{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:35:08Z","timestamp":1790782508783,"version":"4.1.0"},"reference-count":164,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009330","name":"Medical Science and Technology Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2020B1111160001"],"award-info":[{"award-number":["2020B1111160001"]}],"id":[{"id":"10.13039\/501100009330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009330","name":"Medical Science and Technology Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515220084"],"award-info":[{"award-number":["2021A1515220084"]}],"id":[{"id":"10.13039\/501100009330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515110931"],"award-info":[{"award-number":["2021A1515110931"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["201906133000069"],"award-info":[{"award-number":["201906133000069"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["GJHZ20200731095604013"],"award-info":[{"award-number":["GJHZ20200731095604013"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["JCYJ20210324103407018"],"award-info":[{"award-number":["JCYJ20210324103407018"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["JCYJ20220530163206015"],"award-info":[{"award-number":["JCYJ20220530163206015"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["JSGG20200807171602031"],"award-info":[{"award-number":["JSGG20200807171602031"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["SGLH20180625171602058"],"award-info":[{"award-number":["SGLH20180625171602058"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017380","name":"Science and Technology Foundation of Shenzhen City","doi-asserted-by":"publisher","award":["ZDSYS20210623092001003"],"award-info":[{"award-number":["ZDSYS20210623092001003"]}],"id":[{"id":"10.13039\/100017380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203870"],"award-info":[{"award-number":["82203870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002402","name":"Sun Yat-sen University","doi-asserted-by":"publisher","award":["2019kfkt06"],"award-info":[{"award-number":["2019kfkt06"]}],"id":[{"id":"10.13039\/501100002402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cancer Letters"],"published-print":{"date-parts":[[2023,2]]},"DOI":"10.1016\/j.canlet.2022.216048","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T11:16:22Z","timestamp":1672658182000},"page":"216048","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":62,"special_numbering":"C","title":["Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy"],"prefix":"10.1016","volume":"555","author":[{"given":"Lichan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiafei","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayong","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6755-6482","authenticated-orcid":false,"given":"Yong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.canlet.2022.216048_bib1","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1053\/j.gastro.2009.05.044","article-title":"Stem cells and liver regeneration","volume":"137","author":"Duncan","year":"2009","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2022.216048_bib2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jhep.2018.09.014","article-title":"Burden of liver diseases in the world","volume":"70","author":"Asrani","year":"2019","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, CA","volume":"68","author":"Bray","year":"2018","journal-title":"Cancer J. Clin."},{"key":"10.1016\/j.canlet.2022.216048_bib4","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1016\/j.celrep.2018.07.001","article-title":"Organoid models of human liver cancers derived from tumor needle biopsies","volume":"24","author":"Nuciforo","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/s12916-018-1110-y","article-title":"Management of patients with liver diseases on the waiting list for transplantation: a major impact to the success of liver transplantation","volume":"16","author":"Samuel","year":"2018","journal-title":"BMC Med."},{"key":"10.1016\/j.canlet.2022.216048_bib6","first-page":"266","article-title":"Modeling physiological events in 2D vs","volume":"32","author":"Pegoraro","year":"2017","journal-title":"3D Cell Cult. Physiol. Bethesda Md"},{"key":"10.1016\/j.canlet.2022.216048_bib7","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3389\/fcell.2019.00345","article-title":"Next-generation liver medicine using organoid models","volume":"7","author":"Akbari","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.canlet.2022.216048_bib8","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s13770-021-00357-w","article-title":"Liver organoids: formation strategies and biomedical applications","volume":"18","author":"Zhang","year":"2021","journal-title":"Tissue Eng. Regen. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib9","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.eng.2018.07.021","article-title":"A review of 3D printing technology for medical applications","volume":"4","author":"Yan","year":"2018","journal-title":"Engineering"},{"key":"10.1016\/j.canlet.2022.216048_bib10","unstructured":"Organogenesis in a dish: modeling development and disease using organoid technologies, (n.d.). https:\/\/www.science.org\/doi\/10.1126\/science.1247125?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed (accessed March 8, 2022)."},{"key":"10.1016\/j.canlet.2022.216048_bib11","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/j.cell.2016.05.082","article-title":"Modeling development and disease with organoids","volume":"165","author":"Clevers","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib12","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.7150\/thno.66670","article-title":"Making human pancreatic islet organoids: progresses on the cell origins, biomaterials and three-dimensional technologies","volume":"12","author":"Jiang","year":"2022","journal-title":"Theranostics"},{"key":"10.1016\/j.canlet.2022.216048_bib13","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1146\/annurev-pathmechdis-012419-032611","article-title":"Human organoids: tools for understanding biology and treating diseases","volume":"15","author":"Schutgens","year":"2020","journal-title":"Annu. Rev. Pathol."