{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:10:16Z","timestamp":1792077016198,"version":"4.4.0"},"reference-count":112,"publisher":"Elsevier BV","issue":"21","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL089456"],"award-info":[{"award-number":["R01HL089456"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20201302"],"award-info":[{"award-number":["BK20201302"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.bpj.2022.09.040","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T10:52:17Z","timestamp":1664794337000},"page":"4033-4047","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"title":["von Willebrand factor unfolding mediates platelet deposition in a model of high-shear thrombosis"],"prefix":"10.1016","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8806-2940","authenticated-orcid":false,"given":"Mansur","family":"Zhussupbekov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"M\u00e9ndez Rojano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Tao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5430-7353","authenticated-orcid":false,"given":"James F.","family":"Antaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2022.09.040_bib1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s00018-014-1743-8","article-title":"Of von Willebrand factor and platelets","volume":"72","author":"Bryckaert","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bpj.2022.09.040_bib2","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1080\/10739680802651477","article-title":"Platelet adhesion under flow","volume":"16","author":"Ruggeri","year":"2009","journal-title":"Microcirculation"},{"key":"10.1016\/j.bpj.2022.09.040_bib3","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1016\/j.jmb.2019.02.014","article-title":"Electrostatic steering enables flow-activated von willebrand factor to bind platelet glycoprotein, revealed by single-molecule stretching and imaging","volume":"431","author":"Jiang","year":"2019","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib4","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s13239-012-0086-6","article-title":"Mechanisms of platelet capture under very high shear","volume":"3","author":"Wellings","year":"2012","journal-title":"Cardiovasc. Eng. Technol."},{"key":"10.1016\/j.bpj.2022.09.040_bib5","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1016\/j.jvs.2014.12.050","article-title":"Role of high shear rate in thrombosis","volume":"61","author":"Casa","year":"2015","journal-title":"J.\u00a0Vasc. Surg."},{"key":"10.1016\/j.bpj.2022.09.040_bib6","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1182\/blood-2014-05-378638","article-title":"von Willebrand factor, Jedi knight of the bloodstream","volume":"124","author":"Springer","year":"2014","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2022.09.040_bib7","doi-asserted-by":"crossref","first-page":"7899","DOI":"10.1073\/pnas.0608422104","article-title":"Shear-induced unfolding triggers adhesion of von Willebrand factor fibers","volume":"104","author":"Schneider","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2022.09.040_bib8","doi-asserted-by":"crossref","first-page":"L35","DOI":"10.1016\/j.bpj.2010.01.032","article-title":"Elongational flow induces the unfolding of von Willebrand factor at physiological flow rates","volume":"98","author":"Sing","year":"2010","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib9","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-00230-2","article-title":"Flow-induced elongation of von Willebrand factor precedes tension-dependent activation","volume":"8","author":"Fu","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bpj.2022.09.040_bib10","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1126\/science.1170905","article-title":"Mechanoenzymatic cleavage of the ultralarge vascular protein von Willebrand factor","volume":"324","author":"Zhang","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.bpj.2022.09.040_bib11","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.bpj.2015.12.023","article-title":"Shear-induced unfolding and enzymatic cleavage of full-Length VWF multimers","volume":"110","author":"Lippok","year":"2016","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib12","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1160\/TH13-09-0800","article-title":"The various states of von Willebrand factor and their function in physiology and pathophysiology","volume":"111","author":"Huck","year":"2014","journal-title":"Thromb. Haemostasis"},{"key":"10.1016\/j.bpj.2022.09.040_bib13","doi-asserted-by":"crossref","first-page":"041502","DOI":"10.1063\/1.5115554","article-title":"Occlusive thrombosis in arteries","volume":"3","author":"Kim","year":"2019","journal-title":"APL Bioeng"},{"key":"10.1016\/j.bpj.2022.09.040_bib14","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1038\/nm.1955","article-title":"A shear gradient\u2013dependent platelet aggregation mechanism drives thrombus formation","volume":"15","author":"Nesbitt","year":"2009","journal-title":"Nat. Med."