{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:27:25Z","timestamp":1781886445630,"version":"3.54.5"},"reference-count":98,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["20230484454"],"award-info":[{"award-number":["20230484454"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1103400"],"award-info":[{"award-number":["2022YFA1103400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2604400"],"award-info":[{"award-number":["2023YFC2604400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["9235930035"],"award-info":[{"award-number":["9235930035"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cancer Letters"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.canlet.2025.217619","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T03:30:32Z","timestamp":1741577432000},"page":"217619","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Innovative construction and application of bile duct organoids: Unraveling the complexity of bile duct diseases and potential therapeutic strategies"],"prefix":"10.1016","volume":"618","author":[{"given":"Yadi","family":"Geng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziye","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianzi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siming","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.canlet.2025.217619_bib1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1053\/j.gastro.2009.03.035","article-title":"Mechanisms of liver development: concepts for understanding liver disorders and design of novel therapies","volume":"137","author":"Lemaigre","year":"2009","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2025.217619_bib2","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.bbadis.2017.08.021","article-title":"Bile acid receptors in the biliary tree: TGR5 in physiology and disease","volume":"1864","author":"Deutschmann","year":"2018","journal-title":"Biochim. Biophys. Acta, Mol. Basis Dis."},{"key":"10.1016\/j.canlet.2025.217619_bib3","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/s41575-019-0125-y","article-title":"Cholangiocyte pathobiology","volume":"16","author":"Banales","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib4","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1053\/j.gastro.2013.10.013","article-title":"Pathogenesis, diagnosis, and management of cholangiocarcinoma","volume":"145","author":"Ilyas","year":"2013","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2025.217619_bib5","article-title":"Epidemiology of cholangiocarcinoma","author":"Qurashi","year":"2023","journal-title":"Eur. J. Surg. Oncol."},{"key":"10.1016\/j.canlet.2025.217619_bib6","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1002\/hep.30289","article-title":"Intrahepatic cholangiocarcinoma: Continuing challenges and translational advances","volume":"69","author":"Sirica","year":"2019","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2025.217619_bib7","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/hep.31653","article-title":"Organoids and spheroids as models for studying cholestatic liver injury and cholangiocarcinoma","volume":"74","author":"Sato","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2025.217619_bib8","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1002\/hep.27165","article-title":"Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells","volume":"60","author":"Dianat","year":"2014","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2025.217619_bib9","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.suc.2014.01.001","article-title":"Anatomy and embryology of the biliary tract","volume":"94","author":"Keplinger","year":"2014","journal-title":"Surg Clin North Am"},{"key":"10.1016\/j.canlet.2025.217619_bib10","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.jhep.2011.09.022","article-title":"Development of the bile ducts: essentials for the clinical hepatologist","volume":"56","author":"Strazzabosco","year":"2012","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib11","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.biocel.2010.06.020","article-title":"Molecular mechanisms of bile duct development","volume":"43","author":"Zong","year":"2011","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib12","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1111\/jgh.13738","article-title":"Diagnostic considerations for cholestatic liver disease","volume":"32","author":"Pollock","year":"2017","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib13","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1002\/eji.201344270","article-title":"Implications of genome-wide association studies in novel therapeutics in primary biliary cirrhosis","volume":"44","author":"Carbone","year":"2014","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.canlet.2025.217619_bib14","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1056\/NEJMra1806175","article-title":"Organoids - preclinical models of human disease","volume":"380","author":"Li","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.canlet.2025.217619_bib15","first-page":"394","article-title":"Group A rotaviruses produce extrahepatic biliary obstruction in orally inoculated newborn mice","volume":"33","author":"Riepenhoff-Talty","year":"1993","journal-title":"Pediatr. Res."