},{"key":"10.1016\/j.canlet.2022.216048_bib14","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1016\/j.transproceed.2012.02.007","article-title":"Self-organization of human hepatic organoid by recapitulating organogenesis in vitro","volume":"44","author":"Takebe","year":"2012","journal-title":"Transplant. Proc."},{"key":"10.1016\/j.canlet.2022.216048_bib15","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nature12271","article-title":"Vascularized and functional human liver from an iPSC-derived organ bud transplant","volume":"499","author":"Takebe","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib16","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nature11826","article-title":"In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration","volume":"494","author":"Huch","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib17","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nbt.3275","article-title":"Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation","volume":"33","author":"Sampaziotis","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.canlet.2022.216048_bib18","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1038\/nprot.2016.097","article-title":"Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation","volume":"11","author":"Broutier","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2022.216048_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/srep21174","article-title":"Three-dimensional organotypic culture models of human hepatocellular carcinoma","volume":"6","author":"Takai","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.canlet.2021.02.024","article-title":"3D human liver organoids: an in vitro platform to investigate HBV infection, replication and liver tumorigenesis","volume":"506","author":"Rao","year":"2021","journal-title":"Cancer Lett."},{"key":"10.1016\/j.canlet.2022.216048_bib21","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1038\/nm.4438","article-title":"Human primary liver cancer\u2013derived organoid cultures for disease modeling and drug screening","volume":"23","author":"Broutier","year":"2017","journal-title":"Nat. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib22","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/j.cell.2018.11.013","article-title":"Long-term expansion of functional mouse and human hepatocytes as 3D organoids","volume":"175","author":"Hu","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib23","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.jhep.2019.06.030","article-title":"Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids","volume":"71","author":"Mun","year":"2019","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib24","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.stemcr.2019.08.007","article-title":"Robust, long-term culture of endoderm-derived hepatic organoids for disease modeling","volume":"13","author":"Akbari","year":"2019","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib25","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.celrep.2019.03.088","article-title":"Establishment of patient-derived organoids and drug screening for biliary tract carcinoma","volume":"27","author":"Saito","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib26","doi-asserted-by":"crossref","unstructured":"L. Li, H. Knutsdottir, K. Hui, M.J. Weiss, J. He, B. Philosophe, A.M. Cameron, C.L. Wolfgang, T.M. Pawlik, G. Ghiaur, A.J. Ewald, E. Mezey, J.S. Bader, F.M. Selaru, Human primary liver cancer organoids reveal intratumor and interpatient drug response heterogeneity, JCI Insight. 4 (n.d.) e121490. https:\/\/doi.org\/10.1172\/jci.insight.121490.","DOI":"10.1172\/jci.insight.121490"},{"key":"10.1016\/j.canlet.2022.216048_bib27","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.cmet.2019.05.007","article-title":"Modeling steatohepatitis in humans with pluripotent stem cell-derived organoids","volume":"30","author":"Ouchi","year":"2019","journal-title":"Cell Metabol."},{"key":"10.1016\/j.canlet.2022.216048_bib28","doi-asserted-by":"crossref","first-page":"e9968","DOI":"10.7717\/peerj.9968","article-title":"Generation of human liver organoids from pluripotent stem cell-derived hepatic endoderms","volume":"8","author":"Kulkeaw","year":"2020","journal-title":"PeerJ"},{"key":"10.1016\/j.canlet.2022.216048_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2020.119823","article-title":"Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model","volume":"237","author":"Elbadawy","year":"2020","journal-title":"Biomaterials"},{"key":"10.1016\/j.canlet.2022.216048_bib30","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1053\/j.gastro.2020.06.010","article-title":"Human pluripotent stem cell-derived organoids as models of liver disease","volume":"159","author":"Ramli","year":"2020","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2022.216048_bib31","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.3390\/cancers12092668","article-title":"A human organoid model of aggressive hepatoblastoma for disease modeling and drug testing","volume":"12","author":"Saltsman","year":"2020","journal-title":"Cancers"},{"key":"10.1016\/j.canlet.2022.216048_bib32","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.jcmgh.2020.09.003","article-title":"Cancer-associated fibroblasts provide a stromal niche for liver cancer organoids that confers trophic effects and therapy resistance","volume":"11","author":"Liu","year":"2020","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib33","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-52204-7","article-title":"Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells","volume":"9","author":"W\u00f6rsd\u00f6rfer","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib34","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.canlet.2021.03.017","article-title":"Developing liver organoids from induced pluripotent stem cells (iPSCs): an alternative source of organoid generation for liver cancer research","volume":"508","author":"Nguyen","year":"2021","journal-title":"Cancer Lett."},{"key":"10.1016\/j.canlet.2022.216048_bib35","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/s41596-020-00411-2","article-title":"Establishment of human fetal hepatocyte organoids and CRISPR\u2013Cas9-based gene knockin and knockout in organoid cultures from human liver","volume":"16","author":"Hendriks","year":"2021","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2022.216048_bib36","doi-asserted-by":"crossref","unstructured":"Y. Guan, D. Xu, P.M. Garfin, U. Ehmer, M. Hurwitz, G. Enns, S. Michie, M. Wu, M. Zheng, T. Nishimura, J. Sage, G. Peltz, Human hepatic organoids for the analysis of human genetic diseases, JCI Insight. 