},{"key":"10.1016\/j.bpj.2022.09.040_bib15","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1182\/blood-2006-04-011551","article-title":"Activation-independent platelet adhesion and aggregation under elevated shear stress","volume":"108","author":"Ruggeri","year":"2006","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2022.09.040_bib16","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1161\/STROKEAHA.111.628867","article-title":"Von Willebrand factor: an emerging target in stroke therapy","volume":"43","author":"De Meyer","year":"2012","journal-title":"Stroke"},{"key":"10.1016\/j.bpj.2022.09.040_bib17","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1111\/j.1538-7836.2006.01949.x","article-title":"von Willebrand factor, endothelial dysfunction, and cardiovascular disease","volume":"4","author":"Vischer","year":"2006","journal-title":"J.\u00a0Thromb. Haemostasis"},{"key":"10.1016\/j.bpj.2022.09.040_bib18","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1182\/blood-2015-08-662650","article-title":"ADAMTS13-mediated thrombolysis of t-PA-resistant occlusions in ischemic stroke in mice","volume":"127","author":"Denorme","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2022.09.040_bib19","doi-asserted-by":"crossref","first-page":"498","DOI":"10.3324\/haematol.2019.219881","article-title":"Structural analysis of ischemic stroke thrombi: histological indications for therapy resistance","volume":"105","author":"Staessens","year":"2020","journal-title":"Haematologica"},{"key":"10.1016\/j.bpj.2022.09.040_bib20","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1073\/pnas.1209905110","article-title":"Atherosclerotic geometries exacerbate pathological thrombus formation poststenosis in a von Willebrand factor-dependent manner","volume":"110","author":"Westein","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2022.09.040_bib21","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1039\/B916757A","article-title":"A microfluidics device to monitor platelet aggregation dynamics in response to strain rate micro-gradients in flowing blood","volume":"10","author":"Tovar-Lopez","year":"2010","journal-title":"Lab Chip"},{"key":"10.1016\/j.bpj.2022.09.040_bib22","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1007\/s10439-011-0296-3","article-title":"Rapid platelet accumulation leading to thrombotic occlusion","volume":"39","author":"Para","year":"2011","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib23","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.3324\/haematol.2019.235754","article-title":"Shear rate gradients promote a bi-phasic thrombus formation on weak adhesive proteins, such as fibrinogen in a von Willebrand factor-dependent manner","volume":"105","author":"Receveur","year":"2020","journal-title":"Haematologica"},{"key":"10.1016\/j.bpj.2022.09.040_bib24","article-title":"Structure of shear-induced platelet aggregated clot formed in an in\u00a0vitro arterial thrombosis model","author":"Kim","year":"2022","journal-title":"Blood Adv"},{"key":"10.1016\/j.bpj.2022.09.040_bib25","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s13239-017-0304-3","article-title":"Prediction of thrombus growth: effect of stenosis and Reynolds number","volume":"8","author":"Hosseinzadegan","year":"2017","journal-title":"Cardiovasc. Eng. Technol."},{"key":"10.1016\/j.bpj.2022.09.040_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pcbi.1005291","article-title":"A general shear-dependent model for thrombus formation","volume":"13","author":"Yazdani","year":"2017","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pcbi.1009850","article-title":"A three-dimensional multiscale model for the prediction of thrombus growth under flow with single-platelet resolution","volume":"18","author":"Shankar","year":"2022","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijengsci.2019.103206","article-title":"Simulation of thrombosis in a stenotic microchannel: the effects of vWF-enhanced shear activation of platelets","volume":"147","author":"Wu","year":"2020","journal-title":"Int. J. Eng. Sci."},{"key":"10.1016\/j.bpj.2022.09.040_bib29","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1021\/ma9028008","article-title":"Globule\u2212Stretch transitions of collapsed polymers in elongational flow fields","volume":"43","author":"Sing","year":"2010","journal-title":"Macromolecules"},{"key":"10.1016\/j.bpj.2022.09.040_bib30","article-title":"Dynamics of collapsed polymers under the simultaneous influence of elongational and shear flows","volume":"135","author":"Sing","year":"2011","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.bpj.2022.09.040_bib31","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/fld.4752","article-title":"Multiscale method based on coupled lattice-Boltzmann and Langevin-dynamics for direct simulation of nanoscale particle\/polymer suspensions in complex flows","volume":"91","author":"Liu","year":"2019","journal-title":"Int. J. Numer. Methods Fluid."