{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T12:12:05Z","timestamp":1768997525802,"version":"3.49.0"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Brain Research"],"published-print":{"date-parts":[[2017,11]]},"DOI":"10.1016\/j.brainres.2017.08.033","type":"journal-article","created":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T01:00:16Z","timestamp":1504314016000},"page":"20-27","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Prolonged but non-permanent expression of a transgene in ependymal cells of adult rats using an adenovirus-mediated transposon gene transfer system"],"prefix":"10.1016","volume":"1675","author":[{"given":"Jun-ichi","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Dezawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4103-9767","authenticated-orcid":false,"given":"Masaaki","family":"Kitada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.brainres.2017.08.033_b0005","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/ng0393-229","article-title":"Direct in vivo gene transfer to ependymal cells in the central nervous system using recombinant adenovirus vectors","volume":"3","author":"Bajocchi","year":"1993","journal-title":"Nat. Genet."},{"key":"10.1016\/j.brainres.2017.08.033_b0010","doi-asserted-by":"crossref","first-page":"e169","DOI":"10.1371\/journal.pgen.0020169","article-title":"Harnessing a high cargo-capacity transposon for genetic applications in vertebrates","volume":"2","author":"Balciunas","year":"2006","journal-title":"PLoS Genet."},{"key":"10.1016\/j.brainres.2017.08.033_b0015","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.stem.2010.07.014","article-title":"Origin of new glial cells in intact and injured adult spinal cord","volume":"7","author":"Barnabe-Heider","year":"2010","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.brainres.2017.08.033_b0020","doi-asserted-by":"crossref","first-page":"6718","DOI":"10.1523\/JNEUROSCI.21-17-06718.2001","article-title":"Adenoviral brain-derived neurotrophic factor induces both neostriatal and olfactory neuronal recruitment from endogenous progenitor cells in the adult forebrain","volume":"21","author":"Benraiss","year":"2001","journal-title":"J. Neurosci."},{"key":"10.1016\/j.brainres.2017.08.033_b0025","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/gt.2011.114","article-title":"Sustained induction of neuronal addition to the adult rat neostriatum by AAV4-delivered noggin and BDNF","volume":"19","author":"Benraiss","year":"2012","journal-title":"Gene Ther."},{"key":"10.1016\/j.brainres.2017.08.033_b0030","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/2045-8118-11-10","article-title":"A new look at cerebrospinal fluid circulation","volume":"11","author":"Brinker","year":"2014","journal-title":"Fluids Barriers CNS"},{"key":"10.1016\/j.brainres.2017.08.033_b0035","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nn.2268","article-title":"Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke","volume":"12","author":"Carlen","year":"2009","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.brainres.2017.08.033_b0040","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1523\/JNEUROSCI.19-11-04462.1999","article-title":"Adult mammalian forebrain ependymal and subependymal cells demonstrate proliferative potential, but only subependymal cells have neural stem cell characteristics","volume":"19","author":"Chiasson","year":"1999","journal-title":"J. Neurosci."},{"key":"10.1016\/j.brainres.2017.08.033_b0045","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1172\/JCI31778","article-title":"Induction of neostriatal neurogenesis slows disease progression in a transgenic murine model of Huntington disease","volume":"117","author":"Cho","year":"2007","journal-title":"J Clin Invest."},{"key":"10.1016\/j.brainres.2017.08.033_b0050","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1161\/01.STR.29.7.1411","article-title":"Adenovirus-mediated gene transfer in vivo to cerebral blood vessels and perivascular tissue in mice","volume":"29","author":"Christenson","year":"1998","journal-title":"Stroke"},{"key":"10.1016\/j.brainres.2017.08.033_b0055","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.2174\/138161212799859611","article-title":"Neural stem cell niches in health and diseases","volume":"18","author":"Decimo","year":"2012","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.brainres.2017.08.033_b0060","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.gde.2003.08.012","article-title":"A niche for adult neural stem cells","volume":"13","author":"Doetsch","year":"2003","journal-title":"Curr. Opin. Genet. Dev."