2 (n.d.) e94954. https:\/\/doi.org\/10.1172\/jci.insight.94954.","DOI":"10.1172\/jci.insight.94954"},{"key":"10.1016\/j.canlet.2022.216048_bib37","doi-asserted-by":"crossref","first-page":"6138","DOI":"10.1038\/s41467-021-26410-9","article-title":"A human multi-lineage hepatic organoid model for liver fibrosis","volume":"12","author":"Guan","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.canlet.2022.216048_bib38","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.3390\/cancers13163997","article-title":"A novel orthotopic liver cancer model for creating a human-like tumor microenvironment","volume":"13","author":"Qiu","year":"2021","journal-title":"Cancers"},{"key":"10.1016\/j.canlet.2022.216048_bib39","doi-asserted-by":"crossref","DOI":"10.1002\/btm2.10207","article-title":"Biofabricated 3D in vitro model of fibrosis\u2010induced abnormal hepatoblast\/biliary progenitors' expansion of the developing liver","volume":"6","author":"Brovold","year":"2021","journal-title":"Bioeng. Transl. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib40","article-title":"Recapitulating hepatitis E virus\u2013host interactions and facilitating antiviral drug discovery in human liver\u2013derived organoids","volume":"8","author":"Li","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.canlet.2022.216048_bib41","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/hep.32107","article-title":"Biliary organoids uncover delayed epithelial development and barrier function in biliary atresia","volume":"75","author":"Amarachintha","year":"2022","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2022.216048_bib42","doi-asserted-by":"crossref","first-page":"644","DOI":"10.3390\/cimb44020045","article-title":"Developing biliary atresia-like model by treating human liver organoids with polyinosinic:polycytidylic acid (poly (I:C))","volume":"44","author":"Chung","year":"2022","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.canlet.2022.216048_bib43","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s00109-021-02176-x","article-title":"Recapitulating lipid accumulation and related metabolic dysregulation in human liver-derived organoids","volume":"100","author":"Wang","year":"2022","journal-title":"J. Mol. Med. (Limerick)"},{"key":"10.1016\/j.canlet.2022.216048_bib44","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s10020-022-00498-1","article-title":"Kinome profiling of cholangiocarcinoma organoids reveals potential druggable targets that hold promise for treatment stratification","volume":"28","author":"Lieshout","year":"2022","journal-title":"Mol. Med. Camb. Mass."},{"key":"10.1016\/j.canlet.2022.216048_bib45","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1126\/science.aaw6985","article-title":"Cancer modeling meets human organoid technology","volume":"364","author":"Tuveson","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.canlet.2022.216048_bib46","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1093\/carcin\/bgy129","article-title":"Modeling liver cancer and therapy responsiveness using organoids derived from primary mouse liver tumors","volume":"40","author":"Cao","year":"2019","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.canlet.2022.216048_bib47","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1038\/s41467-020-15846-0","article-title":"LGR5 marks targetable tumor-initiating cells in mouse liver cancer","volume":"11","author":"Cao","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.canlet.2022.216048_bib48","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232012327","article-title":"Cancer stem cells in hepatocellular carcinoma: intrinsic and extrinsic molecular mechanisms in stemness regulation","volume":"23","author":"Fang","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib49","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.canlet.2020.10.012","article-title":"Cancer-initiating cells in human pancreatic cancer organoids are maintained by interactions with endothelial cells","volume":"498","author":"Choi","year":"2021","journal-title":"Cancer Lett."},{"key":"10.1016\/j.canlet.2022.216048_bib50","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.stem.2020.09.014","article-title":"Pluripotent stem cell-based cell therapy-promise and challenges","volume":"27","author":"Yamanaka","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib51","doi-asserted-by":"crossref","unstructured":"E. De Crignis, T. Hossain, S. Romal, F. Carofiglio, P. Moulos, M.M. Khalid, S. Rao, A. Bazrafshan, M.M. Verstegen, F. Pourfarzad, C. Koutsothanassis, H. Gehart, T.W. Kan, R.-J. Palstra, C. Boucher, J.N. Ijzermans, M. Huch, S.F. Boj, R. Vries, H. Clevers, L.J. van der Laan, P. Hatzis, T. Mahmoudi, Application of human liver organoids as a patient-derived primary model for HBV infection and related hepatocellular carcinoma, Elife. 10 (n.d.) e60747. https:\/\/doi.org\/10.7554\/eLife.60747.","DOI":"10.7554\/eLife.60747"},{"key":"10.1016\/j.canlet.2022.216048_bib52","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.748576","article-title":"Challenges for the applications of human pluripotent stem cell-derived liver organoids","volume":"9","author":"Chang","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.canlet.2022.216048_bib53","article-title":"A combined model of human iPSC\u2010derived liver organoids and hepatocytes reveals ferroptosis in DGUOK mutant mtDNA depletion syndrome","volume":"8","author":"Guo","year":"2021","journal-title":"Adv. Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib54","doi-asserted-by":"crossref","first-page":"6215","DOI":"10.3390\/ijms21176215","article-title":"iPSC-derived liver organoids: a journey from drug screening, to disease modeling, arriving to regenerative medicine","volume":"21","author":"Olgasi","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib55","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/j.bbadis.2018.08.038","article-title":"Liver diseases in the dish: iPSC and organoids as a new approach to modeling liver diseases","volume":"1865","author":"Fiorotto","year":"2019","journal-title":"Biochim. Biophys. Acta, Mol. Basis Dis."