{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:51:30Z","timestamp":1792511490420,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"18","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":385,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.bpj.2021.08.011","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T23:16:01Z","timestamp":1628723761000},"page":"4041-4054","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identifying crucial E-protein residues responsible for unusual stability of Zika virus envelope"],"prefix":"10.1016","volume":"120","author":[{"given":"Chinmai","family":"Pindi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkat R.","family":"Chirasani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjib","family":"Senapati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2021.08.011_bib1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/S0140-6736(16)00014-3","article-title":"Zika virus: a new global threat for 2016","volume":"387","author":"Lancet","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.bpj.2021.08.011_bib2","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1038\/emi.2016.42","article-title":"Zika virus outbreak: \u2018a perfect storm\u2019","volume":"5","author":"Ai","year":"2016","journal-title":"Emerg. Microbes Infect"},{"key":"10.1016\/j.bpj.2021.08.011_bib3","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika virus associated with microcephaly","volume":"374","author":"Mlakar","year":"2016","journal-title":"N.\u00a0Engl. J. Med"},{"key":"10.1016\/j.bpj.2021.08.011_bib4","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1056\/NEJMsr1604338","article-title":"Zika virus and birth defects--reviewing the evidence for causality","volume":"374","author":"Rasmussen","year":"2016","journal-title":"N.\u00a0Engl. J. Med"},{"key":"10.1016\/j.bpj.2021.08.011_bib5","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/S0140-6736(16)00562-6","article-title":"Guillain-Barr\u00e9 Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study","volume":"387","author":"Cao-Lormeau","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.bpj.2021.08.011_bib6","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1126\/science.aaf5316","article-title":"The 3.8\u00a0\u00c5 resolution cryo-EM structure of Zika virus","volume":"352","author":"Sirohi","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.bpj.2021.08.011_bib7","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/nature17994","article-title":"Structure of the thermally stable Zika virus","volume":"533","author":"Kostyuchenko","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2021.08.011_bib8","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nsmb.2463","article-title":"Cryo-EM structure of the mature dengue virus at 3.5-\u00c5 resolution","volume":"20","author":"Zhang","year":"2013","journal-title":"Nat. Struct. Mol. Biol"},{"key":"10.1016\/j.bpj.2021.08.011_bib35","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem"},{"key":"10.1016\/j.bpj.2021.08.011_bib9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3201\/eid2101.140894","article-title":"Detection of Zika virus in urine","volume":"21","author":"Gourinat","year":"2015","journal-title":"Emerg. Infect. Dis"},{"key":"10.1016\/j.bpj.2021.08.011_bib10","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S1473-3099(16)00138-9","article-title":"Zika virus: high infectious viral load in semen, a new sexually transmitted pathogen?","volume":"16","author":"Mansuy","year":"2016","journal-title":"Lancet Infect. Dis"},{"key":"10.1016\/j.bpj.2021.08.011_bib11","first-page":"30159","article-title":"Isolation of infectious Zika virus from saliva and prolonged viral RNA shedding in a traveller returning from the Dominican Republic to Italy, January 2016","volume":"21","author":"Barzon","year":"2016","journal-title":"Euro Surveill"},{"key":"10.1016\/j.bpj.2021.08.011_bib12","doi-asserted-by":"crossref","first-page":"7585","DOI":"10.1128\/JVI.00757-13","article-title":"Structural changes in dengue virus when exposed to a temperature of 37\u00b0C","volume":"87","author":"Fibriansah","year":"2013","journal-title":"J.\u00a0Virol"},{"key":"10.1016\/j.bpj.2021.08.011_bib13","doi-asserted-by":"crossref","first-page":"6795","DOI":"10.1073\/pnas.1304300110","article-title":"Dengue structure differs at the temperatures of its human and mosquito hosts","volume":"110","author":"Zhang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2021.08.011_bib14","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01396-16","article-title":"Zika virus is not uniquely stable at physiological temperatures compared to other flaviviruses","volume":"7","author":"Goo","year":"2016","journal-title":"MBio"},{"key":"10.1016\/j.bpj.2021.08.011_bib15","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1128\/mBio.02134-16","article-title":"Understanding zika virus stability and developing a chimeric vaccine through functional analysis","volume":"8","author":"Xie","year":"2017","journal-title":"MBio"},{"key":"10.1016\/j.bpj.2021.08.011_bib16","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/infdis\/jix473","article-title":"CD-loop extension in zika virus envelope protein key for stability and pathogenesis","volume":"216","author":"Gallichotte","year":"2017","journal-title":"J.