-018-04436-w","article-title":"Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation","volume":"9","author":"Tsuchiya","year":"2018","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib46","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/nature10812","article-title":"Piezo proteins are pore-forming subunits of mechanically activated channels","volume":"483","author":"Coste","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib47","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The protein Data Bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bpj.2020.07.043_bib48","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0076-6879(03)74020-8","article-title":"Modeller: generation and refinement of homology-based protein structure models","volume":"374","author":"Fiser","year":"2003","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.bpj.2020.07.043_bib49","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"10.1016\/j.bpj.2020.07.043_bib50","doi-asserted-by":"crossref","first-page":"7812","DOI":"10.1021\/jp071097f","article-title":"The MARTINI force field: coarse grained model for biomolecular simulations","volume":"111","author":"Marrink","year":"2007","journal-title":"J.\u00a0Phys. Chem. B"},{"key":"10.1016\/j.bpj.2020.07.043_bib51","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1021\/ct300646g","article-title":"Improved parameters for the martini coarse-grained protein force field","volume":"9","author":"de Jong","year":"2013","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2020.07.043_bib52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cpc.2015.09.014","article-title":"Martini straight: boosting performance using a shorter cutoff and GPUs","volume":"199","author":"de Jong","year":"2016","journal-title":"Comput. Phys. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib53","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1063\/1.448118","article-title":"Molecular dynamics with coupling to an external bath","volume":"81","author":"Berendsen","year":"1984","journal-title":"J.\u00a0Chem. Phys"},{"key":"10.1016\/j.bpj.2020.07.043_bib54","doi-asserted-by":"crossref","first-page":"014101","DOI":"10.1063\/1.2408420","article-title":"Canonical sampling through velocity rescaling","volume":"126","author":"Bussi","year":"2007","journal-title":"J.\u00a0Chem. Phys"},{"key":"10.1016\/j.bpj.2020.07.043_bib55","first-page":"31","article-title":"A smooth particle mesh Ewald method","volume":"102","author":"Essman","year":"1995","journal-title":"J.\u00a0Chem. Phys"},{"key":"10.1016\/j.bpj.2020.07.043_bib56","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1021\/ct9002114","article-title":"Combining an elastic network with a coarse-grained molecular force field: structure, dynamics, and intermolecular recognition","volume":"5","author":"Periole","year":"2009","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2020.07.043_bib57","doi-asserted-by":"crossref","first-page":"4931","DOI":"10.1007\/s00894-013-1991-9","article-title":"Conformational flexibility of the leucine binding protein examined by protein domain coarse-grained molecular dynamics","volume":"19","author":"Siuda","year":"2013","journal-title":"J.\u00a0Mol. Model"},{"key":"10.1016\/j.bpj.2020.07.043_bib58","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1021\/acs.jctc.5b00209","article-title":"Computational lipidomics with insane: a versatile tool for generating custom membranes for molecular simulations","volume":"11","author":"Wassenaar","year":"2015","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2020.07.043_bib59","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1021\/acscentsci.8b00143","article-title":"Lipid-protein interactions are unique fingerprints for membrane proteins","volume":"4","author":"Corradi","year":"2018","journal-title":"ACS Cent. Sci"},{"key":"10.1016\/j.bpj.2020.07.043_bib60","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.chemphyslip.2013.02.001","article-title":"Free energy of WALP23 dimer association in DMPC, DPPC, and DOPC bilayers","volume":"169","author":"Castillo","year":"2013","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.bpj.2020.07.043_bib61","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1093\/bioinformatics\/btt055","article-title":"GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit","volume":"29","author":"Pronk","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.bpj.2020.07.043_bib62","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"J.\u00a0Mol. Graph"},{"key":"10.1016\/j.bpj.2020.07.043_bib63","first-page":"257","article-title":"Computational \u2018microscopy\u2019 of cellular membranes","volume":"129","author":"Ing\u00f3lfsson","year":"2016","journal-title":"J.\u00a0Cell Sci"},{"key":"10.1016\/j.bpj.2020.07.043_bib64","doi-asserted-by":"crossref","first-page":"14554","DOI":"10.1021\/ja507832e","article-title":"Lipid organization of the plasma membrane","volume":"136","author":"Ing\u00f3lfsson","year":"2014","journal-title":"J.\u00a0Am. Chem. Soc"},{"key":"10.1016\/j.bpj.2020.07.043_bib65","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/nature15247","article-title":"Architecture of the mammalian mechanosensitive Piezo1 channel","volume":"527","author":"Ge","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib66","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/s41467-019-09055-7","article-title":"Dietary fatty acids fine-tune Piezo1 mechanical response","volume":"10","author":"Romero","year":"2019","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib67","article-title":"A piezo1 open state reveals a multi-fenestrated ion permeation pathway","author":"Wenjuan","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.bpj.2020.07.043_bib68","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1182\/blood-2013-02-482489","article-title":"Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1","volume":"121","author":"Andolfo","year":"2013","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2020.07.043_bib69","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1038\/s41467-017-01712-z","article-title":"A protein interaction mechanism for suppressing the mechanosensitive Piezo channels","volume":"8","author":"Zhang","year":"2017","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib70","doi-asserted-by":"crossref","DOI":"10.1002\/pbc.26444","article-title":"Hemoglobin C trait accentuates erythrocyte dehydration in hereditary