},{"key":"10.1016\/j.brainres.2017.08.033_b0065","first-page":"962","article-title":"Design and optimization of lentiviral vectors for transfer of GALC expression in Twitcher brain","volume":"8","author":"Dolcetta","year":"2006","journal-title":"J. Genet. Med."},{"key":"10.1016\/j.brainres.2017.08.033_b0070","doi-asserted-by":"crossref","first-page":"8824","DOI":"10.1128\/MCB.00498-07","article-title":"Postintegrative gene silencing within the Sleeping Beauty transposition system","volume":"27","author":"Garrison","year":"2007","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.brainres.2017.08.033_b0075","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jconrel.2012.04.008","article-title":"Virus-mediated gene delivery for human gene therapy","volume":"161","author":"Giacca","year":"2012","journal-title":"J. Control. Release"},{"key":"10.1016\/j.brainres.2017.08.033_b0080","doi-asserted-by":"crossref","first-page":"6855","DOI":"10.1073\/pnas.1411263112","article-title":"Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain","volume":"112","author":"Goff","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.brainres.2017.08.033_b0085","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/mt.2010.47","article-title":"Comparative analysis of transposable element vector systems in human cells","volume":"18","author":"Grabundzija","year":"2010","journal-title":"Mol. Ther."},{"key":"10.1016\/j.brainres.2017.08.033_b0090","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/dvg.20234","article-title":"Tol2 transposon-mediated transgenesis in Xenopus tropicalis","volume":"44","author":"Hamlet","year":"2006","journal-title":"Genesis"},{"key":"10.1016\/j.brainres.2017.08.033_b0095","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2174\/156652311797415890","article-title":"Development of adenovirus hybrid vectors for Sleeping Beauty transposition in large mammals","volume":"11","author":"Hausl","year":"2011","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.brainres.2017.08.033_b0100","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0092-8674(00)80956-3","article-title":"Identification of a neural stem cell in the adult mammalian central nervous system","volume":"96","author":"Johansson","year":"1999","journal-title":"Cell"},{"key":"10.1016\/j.brainres.2017.08.033_b0105","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aac6191","article-title":"AAV gene transfer delays disease onset in a TPP1-deficient canine model of the late infantile form of Batten disease","volume":"7","author":"Katz","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.brainres.2017.08.033_b0110","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0378-1119(99)00444-8","article-title":"Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excision of a nonautonomous Tol2 element in zebrafish Danio rerio","volume":"240","author":"Kawakami","year":"1999","journal-title":"Gene"},{"key":"10.1016\/j.brainres.2017.08.033_b0115","doi-asserted-by":"crossref","first-page":"11403","DOI":"10.1073\/pnas.97.21.11403","article-title":"Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage","volume":"97","author":"Kawakami","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.brainres.2017.08.033_b0120","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1534\/genetics.166.2.895","article-title":"Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells","volume":"166","author":"Kawakami","year":"2004","journal-title":"Genetics"},{"key":"10.1016\/j.brainres.2017.08.033_b0125","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.devcel.2004.06.005","article-title":"A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish","volume":"7","author":"Kawakami","year":"2004","journal-title":"Dev. Cell"},{"key":"10.1016\/j.brainres.2017.08.033_b0130","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00418-016-1421-z","article-title":"Regulation of DM-20 mRNA expression and intracellular translocation of glutathione-S-transferase pi isoform during oligodendrocyte differentiation in the adult rat spinal cord","volume":"146","author":"Kitada","year":"2016","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.brainres.2017.08.033_b0135","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1261\/rna.2565011","article-title":"Identification of piRNAs in the central nervous system","volume":"17","author":"Lee","year":"2011","journal-title":"RNA"},{"key":"10.1016\/j.brainres.2017.08.033_b0140","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1634\/stemcells.21-1-111","article-title":"High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors","volume":"21","author":"Ma","year":"2003","journal-title":"Stem Cells."