\u00a0Infect. Dis"},{"key":"10.1016\/j.bpj.2021.08.011_bib17","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41426-017-0006-9","article-title":"A single residue in the \u03b1B helix of the E protein is critical for Zika virus thermostability","volume":"7","author":"Xie","year":"2018","journal-title":"Emerg. Microbes Infect"},{"key":"10.1016\/j.bpj.2021.08.011_bib18","doi-asserted-by":"crossref","first-page":"8411","DOI":"10.1038\/s41598-020-65288-3","article-title":"Molecular basis of differential stability and temperature sensitivity of ZIKA versus dengue virus protein shells","volume":"10","author":"Pindi","year":"2020","journal-title":"Sci. Rep"},{"key":"10.1016\/j.bpj.2021.08.011_bib19","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1016\/j.str.2006.10.003","article-title":"Stability and dynamics of virus capsids described by coarse-grained modeling","volume":"14","author":"Arkhipov","year":"2006","journal-title":"Structure"},{"key":"10.1016\/j.bpj.2021.08.011_bib20","doi-asserted-by":"crossref","first-page":"11568","DOI":"10.1038\/ncomms11568","article-title":"Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly","volume":"7","author":"Grime","year":"2016","journal-title":"Nat. Commun"},{"key":"10.1016\/j.bpj.2021.08.011_bib21","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1016\/j.bpj.2010.08.018","article-title":"Multiscale computer simulation of the immature HIV-1 virion","volume":"99","author":"Ayton","year":"2010","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2021.08.011_bib22","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.str.2014.12.019","article-title":"Nothing to sneeze at: a dynamic and integrative computational model of an influenza A virion","volume":"23","author":"Reddy","year":"2015","journal-title":"Structure"},{"key":"10.1016\/j.bpj.2021.08.011_bib23","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1016\/j.str.2016.05.014","article-title":"Pushing the envelope: dengue viral membrane coaxed into shape by molecular simulations","volume":"24","author":"Marzinek","year":"2016","journal-title":"Structure"},{"key":"10.1016\/j.bpj.2021.08.011_bib24","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.str.2015.12.011","article-title":"The role of the membrane in the structure and biophysical robustness of the dengue virion envelope","volume":"24","author":"Reddy","year":"2016","journal-title":"Structure"},{"key":"10.1016\/j.bpj.2021.08.011_bib25","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1021\/acs.jpclett.6b00517","article-title":"All-atom molecular dynamics of virus capsids as drug targets","volume":"7","author":"Perilla","year":"2016","journal-title":"J.\u00a0Phys. Chem. Lett"},{"key":"10.1016\/j.bpj.2021.08.011_bib26","doi-asserted-by":"crossref","first-page":"165101","DOI":"10.1063\/1.4897557","article-title":"All-atom molecular dynamics calculation study of entire poliovirus empty capsids in solution","volume":"141","author":"Andoh","year":"2014","journal-title":"J.\u00a0Chem. Phys"},{"key":"10.1016\/j.bpj.2021.08.011_bib27","series-title":"Current Protocols in Bioinformatics","article-title":"Comparative protein structure modeling using MODELLER","author":"Webb","year":"2016"},{"key":"10.1016\/j.bpj.2021.08.011_bib28","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1021\/ct9002114","article-title":"Combining an elastic network with a coarse-grained molecular force field: structure, dynamics, and intermolecular recognition","volume":"5","author":"Periole","year":"2009","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2021.08.011_bib29","doi-asserted-by":"crossref","first-page":"7812","DOI":"10.1021\/jp071097f","article-title":"The MARTINI force field: coarse grained model for biomolecular simulations","volume":"111","author":"Marrink","year":"2007","journal-title":"J.