{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:01:00Z","timestamp":1777424460893,"version":"3.51.4"},"reference-count":85,"publisher":"Elsevier BV","issue":"16","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":384,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100006940","name":"The University of Mississippi Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006940","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["0923184"],"award-info":[{"award-number":["0923184"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2024,8]]},"DOI":"10.1016\/j.bpj.2024.06.015","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T19:01:11Z","timestamp":1718737271000},"page":"2506-2521","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hydrodynamic and thermodynamic analysis of\u00a0PEGylated human serum albumin"],"prefix":"10.1016","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6885-7508","authenticated-orcid":false,"given":"John J.","family":"Correia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter F.","family":"Stafford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Erlandsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Cole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanduni H.","family":"Premathilaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dragan","family":"Isailovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John David","family":"Dignam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bpj.2024.06.015_bib1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0005-2795(79)90110-7","article-title":"Preparation and properties of polyethylene glycol-trypsin adducts","volume":"578","author":"Abuchowski","year":"1979","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2024.06.015_bib2","first-page":"1077","article-title":"Immunosuppressive properties and circulating life of Achromobacter glutaminase-asparaginase covalently attached to polyethylene glycol in man","volume":"65","author":"Abuchowski","year":"1981","journal-title":"Cancer Treat Rep."},{"key":"10.1016\/j.bpj.2024.06.015_bib3","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1016\/S0021-9258(17)40292-4","article-title":"Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase","volume":"252","author":"Abuchowski","year":"1977","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib4","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1177\/0192623319852300","article-title":"Nonclinical Safety Assessment of a Long-Acting Recombinant PEGylated Factor Eight (BAY 94-9027) With a 60\u00a0kDa PEG","volume":"47","author":"Ivens","year":"2019","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.bpj.2024.06.015_bib5","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jconrel.2014.06.046","article-title":"PEG - a versatile conjugating ligand for drugs and drug delivery systems","volume":"192","author":"Kolate","year":"2014","journal-title":"J.\u00a0Control. Release"},{"key":"10.1016\/j.bpj.2024.06.015_bib6","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1002\/jssc.201901340","article-title":"Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals","volume":"43","author":"Zhang","year":"2020","journal-title":"J.\u00a0Separ. Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib7","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0169-409X(02)00026-1","article-title":"PEGylated antibodies and antibody fragments for improved therapy: a review","volume":"54","author":"Chapman","year":"2002","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.bpj.2024.06.015_bib8","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1021\/bc200478w","article-title":"Analytical measurement of PEGylated molecules","volume":"23","author":"Cheng","year":"2012","journal-title":"Bioconjugate Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib9","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/S0169-409X(03)00108-X","article-title":"Pharmacokinetic and biodistribution properties of poly(ethylene glycol)-protein conjugates","volume":"55","author":"Caliceti","year":"2003","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.bpj.2024.06.015_bib10","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1021\/mp200470c","article-title":"Effect of PEGylation on protein hydrodynamics","volume":"9","author":"Gokarn","year":"2012","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.bpj.2024.06.015_bib11","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1002\/jps.21170","article-title":"Effect of PEGylation on the solution conformation of antibody fragments","volume":"97","author":"Lu","year":"2008","journal-title":"J.\u00a0Pharmaceut. Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib12","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1177\/1352458514557986","article-title":"Peginterferon beta-1a in multiple sclerosis: 2-year results from ADVANCE","volume":"21","author":"Kieseier","year":"2015","journal-title":"Mult. Scler."},{"key":"10.1016\/j.bpj.2024.06.015_bib13","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s11894-001-0038-z","article-title":"Pegylated interferons: what role will they play in\u00a0the treatment of chronic hepatitis C?","volume":"3","author":"Shiffman","year":"2001","journal-title":"Curr. Gastroenterol. Rep."