},{"key":"10.1016\/j.bpj.2022.09.040_bib32","doi-asserted-by":"crossref","DOI":"10.1007\/s10439-021-02845-5","article-title":"A continuum model for the unfolding of von Willebrand factor","author":"Zhussupbekov","year":"2021","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib33","doi-asserted-by":"crossref","first-page":"42720","DOI":"10.1038\/srep42720","article-title":"Multi-constituent simulation of thrombus deposition","volume":"7","author":"Wu","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bpj.2022.09.040_bib34","article-title":"Influence of shear rate and surface chemistry on thrombus formation in micro-crevice","volume":"110397","author":"Zhussupbekov","year":"2021","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2022.09.040_bib35","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1093\/imammb\/dqq005","article-title":"Grow with the flow: a spatial-temporal model of platelet deposition and blood coagulation under flow","volume":"28","author":"Leiderman","year":"2011","journal-title":"Math. Med. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib36","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/0009-2509(91)87018-8","article-title":"Flow of a fluid\u2014solid mixture between flat plates","volume":"46","author":"Johnson","year":"1991","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.bpj.2022.09.040_bib37","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1016\/j.bpj.2020.08.041","article-title":"Clot permeability, agonist transport, and platelet binding kinetics in arterial thrombosis","volume":"119","author":"Du","year":"2020","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib38","series-title":"Mathematics, Numerics, Derivations and OpenFOAM(R). Release 7","author":"Holzmann","year":"2020"},{"key":"10.1016\/j.bpj.2022.09.040_bib39","series-title":"pimpleFoam, GitHub repository","author":"Weller","year":"2018"},{"key":"10.1016\/j.bpj.2022.09.040_bib40","series-title":"OpenFOAM Programmer\u2019s Guide v3.0.1","volume":"100","author":"Greenshields","year":"2015"},{"key":"10.1016\/j.bpj.2022.09.040_bib41","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1007\/s10439-005-2951-z","article-title":"Computational model of device-induced thrombosis and thromboembolism","volume":"33","author":"Goodman","year":"2005","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib42","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/BF02367081","article-title":"1993 Whitaker Lecture: biorheology in thrombosis research","volume":"22","author":"Hellums","year":"1994","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib43","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0006-3495(86)83535-4","article-title":"Platelet active concentration profiles near growing thrombi. A mathematical consideration","volume":"50","author":"Hubbell","year":"1986","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib44","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1016\/S0006-3495(89)82760-2","article-title":"Mathematical analysis of mural thrombogenesis. Concentration profiles of platelet-activating agents and effects of viscous shear flow","volume":"56","author":"Folie","year":"1989","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib45","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1114\/1.200","article-title":"Computational simulation of platelet deposition and activation: I. Model development and properties","volume":"27","author":"Sorensen","year":"1999","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib46","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1016\/j.bpj.2013.07.037","article-title":"Exponential size distribution of von Willebrand factor","volume":"105","author":"Lippok","year":"2013","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib47","doi-asserted-by":"crossref","first-page":"4544","DOI":"10.1021\/ma034073p","article-title":"Visualization of molecular Fluctuations near the critical point of the Coil\u2212Stretch transition in polymer elongation","volume":"36","author":"Babcock","year":"2003","journal-title":"Macromolecules"},{"key":"10.1016\/j.bpj.2022.09.040_bib48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1103\/PhysRevLett.97.138101","article-title":"Shear-flow-induced unfolding of polymeric globules","volume":"97","author":"Alexander-Katz","year":"2006","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.bpj.2022.09.040_bib49","article-title":"A