},{"key":"10.1016\/j.canlet.2022.216048_bib56","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3390\/jcm10010036","article-title":"In Vitro and in vivo models of non-alcoholic fatty liver disease: a critical appraisal","volume":"10","author":"Soret","year":"2020","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib58","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.jhep.2014.01.021","article-title":"Guidelines for the diagnosis and management of intrahepatic cholangiocarcinoma","volume":"60","author":"Bridgewater","year":"2014","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib59","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/s41572-020-00240-3","article-title":"Hepatocellular carcinoma","volume":"7","author":"Llovet","year":"2021","journal-title":"Nat. Rev. Dis. Prim."},{"key":"10.1016\/j.canlet.2022.216048_bib60","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial\u2013mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.canlet.2022.216048_bib61","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1038\/nprot.2016.097","article-title":"Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation","volume":"11","author":"Broutier","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2022.216048_bib62","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.cell.2014.12.021","article-title":"Organoid models of human and mouse ductal pancreatic cancer","volume":"160","author":"Boj","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib63","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.cell.2015.03.053","article-title":"Prospective derivation of a Living Organoid Biobank of colorectal cancer patients","volume":"161","author":"van de Wetering","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib64","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1038\/s41556-019-0359-5","article-title":"Modelling liver cancer initiation with organoids derived from directly reprogrammed human hepatocytes","volume":"21","author":"Sun","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.canlet.2022.216048_bib65","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/s41576-018-0051-9","article-title":"Progress and potential in organoid research","volume":"19","author":"Rossi","year":"2018","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.canlet.2022.216048_bib66","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.201901658","article-title":"Cellulose nanofibril hydrogel promotes hepatic differentiation of human liver organoids","volume":"9","author":"Kr\u00fcger","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.canlet.2022.216048_bib67","doi-asserted-by":"crossref","first-page":"6235","DOI":"10.1073\/pnas.1015938108","article-title":"Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer","volume":"108","author":"Kondo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.canlet.2022.216048_bib68","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.stem.2016.04.003","article-title":"A colorectal tumor organoid library demonstrates progressive loss of niche factor requirements during tumorigenesis","volume":"18","author":"Fujii","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib69","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.cell.2014.08.016","article-title":"Organoid cultures derived from patients with advanced prostate cancer","volume":"159","author":"Gao","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib70","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1038\/nm.3973","article-title":"Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids","volume":"21","author":"Huang","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib71","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.cell.2017.11.010","article-title":"A living biobank of breast cancer organoids captures disease heterogeneity","volume":"172","author":"Sachs","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib72","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/s41568-018-0007-6","article-title":"Organoids in cancer research","volume":"18","author":"Drost","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.canlet.2022.216048_bib73","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.stem.2020.06.015","article-title":"A human pluripotent stem cell-based platform to study SARS-CoV-2 tropism and model virus infection in human cells and organoids","volume":"27","author":"Yang","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib74","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.cell.2014.11.050","article-title":"Long-term culture of genome-stable bipotent stem cells from adult human liver","volume":"160","author":"Huch","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib75","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1053\/j.gastro.2017.11.002","article-title":"Mouse model of Alagille syndrome and mechanisms of Jagged1 missense mutations","volume":"154","author":"Andersson","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2022.216048_bib76","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/nbt.3294","article-title":"Directed differentiation of cholangiocytes from human pluripotent stem cells","volume":"33","author":"Ogawa","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.canlet.2022.216048_bib77","doi-asserted-by":"crossref","first-page":"410","DOI":"10.3390\/cells9020410","article-title":"Long-term survival of transplanted autologous canine liver organoids in a COMMD1-deficient dog model of metabolic liver disease","volume":"9","author":"Kruitwagen","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.canlet.2022.216048_bib78","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.stemcr.2015.09.002","article-title":"Disease modeling and gene therapy of copper storage disease in canine hepatic organoids","volume":"5","author":"Nantasanti","year":"2015","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib79","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/j.stemcr.2017.02.015","article-title":"Long-term adult feline liver organoid cultures for disease modeling of hepatic steatosis","volume":"8","author":"Kruitwagen","year":"2017","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib80","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1038\/s41422-019-0242-8","article-title":"Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury","volume":"29","author":"Wang","year":"2019","journal-title":"Cell Res."