},{"key":"10.1016\/j.bpj.2024.06.015_bib14","doi-asserted-by":"crossref","first-page":"e104","DOI":"10.15190\/d.2020.1","article-title":"Artificial Blood: The History and Current Perspectives of Blood Substitutes","volume":"8","author":"Khan","year":"2020","journal-title":"Discoveries"},{"key":"10.1016\/j.bpj.2024.06.015_bib15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0301-4622(97)00079-3","article-title":"Colloid osmotic properties of modified hemoglobins: chemically cross-linked versus polyethylene glycol surface-conjugated","volume":"69","author":"Vandegriff","year":"1997","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib16","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1002\/cbic.201000291","article-title":"Reviving artificial blood: meeting the challenge of dealing with NO scavenging by hemoglobin","volume":"11","author":"Lui","year":"2010","journal-title":"Chembiochem"},{"key":"10.1016\/j.bpj.2024.06.015_bib17","doi-asserted-by":"crossref","first-page":"G1301","DOI":"10.1152\/ajpgi.00399.2004","article-title":"Binding of acellular, native and cross-linked human hemoglobins to haptoglobin: enhanced distribution and clearance in the rat","volume":"288","author":"Ship","year":"2005","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"10.1016\/j.bpj.2024.06.015_bib18","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.cbpa.2010.03.021","article-title":"Red cell substitutes from hemoglobin--do we start all over again?","volume":"14","author":"Kluger","year":"2010","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.bpj.2024.06.015_bib19","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/trf.15003","article-title":"Cross-linked hemoglobin bis-tetramers from bioorthogonal coupling do not induce vasoconstriction in the circulation","volume":"59","author":"Wang","year":"2019","journal-title":"Transfusion"},{"key":"10.1016\/j.bpj.2024.06.015_bib20","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1111\/j.1525-1594.2004.07392.x","article-title":"MP4, a new nonvasoactive polyethylene glycol-hemoglobin conjugate","volume":"28","author":"Winslow","year":"2004","journal-title":"Artif. Organs"},{"key":"10.1016\/j.bpj.2024.06.015_bib21","article-title":"Comparison of the Pharmacokinetic Properties of Hemoglobin-Based Oxygen Carriers","volume":"8","author":"Taguchi","year":"2017","journal-title":"J.\u00a0Funct. Biomater."},{"key":"10.1016\/j.bpj.2024.06.015_bib22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.bjhh.2016.08.004","article-title":"A Phase Ib open label, randomized, safety study of SANGUINATE in patients with sickle cell anemia","volume":"39","author":"Misra","year":"2017","journal-title":"Rev. Bras. Hematol. Hemoter."},{"key":"10.1016\/j.bpj.2024.06.015_bib23","doi-asserted-by":"crossref","first-page":"H2392","DOI":"10.1152\/ajpheart.00225.2005","article-title":"Extreme hemodilution with PEG-hemoglobin vs. PEG-albumin","volume":"289","author":"Cabrales","year":"2005","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.bpj.2024.06.015_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0039111","article-title":"PEG-albumin plasma expansion increases expression of MCP-1 evidencing increased circulatory wall shear stress: an experimental study","volume":"7","author":"Hightower","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bpj.2024.06.015_bib25","doi-asserted-by":"crossref","first-page":"R54","DOI":"10.1186\/cc6874","article-title":"Survival time in severe hemorrhagic shock after perioperative hemodilution is longer with PEG-conjugated human serum albumin than with HES 130\/0.4: a microvascular perspective","volume":"12","author":"Martini","year":"2008","journal-title":"Crit. Care"},{"key":"10.1016\/j.bpj.2024.06.015_bib26","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1097\/SHK.0000000000001370","article-title":"Microvascular and Systemic Impact of Resuscitation with PEGylated Carboxyhemoglobin-Based Oxygen Carrier or Hetastarch in a Rat Model of Transient Hemorrhagic Shock","volume":"53","author":"Nugent","year":"2020","journal-title":"Shock"},{"key":"10.1016\/j.bpj.2024.06.015_bib27","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1152\/japplphysiol.00404.2004","article-title":"Comparison of PEG-modified albumin and hemoglobin in extreme hemodilution in the rat","volume":"97","author":"Winslow","year":"2004","journal-title":"J.\u00a0Appl. Physiol."},{"key":"10.1016\/j.bpj.2024.06.015_bib28","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1053\/j.jvca.2008.08.006","article-title":"Hemospan improves outcome in a model of perioperative hemodilution and blood loss in the rat: comparison with hydroxyethyl starch","volume":"23","author":"Young","year":"2009","journal-title":"J.\u00a0Cardiothorac. Vasc. Anesth."},{"key":"10.1016\/j.bpj.2024.06.015_bib29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1042\/CS20040001","article-title":"Plasma expansion by polyethylene-glycol-modified albumin","volume":"107","author":"Assaly","year":"2004","journal-title":"Clin. Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib30","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1529\/biophysj.107.114314","article-title":"Solution structure of poly(ethylene) glycol-conjugated hemoglobin revealed by small-angle X-ray scattering: implications for a new oxygen therapeutic","volume":"94","author":"Svergun","year":"2008","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib31","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1021\/acs.biochem.5b00246","article-title":"Chemical Interactions of Polyethylene Glycols (PEGs) and Glycerol with Protein Functional Groups: Applications to Effects of PEG and Glycerol on Protein Processes","volume":"54","author":"Knowles","year":"2015","journal-title":"Biochemist"},{"key":"10.1016\/j.bpj.2024.06.015_bib32","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1002\/bip.22662","article-title":"Separating Chemical and Excluded Volume Interactions of Polyethylene Glycols with Native Proteins: Comparison with PEG Effects on DNA Helix Formation","volume":"103","author":"Shkel","year":"2015","journal-title":"Biopolymers"},{"key":"10.1016\/j.bpj.2024.06.015_bib33","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1021\/bc049843w","article-title":"Prediction of the viscosity radius and the size exclusion chromatography behavior of PEGylated proteins","volume":"15","author":"Fee","year":"2004","journal-title":"Bioconjugate Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.bpj.2024.05.019","article-title":"Molecular dynamics simulations of pegylated human serum albumin: Implications for hydrodynamic properties of random coil structure","volume":"123","author":"Fleming","year":"2024","journal-title":"Biophys. J."},{"key":"10.1016\/j.bpj.2024.06.015_bib35","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1006\/abio.1995.1030","article-title":"An optical thermometer for direct measurement of cell temperature in the Beckman instruments XL-A analytical ultracentrifuge","volume":"224","author":"Liu","year":"1995","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bpj.2024.06.015_bib36","series-title":"Analyical Ultracentrifugation in Biochemistry and Polymer Sciences","article-title":"Computer-Aided Interpretation of analytical sedimentation data for proteins","author":"Laue","year":"1992"},{"key":"10.1016\/j.bpj.2024.06.015_bib37","unstructured":"Hayes, D.B., J.P. Philo, T.M. Laue, SEDNTERP: Interpretation of Sedimentation Data P. 2000 Lines of Visual Basic Code, Written for Windows 3."},{"key":"10.1016\/j.bpj.2024.06.015_bib38","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s00249-023-01629-0","article-title":"SEDNTERP: a calculation and database utility to aid interpretation of analytical ultracentrifugation and light scattering data","volume":"52","author":"Philo","year":"2023","journal-title":"Eur. Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib39","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1002\/pol.1978.180160617","article-title":"Volumetric Properties of Dilute Aqueous-Solutions of Poly(Ethylene Glycols)","volume":"16","author":"Lepori","year":"1978","journal-title":"J.\u00a0Polym. Sci. Polym. Phys. Ed"},{"key":"10.1016\/j.bpj.2024.06.015_bib40","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0014-5793(81)81022-8","article-title":"Effect of inert polymers on protein self-association","volume":"126","author":"Nichol","year":"1981","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bpj.2024.06.015_bib41","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/S0006-3495(98)77849-X","article-title":"Hydration of Polyethylene Glycol-Grafted Liposomes","volume":"74","author":"Tirosh","year":"1998","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib42","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0169-409X(02)00022-4","article-title":"Chemistry for peptide and protein PEGylation","volume":"54","author":"Roberts","year":"2002","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.bpj.2024.06.015_bib43","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s00249-022-01627-8","article-title":"Revisiting macromolecular hydration with HullRadSAS","volume":"52","author":"Fleming","year":"2023","journal-title":"Eur. Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib44","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s00249-020-01474-5","article-title":"Analysis of nonideality: insights from high concentration simulations of sedimentation velocity data","volume":"49","author":"Correia","year":"2020","journal-title":"Eur. Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib45","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.bpc.2003.10.027","article-title":"Sedimentation velocity, multi-speed method for analyzing polydisperse solutions","volume":"108","author":"Stafford","year":"2004","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib46","series-title":"Mathematical Theory of Sedimentation Analysis","author":"Fujita","year":"1962"},{"key":"10.1016\/j.bpj.2024.06.015_bib47","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.bpc.2003.10.028","article-title":"Analysis of heterologous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium and kinetic constants","volume":"108","author":"Stafford","year":"2004","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib48","series-title":"Noninteracting Systems by Sedimentation Velocity Analytical Ultracentrifugation. Analytical Ultracentrifugation","first-page":"463","article-title":"Analysis of Nonideal, Interacting","author":"Stafford","year":"2016"},{"key":"10.1016\/j.bpj.2024.06.015_bib49","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ab.2018.04.002","article-title":"Characterization of\u00a0therapeutic antibodies in the presence of human serum proteins by AU-FDS analytical ultracentrifugation","volume":"550","author":"Wright","year":"2018","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bpj.2024.06.015_bib50","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1002\/pro.3422","article-title":"Weak IgG self- and heteroassociation characterized by fluorescence analytical ultracentrifugation","volume":"27","author":"Yang","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib51","first-page":"203","article-title":"Sedimentation