mechano-reactive coarse-grained model of the blood-clotting agent von Willebrand factor","volume":"151","author":"Dong","year":"2019","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.bpj.2022.09.040_bib50","article-title":"Predicting pathological von Willebrand factor unraveling in elongational flow","volume":"1\u201313","author":"Kania","year":"2021","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib51","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1126\/science.1086070","article-title":"Observation of polymer conformation hysteresis in extensional flow","volume":"301","author":"Schroeder","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.bpj.2022.09.040_bib52","series-title":"The ParaView Guide: A Parallel Visualization Application","author":"Ayachit","year":"2015"},{"key":"10.1016\/j.bpj.2022.09.040_bib53","doi-asserted-by":"crossref","first-page":"e3595","DOI":"10.1002\/cnm.3595","article-title":"Uncertainty quantification of a thrombosis model considering the clotting assay PFA-100\u00ae","volume":"38","author":"M\u00e9ndez Rojano","year":"2022","journal-title":"Int. J. Numer. Method. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib54","doi-asserted-by":"crossref","first-page":"110275","DOI":"10.1016\/j.jbiomech.2021.110275","article-title":"A predictive multiscale model for simulating flow-induced platelet activation: correlating in\u00a0silico results with in\u00a0vitro results","volume":"117","author":"Zhang","year":"2021","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2022.09.040_bib55","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1002\/bit.24537","article-title":"Correlation of thrombosis growth rate to pathological wall shear rate during platelet accumulation","volume":"109","author":"Bark","year":"2012","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bpj.2022.09.040_bib56","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1007\/s13239-014-0180-z","article-title":"High shear thrombus formation under pulsatile and steady flow","volume":"5","author":"Casa","year":"2014","journal-title":"Cardiovasc. Eng. Technol."},{"key":"10.1016\/j.bpj.2022.09.040_bib57","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1114\/1.201","article-title":"Computational simulation of platelet deposition and activation: II. Results for Poiseuille flow over collagen","volume":"27","author":"Sorensen","year":"1999","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib58","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1161\/01.CIR.23.4.578","article-title":"Plasma heparin levels in normal man","volume":"23","author":"Engelberg","year":"1961","journal-title":"Circulation"},{"key":"10.1016\/j.bpj.2022.09.040_bib59","series-title":"PFA-100 Syst","article-title":"PFA-100 system","author":"Siemens Healthineers","year":"2021"},{"key":"10.1016\/j.bpj.2022.09.040_bib60","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1055\/s-0029-1145254","article-title":"Clinical utility of the PFA-100","volume":"34","author":"Favaloro","year":"2008","journal-title":"Semin. Thromb. Hemost."},{"key":"10.1016\/j.bpj.2022.09.040_bib61","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1046\/j.1365-2257.2002.00451.x","article-title":"The PFA-100 \u00ae : a potential rapid screening tool for the assessment of platelet dysfunction","volume":"24","author":"Harrison","year":"2002","journal-title":"Clin. Lab. Haematol."},{"key":"10.1016\/j.bpj.2022.09.040_bib62","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1055\/s-0032-1313612","article-title":"Description of an in\u00a0vitro platelet function analyzer - PFA-100","volume":"21","author":"Kundu","year":"1995","journal-title":"Semin. Thromb. Hemost."},{"key":"10.1016\/j.bpj.2022.09.040_bib63","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1093\/clinchem\/40.9.1827","article-title":"Evaluation of platelet function by PFA-100tm","volume":"40","author":"Kundu","year":"1994","journal-title":"Clin. Chem."},{"key":"10.1016\/j.bpj.2022.09.040_bib64","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.athoracsur.2008.10.027","article-title":"Platelet dysfunction in outpatients with left ventricular assist devices","volume":"87","author":"Steinlechner","year":"2009","journal-title":"Ann. Thorac. Surg."},{"key":"10.1016\/j.bpj.2022.09.040_bib65","doi-asserted-by":"crossref","first-page":"043401","DOI":"10.1063\/1.4945604","article-title":"Microfluidic extensional rheometry using stagnation point flow","volume":"10","author":"Haward","year":"2016","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.bpj.2022.09.040_bib66","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10404-019-2295-x","article-title":"Optimised multi-stream microfluidic designs for controlled extensional deformation","volume":"23","author":"Zografos","year":"2019","journal-title":"Microfluid. Nanofluidics"},{"key":"10.1016\/j.bpj.2022.09.040_bib67","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s12195-017-0503-x","article-title":"Thrombi produced in stagnation point flows have a core\u2013shell structure","volume":"10","author":"Herbig","year":"2017","journal-title":"Cell. Mol. Bioeng."