},{"key":"10.1016\/j.brainres.2017.08.033_b0145","doi-asserted-by":"crossref","first-page":"E1466","DOI":"10.1073\/pnas.1118715109","article-title":"Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons","volume":"109","author":"Macdonald","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.brainres.2017.08.033_b0150","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1046\/j.1460-9568.2002.02181.x","article-title":"In vivo infusions of exogenous growth factors into the fourth ventricle of the adult mouse brain increase the proliferation of neural progenitors around the fourth ventricle and the central canal of the spinal cord","volume":"16","author":"Martens","year":"2002","journal-title":"Eur. J. Neurosci."},{"key":"10.1016\/j.brainres.2017.08.033_b0155","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S1471-4906(01)01990-1","article-title":"The ependymal route to the CNS: an emerging gene-therapy approach for MS","volume":"22","author":"Martino","year":"2001","journal-title":"Trends Immunol."},{"key":"10.1016\/j.brainres.2017.08.033_b0160","doi-asserted-by":"crossref","first-page":"e187","DOI":"10.1038\/mtna.2014.39","article-title":"Lentivector integration sites in ependymal cells from a model of metachromatic leukodystrophy: non-B DNA as a new factor influencing integration","volume":"3","author":"McAllister","year":"2014","journal-title":"Mol. Ther. Nucl. Acids."},{"key":"10.1016\/j.brainres.2017.08.033_b0165","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1472-6750-11-28","article-title":"Genome-wide target profiling of piggyBac and Tol2 in HEK 293: pros and cons for gene discovery and gene therapy","volume":"11","author":"Meir","year":"2011","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.brainres.2017.08.033_b0170","doi-asserted-by":"crossref","first-page":"e182","DOI":"10.1371\/journal.pbio.0060182","article-title":"Spinal cord injury reveals multilineage differentiation of ependymal cells","volume":"6","author":"Meletis","year":"2008","journal-title":"PLoS Biol."},{"key":"10.1016\/j.brainres.2017.08.033_b0175","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0378-1119(89)90209-6","article-title":"Expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5","volume":"79","author":"Miyazaki","year":"1989","journal-title":"Gene"},{"key":"10.1016\/j.brainres.2017.08.033_b0180","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.neuroscience.2004.10.011","article-title":"Proliferation, migration, and differentiation of endogenous ependymal region stem\/progenitor cells following minimal spinal cord injury in the adult rat","volume":"131","author":"Mothe","year":"2005","journal-title":"Neuroscience"},{"key":"10.1016\/j.brainres.2017.08.033_b0185","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S1525-0016(03)00221-1","article-title":"Targeting expression of a transgene to the airway surface epithelium using a ciliated cell-specific promoter","volume":"8","author":"Ostrowski","year":"2003","journal-title":"Mol. Ther."},{"key":"10.1016\/j.brainres.2017.08.033_b0190","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ygeno.2006.04.007","article-title":"Sleeping Beauty transposition in the mouse genome is associated with changes in DNA methylation at the site of insertion","volume":"88","author":"Park","year":"2006","journal-title":"Genomics"},{"key":"10.1016\/j.brainres.2017.08.033_b0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11060-005-9055-3","article-title":"Cell type- and region-dependent coxsackie adenovirus receptor expression in the central nervous system","volume":"78","author":"Persson","year":"2006","journal-title":"J. Neurooncol."},{"key":"10.1016\/j.brainres.2017.08.033_b0200","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0163-7258(98)00020-5","article-title":"Viral vectors for gene therapy","volume":"80","author":"Robbins","year":"1998","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.brainres.2017.08.033_b0205","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.anorl.2011.03.002","article-title":"Anatomy and physiology of cerebrospinal fluid","volume":"128","author":"Sakka","year":"2011","journal-title":"Eur. Ann. Otorhinolaryngol. Head Neck Dis."},{"key":"10.1016\/j.brainres.2017.08.033_b0210","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.ydbio.2007.01.043","article-title":"Stable integration and conditional expression of electroporated transgenes in chicken embryos","volume":"305","author":"Sato","year":"2007","journal-title":"Dev. Biol."