coefficients of self-associating species II. Tests with a simulated example and with \u03b2-lactoglobulin A","volume":"17","author":"Beckerdite","year":"1983","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib52","series-title":"SEDANAL: Model-dependent and Model-independent Analysis of Sedimentation Data","first-page":"81","author":"Sherwood","year":"2016"},{"key":"10.1016\/j.bpj.2024.06.015_bib53","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/bs.mie.2015.06.042","article-title":"Sedimentation velocity: a classical perspective","volume":"562","author":"Correia","year":"2015","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bpj.2024.06.015_bib54","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1002\/bip.1976.360150504","article-title":"Sedimentation of generalized systems of interacting particles. III. Concentration-dependent sedimentation and extension to other transport methods","volume":"15","author":"Claverie","year":"1976","journal-title":"Biopolymers"},{"key":"10.1016\/j.bpj.2024.06.015_bib55","doi-asserted-by":"crossref","first-page":"6739","DOI":"10.1073\/pnas.78.11.6739","article-title":"General solution to the inverse problem of the differential equation of the ultracentrifuge","volume":"78","author":"Todd","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2024.06.015_bib56","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0301-4622(83)80017-9","article-title":"Generalized finite element solution to One-Dimensional Flux Problems","volume":"17","author":"Todd","year":"1983","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bpj.2024.06.015_bib57","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S0076-6879(08)04215-8","article-title":"Extracting Equilibrium Constants from Kinetically Limited Reacting Systems","volume":"455","author":"Correia","year":"2009","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bpj.2024.06.015_bib58","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0076-6879(09)67006-3","article-title":"Effect of Kinetics on Sedimentation Velocity Profiles and the Role of Intermediates","volume":"467","author":"Correia","year":"2009","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bpj.2024.06.015_bib59","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ab.2015.08.020","article-title":"The osmotic second virial coefficient for protein self-interaction: Use and misuse to describe thermodynamic nonideality","volume":"490","author":"Wills","year":"2015","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bpj.2024.06.015_bib60","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1749-6632.1969.tb14043.x","article-title":"Studies of Self-Associating Systems by Equilibrium Ultracentrifugation","volume":"164","author":"Roark","year":"1969","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib61","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0006-3495(81)84753-4","article-title":"Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques","volume":"36","author":"Johnson","year":"1981","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib62","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1021\/bi00891a003","article-title":"Equilibrium Ultracentrifugation of Dilute Solutions","volume":"3","author":"Yphantis","year":"1964","journal-title":"Biochemistry"},{"key":"10.1016\/j.bpj.2024.06.015_bib80","doi-asserted-by":"crossref","first-page":"eabb0372","DOI":"10.1126\/sciadv.abb0372","article-title":"A single molecular descriptor to predict solution behavior of therapeutic antibodies","volume":"6","author":"Kingsbury","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.bpj.2024.06.015_bib63","series-title":"Physical Chemistry of Macromolecules","author":"Tanford","year":"1961"},{"key":"10.1016\/j.bpj.2024.06.015_bib65","series-title":"Real-time Electrophoretic Mobility in Membrane Confined Electrophoresis","author":"Jordan","year":"2014"},{"key":"10.1016\/j.bpj.2024.06.015_bib64","first-page":"865","article-title":"HullRad: Fast Calculations of Folded and Disordered Protein and Nucleic Acid Hydrodynamic Properties","volume":"27","author":"Fleming","year":"2018","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib66","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1002\/bip.1977.360161202","article-title":"The concentration dependence of transport processes: A general description applicable to the sedimentation, translational diffusion, and viscosity coefficients of macromolecular solutes","volume":"16","author":"Rowe","year":"1977","journal-title":"Biopolymers"},{"key":"10.1016\/j.bpj.2024.06.015_bib67","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0926-6585(65)90145-7","article-title":"On the estimation of the shape of macromolecules from sedimentation and viscosity measurements","volume":"102","author":"Creeth","year":"1965","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bpj.2024.06.015_bib68","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1042\/bj2310549","article-title":"Physicochemical studies on turnip-yellow-mosaic virus. Homogeneity, relative molecular masses, hydrodynamic radii and concentration-dependence of parameters in non-dissociating solvents","volume":"231","author":"Harding","year":"1985","journal-title":"Biochem.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib69","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1042\/bj2310543","article-title":"The concentration-dependence of macromolecular parameters","volume":"231","author":"Harding","year":"1985","journal-title":"Biochem.