},{"issue":"7","key":"10.1016\/j.canlet.2025.217619_bib16","first-page":"1754","article-title":"Research advances in animal models of cholestatic liver disease","volume":"39","author":"Qingqing Zhang","year":"2023","journal-title":"J Clin Hepatol"},{"key":"10.1016\/j.canlet.2025.217619_bib17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/978-1-4939-9420-5_17","article-title":"A rotavirus-induced mouse model to study biliary atresia and neonatal cholestasis","author":"Mohanty","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib18","doi-asserted-by":"crossref","first-page":"410","DOI":"10.2353\/ajpath.2006.050404","article-title":"Lithocholic acid feeding induces segmental bile duct obstruction and destructive cholangitis in mice","volume":"168","author":"Fickert","year":"2006","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.canlet.2025.217619_bib19","doi-asserted-by":"crossref","first-page":"525","DOI":"10.2353\/ajpath.2007.061133","article-title":"A new xenobiotic-induced mouse model of sclerosing cholangitis and biliary fibrosis","volume":"171","author":"Fickert","year":"2007","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.canlet.2025.217619_bib20","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nbt.3275","article-title":"Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation","volume":"33","author":"Sampaziotis","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.canlet.2025.217619_bib21","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.jhep.2018.12.028","article-title":"Generation of hepatobiliary organoids from human induced pluripotent stem cells","volume":"70","author":"Wu","year":"2019","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib22","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/s41586-019-1598-0","article-title":"Modelling human hepato-biliary-pancreatic organogenesis from the foregut-midgut boundary","volume":"574","author":"Koike","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.canlet.2025.217619_bib23","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1038\/nm.4360","article-title":"Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids","volume":"23","author":"Sampaziotis","year":"2017","journal-title":"Nat Med"},{"key":"10.1016\/j.canlet.2025.217619_bib24","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.aaz6964","article-title":"Cholangiocyte organoids can repair bile ducts after transplantation in the human liver","volume":"371","author":"Sampaziotis","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.canlet.2025.217619_bib25","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s13287-024-03692-6","article-title":"Cholangiocyte organoids to study drug-induced injury","volume":"15","author":"Wang","year":"2024","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.canlet.2025.217619_bib26","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/j.jhep.2020.06.012","article-title":"Beta-amyloid deposition around hepatic bile ducts is a novel pathobiological and diagnostic feature of biliary atresia","volume":"73","author":"Babu","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/hep.32107","article-title":"Biliary organoids uncover delayed epithelial development and barrier function in biliary atresia","volume":"75","author":"Amarachintha","year":"2022","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2025.217619_bib28","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1007\/s10620-024-08570-y","article-title":"Single-cell transcriptomic profiling of cholangiocyte organoids derived from bile ducts of primary sclerosing cholangitis patients","volume":"69","author":"Frank","year":"2024","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.canlet.2025.217619_bib29","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/j.ajpath.2023.05.005","article-title":"Development of scaffold-free three-dimensional cholangiocyte organoids to study the progression of primary sclerosing cholangitis","volume":"193","author":"Zhang","year":"2023","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.canlet.2025.217619_bib30","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/s41467-023-35896-4","article-title":"Integrative analysis of multiple genomic data from intrahepatic cholangiocarcinoma organoids enables tumor subtyping","volume":"14","author":"Lee","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.canlet.2025.217619_bib31","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.celrep.2019.03.088","article-title":"Establishment of patient-derived organoids and drug screening for biliary tract carcinoma","volume":"27","author":"Saito","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.canlet.2025.217619_bib32","article-title":"Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application","volume":"4","author":"Ren","year":"2023","journal-title":"Cell Rep Med"},{"key":"10.1016\/j.canlet.2025.217619_bib33","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.ccell.2024.03.004","article-title":"Pharmacogenomic profiling of intra-tumor heterogeneity using a large organoid biobank of liver cancer","volume":"42","author":"Yang","year":"2024","journal-title":"Cancer Cell"},{"key":"10.1016\/j.canlet.2025.217619_bib34","doi-asserted-by":"crossref","first-page":"e678","DOI":"10.1002\/ctm2.678","article-title":"Patient-derived organoids for personalized gallbladder cancer modelling and drug screening","volume":"12","author":"Yuan","year":"2022","journal-title":"Clin. Transl. Med."