{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T11:27:15Z","timestamp":1774870035255,"version":"3.50.1"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"CounterACT Program","award":["UO1NS058213"],"award-info":[{"award-number":["UO1NS058213"]}]},{"name":"National Institutes of Health Office of the Director","award":["RO1NS051505"],"award-info":[{"award-number":["RO1NS051505"]}]},{"name":"National Institutes of Health Office of the Director","award":["RO1NS052529"],"award-info":[{"award-number":["RO1NS052529"]}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Research"],"published-print":{"date-parts":[[2011,1]]},"DOI":"10.1016\/j.brainres.2010.11.065","type":"journal-article","created":{"date-parts":[[2010,11,26]],"date-time":"2010-11-26T09:12:48Z","timestamp":1290762768000},"page":"110-120","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["An organotypic hippocampal slice culture model of excitotoxic injury induced spontaneous recurrent epileptiform discharges"],"prefix":"10.1016","volume":"1371","author":[{"given":"Julie M.","family":"Ziobro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laxmikant S.","family":"Deshpande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"DeLorenzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.brainres.2010.11.065_bb0005","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/bjp.2008.112","article-title":"Standard antiepileptic drugs fail to block epileptiform activity in rat organotypic hippocampal slice cultures","volume":"154","author":"Albus","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0010","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/0006-8993(90)91771-8","article-title":"Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in mg-free and physiological medium","volume":"532","author":"Anderson","year":"1990","journal-title":"Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0015","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0006-8993(86)91274-6","article-title":"Magnesium-free medium activates seizure-like events in the rat hippocampal slice","volume":"398","author":"Anderson","year":"1986","journal-title":"Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0020","doi-asserted-by":"crossref","first-page":"570","DOI":"10.4065\/71.6.570","article-title":"Causes of epilepsy: contributions of the Rochester Epidemiology Project","volume":"71","author":"Annegers","year":"1996","journal-title":"Mayo Clin. Proc."},{"key":"10.1016\/j.brainres.2010.11.065_bb0025","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1111\/j.1528-1167.2009.02173.x","article-title":"Pursuing paradoxical proconvulsant prophylaxis for epileptogenesis","volume":"50","author":"Armstrong","year":"2009","journal-title":"Epilepsia"},{"key":"10.1016\/j.brainres.2010.11.065_bb0030","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1002\/hipo.450050505","article-title":"Stable maintenance of glutamate receptors and other synaptic components in long-term hippocampal slices","volume":"5","author":"Bahr","year":"1995","journal-title":"Hippocampus"},{"key":"10.1016\/j.brainres.2010.11.065_bb0035","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1152\/jn.01028.2003","article-title":"Contributions of mossy fiber and CA1 pyramidal cell sprouting to dentate granule cell hyperexcitability in kainic acid-treated hippocampal slice cultures","volume":"92","author":"Bausch","year":"2004","journal-title":"J. Neurophysiol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0040","doi-asserted-by":"crossref","first-page":"2918","DOI":"10.1152\/jn.2000.84.6.2918","article-title":"Synaptic connections from multiple subfields contribute to granule cell hyperexcitability in hippocampal slice cultures","volume":"84","author":"Bausch","year":"2000","journal-title":"J. Neurophysiol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0045","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1152\/jn.00355.2006","article-title":"Plasticity of both excitatory and inhibitory synapses is associated with seizures induced by removal of chronic blockade of activity in cultured hippocampus","volume":"96","author":"Bausch","year":"2006","journal-title":"J. Neurophysiol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0050","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.neuropharm.2006.03.024","article-title":"Retigabine and flupirtine exert neuroprotective actions in organotypic hippocampal cultures","volume":"51","author":"Boscia","year":"2006","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.brainres.2010.11.065_bb0055","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0022-510X(98)00034-3","article-title":"Epileptiform discharges to extracellular stimuli in rat neocortical slices after photothrombotic infarction","volume":"156","author":"Buchkremer-Ratzmann","year":"1998","journal-title":"J. Neurol. Sci."