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib70","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1016\/S0006-3495(01)75838-9","article-title":"Non-ideality by sedimentation velocity of halophilic malate dehydrogenase in complex solvents","volume":"81","author":"Solovyova","year":"2001","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib71","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1016\/j.bpj.2010.08.020","article-title":"Diffusion and sedimentation interaction parameters for measuring the second virial coefficient and their utility as predictors of protein aggregation","volume":"99","author":"Saluja","year":"2010","journal-title":"Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib72","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ab.2010.12.014","article-title":"Use of dynamic light scattering to determine second virial coefficient in a semidilute concentration regime","volume":"411","author":"Yadav","year":"2011","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bpj.2024.06.015_bib73","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1002\/jps.22831","article-title":"Viscosity behavior of high-concentration monoclonal antibody solutions: correlation with interaction parameter and electroviscous effects","volume":"101","author":"Yadav","year":"2012","journal-title":"J.\u00a0Pharmaceut. Sci."},{"key":"10.1016\/j.bpj.2024.06.015_bib74","doi-asserted-by":"crossref","DOI":"10.1080\/19420862.2020.1810488","article-title":"Measuring aggregates, self-association, and weak interactions in concentrated therapeutic antibody solutions","volume":"12","author":"Chaturvedi","year":"2020","journal-title":"mAbs"},{"key":"10.1016\/j.bpj.2024.06.015_bib75","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.1038\/s41598-021-84946-8","article-title":"Global multi-method analysis of interaction parameters for reversibly self-associating macromolecules at high concentrations","volume":"11","author":"Parupudi","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bpj.2024.06.015_bib76","doi-asserted-by":"crossref","first-page":"2995","DOI":"10.1063\/1.1732415","article-title":"Concentration Dependence of Frictional Coefficient of Polymers in Solution","volume":"36","author":"Yamakawa","year":"1962","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.bpj.2024.06.015_bib77","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1021\/ma60057a023","article-title":"Diffusion of Polystyrene in a Theta Mixed Solvent (Benzene-2-Propanol) by Photon-Correlation Spectroscopy","volume":"10","author":"Frost","year":"1977","journal-title":"Macromolecules"},{"key":"10.1016\/j.bpj.2024.06.015_bib78","series-title":"Fluid Systems","article-title":"Nonequilibrium Thermodynamic: A Phenomenological Theory of Irreversible Processes","author":"Fitts","year":"1962"},{"key":"10.1016\/j.bpj.2024.06.015_bib79","doi-asserted-by":"crossref","first-page":"6242","DOI":"10.1039\/C4AN01060D","article-title":"Concentration dependence of translational diffusion coefficients for globular proteins","volume":"139","author":"Scott","year":"2014","journal-title":"Analyst"},{"key":"10.1016\/j.bpj.2024.06.015_bib81","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s00249-023-01652-1","article-title":"Sedimentation Velocity FDS Studies of Antibodies in Pooled Human Serum","volume":"52","author":"Correia","year":"2023","journal-title":"Eur. Biophys.\u00a0J."},{"key":"10.1016\/j.bpj.2024.06.015_bib82","doi-asserted-by":"crossref","first-page":"5837","DOI":"10.1021\/acs.jpcb.3c02356","article-title":"Excluded Volume and Weak Interactions in Crowded Solutions Modulate Conformations and RNA Binding of an Intrinsically Disordered Tail","volume":"127","author":"Stringer","year":"2023","journal-title":"J.\u00a0Phys. Chem. B"},{"key":"10.1016\/j.bpj.2024.06.015_bib83","doi-asserted-by":"crossref","first-page":"4874","DOI":"10.1073\/pnas.1322611111","article-title":"Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments","volume":"111","author":"Soranno","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2024.06.015_bib84","first-page":"4874","article-title":"Depletion interactions modulate the binding between disordered proteins in crowded environments","volume":"111","author":"Zosela","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bpj.2024.06.015_bib85","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0301-4622(01)00213-7","article-title":"The hydration problem in solution biophysics: an introduction","volume":"93","author":"Harding","year":"2001","journal-title":"Biophys. Chem."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349524004120?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349524004120?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:46:36Z","timestamp":1762101996000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349524004120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":85,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["S0006349524004120"],"URL":"https:\/\/doi.org\/10.1016\/j.bpj.2024.06.015","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Hydrodynamic and thermodynamic analysis of\u00a0PEGylated human serum albumin","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2024.06.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.bpj.2024.05.019","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Biophysical Society.","name":"copyright","label":"Copyright"}]}}