xerocytosis","volume":"64","author":"Yang","year":"2017","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.bpj.2020.07.043_bib71","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1038\/s41467-018-03570-9","article-title":"A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel","volume":"9","author":"Wang","year":"2018","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib72","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/bs.ctm.2017.05.001","article-title":"Relevance of CARC and CRAC cholesterol-recognition motifs in the nicotinic acetylcholine receptor and other membrane-bound receptors","volume":"80","author":"Di Scala","year":"2017","journal-title":"Curr. Top. Membr"},{"key":"10.1016\/j.bpj.2020.07.043_bib73","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/j.bpj.2014.07.039","article-title":"A cholesterol recognition motif in human phospholipid scramblase 1","volume":"107","author":"Posada","year":"2014","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2020.07.043_bib74","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nmeth.2368","article-title":"Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells","volume":"10","author":"Hulce","year":"2013","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bpj.2020.07.043_bib75","doi-asserted-by":"crossref","first-page":"e66068","DOI":"10.1371\/journal.pone.0066068","article-title":"Identification and analysis of putative homologues of mechanosensitive channels in pathogenic protozoa","volume":"8","author":"Prole","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.bpj.2020.07.043_bib76","doi-asserted-by":"crossref","first-page":"7223","DOI":"10.1038\/ncomms8223","article-title":"Piezo1 ion channel pore properties are dictated by C-terminal region","volume":"6","author":"Coste","year":"2015","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2020.07.043_bib77","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1002\/ajmg.a.40533","article-title":"Identification of novel PIEZO1 variants using prenatal exome sequencing and correlation to ultrasound and autopsy findings of recurrent hydrops fetalis","volume":"176","author":"Datkhaeva","year":"2018","journal-title":"Am. J. Med. Genet. A"},{"key":"10.1016\/j.bpj.2020.07.043_bib78","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1002\/ajh.25058","article-title":"Multi-gene panel testing improves diagnosis and management of patients with hereditary anemias","volume":"93","author":"Russo","year":"2018","journal-title":"Am. J. Hematol"},{"key":"10.1016\/j.bpj.2020.07.043_bib79","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/ejh.13097","article-title":"Targeted next generation sequencing for the diagnosis of patients with rare congenital anemias","volume":"101","author":"Shefer Averbuch","year":"2018","journal-title":"Eur. J. Haematol"},{"key":"10.1016\/j.bpj.2020.07.043_bib80","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.3324\/haematol.2018.205328","article-title":"Clinical and biological features in PIEZO1-hereditary xerocytosis and Gardos channelopathy: a retrospective series of 126 patients","volume":"104","author":"Picard","year":"2019","journal-title":"Haematologica"},{"key":"10.1016\/j.bpj.2020.07.043_bib81","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s10689-016-9870-z","article-title":"Exome sequencing identifies potential novel candidate genes in patients with unexplained colorectal adenomatous polyposis","volume":"15","author":"Spier","year":"2016","journal-title":"Fam. Cancer"},{"key":"10.1016\/j.bpj.2020.07.043_bib82","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.yjmcc.2020.03.013","article-title":"Piezo1 is required for outflow tract and aortic valve development","volume":"143","author":"Faucherre","year":"2020","journal-title":"J.\u00a0Mol. Cell Cardiol"},{"key":"10.1016\/j.bpj.2020.07.043_bib83","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1042\/BST20190372","article-title":"Amphipathic molecules modulate PIEZO1 activity","volume":"47","author":"Cox","year":"2019","journal-title":"Biochem. Soc. Trans"},{"key":"10.1016\/j.bpj.2020.07.043_bib84","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.sbi.2018.03.015","article-title":"How lipids contribute to ion channel function, a fat perspective on direct and indirect interactions","volume":"51","author":"Cordero-Morales","year":"2018","journal-title":"Curr. Opin. Struct. Biol"},{"key":"10.1016\/j.bpj.2020.07.043_bib85","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/nsmb.3120","article-title":"The role of lipids in mechanosensation","volume":"22","author":"Pliotas","year":"2015","journal-title":"Nat. Struct. Mol. Biol"},{"key":"10.1016\/j.bpj.2020.07.043_bib86","doi-asserted-by":"crossref","first-page":"10926","DOI":"10.1021\/bi9013193","article-title":"PIP(2)-binding site in Kir channels: definition by multiscale biomolecular simulations","volume":"48","author":"Stansfeld","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2020.07.043_bib87","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/s41586-018-0325-6","article-title":"PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling","volume":"559","author":"Yen","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2020.07.043_bib88","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1021\/ja310577u","article-title":"Evidence for cardiolipin binding sites on the membrane-exposed surface of the cytochrome bc1","volume":"135","author":"Arnarez","year":"2013","journal-title":"J.\u00a0Am. Chem. Soc"},{"key":"10.1016\/j.bpj.2020.07.043_bib89","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1016\/j.bbamem.2015.03.018","article-title":"Molecular dynamics simulations of GPCR-cholesterol interaction: an emerging paradigm","volume":"1848","author":"Sengupta","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2020.07.043_bib90","article-title":"Force-dependent Piezo1 recruitment to focal adhesions regulates adhesion maturation and turnover specifically in non-transformed cells","author":"Yao","year":"2020","journal-title":"bioRxiv"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349520306809?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349520306809?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:46:55Z","timestamp":1757782015000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349520306809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":90,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["S0006349520306809"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2020.07.043","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Piezo1 Forms Specific, Functionally Important Interactions with Phosphoinositides and Cholesterol","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2020.07.043","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2020.08.030","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Biophysical Society.","name":"copyright","label":"Copyright"}]}}