},{"key":"10.1016\/j.canlet.2022.216048_bib81","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/s41575-019-0181-3","article-title":"Organoid modelling of NAFLD","volume":"16","author":"Thomas","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib82","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.ebiom.2018.08.014","article-title":"Recapitulation of hepatitis B virus\u2013host interactions in liver organoids from human induced pluripotent stem cells","volume":"35","author":"Nie","year":"2018","journal-title":"EBioMedicine"},{"key":"10.1016\/j.canlet.2022.216048_bib83","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.chom.2018.02.005","article-title":"Single particle imaging of polarized hepatoma organoids upon hepatitis C virus infection reveals an ordered and sequential entry process","volume":"23","author":"Baktash","year":"2018","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.canlet.2022.216048_bib84","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.stem.2018.05.027","article-title":"Generation of hepatic stellate cells from human pluripotent stem cells enables in vitro modeling of liver fibrosis","volume":"23","author":"Coll","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib85","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1007\/s00125-016-3902-y","volume":"59","year":"2016","journal-title":"Diabetologia"},{"key":"10.1016\/j.canlet.2022.216048_bib86","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3109\/13813455.2012.683442","article-title":"The human hepatocyte cell lines IHH and HepaRG: models to study glucose, lipid and lipoprotein metabolism","volume":"118","author":"Samanez","year":"2012","journal-title":"Arch. Physiol. Biochem."},{"key":"10.1016\/j.canlet.2022.216048_bib87","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/nprot.2012.153","article-title":"Production of hepatocyte-like cells from human pluripotent stem cells","volume":"8","author":"Hannan","year":"2013","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2022.216048_bib88","article-title":"Novel defatting strategies reduce lipid accumulation in primary human culture models of liver steatosis","volume":"13","author":"Aoudjehane","year":"2020","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.canlet.2022.216048_bib89","first-page":"e6","article-title":"Recent advances in liver organoids and their use in in vitro modeling of non-alcoholic fatty liver disease","volume":"2","author":"Chi","year":"2022","journal-title":"Organ"},{"key":"10.1016\/j.canlet.2022.216048_bib90","doi-asserted-by":"crossref","first-page":"5734","DOI":"10.1021\/acsbiomaterials.0c00682","article-title":"Modeling human nonalcoholic fatty liver disease (NAFLD) with an organoids-on-a-chip system","volume":"6","author":"Wang","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.canlet.2022.216048_bib91","first-page":"85","article-title":"Metabolic studies in organoids: current applications, opportunities and challenges","volume":"1","author":"Richiardone","year":"2022","journal-title":"Organ"},{"key":"10.1016\/j.canlet.2022.216048_bib92","article-title":"Three-dimensional organoids as a model to study nonalcoholic fatty liver disease","author":"Hou","year":"2022","journal-title":"SSRN Electron. J."},{"key":"10.1016\/j.canlet.2022.216048_bib93","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.150328","article-title":"Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids","volume":"806","author":"Cheng","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.canlet.2022.216048_bib94","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jhep.2020.03.039","article-title":"A new definition for metabolic dysfunction-associated fatty liver disease: an international expert consensus statement","volume":"73","author":"Eslam","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib95","article-title":"Advancements in MAFLD modeling with human cell and organoid models","volume":"23","author":"Wang","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib96","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1136\/gutjnl-2019-319004","article-title":"Hepatitis E virus replication in human intestinal cells","volume":"69","author":"Marion","year":"2020","journal-title":"Gut"},{"key":"10.1016\/j.canlet.2022.216048_bib97","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3390\/v8090261","article-title":"Cell culture models for the investigation of hepatitis B and D virus infection","volume":"8","author":"Verrier","year":"2016","journal-title":"Viruses"},{"key":"10.1016\/j.canlet.2022.216048_bib98","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.3390\/v13081532","article-title":"Cell culture models for the study of hepatitis D virus entry and infection","volume":"13","author":"Heuschkel","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.canlet.2022.216048_bib99","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s13578-022-00890-8","article-title":"Liver organoids: an in vitro 3D model for liver cancer study","volume":"12","author":"Dong","year":"2022","journal-title":"Cell Biosci."},{"key":"10.1016\/j.canlet.2022.216048_bib100","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.60747","article-title":"Application of human liver organoids as a patient-derived primary model for HBV infection and related hepatocellular carcinoma","volume":"10","author":"De Crignis","year":"2021","journal-title":"Elife"},{"key":"10.1016\/j.canlet.2022.216048_bib101","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3390\/v13020211","article-title":"Three-dimensional cell culture systems for studying hepatitis C virus","volume":"13","author":"So","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.canlet.2022.216048_bib102","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abd3233","article-title":"Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice","volume":"6","author":"Brown","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.canlet.2022.216048_bib103","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.jcis.2021.02.067","article-title":"Investigating virus\u2013host cell interactions: comparative binding forces between hepatitis C virus-like particles and host cell receptors in 2D and 3D cell culture