{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:23:14Z","timestamp":1785338594737,"version":"3.55.0"},"reference-count":62,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,3,21]],"date-time":"2018-03-21T00:00:00Z","timestamp":1521590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research (NHMRC)","doi-asserted-by":"publisher","award":["APP1046711"],"award-info":[{"award-number":["APP1046711"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Western Australia Cancer Council"},{"name":"Western Australia Cancer Council Suzanne Cavanagh Early Career Investigator","award":["2016"],"award-info":[{"award-number":["2016"]}]},{"DOI":"10.13039\/501100001798","name":"Edith Cowan University Early Career Research","doi-asserted-by":"publisher","award":["2016"],"award-info":[{"award-number":["2016"]}],"id":[{"id":"10.13039\/501100001798","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cancer Research Trust"},{"name":"Western Australian Department of Health Research Translation"},{"name":"Spinnaker"},{"DOI":"10.13039\/501100001798","name":"ECU","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001798","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cancer Letters"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.canlet.2018.03.013","type":"journal-article","created":{"date-parts":[[2018,3,18]],"date-time":"2018-03-18T15:41:27Z","timestamp":1521387687000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"special_numbering":"C","title":["Melanoma circulating tumor cells: Benefits and challenges required for clinical application"],"prefix":"10.1016","volume":"424","author":[{"given":"G.","family":"Marsavela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.A.","family":"Aya-Bonilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.E.","family":"Warkiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8613-3570","authenticated-orcid":false,"given":"E.S.","family":"Gray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ziman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.canlet.2018.03.013_bib1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1158\/2159-8290.CD-15-1483","article-title":"Clinical applications of circulating tumor cells and circulating tumor DNA as liquid biopsy","volume":"6","author":"Alix-Panabieres","year":"2016","journal-title":"Canc. Discov."},{"key":"10.1016\/j.canlet.2018.03.013_bib2","doi-asserted-by":"crossref","first-page":"6897","DOI":"10.1158\/1078-0432.CCR-04-0378","article-title":"Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases","volume":"10","author":"Allard","year":"2004","journal-title":"Clin. Canc. Res.: Off. J. Am. Ass. Canc. Res."},{"issue":"2","key":"10.1016\/j.canlet.2018.03.013_bib3","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S1470-2045(18)30015-9","article-title":"Neoadjuvant plus adjuvant dabrafenib and trametinib versus standard of care in patients with high-risk, surgically resectable melanoma: a single-centre, open-label, randomised, phase 2 trial","volume":"19","author":"Amaria","year":"2018","journal-title":"Lancet Oncol."},{"issue":"40","key":"10.1016\/j.canlet.2018.03.013_bib4","doi-asserted-by":"crossref","first-page":"67355","DOI":"10.18632\/oncotarget.18641","article-title":"Isolation and detection of circulating tumour cells from metastatic melanoma patients using a slanted spiral microfluidic device","volume":"8","author":"Aya-Bonilla","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.canlet.2018.03.013_bib5","series-title":"Cutaneous Melanoma","author":"Balch","year":"2009"},{"key":"10.1016\/j.canlet.2018.03.013_bib6","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1615\/CritRevImmunol.v24.i4.40","article-title":"Human high molecular weight-melanoma-associated antigen (HMW-MAA): a melanoma cell surface chondroitin sulfate proteoglycan (MSCP) with biological and clinical significance","volume":"24","author":"Campoli","year":"2004","journal-title":"Crit. Rev. Immunol."},{"key":"10.1016\/j.canlet.2018.03.013_bib7","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/j.cell.2015.05.044","article-title":"Genomic classification of cutaneous melanoma","volume":"161","author":"Cancer Genome Atlas Network","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2018.03.013_bib8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1097\/CMR.0b013e3283252feb","article-title":"Cytokeratin expression in malignant melanoma: potential application of in-situ hybridization analysis of mRNA","volume":"19","author":"Chen","year":"2009","journal-title":"Melanoma Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib9","doi-asserted-by":"crossref","first-page":"e41052","DOI":"10.1371\/journal.pone.0041052","article-title":"Circulating tumor cells in melanoma patients","volume":"7","author":"Clawson","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.canlet.2018.03.013_bib10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3816\/CCC.2006.n.029","article-title":"Isolation and characterization of circulating tumor cells in patients with metastatic colorectal cancer","volume":"6","author":"Cohen","year":"2006","journal-title":"Clin. Colorectal Canc."},{"key":"10.1016\/j.canlet.2018.03.013_bib11","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1053\/j.seminoncol.2006.03.016","article-title":"Circulating tumor cells, disease progression, and survival in metastatic breast cancer","volume":"33","author":"Cristofanilli","year":"2006","journal-title":"Semin. Oncol."