{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T14:56:57Z","timestamp":1794322617910,"version":"4.9.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100010954","name":"Qingdao National Laboratory for Marine Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010954","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017055","name":"NSFC-Shandong Joint Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Carbohydrate Polymers"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.carbpol.2020.117529","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T17:23:09Z","timestamp":1608225789000},"page":"117529","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"special_numbering":"C","title":["Structural characterization and effect on leukopenia of fucoidan from Durvillaea antarctica"],"prefix":"10.1016","volume":"256","author":[{"given":"Yingjie","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.carbpol.2020.117529_bib0005","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.carbpol.2008.03.004","article-title":"Preparation, partial characterization and bioactivity of exopolysaccharides from Lactobacillus casei LC2W","volume":"74","author":"Ai","year":"2008","journal-title":"Carbohydrate Polymers"},{"issue":"10","key":"10.1016\/j.carbpol.2020.117529_bib0010","doi-asserted-by":"crossref","DOI":"10.3390\/md15100301","article-title":"Fucoidan and fucosylated chondroitin sulfate stimulate hematopoiesis in cyclophosphamide-induced mice","volume":"15","author":"Anisimova","year":"2017","journal-title":"Marine Drugs"},{"issue":"14","key":"10.1016\/j.carbpol.2020.117529_bib0015","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1016\/j.carres.2010.07.009","article-title":"Further studies on the composition and structure of a fucoidan preparation from the brown alga Saccharina latissima","volume":"345","author":"Bilan","year":"2010","journal-title":"Carbohydrate Research"},{"issue":"2","key":"10.1016\/j.carbpol.2020.117529_bib0020","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.carres.2005.11.009","article-title":"Structure of a fucoidan from the brown seaweed Fucus serratus L","volume":"341","author":"Bilan","year":"2006","journal-title":"Carbohydrate Research"},{"issue":"8","key":"10.1016\/j.carbpol.2020.117529_bib0025","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0008-6215(02)00053-8","article-title":"Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag","volume":"337","author":"Bilan","year":"2002","journal-title":"Carbohydrate Research"},{"issue":"2","key":"10.1016\/j.carbpol.2020.117529_bib0030","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/0003-2697(73)90377-1","article-title":"New method for quantitative determination of uronic acids","volume":"54","author":"Blumenkrantz","year":"1973","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/j.carbpol.2020.117529_bib0035","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Analytical Biochemistry"},{"issue":"2","key":"10.1016\/j.carbpol.2020.117529_bib0040","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.carbpol.2012.12.047","article-title":"Structural elucidation of an extracellular polysaccharide produced by the marine fungus Aspergillus versicolor","volume":"93","author":"Chen","year":"2013","journal-title":"Carbohydrate Polymers"},{"key":"10.1016\/j.carbpol.2020.117529_bib0045","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.carbpol.2015.09.087","article-title":"Structural characterization and effect on anti-angiogenic activity of a fucoidan from Sargassum fusiforme","volume":"136","author":"Cong","year":"2016","journal-title":"Carbohydrate Polymers"},{"issue":"12","key":"10.1016\/j.carbpol.2020.117529_bib0050","doi-asserted-by":"crossref","first-page":"5014","DOI":"10.1158\/0008-5472.CAN-10-0100","article-title":"Reversal of chemotherapy-induced leukopenia using granulocyte macrophage colony-stimulating factor promotes bone metastasis that can be blocked with osteoclast inhibitors","volume":"70","author":"Dai","year":"2010","journal-title":"Cancer Research"},{"issue":"5","key":"10.1016\/j.carbpol.2020.117529_bib0055","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/jphp.12024","article-title":"Anti-inflammatory effect of a sulphated polysaccharide fraction extracted from the red algae Hypnea musciformis via the suppression of neutrophil migration by the nitric oxide