},{"key":"10.1016\/j.canlet.2025.217619_bib35","article-title":"Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions","volume":"5","author":"Zhu","year":"2024","journal-title":"Cell Rep Med"},{"key":"10.1016\/j.canlet.2025.217619_bib36","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1002\/hep.30470","article-title":"Bile-derived organoids from patients with primary sclerosing cholangitis recapitulate their inflammatory immune profile","volume":"70","author":"Soroka","year":"2019","journal-title":"Hepatology"},{"key":"10.1016\/j.canlet.2025.217619_bib37","article-title":"Human bile contains cholangiocyte organoid-initiating cells which expand as functional cholangiocytes in non-canonical Wnt stimulating conditions","volume":"8","author":"Roos","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib38","doi-asserted-by":"crossref","first-page":"e566","DOI":"10.1002\/ctm2.566","article-title":"Cholangiocyte organoids from human bile retain a local phenotype and can repopulate bile ducts in vitro","volume":"11","author":"Roos","year":"2021","journal-title":"Clin. Transl. Med."},{"key":"10.1016\/j.canlet.2025.217619_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2022.121473","article-title":"Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids","volume":"284","author":"Willemse","year":"2022","journal-title":"Biomaterials"},{"key":"10.1016\/j.canlet.2025.217619_bib40","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1038\/s42003-021-02910-8","article-title":"Towards organoid culture without Matrigel","volume":"4","author":"Kozlowski","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib41","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202207752","article-title":"Decellularized extracellular matrix for remodeling bioengineering organoid's microenvironment","volume":"19","author":"Zhu","year":"2023","journal-title":"Small"},{"key":"10.1016\/j.canlet.2025.217619_bib42","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s12929-024-01086-7","article-title":"Enhancing organoid culture: harnessing the potential of decellularized extracellular matrix hydrogels for mimicking microenvironments","volume":"31","author":"Li","year":"2024","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.canlet.2025.217619_bib43","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1002\/bit.27613","article-title":"Scaffolds obtained from decellularized human extrahepatic bile ducts support organoids to establish functional biliary tissue in a dish","volume":"118","author":"Willemse","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.canlet.2025.217619_bib44","article-title":"Chemically defined organoid culture system for cholangiocyte differentiation","volume":"13","author":"Wang","year":"2024","journal-title":"Adv Healthc Mater"},{"key":"10.1016\/j.canlet.2025.217619_bib45","first-page":"672","article-title":"Hormonally and chemically defined expansion conditions for organoids of biliary tree Stem Cells","volume":"41","author":"Zhang","year":"2024","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.canlet.2025.217619_bib46","first-page":"452","article-title":"Generation and metabolomic characterization of functional ductal organoids with biliary tree networks in decellularized liver scaffolds","volume":"26","author":"Chen","year":"2023","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.canlet.2025.217619_bib47","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/bs.mcb.2020.03.011","article-title":"Pluripotent stem cell-derived cholangiocytes and cholangiocyte organoids","volume":"159","author":"Luce","year":"2020","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib48","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1038\/nm.4438","article-title":"Human primary liver cancer-derived organoid cultures for disease modeling and drug screening","volume":"23","author":"Broutier","year":"2017","journal-title":"Nat Med"},{"key":"10.1016\/j.canlet.2025.217619_bib49","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-79082-8","article-title":"Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease","volume":"10","author":"Verstegen","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.canlet.2025.217619_bib50","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1038\/s41596-020-00441-w","article-title":"Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells","volume":"16","author":"Koike","year":"2021","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.canlet.2025.217619_bib51","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.stem.2022.04.011","article-title":"Human