},{"key":"10.1016\/j.canlet.2018.03.013_bib12","doi-asserted-by":"crossref","first-page":"7053","DOI":"10.1158\/1078-0432.CCR-07-1506","article-title":"Circulating tumor cell number and prognosis in progressive castration-resistant prostate cancer","volume":"13","author":"Danila","year":"2007","journal-title":"Clin. Canc. Res.: Off. J. Am. Ass. Canc. Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib13","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1186\/1476-4598-9-301","article-title":"Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP)-targeted delivery of soluble TRAIL potently inhibits melanoma outgrowth in\u00a0vitro and in\u00a0vivo","volume":"9","author":"de Bruyn","year":"2010","journal-title":"Mol. Canc."},{"key":"10.1016\/j.canlet.2018.03.013_bib14","article-title":"Application of a filtration and isolation by size technique for the detection of circulating tumor cells in cutaneous melanoma","volume":"64","author":"De Giorgi","year":"2011","journal-title":"J.\u00a0Am. Acad. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib15","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1001\/archdermatol.2010.264","article-title":"Circulating benign nevus cells detected by ISET technique: warning for melanoma molecular diagnosis","volume":"146","author":"De Giorgi","year":"2010","journal-title":"Arch. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib16","doi-asserted-by":"crossref","first-page":"2440","DOI":"10.1038\/jid.2010.141","article-title":"Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma","volume":"130","author":"De Giorgi","year":"2010","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib17","first-page":"3223","article-title":"Heterogeneous expression of immunotherapy candidate proteins gp100, MART-1, and tyrosinase in human melanoma cell lines and in human melanocytic lesions","volume":"57","author":"de Vries","year":"1997","journal-title":"Canc. Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2017.01.026","article-title":"SnapShot: circulating tumor cells","volume":"168","author":"Dive","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2018.03.013_bib19","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1038\/sj.onc.1206460","article-title":"Development of melanocyte precursors from the vertebrate neural crest","volume":"22","author":"Dupin","year":"2003","journal-title":"Oncogene"},{"key":"10.1016\/j.canlet.2018.03.013_bib20","doi-asserted-by":"crossref","first-page":"4320","DOI":"10.1158\/0008-5472.CAN-04-3327","article-title":"ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma","volume":"65","author":"Frank","year":"2005","journal-title":"Canc. Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib21","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/1479-5876-10-192","article-title":"Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells","volume":"10","author":"Freeman","year":"2012","journal-title":"J.\u00a0Transl. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib22","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/jid.2010.285","article-title":"Expression of the stem cell markers nestin and CD133 on circulating melanoma cells","volume":"131","author":"Fusi","year":"2011","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib23","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1158\/2159-8290.CD-15-1336","article-title":"Application of sequencing, liquid biopsies, and patient-derived xenografts for personalized medicine in melanoma","volume":"6","author":"Girotti","year":"2016","journal-title":"Canc. Discov."},{"key":"10.1016\/j.canlet.2018.03.013_bib24","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/j.ebiom.2015.09.029","article-title":"Arterial or venous: where are the circulating tumor cells?","volume":"2","author":"Gray","year":"2015","journal-title":"EBioMedicine"},{"key":"10.1016\/j.canlet.2018.03.013_bib25","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1038\/jid.2015.127","article-title":"Circulating melanoma cell subpopulations: their heterogeneity and differential responses to treatment","volume":"135","author":"Gray","year":"2015","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib26","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1097\/SLA.0b013e3182380f56","article-title":"Assessment of prognostic circulating tumor cells in a phase III trial of adjuvant immunotherapy after complete resection of stage IV melanoma","volume":"255","author":"Hoshimoto","year":"2012","journal-title":"Ann. Surg."