signalling pathway","volume":"65","author":"de Brito","year":"2013","journal-title":"The Journal of Pharmacy and Pharmacology"},{"issue":"11","key":"10.1016\/j.carbpol.2020.117529_bib0060","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.2165\/00003088-200544110-00003","article-title":"Clinical pharmacokinetics of cyclophosphamide","volume":"44","author":"de Jonge","year":"2005","journal-title":"Clinical Pharmacokinetics"},{"issue":"4 Suppl 2","key":"10.1016\/j.carbpol.2020.117529_bib0065","first-page":"5","article-title":"Clinical use of subcutaneous G-CSF or GM-CSF in malignancy","volume":"19","author":"Disis","year":"2005","journal-title":"Oncology (Williston Park)"},{"issue":"1","key":"10.1016\/j.carbpol.2020.117529_bib0070","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.carbpol.2012.07.075","article-title":"A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects","volume":"91","author":"Dore","year":"2013","journal-title":"Carbohydrate Polymers"},{"issue":"4","key":"10.1016\/j.carbpol.2020.117529_bib0075","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0008-6215(01)00149-5","article-title":"Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum","volume":"333","author":"Duarte","year":"2001","journal-title":"Carbohydrate Research"},{"issue":"4265","key":"10.1016\/j.carbpol.2020.117529_bib0080","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/168167a0","article-title":"A colorimetric method for the determination of sugars","volume":"168","author":"Dubois","year":"1951","journal-title":"Nature"},{"issue":"9","key":"10.1016\/j.carbpol.2020.117529_bib0085","doi-asserted-by":"crossref","first-page":"5920","DOI":"10.3390\/md13095920","article-title":"Therapies from fucoidan: An update","volume":"13","author":"Fitton","year":"2015","journal-title":"Marine Drugs"},{"issue":"4","key":"10.1016\/j.carbpol.2020.117529_bib0090","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.jgr.2018.07.009","article-title":"Studies of the effects and mechanisms of ginsenoside Re and Rk3 on myelosuppression induced by cyclophosphamide","volume":"43","author":"Han","year":"2019","journal-title":"Journal of Ginseng Research"},{"issue":"3","key":"10.1016\/j.carbpol.2020.117529_bib0095","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.biologicals.2011.04.002","article-title":"A comparative study of monosaccharide composition analysis as a carbohydrate test for biopharmaceuticals","volume":"39","author":"Harazono","year":"2011","journal-title":"Biologicals"},{"issue":"11","key":"10.1016\/j.carbpol.2020.117529_bib0100","doi-asserted-by":"crossref","first-page":"6963","DOI":"10.1073\/pnas.78.11.6963","article-title":"Murine bone marrow cell line producing colony-stimulating factor","volume":"78","author":"Harigaya","year":"1981","journal-title":"Proceedings of National Academy of Science of the United States of America"},{"issue":"1","key":"10.1016\/j.carbpol.2020.117529_bib0105","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0008-6215(84)85106-X","article-title":"An improved procedure for the methylation analysis of oligosaccharides and polysaccharides","volume":"127","author":"Harris","year":"1984","journal-title":"Carbohydrate Research"},{"issue":"4","key":"10.1016\/j.carbpol.2020.117529_bib0110","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.micinf.2012.12.004","article-title":"Anti-influenza A virus characteristics of a fucoidan from sporophyll of Undaria pinnatifida in mice with normal and compromised immunity","volume":"15","author":"Hayashi","year":"2013","journal-title":"Microbes and Infection"},{"issue":"12","key":"10.1016\/j.carbpol.2020.117529_bib0115","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.3390\/ijms17121988","article-title":"Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds","volume":"17","author":"He","year":"2016","journal-title":"International Journal of Molecular Sciences"},{"key":"10.1016\/j.carbpol.2020.117529_bib0120","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.ijbiomac.2020.01.256","article-title":"Fucoidan from Laminaria japonica exerts antitumor effects on angiogenesis and micrometastasis in triple-negative breast cancer cells","volume":"149","author":"Hsu","year":"2020","journal-title":"International Journal of Biological Macromolecules"},{"issue":"11","key":"10.1016\/j.carbpol.2020.117529_bib0125","doi-asserted-by":"crossref","first-page":"19342","DOI":"10.3390\/ijms151119342","article-title":"Processed