branching cholangiocyte organoids recapitulate functional bile duct formation","volume":"29","author":"Roos","year":"2022","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2025.217619_bib52","doi-asserted-by":"crossref","first-page":"7424","DOI":"10.1038\/s41467-024-51487-3","article-title":"Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel","volume":"15","author":"Carolina","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.canlet.2025.217619_bib53","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/nbt.3294","article-title":"Directed differentiation of cholangiocytes from human pluripotent stem cells","volume":"33","author":"Ogawa","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.canlet.2025.217619_bib54","article-title":"Dysregulated activation of hippo-YAP1 signaling induces oxidative stress and aberrant development of intrahepatic biliary cells in biliary atresia","volume":"105","author":"Xie","year":"2024","journal-title":"Lab. Invest."},{"key":"10.1016\/j.canlet.2025.217619_bib55","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.94954","article-title":"Human hepatic organoids for the analysis of human genetic diseases","volume":"2","author":"Guan","year":"2017","journal-title":"JCI Insight"},{"key":"10.1016\/j.canlet.2025.217619_bib56","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.jhep.2021.03.029","article-title":"Downregulation of TGR5 (GPBAR1) in biliary epithelial cells contributes to the pathogenesis of sclerosing cholangitis","volume":"75","author":"Reich","year":"2021","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib57","first-page":"207","volume":"vol. 6","author":"Yao","year":"2023"},{"key":"10.1016\/j.canlet.2025.217619_bib58","doi-asserted-by":"crossref","DOI":"10.1097\/HC9.0000000000000454","article-title":"IL-17 signaling in primary sclerosing cholangitis patient-derived organoids","volume":"8","author":"Garcia Moreno","year":"2024","journal-title":"Hepatology Communications"},{"key":"10.1016\/j.canlet.2025.217619_bib59","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1007\/s10620-024-08570-y","article-title":"Single-cell transcriptomic profiling of cholangiocyte organoids derived from bile ducts of primary sclerosing cholangitis patients","volume":"69","author":"Frank","year":"2024","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.canlet.2025.217619_bib60","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1007\/s13238-020-00718-6","article-title":"Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids","volume":"11","author":"Zhao","year":"2020","journal-title":"Protein Cell"},{"key":"10.1016\/j.canlet.2025.217619_bib61","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01968-20","article-title":"Rotavirus infection and cytopathogenesis in human biliary organoids potentially recapitulate biliary atresia development","volume":"11","author":"Chen","year":"2020","journal-title":"mBio"},{"key":"10.1016\/j.canlet.2025.217619_bib62","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1007\/s12072-021-10237-z","article-title":"Application of CRISPR-Cas9 based gene editing to study the pathogenesis of colon and liver cancer using organoids","volume":"15","author":"Ramakrishna","year":"2021","journal-title":"Hepatol Int"},{"key":"10.1016\/j.canlet.2025.217619_bib63","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/hep4.1312","article-title":"Murine liver organoids as a genetically flexible system to study liver cancer in vivo and in vitro","volume":"3","author":"Saborowski","year":"2019","journal-title":"Hepatol Commun"},{"key":"10.1016\/j.canlet.2025.217619_bib64","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.stem.2019.04.017","article-title":"Probing the tumor suppressor function of BAP1 in CRISPR-engineered human liver organoids","volume":"24","author":"Artegiani","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2025.217619_bib65","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2022.104431","article-title":"Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents","volume":"88","author":"Shi","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.canlet.2025.217619_bib66","doi-asserted-by":"crossref","DOI":"10.3389\/ti.2024.13212","article-title":"Extrahepatic bile duct organoids as a model to study ischemia\/reperfusion injury during liver transplantation","volume":"37","author":"Kreiner","year":"2024","journal-title":"Transpl. Int."},{"key":"10.1016\/j.canlet.2025.217619_bib67","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1055\/s-0036-1597818","article-title":"Regenerative medicine and the biliary tree","volume":"37","author":"De Assuncao","year":"2017","journal-title":"Semin. Liver Dis."