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Interfaces"},{"key":"10.1016\/j.bsheal.2024.09.005_b0415","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115929","article-title":"Universal smartphone-assisted label-free CRISPR \/ Cas12a-dnazyme chemiluminescence biosensing platform for on-site detection of nucleic acid and non-nucleic acid targets","volume":"247","author":"Chen","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.bsheal.2024.09.005_b0420","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342514","article-title":"A rapid and sensitive fluorescent chromatography with cloud system for MPXV point-of-care diagnosis","volume":"1302","author":"Yan","year":"2024","journal-title":"Anal. Chim. Acta"},{"issue":"2","key":"10.1016\/j.bsheal.2024.09.005_b0425","doi-asserted-by":"crossref","first-page":"405","DOI":"10.4269\/ajtmh.16-0567","article-title":"Evaluation of the genexpert for human monkeypox diagnosis","volume":"96","author":"Li","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"22","key":"10.1016\/j.bsheal.2024.09.005_b0430","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.clinmicnews.2022.11.003","article-title":"Monkeypox: clinical considerations, epidemiology, and laboratory diagnostics","volume":"44","author":"Osborn","year":"2022","journal-title":"Clin. Microbiol. Newsl."},{"issue":"12","key":"10.1016\/j.bsheal.2024.09.005_b0435","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.29261","article-title":"Serological methods for the detection of antibodies against monkeypox virus applicable for laboratories with different biosafety levels","volume":"95","author":"Grossegesse","year":"2023","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.bsheal.2024.09.005_b0440","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2023.114836","article-title":"Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus","volume":"322","author":"Therrien","year":"2023","journal-title":"J. Virol. Methods"},{"issue":"10","key":"10.1016\/j.bsheal.2024.09.005_b0445","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.29134","article-title":"Development of a novel serological assay for the detection of mpox infection in vaccinated populations","volume":"95","author":"Yates","year":"2023","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.bsheal.2024.09.005_b0450","doi-asserted-by":"crossref","first-page":"1243523","DOI":"10.3389\/fmicb.2023.1243523","article-title":"Development of a specific MPXV antigen detection immunodiagnostic assay","volume":"14","author":"Davis","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.bsheal.2024.09.005_b0455","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2023.132136","article-title":"Molybdenum disulfide-loaded multilayer aunps with colorimetric-sers dual-signal enhancement activities for flexible immunochromatographic diagnosis of monkeypox virus","volume":"459","author":"Yu","year":"2023","journal-title":"J. Hazard. Mater."},{"issue":"1","key":"10.1016\/j.bsheal.2024.09.005_b0460","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/s12951-023-02215-4","article-title":"Fluorescence-enhanced dual signal lateral flow immunoassay for flexible and ultrasensitive detection of monkeypox virus","volume":"21","author":"Yang","year":"2023","journal-title":"J. Nanobiotechnology"},{"issue":"2","key":"10.1016\/j.bsheal.2024.09.005_b0465","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1006\/viro.2002.1446","article-title":"Analysis of the monkeypox virus genome","volume":"297","author":"Shchelkunov","year":"2002","journal-title":"Virology"},{"key":"10.1016\/j.bsheal.2024.09.005_b0470","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2024.1414224","article-title":"Exploring monkeypox virus proteins and rapid detection techniques","volume":"14","author":"Sagdat","year":"2024","journal-title":"Front. Cell Infect. Microbiol."},{"issue":"6","key":"10.1016\/j.bsheal.2024.09.005_b0475","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.3390\/v14061224","article-title":"New p35 (h3l) epitope involved in vaccinia virus neutralization and its deimmunization","volume":"14","author":"Khlusevich","year":"2022","journal-title":"Viruses"},{"issue":"2","key":"10.1016\/j.bsheal.2024.09.005_b0480","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.105957","article-title":"Monkeypox infection elicits strong antibody and B cell response against a35r and h3l antigens","volume":"26","author":"Yefet","year":"2023","journal-title":"Iscience"},{"issue":"1","key":"10.1016\/j.bsheal.2024.09.005_b0485","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.virs.2022.10.004","article-title":"Immunization of mice with vaccinia virus tiantan strain yields antibodies cross-reactive with protective antigens of monkeypox virus","volume":"38","author":"Yang","year":"2023","journal-title":"Virol. Sin."},{"issue":"8472","key":"10.1016\/j.bsheal.2024.09.005_b0490","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/S0140-6736(86)90748-8","article-title":"Isolation of monkeypox virus from wild squirrel infected in nature","volume":"1","author":"Khodakevich","year":"1986","journal-title":"Lancet"},{"key":"10.1016\/j.bsheal.2024.09.005_b0495","article-title":"Cases of monkeypox show highly-overlapping co-infection with HIV and syphilis","volume":"11","author":"Jia","year":"2023","journal-title":"Front. 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