Aloe vera Gel Ameliorates Cyclophosphamide-Induced Immunotoxicity","volume":"15","author":"Im","year":"2014","journal-title":"International Journal of Molecular Sciences"},{"key":"10.1016\/j.carbpol.2020.117529_bib0130","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.carbpol.2019.02.040","article-title":"Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand","volume":"212","author":"Koh","year":"2019","journal-title":"Carbohydrate Polymers"},{"issue":"4","key":"10.1016\/j.carbpol.2020.117529_bib0135","first-page":"282","article-title":"Managing thrombocytopenia associated with cancer chemotherapy","volume":"29","author":"Kuter","year":"2015","journal-title":"Oncology-New York"},{"issue":"8","key":"10.1016\/j.carbpol.2020.117529_bib0140","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.3390\/molecules13081671","article-title":"Fucoidan: Structure and bioactivity","volume":"13","author":"Li","year":"2008","journal-title":"Molecules"},{"key":"10.1016\/j.carbpol.2020.117529_bib0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2019.115767","article-title":"Structure characterization, antioxidant and immunoregulatory properties of a novel fucoidan from the sea cucumber Stichopus chloronotus","volume":"231","author":"Li","year":"2020","journal-title":"Carbohydrate Polymers"},{"key":"10.1016\/j.carbpol.2020.117529_bib0150","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/j.ijbiomac.2019.11.036","article-title":"Fucoidan from sea cucumber Holothuria polii: Structural elucidation and stimulation of hematopoietic activity","volume":"154","author":"Li","year":"2020","journal-title":"International Journal of Biological Macromolecules"},{"issue":"3","key":"10.1016\/j.carbpol.2020.117529_bib0155","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.tiv.2003.09.001","article-title":"Inhibition of CFU-E\/BFU-E and CFU-GM colony growth by cyclophosphamide, 5-fluorouracil and taxol: Development of a high-throughput in vitro method","volume":"18","author":"Malerba","year":"2004","journal-title":"Toxicology in Vitro"},{"issue":"15","key":"10.1016\/j.carbpol.2020.117529_bib0160","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1055\/s-0035-1557821","article-title":"Suppressive effect of dietary fucoidan on proinflammatory immune response and MMP-1 expression in UVB-irradiated mouse skin","volume":"81","author":"Maruyama","year":"2015","journal-title":"Planta Medica"},{"key":"10.1016\/j.carbpol.2020.117529_bib0165","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.molstruc.2009.01.019","article-title":"FTIR spectroscopic characterization of Cu(II) coordination compounds with exopolysaccharide pullulan and its derivatives","volume":"924","author":"Mitic","year":"2009","journal-title":"Journal of Molecular Structure"},{"issue":"2","key":"10.1016\/j.carbpol.2020.117529_bib0170","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.chroma.2006.05.074","article-title":"Ion chromatographic measurement of sulfide, methanethiolate, sulfite and sulfate in aqueous and air samples","volume":"1121","author":"Ohira","year":"2006","journal-title":"Journal of Chromatography A"},{"issue":"11","key":"10.1016\/j.carbpol.2020.117529_bib0175","first-page":"2879","article-title":"Effect of direct cell-to-cell interaction between the KM-102 clonal human marrow stromal cell line and the HL-60 myeloid leukemic cell line on the differentiation and proliferation of the HL-60 line","volume":"47","author":"Ohkawa","year":"1987","journal-title":"Cancer Research"},{"issue":"41","key":"10.1016\/j.carbpol.2020.117529_bib0180","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M114.572297","article-title":"Fucosylated chondroitin sulfates from the body wall of the sea cucumber Holothuria forskali","volume":"289","author":"Panagos","year":"2014","journal-title":"The Journal of Biological Chemistry"},{"issue":"3","key":"10.1016\/j.carbpol.2020.117529_bib0185","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.cpha.2019.02.010","article-title":"Diagnostics for white blood cell abnormalities: leukocytosis and leukopenia","volume":"4","author":"Parente","year":"2019","journal-title":"Physician Assistant Clinics"},{"key":"10.1016\/j.carbpol.2020.117529_bib0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2019.114993","article-title":"A