},{"key":"10.1016\/j.canlet.2025.217619_bib68","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.jcmgh.2021.10.009","article-title":"Recapitulating cholangiopathy-associated necroptotic cell death in vitro using human cholangiocyte organoids","volume":"13","author":"Shi","year":"2022","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib69","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jconrel.2024.08.006","article-title":"ROS-responsive nanoparticle delivery of obeticholic acid mitigate primary sclerosing cholangitis","volume":"374","author":"Yao","year":"2024","journal-title":"J Control Release"},{"key":"10.1016\/j.canlet.2025.217619_bib70","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1053\/j.gastro.2017.10.043","article-title":"MIR21 drives resistance to heat shock protein 90 inhibition in cholangiocarcinoma","volume":"154","author":"Lampis","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2025.217619_bib71","doi-asserted-by":"crossref","DOI":"10.3390\/cells11223613","article-title":"Label-free imaging analysis of patient-derived cholangiocarcinoma organoids after sorafenib treatment","volume":"11","author":"Koch","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.canlet.2025.217619_bib72","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202306174","article-title":"Escherichia coli-induced cGLIS3-mediated stress granules activate the NF-kappaB pathway to promote intrahepatic cholangiocarcinoma progression","volume":"11","author":"Kang","year":"2024","journal-title":"Adv. Sci."},{"key":"10.1016\/j.canlet.2025.217619_bib73","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.stem.2023.05.012","article-title":"Organoid cultures for cancer modeling","volume":"30","author":"Yan","year":"2023","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.canlet.2025.217619_bib74","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.adg3358","article-title":"Pharmaco-proteogenomic characterization of liver cancer organoids for precision oncology","volume":"15","author":"Ji","year":"2023","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.canlet.2025.217619_bib75","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/s41416-022-01839-x","article-title":"Modelling immune cytotoxicity for cholangiocarcinoma with tumour-derived organoids and effector T cells","volume":"127","author":"Zhou","year":"2022","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.canlet.2025.217619_bib76","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1002\/cac2.12452","article-title":"Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins","volume":"43","author":"Qiao","year":"2023","journal-title":"Cancer Commun."},{"key":"10.1016\/j.canlet.2025.217619_bib77","first-page":"11","article-title":"Scalable production of size-controlled cholangiocyte and cholangiocarcinoma organoids within liver extracellular matrix-containing microcapsules","author":"van Tienderen","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.canlet.2025.217619_bib78","article-title":"Continuous production of highly functional vascularized hepatobiliary organoids from human pluripotent stem cells using a scalable microfluidic platform","volume":"33","author":"Saeed Abbasalizadeh","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.canlet.2025.217619_bib79","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s42242-022-00213-3","article-title":"Facile suspension culture protocol of the liver biliary organoids","volume":"6","author":"Chen","year":"2023","journal-title":"Bio-des. Manuf."},{"key":"10.1016\/j.canlet.2025.217619_bib80","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s42242-022-00229-9","article-title":"3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses","volume":"6","author":"Qiong Liu","year":"2023","journal-title":"Bio-Design and Manufacturing"},{"key":"10.1016\/j.canlet.2025.217619_bib81","article-title":"Human vascularized bile duct-on-a chip: a multi-cellular micro-physiological system for studying cholestatic liver disease","volume":"16","author":"Du","year":"2023","journal-title":"Biofabrication"},{"key":"10.1016\/j.canlet.2025.217619_bib82","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202302912","article-title":"Bioengineered tubular biliary organoids","volume":"13","author":"Elci","year":"2024","journal-title":"Adv Healthc Mater"},{"key":"10.1016\/j.canlet.2025.217619_bib83","article-title":"High-throughput formation of pre-vascularized hiPSC-derived hepatobiliary organoids on a chip via nonparenchymal cell grafting","author":"Fan","year":"2025","journal-title":"Adv. Sci."},{"key":"10.1016\/j.canlet.2025.217619_bib84","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/s42003-024-05966-4","article-title":"OrgaSegment: deep-learning based organoid segmentation to quantify CFTR dependent fluid secretion","volume":"7","author":"Lefferts","year":"2024","journal-title":"Commun. Biol."