},{"key":"10.1016\/j.bpj.2022.09.040_bib68","series-title":"The Saint-Chopra Guide to Inpatient Medicine","first-page":"329","article-title":"Thrombocytopenia","author":"Devata","year":"2018"},{"key":"10.1016\/j.bpj.2022.09.040_bib69","series-title":"Hemostasis and Thrombosis: Basic Principles and Clinical Practice","first-page":"825","article-title":"von Willebrand disease: diagnosis, classification, and treatment","author":"Sadler","year":"2001"},{"key":"10.1016\/j.bpj.2022.09.040_bib70","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1016\/S0006-3495(01)76085-7","article-title":"Surface-mediated control of blood coagulation: the role of binding site densities and platelet deposition","volume":"80","author":"Kuharsky","year":"2001","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib71","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/jth.14653","article-title":"A mathematical model of coagulation under flow identifies factor V as a modifier of thrombin generation in hemophilia A","volume":"18","author":"Link","year":"2020","journal-title":"J.\u00a0Thromb. Haemostasis"},{"key":"10.1016\/j.bpj.2022.09.040_bib72","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1161\/ATVBAHA.120.314648","article-title":"Computationally driven discovery in coagulation","volume":"41","author":"Link","year":"2021","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib73","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.jtbi.2008.04.015","article-title":"A model for the formation, growth, and lysis of clots in quiescent plasma. A comparison between the effects of antithrombin III deficiency and protein C deficiency","volume":"253","author":"Anand","year":"2008","journal-title":"J.\u00a0Theor. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib74","first-page":"291","article-title":"A mathematical model for in\u00a0vitro coagulation of blood: role of platelet count and inhibition","volume":"42","author":"Susree","year":"2017","journal-title":"Sadhana Acad. Proc. Eng. Sci."},{"key":"10.1016\/j.bpj.2022.09.040_bib75","doi-asserted-by":"crossref","first-page":"110393","DOI":"10.1016\/j.jbiomech.2021.110393","article-title":"Refining a numerical model for device-induced thrombosis and investigating the effects of non-Newtonian blood models","volume":"120","author":"Yang","year":"2021","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2022.09.040_bib76","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/imammb\/21.4.293","article-title":"Platelet-wall interactions in continuum models of platelet thrombosis: Formulation and numerical solution","volume":"21","author":"Fogelson","year":"2004","journal-title":"Math. Med. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib77","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1093\/imammb\/dqx001","article-title":"A Two-phase mixture model of platelet aggregation","volume":"35","author":"Du","year":"2018","journal-title":"Math. Med. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib78","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep38025","article-title":"High fidelity computational simulation of thrombus formation in Thoratec HeartMate II continuous flow ventricular assist device","volume":"6","author":"Wu","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bpj.2022.09.040_bib79","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/aor.13949","article-title":"Multi-constituent simulation of thrombus formation at LVAD inlet cannula connection: importance of Virchow\u2019s triad","volume":"45","author":"M\u00e9ndez Rojano","year":"2021","journal-title":"Artif. Organs"},{"key":"10.1016\/j.bpj.2022.09.040_bib80","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1016\/j.bpj.2009.12.4331","article-title":"A multiscale model of venous thrombus formation with surface-mediated control of blood coagulation cascade","volume":"98","author":"Xu","year":"2010","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib81","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1016\/j.bpj.2019.01.040","article-title":"In\u00a0vitro measurement and modeling of platelet adhesion on VWF-coated surfaces in channel flow","volume":"116","author":"Qi","year":"2019","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib82","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1182\/blood-2011-10-388140","article-title":"Multiscale prediction of patient-specific platelet function under flow","volume":"120","author":"Flamm","year":"2012","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2022.09.040_bib83","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00285-015-0891-2","article-title":"Modelling of platelet\u2013fibrin clot formation in flow with a DPD\u2013PDE method","volume":"72","author":"Tosenberger","year":"2016","journal-title":"J.