},{"key":"10.1016\/j.canlet.2018.03.013_bib27","first-page":"101","article-title":"PD-L1 expression on Circulating Melanoma Cells is predictive of response to Pembrolizumab","volume":"30","author":"Khattak","year":"2017","journal-title":"Pigment Cell Melanoma Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib28","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1038\/jid.2012.468","article-title":"Biomarker utility of circulating tumor cells in metastatic cutaneous melanoma","volume":"133","author":"Khoja","year":"2013","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib29","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1097\/CMR.0000000000000025","article-title":"Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma","volume":"24","author":"Khoja","year":"2014","journal-title":"Melanoma Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib30","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1373\/clinchem.2008.116467","article-title":"mRNA expression and BRAF mutation in circulating melanoma cells isolated from peripheral blood with high molecular weight melanoma-associated antigen-specific monoclonal antibody beads","volume":"55","author":"Kitago","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.canlet.2018.03.013_bib31","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1186\/1471-2407-14-423","article-title":"Monitoring changes in circulating tumour cells as a prognostic indicator of overall survival and treatment response in patients with metastatic melanoma","volume":"14","author":"Klinac","year":"2014","journal-title":"BMC Canc."},{"key":"10.1016\/j.canlet.2018.03.013_bib32","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1373\/clinchem.2004.045096","article-title":"Multimarker quantitative real-time PCR detection of circulating melanoma cells in peripheral blood: relation to disease stage in melanoma patients","volume":"51","author":"Koyanagi","year":"2005","journal-title":"Clin. Chem."},{"key":"10.1016\/j.canlet.2018.03.013_bib33","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1038\/jid.2010.377","article-title":"RANK is expressed in metastatic melanoma and highly upregulated on melanoma-initiating cells","volume":"131","author":"Kupas","year":"2011","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib34","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/1479-5876-9-70","article-title":"Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients","volume":"9","author":"Liu","year":"2011","journal-title":"J.\u00a0Transl. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib35","first-page":"463","article-title":"Targeted agents and immunotherapies: optimizing outcomes in melanoma, Nature reviews","volume":"14","author":"Luke","year":"2017","journal-title":"Clin. Oncol."},{"key":"10.1016\/j.canlet.2018.03.013_bib36","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.celrep.2014.03.039","article-title":"Isolation and molecular characterization of circulating melanoma cells","volume":"7","author":"Luo","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.canlet.2018.03.013_bib37","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1038\/ncb1824","article-title":"Nuclear signalling by tumour-associated antigen EpCAM","volume":"11","author":"Maetzel","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.canlet.2018.03.013_bib38","doi-asserted-by":"crossref","first-page":"5627","DOI":"10.1158\/0008-5472.CAN-09-0654","article-title":"The emerging role of EpCAM in cancer and stem cell signaling","volume":"69","author":"Munz","year":"2009","journal-title":"Canc. Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1515\/CCLM.2009.009","article-title":"Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance","volume":"47","author":"Nezos","year":"2009","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib40","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1093\/annonc\/mdq013","article-title":"Efficacy and safety of ipilimumab monotherapy in patients with pretreated advanced melanoma: a multicenter single-arm phase II study","volume":"21","author":"O'Day","year":"2010","journal-title":"Ann. Oncol."},{"key":"10.1016\/j.canlet.2018.03.013_bib41","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3005616","article-title":"Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells","volume":"5","author":"Ozkumur","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib42","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.canlet.2006.12.014","article-title":"Circulating tumor cells (CTC) detection: clinical impact and future directions","volume":"253","author":"Paterlini-Brechot","year":"2007","journal-title":"Canc. Lett."},{"key":"10.1016\/j.canlet.2018.03.013_bib43","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/modpathol.3801019","article-title":"PAX3 expression in primary melanomas and nevi","volume":"21","author":"Plummer","year":"2008","journal-title":"Mod. Pathol.: Off. J. United States Can. Acad. Pathol., Inc"},{"key":"10.1016\/j.canlet.2018.03.013_bib44","first-page":"755","article-title":"Circulating melanoma cells and survival in metastatic melanoma","volume":"38","author":"Rao","year":"2011","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.canlet.2018.03.013_bib45","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.clinbiochem.2014.12.007","article-title":"Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR","volume":"48","author":"Reid","year":"2015","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.canlet.2018.03.013_bib46","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/bjd.12057","article-title":"Markers of circulating tumour cells in the peripheral blood of patients with melanoma correlate with disease recurrence and progression","volume":"168","author":"Reid","year":"2013","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.canlet.2018.03.013_bib47","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1158\/1078-0432.CCR-13-3122","article-title":"BRAF inhibitor resistance mechanisms in metastatic melanoma: spectrum and clinical impact","volume":"20","author":"Rizos","year":"2014","journal-title":"Clin. Canc. Res."