},{"key":"10.1016\/j.canlet.2025.217619_bib85","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/s41597-023-02482-8","article-title":"Label-free tumor cells classification using deep learning and high-content imaging","volume":"10","author":"Piansaddhayanon","year":"2023","journal-title":"Sci. Data"},{"key":"10.1016\/j.canlet.2025.217619_bib86","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.gendis.2023.02.052","article-title":"Cancer organoids: a platform in basic and translational research","volume":"11","author":"Ma","year":"2024","journal-title":"Genes Dis"},{"key":"10.1016\/j.canlet.2025.217619_bib87","doi-asserted-by":"crossref","DOI":"10.3390\/biom14070794","article-title":"Human hepatobiliary organoids: recent advances in drug toxicity verification and drug screening","volume":"14","author":"Fang","year":"2024","journal-title":"Biomolecules"},{"key":"10.1016\/j.canlet.2025.217619_bib88","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1053\/j.gastro.2004.04.009","article-title":"Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 (Abcb4) knockout mice","volume":"127","author":"Fickert","year":"2004","journal-title":"Gastroenterology"},{"key":"10.1016\/j.canlet.2025.217619_bib89","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0168-8278(95)80432-3","article-title":"Induction of chronic cholangitis in the rat by trinitrobenzenesulfonic acid","volume":"22","author":"Mourelle","year":"1995","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib90","first-page":"13","article-title":"Morphologic and functional changes in the livers of rats after ligation or excision of the common bile duct","volume":"33","author":"Symeonidis","year":"1957","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.canlet.2025.217619_bib91","doi-asserted-by":"crossref","first-page":"286ra267","DOI":"10.1126\/scitranslmed.aaa1652","article-title":"Identification of a plant isoflavonoid that causes biliary atresia","volume":"7","author":"Lorent","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.canlet.2025.217619_bib92","first-page":"4634","article-title":"Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini-infected Syrian golden hamsters","volume":"38","author":"Thamavit","year":"1978","journal-title":"Cancer Res."},{"key":"10.1016\/j.canlet.2025.217619_bib93","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/carcin\/bgg053","article-title":"Prevention of dual promoting effects of pentachlorophenol, an environmental pollutant, on diethylnitrosamine-induced hepato- and cholangiocarcinogenesis in mice by green tea infusion","volume":"24","author":"Umemura","year":"2003","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.canlet.2025.217619_bib94","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0232-1513(84)80063-8","article-title":"Histogenesis of cholangiomas and cholangiocarcinomas in thioacetamide fed rats","volume":"26","author":"Praet","year":"1984","journal-title":"Exp Pathol"},{"key":"10.1016\/j.canlet.2025.217619_bib95","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1177\/019262339101900401","article-title":"Furan-induced hepatic cholangiocarcinomas in Fischer 344 rats","volume":"19","author":"Maronpot","year":"1991","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.canlet.2025.217619_bib96","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1093\/carcin\/14.11.2415","article-title":"Promotion of cholangiocarcinogenesis in the hamster liver by bile duct ligation after dimethylnitrosamine initiation","volume":"14","author":"Thamavit","year":"1993","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.canlet.2025.217619_bib97","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.jhep.2005.10.030","article-title":"Over-expression of interleukin-6 enhances cell survival and transformed cell growth in human malignant cholangiocytes","volume":"44","author":"Meng","year":"2006","journal-title":"J. Hepatol."},{"key":"10.1016\/j.canlet.2025.217619_bib98","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1002\/hep.24591","article-title":"MicroRNA down-regulated in human cholangiocarcinoma control cell cycle through multiple targets involved in the G1\/S checkpoint","volume":"54","author":"Olaru","year":"2011","journal-title":"Hepatology"}],"container-title":["Cancer Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383525001831?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304383525001831?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:06:44Z","timestamp":1777550804000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304383525001831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":98,"alternative-id":["S0304383525001831"],"URL":"https:\/\/doi.org\/10.1016\/j.canlet.2025.217619","relation":{},"ISSN":["0304-3835"],"issn-type":[{"value":"0304-3835","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Innovative construction and application of bile duct organoids: Unraveling the complexity of bile duct diseases and potential therapeutic strategies","name":"articletitle","label":"Article Title"},{"value":"Cancer Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.canlet.2025.217619","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"217619"}}