models","volume":"592","author":"Collett","year":"2021","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib104","doi-asserted-by":"crossref","unstructured":"V. Natarajan, C.R. Simoneau, A.L. Erickson, N.L. Meyers, J.L. Baron, S. Cooper, T.C. McDevitt, M. Ott, Modelling T-cell immunity against hepatitis C virus with liver organoids in a microfluidic coculture system, Open Biol. 12 (n.d.) 210320. https:\/\/doi.org\/10.1098\/rsob.210320.","DOI":"10.1098\/rsob.210320"},{"key":"10.1016\/j.canlet.2022.216048_bib105","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2021.103689","article-title":"Biliary Atresia \u2013 emerging diagnostic and therapy opportunities","volume":"74","author":"Lendahl","year":"2021","journal-title":"EBioMedicine"},{"key":"10.1016\/j.canlet.2022.216048_bib106","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/j.jhep.2020.06.012","article-title":"Beta-amyloid deposition around hepatic bile ducts is a novel pathobiological and diagnostic feature of biliary atresia","volume":"73","author":"Babu","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib107","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/MPG.0000000000002187","article-title":"Biliary atresia relevant human induced pluripotent stem cells recapitulate Key disease features in a dish","volume":"68","author":"Tian","year":"2019","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"10.1016\/j.canlet.2022.216048_bib108","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/hep.31653","article-title":"Organoids and spheroids as models for studying cholestatic liver injury and cholangiocarcinoma","volume":"74","author":"Sato","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2022.216048_bib109","doi-asserted-by":"crossref","first-page":"e01968","DOI":"10.1128\/mBio.01968-20","article-title":"Rotavirus infection and cytopathogenesis in human biliary organoids potentially recapitulate biliary atresia development","volume":"11","author":"Chen","year":"2020","journal-title":"mBio"},{"key":"10.1016\/j.canlet.2022.216048_bib110","article-title":"A bile duct\u2010on\u2010a\u2010chip with organ\u2010level functions - Du","year":"2020","journal-title":"Hepatology - Wiley Online Library"},{"key":"10.1016\/j.canlet.2022.216048_bib111","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1146\/annurev-pathol-011110-130246","article-title":"Pathogenesis of liver fibrosis","volume":"6","author":"Hernandez-Gea","year":"2011","journal-title":"Annu. Rev. Pathol."},{"key":"10.1016\/j.canlet.2022.216048_bib112","doi-asserted-by":"crossref","first-page":"835","DOI":"10.3390\/biology10090835","article-title":"Liver organoids: updates on disease modeling and biomedical applications","volume":"10","author":"Caiazza","year":"2021","journal-title":"Biology"},{"key":"10.1016\/j.canlet.2022.216048_bib113","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s12885-018-4459-6","article-title":"PDXliver: a database of liver cancer patient derived xenograft mouse models","volume":"18","author":"He","year":"2018","journal-title":"BMC Cancer"},{"key":"10.1016\/j.canlet.2022.216048_bib114","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.stem.2019.04.017","article-title":"Probing the tumor suppressor function of BAP1 in CRISPR-engineered human liver organoids","volume":"24","author":"Artegiani","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib115","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jhep.2019.03.013","article-title":"Global trends in mortality from intrahepatic and extrahepatic cholangiocarcinoma","volume":"71","author":"Bertuccio","year":"2019","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2022.216048_bib116","first-page":"31","article-title":"Application and research progress of organoids in cholangiocarcinoma and gallbladder carcinoma","volume":"11","author":"Huang","year":"2021","journal-title":"Am. J. Cancer Res."},{"key":"10.1016\/j.canlet.2022.216048_bib117","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1053\/j.gastro.2017.10.043","article-title":"MIR21 drives resistance to Heat Shock protein 90 inhibition in cholangiocarcinoma","volume":"154","author":"Lampis","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2022.216048_bib119","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/hep4.1312","article-title":"Murine liver organoids as a genetically flexible system to study liver cancer in vivo and in vitro","volume":"3","author":"Saborowski","year":"2019","journal-title":"Hepatol. Commun."},{"key":"10.1016\/j.canlet.2022.216048_bib120","doi-asserted-by":"crossref","first-page":"832","DOI":"10.3390\/cells9040832","article-title":"Cholangiocarcinoma disease modelling through patients derived organoids","volume":"9","author":"Amato","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.canlet.2022.216048_bib121","doi-asserted-by":"crossref","first-page":"8675","DOI":"10.3390\/ijms22168675","article-title":"Patient-derived organoids of cholangiocarcinoma","volume":"22","author":"Maier","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.canlet.2022.216048_bib122","doi-asserted-by":"crossref","first-page":"3613","DOI":"10.3390\/cells11223613","article-title":"Label-free imaging analysis of patient-derived cholangiocarcinoma organoids after sorafenib treatment","volume":"11","author":"Koch","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.canlet.2022.216048_bib123","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/s41416-022-01839-x","article-title":"Modelling immune cytotoxicity for cholangiocarcinoma with tumour-derived organoids and effector T cells","volume":"127","author":"Zhou","year":"2022","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.canlet.2022.216048_bib124","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1002\/stem.2500","article-title":"Liver regenerative medicine: from hepatocyte transplantation to bioartificial livers and bioengineered grafts","volume":"35","author":"Nicolas","year":"2017","journal-title":"Stem Cells Dayt. Ohio."},{"key":"10.1016\/j.canlet.2022.216048_bib125","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1016\/j.cell.2018.11.012","article-title":"Tissue repair signals and in vitro culture: inflammatory cytokine TNF\u03b1 promotes the expansion of primary hepatocytes in 3D culture","volume":"175","author":"Peng","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib126","doi-asserted-by":"crossref","DOI":"10.1016\/j.tranon.2020.100840","article-title":"Drug screening model meets cancer organoid technology","volume":"13","author":"Liu","year":"2020","journal-title":"Transl. Oncol."