\u00a0Phys. Chem. B"},{"key":"10.1016\/j.bpj.2021.08.011_bib30","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1021\/acs.jctc.8b00987","article-title":"SPICA force field for lipid membranes: domain formation induced by cholesterol","volume":"15","author":"Seo","year":"2019","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2021.08.011_bib31","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1021\/acs.jctc.9b00946","article-title":"pSPICA: a coarse-grained force field for lipid membranes based on a polar water model","volume":"16","author":"Miyazaki","year":"2020","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2021.08.011_bib32","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2017205118","article-title":"Seipin accumulates and traps diacylglycerols and triglycerides in its ring-like structure","volume":"118","author":"Zoni","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2021.08.011_bib33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"10.1016\/j.bpj.2021.08.011_bib34","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"J.\u00a0Mol. Graph"},{"key":"10.1016\/j.bpj.2021.08.011_bib36","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1002\/jcc.20090","article-title":"A biomolecular force field based on the free enthalpy of hydration and solvation: the GROMOS force-field parameter sets 53A5 and 53A6","volume":"25","author":"Oostenbrink","year":"2004","journal-title":"J.\u00a0Comput. Chem"},{"key":"10.1016\/j.bpj.2021.08.011_bib37","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1021\/ct600085e","article-title":"Generalized Born model with a simple, robust molecular volume correction","volume":"3","author":"Mongan","year":"2007","journal-title":"J.\u00a0Chem. Theory Comput"},{"key":"10.1016\/j.bpj.2021.08.011_bib46","series-title":"The PyMOL molecular graphics system, Version 2.0","author":"Schr\u00f6dinger","year":"2018"},{"key":"10.1016\/j.bpj.2021.08.011_bib38","doi-asserted-by":"crossref","first-page":"e0007212","DOI":"10.1371\/journal.pntd.0007212","article-title":"Shortening of Zika virus CD-loop reduces neurovirulence while preserving antigenicity","volume":"13","author":"Dinnon Iii","year":"2019","journal-title":"PLoS Negl. Trop. Dis"},{"key":"10.1016\/j.bpj.2021.08.011_bib39","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.aaf8505","article-title":"Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection","volume":"353","author":"Stettler","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.bpj.2021.08.011_bib40","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/nature18938","article-title":"Structural basis of potent Zika-dengue virus antibody cross-neutralization","volume":"536","author":"Barba-Spaeth","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2021.08.011_bib41","doi-asserted-by":"crossref","first-page":"4287","DOI":"10.1016\/j.vaccine.2017.04.052","article-title":"Immunization of Zika virus envelope protein domain III induces specific and neutralizing immune responses against Zika virus","volume":"35","author":"Yang","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.bpj.2021.08.011_bib42","doi-asserted-by":"crossref","first-page":"307","DOI":"10.3390\/vaccines8020307","article-title":"Immunogenicity and efficacy of zika virus envelope domain III in DNA, protein, and ChAdOx1 adenoviral-vectored vaccines","volume":"8","author":"L\u00f3pez-Camacho","year":"2020","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.bpj.2021.08.011_bib43","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/j.bpj.2020.08.036","article-title":"Disulfide reduction allosterically destabilizes the \u03b2-ladder subdomain assembly within the NS1 dimer of ZIKV","volume":"119","author":"Roy","year":"2020","journal-title":"Biophys. J"},{"key":"10.1016\/j.bpj.2021.08.011_bib44","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1021\/acs.jcim.9b00809","article-title":"Identification of a potential zika virus inhibitor targeting NS5 methyltransferase using virtual screening and molecular dynamics simulations","volume":"60","author":"Santos","year":"2020","journal-title":"J.\u00a0Chem. Inf. Model"},{"key":"10.1016\/j.bpj.2021.08.011_bib45","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1021\/acs.jcim.9b00933","article-title":"Identification of zika virus NS2B-NS3 protease inhibitors by structure-based virtual screening and drug repurposing approaches","volume":"60","author":"Santos","year":"2020","journal-title":"J.\u00a0Chem. Inf. Model"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349521006573?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349521006573?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:54:33Z","timestamp":1757782473000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349521006573"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":46,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["S0006349521006573"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2021.08.011","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identifying crucial E-protein residues responsible for unusual stability of Zika virus envelope","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2021.08.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021","name":"copyright","label":"Copyright"}]}}