},{"key":"10.1016\/j.brainres.2017.08.033_b0215","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1126\/science.1119133","article-title":"New neurons follow the flow of cerebrospinal fluid in the adult brain","volume":"311","author":"Sawamoto","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.brainres.2017.08.033_b0220","doi-asserted-by":"crossref","first-page":"20131189","DOI":"10.1098\/rsif.2013.1189","article-title":"Flow induced by ependymal cilia dominates near-wall cerebrospinal fluid dynamics in the lateral ventricles","volume":"11","author":"Siyahhan","year":"2014","journal-title":"J.R. Soc. Interface"},{"key":"10.1016\/j.brainres.2017.08.033_b0225","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00418-015-1384-5","article-title":"The expression of PLP\/DM-20 mRNA is restricted to the oligodendrocyte-lineage cells in the adult rat spinal cord","volume":"145","author":"Takeda","year":"2016","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.brainres.2017.08.033_b0230","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0168-0102(00)00176-0","article-title":"Neurons in Golgi-stain-like images revealed by GFP-adenovirus infection in vivo","volume":"38","author":"Tamamaki","year":"2000","journal-title":"Neurosci. Res."},{"key":"10.1016\/j.brainres.2017.08.033_b0235","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1534\/genetics.106.060244","article-title":"Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition","volume":"174","author":"Urasaki","year":"2006","journal-title":"Genetics"},{"key":"10.1016\/j.brainres.2017.08.033_b0240","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1002\/jnr.10346","article-title":"Immunohistochemical and electron microscopic study of invasion and differentiation in spinal cord lesion of neural stem cells grafted through cerebrospinal fluid in rat","volume":"69","author":"Wu","year":"2002","journal-title":"J. Neurosci. Res."},{"key":"10.1016\/j.brainres.2017.08.033_b0245","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.neulet.2005.12.051","article-title":"Increase in bFGF-responsive neural progenitor population following contusion injury of the adult rodent spinal cord","volume":"397","author":"Xu","year":"2006","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.brainres.2017.08.033_b0250","doi-asserted-by":"crossref","first-page":"9814","DOI":"10.1523\/JNEUROSCI.21-24-09814.2001","article-title":"Transcription factor expression and Notch-dependent regulation of neural progenitors in the adult rat spinal cord","volume":"21","author":"Yamamoto","year":"2001","journal-title":"J. Neurosci."},{"key":"10.1016\/j.brainres.2017.08.033_b0255","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1006\/exnr.2001.7798","article-title":"Proliferation of parenchymal neural progenitors in response to injury in the adult rat spinal cord","volume":"172","author":"Yamamoto","year":"2001","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.brainres.2017.08.033_b0260","doi-asserted-by":"crossref","first-page":"5506","DOI":"10.1038\/srep05506","article-title":"Targeted gene transfer into ependymal cells through intraventricular injection of AAV1 vector and long-term enzyme replacement via the CSF","volume":"4","author":"Yamazaki","year":"2014","journal-title":"Sci. Rep."},{"key":"10.1016\/j.brainres.2017.08.033_b0265","doi-asserted-by":"crossref","first-page":"13354","DOI":"10.1073\/pnas.1510194112","article-title":"NT3-chitosan elicits robust endogenous neurogenesis to enable functional recovery after spinal cord injury","volume":"112","author":"Yang","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.brainres.2017.08.033_b0270","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1038\/nbt738","article-title":"Transposition from a gutless adeno-transposon vector stabilizes transgene expression in vivo","volume":"20","author":"Yant","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.brainres.2017.08.033_b0275","doi-asserted-by":"crossref","first-page":"43613","DOI":"10.1038\/srep43613","article-title":"Chromatin states shape insertion profiles of the piggyBac, Tol2 and sleeping beauty transposons and murine leukemia virus","volume":"7","author":"Yoshida","year":"2017","journal-title":"Sci. Rep."}],"container-title":["Brain Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006899317303773?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006899317303773?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T22:31:35Z","timestamp":1570055495000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006899317303773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":55,"alternative-id":["S0006899317303773"],"URL":"https:\/\/doi.org\/10.1016\/j.brainres.2017.08.033","relation":{},"ISSN":["0006-8993"],"issn-type":[{"value":"0006-8993","type":"print"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Prolonged but non-permanent expression of a transgene in ependymal cells of adult rats using an adenovirus-mediated transposon gene transfer system","name":"articletitle","label":"Article Title"},{"value":"Brain Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.brainres.2017.08.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}