},{"key":"10.1016\/j.canlet.2022.216048_bib127","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nature11003","article-title":"The Cancer Cell Line Encyclopedia enables predictive modeling of anticancer drug sensitivity","volume":"483","author":"Barretina","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib129","first-page":"1012","article-title":"Organ-on-a-chip platforms for accelerating the evaluation of nanomedicine","volume":"6","author":"Chen","year":"2020","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.canlet.2022.216048_bib130","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.1039\/C8LC00869H","article-title":"In situ differentiation and generation of functional liver organoids from human iPSCs in a 3D perfusable chip system","volume":"18","author":"Wang","year":"2018","journal-title":"Lab Chip"},{"key":"10.1016\/j.canlet.2022.216048_bib131","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1007\/s12272-022-01390-6","article-title":"Advances in liver organoids: model systems for liver disease","volume":"45","author":"Lee","year":"2022","journal-title":"Arch Pharm. Res. (Seoul)"},{"key":"10.1016\/j.canlet.2022.216048_bib132","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1136\/gutjnl-2019-319256","article-title":"Liver organoids: from basic research to therapeutic applications","volume":"68","author":"Prior","year":"2019","journal-title":"Gut"},{"key":"10.1016\/j.canlet.2022.216048_bib133","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/s41392-022-01194-6","article-title":"Applications of human organoids in the personalized treatment for digestive diseases","volume":"7","author":"Wang","year":"2022","journal-title":"Signal Transduct. Targeted Ther."},{"key":"10.1016\/j.canlet.2022.216048_bib134","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1126\/science.aao2774","article-title":"Patient-derived organoids model treatment response of metastatic gastrointestinal cancers","volume":"359","author":"Vlachogiannis","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.canlet.2022.216048_bib135","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1158\/1078-0432.CCR-20-4116","article-title":"Organoid sensitivity correlates with therapeutic response in patients with pancreatic cancer","volume":"28","author":"Grossman","year":"2022","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.canlet.2022.216048_bib136","article-title":"Patient-derived organoids as a predictive biomarker for treatment response in cancer patients","volume":"5","author":"Wensink","year":"2021","journal-title":"NPJ Precis. Oncol."},{"key":"10.1016\/j.canlet.2022.216048_bib137","doi-asserted-by":"crossref","DOI":"10.1016\/j.tranon.2021.101015","article-title":"3D Bioprinted cancer models: revolutionizing personalized cancer therapy","volume":"14","author":"Augustine","year":"2021","journal-title":"Transl. Oncol."},{"key":"10.1016\/j.canlet.2022.216048_bib138","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.taap.2013.01.012","article-title":"A long-term three dimensional liver co-culture system for improved prediction of clinically relevant drug-induced hepatotoxicity","volume":"268","author":"Kostadinova","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.canlet.2022.216048_bib139","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.stem.2016.10.007","article-title":"Conversion of terminally committed hepatocytes to culturable bipotent progenitor cells with regenerative capacity","volume":"20","author":"Katsuda","year":"2017","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2022.216048_bib140","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1053\/j.gastro.2020.10.002","article-title":"High-fidelity drug induced liver injury screen using human PSC-derived organoids","volume":"160","author":"Shinozawa","year":"2021","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2022.216048_bib141","first-page":"1118","article-title":"Development of a human cardiac organoid injury model reveals innate regenerative potential","volume":"144","author":"Voges","year":"2017","journal-title":"Dev. Camb. Engl."},{"key":"10.1016\/j.canlet.2022.216048_bib142","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nature15695","article-title":"Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis","volume":"526","author":"Takasato","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib143","article-title":"Organoids to model liver disease","volume":"3","author":"Nuciforo","year":"2021","journal-title":"JHEP Rep"},{"key":"10.1016\/j.canlet.2022.216048_bib144","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s12967-020-02407-8","article-title":"A novel patient-derived organoids-based xenografts model for preclinical drug response testing in patients with colorectal liver metastases","volume":"18","author":"Jian","year":"2020","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.canlet.2022.216048_bib145","first-page":"263","article-title":"Patient-derived organoids (PDOs) and PDO-derived xenografts (PDOXs): new opportunities in establishing faithful pre-clinical cancer models","volume":"2","author":"Wang","year":"2022","journal-title":"J. Natl. Cancer Cent."},{"key":"10.1016\/j.canlet.2022.216048_bib146","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/liv.14692","article-title":"The biological process of lysine\u2010tRNA charging is therapeutically targetable in liver cancer","volume":"41","author":"Zhang","year":"2021","journal-title":"Liver Int."},{"key":"10.1016\/j.canlet.2022.216048_bib147","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1038\/s41388-020-01492-9","article-title":"Molecular biological analysis of 5-FU-resistant gastric cancer organoids; KHDRBS3 contributes to the attainment of features of cancer stem cell","volume":"39","author":"Ukai","year":"2020","journal-title":"Oncogene"},{"key":"10.1016\/j.canlet.2022.216048_bib148","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.copbio.2020.05.002","article-title":"Organoid technology and applications in cancer immunotherapy and precision medicine","volume":"65","author":"Homicsko","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.canlet.2022.216048_bib149","unstructured":"Fast and efficient generation of knock-in human organoids using homology-independent CRISPR\u2013Cas9 precision genome editing | Nat. Cell Biol., (n.d.). https:\/\/www.nature.com\/articles\/s41556-020-0472-5 (accessed May 2, 2022)."