\u00a0Math. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib84","doi-asserted-by":"crossref","first-page":"110349","DOI":"10.1016\/j.jbiomech.2021.110349","article-title":"Mechanobiology of shear-induced platelet aggregation leading to occlusive arterial thrombosis: a multiscale in\u00a0silico analysis","volume":"120","author":"Liu","year":"2021","journal-title":"J.\u00a0Biomech."},{"key":"10.1016\/j.bpj.2022.09.040_bib85","doi-asserted-by":"crossref","first-page":"e1007709","DOI":"10.1371\/journal.pcbi.1007709","article-title":"A three-dimensional phase-field model for multiscale modeling of thrombus biomechanics in blood vessels","volume":"16","author":"Zheng","year":"2020","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.bpj.2022.09.040_bib86","doi-asserted-by":"crossref","first-page":"20170441","DOI":"10.1098\/rsif.2017.0441","article-title":"Model predictions of deformation, embolization and permeability of partially obstructive blood clots under variable shear flow","volume":"14","author":"Xu","year":"2017","journal-title":"J.\u00a0R. Soc. Interface"},{"key":"10.1016\/j.bpj.2022.09.040_bib87","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/fluids2030035","article-title":"A short review of advances in the modelling of blood rheology and clot formation","volume":"2","author":"Anand","year":"2017","journal-title":"Fluid"},{"key":"10.1016\/j.bpj.2022.09.040_bib88","doi-asserted-by":"crossref","first-page":"100349","DOI":"10.1016\/j.cobme.2021.100349","article-title":"Mathematical and computational modeling of device-induced thrombosis","volume":"20","author":"Manning","year":"2021","journal-title":"Curr. Opin. Biomed. Eng."},{"key":"10.1016\/j.bpj.2022.09.040_bib89","doi-asserted-by":"crossref","first-page":"S12","DOI":"10.1016\/j.thromres.2014.03.005","article-title":"An overview of mathematical modeling of thrombus formation under flow","volume":"133","author":"Leiderman","year":"2014","journal-title":"Thromb. Res."},{"key":"10.1016\/j.bpj.2022.09.040_bib90","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.plrev.2018.02.005","article-title":"Modeling thrombosis in\u00a0silico: Frontiers, challenges, unresolved problems and milestones","volume":"26\u201327","author":"Belyaev","year":"2018","journal-title":"Phys. Life Rev."},{"key":"10.1016\/j.bpj.2022.09.040_bib91","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.ymeth.2020.01.011","article-title":"In silico trials: verification, validation and uncertainty quantification of predictive models used in the regulatory evaluation of biomedical products","volume":"185","author":"Viceconti","year":"2021","journal-title":"Methods"},{"key":"10.1016\/j.bpj.2022.09.040_bib92","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1182\/blood.2021012595","article-title":"Single-molecule imaging of von Willebrand factor reveals tension-dependent self-association","volume":"138","author":"Fu","year":"2021","journal-title":"Blood"},{"key":"10.1016\/j.bpj.2022.09.040_bib93","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.thromres.2014.03.024","article-title":"High shear dependent von willebrand factor self-assembly fostered by platelet interaction and controlled by ADAMTS13","volume":"133","author":"Kragh","year":"2014","journal-title":"Thromb. Res."},{"key":"10.1016\/j.bpj.2022.09.040_bib94","first-page":"1","article-title":"Blood-clotting-inspired reversible polymer-colloid composite assembly in flow","volume":"4","author":"Chen","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bpj.2022.09.040_bib95","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1161\/01.RES.0000267878.97021.ab","article-title":"Adhesion mechanisms in platelet function","volume":"100","author":"Ruggeri","year":"2007","journal-title":"Circ. Res."},{"key":"10.1016\/j.bpj.2022.09.040_bib96","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/BF02120313","article-title":"A calculation of the viscous force exerted by a flowing fluid on a dense swarm of particles","volume":"1","author":"Brinkman","year":"1949","journal-title":"Flow, Turbul. Combust."