},{"key":"10.1016\/j.brainres.2010.11.065_bb0060","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1161\/01.STR.26.11.2187","article-title":"Massive persistent release of excitatory amino acids following human occlusive stroke","volume":"26","author":"Bullock","year":"1995","journal-title":"Stroke"},{"key":"10.1016\/j.brainres.2010.11.065_bb0065","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2174\/157015907780077105","article-title":"Brain slices as models for neurodegenerative disease and screening platforms to identify novel therapeutics","volume":"5","author":"Cho","year":"2007","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0070","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1006\/nbdi.2000.0354","article-title":"Stroke","volume":"7","author":"Choi","year":"2000","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.brainres.2010.11.065_bb0075","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/0006-8993(90)91279-P","article-title":"NMDA receptor blockade inhibits glutamate-induced kindling of the rat amygdala","volume":"506","author":"Croucher","year":"1990","journal-title":"Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0080","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0014-2999(88)90832-1","article-title":"Inhibition of the development of electrical kindling of the prepyriform cortex by daily focal injections of excitatory amino acid antagonists","volume":"152","author":"Croucher","year":"1988","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0085","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1161\/01.STR.28.4.708","article-title":"Duration of glutamate release after acute ischemic stroke","volume":"28","author":"Davalos","year":"1997","journal-title":"Stroke"},{"key":"10.1016\/j.brainres.2010.11.065_bb0090","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0074-7742(06)81005-6","article-title":"An in vitro model of stroke-induced epilepsy: elucidation of the roles of glutamate and calcium in the induction and maintenance of stroke-induced epileptogenesis","volume":"81","author":"DeLorenzo","year":"2007","journal-title":"Int. Rev. Neurobiol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0095","doi-asserted-by":"crossref","first-page":"14482","DOI":"10.1073\/pnas.95.24.14482","article-title":"Prolonged activation of the N-methyl-d-aspartate receptor-Ca2+ transduction pathway causes spontaneous recurrent epileptiform discharges in hippocampal neurons in culture","volume":"95","author":"DeLorenzo","year":"1998","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.brainres.2010.11.065_bb0100","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1126\/science.3037700","article-title":"Cellular mechanisms of epilepsy: a status report","volume":"237","author":"Dichter","year":"1987","journal-title":"Science"},{"key":"10.1016\/j.brainres.2010.11.065_bb0105","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/S0166-2236(99)01401-0","article-title":"Pathobiology of ischaemic stroke: an integrated view","volume":"22","author":"Dirnagl","year":"1999","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.brainres.2010.11.065_bb0110","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1006\/cbir.1999.0397","article-title":"\u201cNon-synaptic\u201d mechanisms in seizures and epileptogenesis","volume":"22","author":"Dudek","year":"1998","journal-title":"Cell Biol. Int."},{"key":"10.1016\/j.brainres.2010.11.065_bb0115","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1528-1157.1991.tb04675.x","article-title":"Prevalence of epilepsy in Rochester, Minnesota: 1940\u20131980","volume":"32","author":"Hauser","year":"1991","journal-title":"Epilepsia"},{"key":"10.1016\/j.brainres.2010.11.065_bb0120","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1212\/WNL.59.9_suppl_5.S21","article-title":"Epilepsy after brain insult: targeting epileptogenesis","volume":"59","author":"Herman","year":"2002","journal-title":"Neurology"},{"key":"10.1016\/j.brainres.2010.11.065_bb0125","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1046\/j.1471-4159.2000.0751590.x","article-title":"Synaptic activity-dependent developmental regulation of NMDA receptor subunit expression in cultured neocortical neurons","volume":"75","author":"Hoffmann","year":"2000","journal-title":"J. Neurochem."},{"key":"10.1016\/j.brainres.2010.11.065_bb0130","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1007\/s11064-005-8831-y","article-title":"Epileptogenesis after experimental focal cerebral ischemia","volume":"30","author":"Karhunen","year":"2005","journal-title":"Neurochem. Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0135","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.eplepsyres.2006.07.003","article-title":"A long-term video-EEG and behavioral follow-up after endothelin-1 induced middle cerebral artery occlusion in rats","volume":"72","author":"Karhunen","year":"2006","journal-title":"Epilepsy Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0140","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0306-4522(01)00168-3","article-title":"Post-insult activity is a major cause of delayed neuronal death in organotypic hippocampal slices exposed to glutamate","volume":"105","author":"Lahtinen","year":"2001","journal-title":"Neuroscience"},{"key":"10.1016\/j.brainres.2010.11.065_bb0145","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.neuroscience.2007.02.003","article-title":"Neuroprotective potential of ceftriaxone in in vitro models of stroke","volume":"146","author":"Lipski","year":"2007","journal-title":"Neuroscience"},{"issue":"Suppl 2","key":"10.1016\/j.brainres.2010.11.065_bb0150","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1111\/j.1528-1157.1995.tb05996.x","article-title":"Antiepileptic drug mechanisms of action","volume":"36","author":"Macdonald","year":"1995","journal-title":"Epilepsia"},{"key":"10.1016\/j.brainres.2010.11.065_bb0155","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0165-0270(89)90051-4","article-title":"Rat hippocampal slices \u201cin vitro\u201d display spontaneous epileptiform activity following long-term organotypic culture","volume":"27","author":"McBain","year":"1989","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.brainres.2010.11.065_bb0160","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1523\/JNEUROSCI.14-06-03413.1994","article-title":"Cellular and molecular basis of epilepsy","volume":"14","author":"McNamara","year":"1994","journal-title":"J. Neurosci."},{"key":"10.1016\/j.brainres.2010.11.065_bb0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.eplepsyres.2009.08.007","article-title":"Ischaemic stroke in adults and epilepsy","volume":"87","author":"Menon","year":"2009","journal-title":"Epilepsy Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0170","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S0169-328X(00)00112-1","article-title":"Chronic DeltaFosB expression and increased AP-1 transcription factor binding are associated with the long term plasticity changes in epilepsy","volume":"79","author":"Morris","year":"2000","journal-title":"Brain Res. Mol. Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0175","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0165-3806(93)90109-N","article-title":"Time course of synaptic development in hippocampal organotypic cultures","volume":"71","author":"Muller","year":"1993","journal-title":"Brain Res. Dev. Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0180","doi-asserted-by":"crossref","first-page":"435","DOI":"10.2174\/1568007054546108","article-title":"Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair","volume":"4","author":"Noraberg","year":"2005","journal-title":"Curr. Drug Targets CNS Neurol. Disord."},{"key":"10.1016\/j.brainres.2010.11.065_bb0185","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0165-3806(00)00132-2","article-title":"High oxygen tension leads to acute cell death in organotypic hippocampal slice cultures","volume":"126","author":"Pomper","year":"2001","journal-title":"Dev. Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0190","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/S0006-8993(97)01285-7","article-title":"NMDA receptor activation during status epilepticus is required for the development of epilepsy","volume":"782","author":"Rice","year":"1998","journal-title":"Brain Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0195","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1177\/026119291003800108","article-title":"The use of organotypic hippocampal slice cultures to evaluate protection by non-competitive NMDA receptor antagonists against excitotoxicity","volume":"38","author":"Ring","year":"2010","journal-title":"Altern. Lab. Anim."},{"key":"10.1016\/j.brainres.2010.11.065_bb0200","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0031-6997(25)00046-8","article-title":"Antiepileptic drugs: pharmacological mechanisms and clinical efficacy with consideration of promising developmental stage compounds","volume":"42","author":"Rogawski","year":"1990","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.brainres.2010.11.065_bb0205","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S0306-4522(99)00358-9","article-title":"Seizures, cell death, and mossy fiber sprouting in kainic acid-treated organotypic hippocampal cultures","volume":"94","author":"Routbort","year":"1999","journal-title":"Neuroscience"},{"key":"10.1016\/j.brainres.2010.11.065_bb0210","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.neuroscience.2008.05.052","article-title":"Heterologous