},{"key":"10.1016\/j.canlet.2018.03.013_bib48","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1056\/NEJMoa1412082","article-title":"nivolumab in previously untreated melanoma without BRAF mutation","volume":"372","author":"Robert","year":"2015","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib49","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1038\/onc.2014.249","article-title":"Tumor heterogeneity and plasticity as elusive drivers for resistance to MAPK pathway inhibition in melanoma","volume":"34","author":"Roesch","year":"2015","journal-title":"Oncogene"},{"key":"10.1016\/j.canlet.2018.03.013_bib50","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1002\/cyto.10161","article-title":"Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood","volume":"49","author":"Rosenberg","year":"2002","journal-title":"Cytometry"},{"key":"10.1016\/j.canlet.2018.03.013_bib51","doi-asserted-by":"crossref","first-page":"016008","DOI":"10.1088\/1478-3975\/12\/1\/016008","article-title":"Limited genomic heterogeneity of circulating melanoma cells in advanced stage patients","volume":"12","author":"Ruiz","year":"2015","journal-title":"Phys. Biol."},{"key":"10.1016\/j.canlet.2018.03.013_bib52","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1038\/bjc.2012.12","article-title":"Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level","volume":"106","author":"Sakaizawa","year":"2012","journal-title":"Br. J. Canc."},{"key":"10.1016\/j.canlet.2018.03.013_bib53","first-page":"685","article-title":"A\u00a0microfluidic device for label-free, physical capture of circulating tumor cell clusters","volume":"12","author":"Sarioglu","year":"2015","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.canlet.2018.03.013_bib54","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1158\/2159-8290.CD-13-0642","article-title":"Acquired resistance and clonal evolution in melanoma during BRAF inhibitor therapy","volume":"4","author":"Shi","year":"2014","journal-title":"Canc. Discov."},{"key":"10.1016\/j.canlet.2018.03.013_bib55","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/08998280.2008.11928377","article-title":"Circulating tumor cells in melanoma: a review of the literature and description of a novel technique","volume":"21","author":"Steen","year":"2008","journal-title":"Proc. (Baylor Univ. Med. Center)"},{"key":"10.1016\/j.canlet.2018.03.013_bib56","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1126\/science.aad0501","article-title":"Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq","volume":"352","author":"Tirosh","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.canlet.2018.03.013_bib57","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1056\/NEJMoa1200690","article-title":"Safety, activity, and immune correlates of anti\u2013pd-1 antibody in cancer","volume":"366","author":"Topalian","year":"2012","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.canlet.2018.03.013_bib58","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0002-9440(10)64706-2","article-title":"Isolation by size of epithelial tumor cells: a new method for the immunomorphological and molecular characterization of circulating tumor cells","volume":"156","author":"Vona","year":"2000","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.canlet.2018.03.013_bib59","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1039\/C3LC50617G","article-title":"Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells","volume":"14","author":"Warkiani","year":"2014","journal-title":"Lab Chip"},{"key":"10.1016\/j.canlet.2018.03.013_bib60","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1038\/nprot.2016.003","article-title":"Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics","volume":"11","author":"Warkiani","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2018.03.013_bib61","doi-asserted-by":"crossref","first-page":"e0123376","DOI":"10.1371\/journal.pone.0123376","article-title":"A\u00a0novel approach for the detection and genetic analysis of live melanoma circulating tumor cells","volume":"10","author":"Xu","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.canlet.2018.03.013_bib62","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1038\/jid.2010.215","article-title":"Circulating tumor cells and melanoma progression","volume":"130","author":"Xu","year":"2010","journal-title":"J.\u00a0Invest. Dermatol."}],"container-title":["Cancer Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383518302040?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383518302040?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T23:44:54Z","timestamp":1565307894000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304383518302040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":62,"alternative-id":["S0304383518302040"],"URL":"https:\/\/doi.org\/10.1016\/j.canlet.2018.03.013","relation":{},"ISSN":["0304-3835"],"issn-type":[{"value":"0304-3835","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Melanoma circulating tumor cells: Benefits and challenges required for clinical application","name":"articletitle","label":"Article Title"},{"value":"Cancer Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.canlet.2018.03.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}