},{"key":"10.1016\/j.bpj.2022.09.040_bib97","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1063\/1.866465","article-title":"Analysis of the Brinkman equation as a model for flow in porous media","volume":"30","author":"Durlofsky","year":"1987","journal-title":"Phys. Fluids"},{"key":"10.1016\/j.bpj.2022.09.040_bib98","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1016\/S0006-3495(94)80689-7","article-title":"Dynamics of platelet glycoprotein IIb-IIIa receptor expression and fibrinogen binding. I. Quantal activation of platelet subpopulations varies with adenosine diphosphate concentration","volume":"67","author":"Frojmovic","year":"1994","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib99","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/245103a0","article-title":"Effect of blood flow velocity on growth rate of platelet thrombi","volume":"245","author":"Richardson","year":"1973","journal-title":"Nature"},{"key":"10.1016\/j.bpj.2022.09.040_bib100","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF01403622","article-title":"Effect of leucotrienes C4, D4, prostacyclin and thromboxane A2 on isolated human cerebral arteries","volume":"62","author":"Von Holst","year":"1982","journal-title":"Acta Neurochir."},{"key":"10.1016\/j.bpj.2022.09.040_bib101","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.ijengsci.2013.12.001","article-title":"A numerical study of blood flow using mixture theory","volume":"76","author":"Wu","year":"2014","journal-title":"Int. J. Eng. Sci."},{"key":"10.1016\/j.bpj.2022.09.040_bib102","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ijnonlinmec.2013.11.006","article-title":"On the coefficients of the interaction forces in a two-phase flow of a fluid infused with particles","volume":"59","author":"Wu","year":"2014","journal-title":"Int. J. Non Lin. Mech."},{"key":"10.1016\/j.bpj.2022.09.040_bib103","doi-asserted-by":"crossref","first-page":"38266","DOI":"10.1074\/jbc.M607123200","article-title":"Solution structure of human von Willebrand factor studied using small angle neutron scattering\u2217","volume":"281","author":"Singh","year":"2006","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2022.09.040_bib104","series-title":"Webplotdigitizer","author":"Rohatgi","year":"2021"},{"key":"10.1016\/j.bpj.2022.09.040_bib105","first-page":"362","article-title":"In\u00a0vivo and in\u00a0vitro studies of the inhibitory effect of propofol on human platelet aggregation","volume":"88","author":"Aoki","year":"1998","journal-title":"J.\u00a0Am. Soc. Anesthesiol."},{"key":"10.1016\/j.bpj.2022.09.040_bib106","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1039\/b717824g","article-title":"A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood","volume":"8","author":"Neeves","year":"2008","journal-title":"Lab Chip"},{"key":"10.1016\/j.bpj.2022.09.040_bib107","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1161\/01.CIR.104.2.140","article-title":"Platelet glycoprotein iiia pla polymorphism, fibrinogen, and platelet aggregability the framingham heart study","volume":"104","author":"Feng","year":"2001","journal-title":"Circulation"},{"key":"10.1016\/j.bpj.2022.09.040_bib108","series-title":"Platelets: Pathophysiology and Antiplatelet Drug Therapy","author":"Weiss","year":"1982"},{"key":"10.1016\/j.bpj.2022.09.040_bib109","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1055\/s-0038-1649646","article-title":"Antiphospholipid antibodies enhance thrombin-induced platelet activation and thromboxane formation","volume":"70","author":"Martinuzzo","year":"1993","journal-title":"Thromb. Haemostasis"},{"key":"10.1016\/j.bpj.2022.09.040_bib110","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1042\/bj2430809","article-title":"A diacylglycerol kinase inhibitor, R59022, potentiates secretion by and aggregation of thrombin-stimulated human platelets","volume":"243","author":"Nunn","year":"1987","journal-title":"Biochem.\u00a0J."},{"key":"10.1016\/j.bpj.2022.09.040_bib111","series-title":"Chemistry, Biochemistry, and Pharmacological Activity of Prostanoids","author":"Jones","year":"1979"},{"key":"10.1016\/j.bpj.2022.09.040_bib112","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1172\/JCI110824","article-title":"Reduced platelet thromboxane formation in uremia. Evidence for a functional cyclooxygenase defect","volume":"71","author":"Remuzzi","year":"1983","journal-title":"J.\u00a0Clin. Invest."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349522008177?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349522008177?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T17:02:34Z","timestamp":1757782954000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349522008177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":112,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["S0006349522008177"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2022.09.040","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"von Willebrand factor unfolding mediates platelet deposition in a model of high-shear thrombosis","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2022.09.040","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Biophysical Society.","name":"copyright","label":"Copyright"}]}}