expression of the invertebrate FMRFamide-gated sodium channel as a mechanism to selectively activate mammalian neurons","volume":"155","author":"Schanuel","year":"2008","journal-title":"Neuroscience"},{"key":"10.1016\/j.brainres.2010.11.065_bb0215","series-title":"Neurochemistry and Molecular Biology","first-page":"51","article-title":"Stroke: pathophysiology, diagnosis, and management","author":"Sharp","year":"1998"},{"key":"10.1016\/j.brainres.2010.11.065_bb0220","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1152\/jn.1995.73.4.1706","article-title":"Recurrent spontaneous seizure activity in hippocampal neuronal networks in culture","volume":"73","author":"Sombati","year":"1995","journal-title":"J. Neurophysiol."},{"key":"10.1016\/j.brainres.2010.11.065_bb0225","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1126\/science.2569762","article-title":"NMDA antagonists differentiate epileptogenesis from seizure expression in an in vitro model","volume":"245","author":"Stasheff","year":"1989","journal-title":"Science"},{"key":"10.1016\/j.brainres.2010.11.065_bb0230","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0165-0270(91)90128-M","article-title":"A simple method for organotypic cultures of nervous tissue","volume":"37","author":"Stoppini","year":"1991","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.brainres.2010.11.065_bb0235","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1161\/hs1001.097242","article-title":"Glutamate injury-induced epileptogenesis in hippocampal neurons: an in vitro model of stroke-induced \u201cepilepsy\u201d","volume":"32","author":"Sun","year":"2001","journal-title":"Stroke"},{"key":"10.1016\/j.brainres.2010.11.065_bb0240","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ceca.2003.09.003","article-title":"Long-lasting alterations in neuronal calcium homeostasis in an in vitro model of stroke-induced epilepsy","volume":"35","author":"Sun","year":"2004","journal-title":"Cell Calcium"},{"key":"10.1016\/j.brainres.2010.11.065_bb0245","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/S1359-6446(05)03502-6","article-title":"Organotypic cultures as tools for functional screening in the CNS","volume":"10","author":"Sundstrom","year":"2005","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.brainres.2010.11.065_bb0250","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1161\/01.STR.27.9.1459","article-title":"Lifetime cost of stroke in the United States","volume":"27","author":"Taylor","year":"1996","journal-title":"Stroke"},{"key":"10.1016\/j.brainres.2010.11.065_bb0255","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1212\/WNL.39.5.676","article-title":"GABAergic neocortical neurons are resistant to NMDA receptor-mediated injury","volume":"39","author":"Tecoma","year":"1989","journal-title":"Neurology"},{"key":"10.1016\/j.brainres.2010.11.065_bb0260","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.neuroscience.2004.11.002","article-title":"Sprouting of mossy fibers and presynaptic inhibition by group II metabotropic glutamate receptors in pilocarpine-treated rat hippocampal slice cultures","volume":"131","author":"Thomas","year":"2005","journal-title":"Neuroscience"},{"key":"10.1016\/j.brainres.2010.11.065_bb0265","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.eplepsyres.2010.06.007","article-title":"Drug refractoriness of epileptiform activity in organotypic hippocampal slice cultures depends on the mode of provocation","volume":"90","author":"Wahab","year":"2010","journal-title":"Epilepsy Res."},{"key":"10.1016\/j.brainres.2010.11.065_bb0270","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.neuropharm.2008.07.046","article-title":"Regulation of NMDA receptor subunit expression and its implications for LTD, LTP, and metaplasticity","volume":"55","author":"Yashiro","year":"2008","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.brainres.2010.11.065_bb0275","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1002\/cne.902280310","article-title":"Cellular and connective organization of slice cultures of the rat hippocampus and fascia dentata","volume":"228","author":"Zimmer","year":"1984","journal-title":"J. Comp. Neurol."}],"container-title":["Brain Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006899310025783?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006899310025783?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T06:44:22Z","timestamp":1740725062000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006899310025783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":55,"alternative-id":["S0006899310025783"],"URL":"https:\/\/doi.org\/10.1016\/j.brainres.2010.11.065","relation":{},"ISSN":["0006-8993"],"issn-type":[{"value":"0006-8993","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1]]}}}