},{"key":"10.1016\/j.canlet.2022.216048_bib150","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1007\/s00702-020-02198-8","article-title":"Do not keep it simple: recent advances in the generation of complex organoids","volume":"127","author":"W\u00f6rsd\u00f6rfer","year":"2020","journal-title":"J. Neural. Transm."},{"key":"10.1016\/j.canlet.2022.216048_bib152","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12938-020-0752-0","article-title":"Organ-on-a-chip: recent breakthroughs and future prospects","volume":"19","author":"Wu","year":"2020","journal-title":"Biomed. Eng. Online"},{"key":"10.1016\/j.canlet.2022.216048_bib154","doi-asserted-by":"crossref","first-page":"E676","DOI":"10.3390\/mi10100676","article-title":"Engineered liver-on-a-chip platform to mimic liver functions and its biomedical applications: a review","volume":"10","author":"Deng","year":"2019","journal-title":"Micromachines"},{"key":"10.1016\/j.canlet.2022.216048_bib156","first-page":"E2945","article-title":"The tumor-on-chip: recent advances in the development of microfluidic systems to recapitulate the physiology of solid tumors","volume":"12","author":"Trujillo-de Santiago","year":"2019","journal-title":"Mater. Basel Switz."},{"key":"10.1016\/j.canlet.2022.216048_bib158","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s42242-020-00074-8","article-title":"A hepatocellular carcinoma\u2013bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles","volume":"3","author":"Sharifi","year":"2020","journal-title":"Bio-Des. Manuf."},{"key":"10.1016\/j.canlet.2022.216048_bib160","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1039\/C6LC00450D","article-title":"One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology","volume":"16","author":"Lee","year":"2016","journal-title":"Lab Chip"},{"key":"10.1016\/j.canlet.2022.216048_bib161","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s13206-020-4201-8","article-title":"Recent advances in 3D bioprinted tumor microenvironment","volume":"14","author":"Bae","year":"2020","journal-title":"BioChip J"},{"key":"10.1016\/j.canlet.2022.216048_bib162","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1126\/science.aav9051","article-title":"3D bioprinting of collagen to rebuild components of the human heart","volume":"365","author":"Lee","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.canlet.2022.216048_bib163","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.actbio.2019.06.017","article-title":"3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels","volume":"95","author":"Mollica","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.canlet.2022.216048_bib164","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1186\/s12943-015-0415-0","article-title":"Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling","volume":"14","author":"Sandercock","year":"2015","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.canlet.2022.216048_bib165","doi-asserted-by":"crossref","first-page":"7466","DOI":"10.1038\/s41598-019-43922-z","article-title":"A 3D bioprinter platform for mechanistic analysis of tumoroids and chimeric mammary organoids","volume":"9","author":"Reid","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib166","doi-asserted-by":"crossref","first-page":"E208","DOI":"10.3390\/mi11020208","article-title":"Immersion bioprinting of tumor organoids in multi-well plates for increasing chemotherapy screening throughput","volume":"11","author":"Maloney","year":"2020","journal-title":"Micromachines"},{"key":"10.1016\/j.canlet.2022.216048_bib167","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ygyno.2019.05.005","article-title":"Efficient use of patient-derived organoids as a preclinical model for gynecologic tumors","volume":"154","author":"Maru","year":"2019","journal-title":"Gynecol. Oncol."},{"key":"10.1016\/j.canlet.2022.216048_bib168","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1016\/j.celrep.2017.11.005","article-title":"Massive and reproducible production of liver buds entirely from human pluripotent stem cells","volume":"21","author":"Takebe","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.canlet.2022.216048_bib169","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/nature20168","article-title":"Designer matrices for intestinal stem cell and organoid culture","volume":"539","author":"Gjorevski","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib170","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.coi.2017.07.012","article-title":"Organoid culture systems to study host\u2013pathogen interactions","volume":"48","author":"Dutta","year":"2017","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.canlet.2022.216048_bib171","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/s41586-019-1289-x","article-title":"Individual brain organoids reproducibly form cell diversity of the human cerebral cortex","volume":"570","author":"Velasco","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2022.216048_bib172","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.3390\/cancers13153651","article-title":"Experimental models of hepatocellular carcinoma\u2014a preclinical perspective","volume":"13","author":"Blidisel","year":"2021","journal-title":"Cancers"}],"container-title":["Cancer Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383522005353?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383522005353?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T16:08:58Z","timestamp":1759594138000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304383522005353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2]]},"references-count":164,"alternative-id":["S0304383522005353"],"URL":"https:\/\/doi.org\/10.1016\/j.canlet.2022.216048","relation":{},"ISSN":["0304-3835"],"issn-type":[{"value":"0304-3835","type":"print"}],"subject":[],"published":{"date-parts":[[2023,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy","name":"articletitle","label":"Article Title"},{"value":"Cancer Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.canlet.2022.216048","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"216048"}}