beta-1,3\/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator","volume":"222","author":"Su","year":"2019","journal-title":"Carbohydrate Polymers"},{"key":"10.1016\/j.carbpol.2020.117529_bib0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2019.115487","article-title":"Structural characterization and antiviral activity of two fucoidans from the brown algae Sargassum henslowianum","volume":"229","author":"Sun","year":"2020","journal-title":"Carbohydrate Polymers"},{"issue":"6","key":"10.1016\/j.carbpol.2020.117529_bib0200","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1248\/cpb.55.899","article-title":"Facile discrimination of aldose enantiomers by reversed-phase HPLC","volume":"55","author":"Tanaka","year":"2007","journal-title":"Chemical & Pharmaceutical Bulletin"},{"issue":"4","key":"10.1016\/j.carbpol.2020.117529_bib0205","doi-asserted-by":"crossref","first-page":"391","DOI":"10.9758\/cpn.2016.14.4.391","article-title":"Efficacy of adenine in the treatment of leukopenia and neutropenia associated with an overdose of antipsychotics or discontinuation of Lithium carbonate administration: Three case studies","volume":"14","author":"Tomita","year":"2016","journal-title":"Clinical Psychopharmacology and Neuroscience"},{"key":"10.1016\/j.carbpol.2020.117529_bib0210","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.carbpol.2017.12.071","article-title":"Structural characteristics and anticancer activity in vitro of fucoidan from brown alga Padina boryana","volume":"184","author":"Usoltseva","year":"2018","journal-title":"Carbohydrate Polymers"},{"issue":"2","key":"10.1016\/j.carbpol.2020.117529_bib0215","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ijbiomac.2010.05.010","article-title":"Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica","volume":"47","author":"Wang","year":"2010","journal-title":"International Journal of Biological Macromolecules"},{"key":"10.1016\/j.carbpol.2020.117529_bib0220","article-title":"Protective effects of biscoclaurine alkaloids on leukopenia induced by (60)Co-gamma radiation","volume":"2020","author":"Wang","year":"2020","journal-title":"Evidence Based Complementary and Alternative Medicine"},{"key":"10.1016\/j.carbpol.2020.117529_bib0225","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.carbpol.2018.12.060","article-title":"Chain conformation and biological activities of hyperbranched fucoidan derived from brown algae and its desulfated derivative","volume":"208","author":"Wei","year":"2019","journal-title":"Carbohydrate Polymers"},{"key":"10.1016\/j.carbpol.2020.117529_bib0230","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.carbpol.2018.03.019","article-title":"A beta-glucan from Durvillaea Antarctica has immunomodulatory effects on RAW264.7 macrophages via toll-like receptor 4","volume":"191","author":"Yang","year":"2018","journal-title":"Carbohydrate Polymers"},{"issue":"9","key":"10.1016\/j.carbpol.2020.117529_bib0235","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1021\/acs.jafc.5b05610","article-title":"Structural characterization and immunomodulatory activity of a novel polysaccharide from Lepidium meyenii","volume":"64","author":"Zhang","year":"2016","journal-title":"Journal of Agricultultural and Food Chemistry"},{"issue":"7","key":"10.1016\/j.carbpol.2020.117529_bib0240","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1634\/stemcells.2004-0173","article-title":"Simultaneous injection of bone marrow cells and stromal cells into bone marrow accelerates hematopoiesis in vivo","volume":"22","author":"Zhang","year":"2004","journal-title":"Stem Cells"}],"container-title":["Carbohydrate Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0144861720317021?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0144861720317021?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:04:26Z","timestamp":1758931466000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0144861720317021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":48,"alternative-id":["S0144861720317021"],"URL":"https:\/\/doi.org\/10.1016\/j.carbpol.2020.117529","relation":{},"ISSN":["0144-8617"],"issn-type":[{"value":"0144-8617","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural characterization and effect on leukopenia of fucoidan from Durvillaea antarctica","name":"articletitle","label":"Article Title"},{"value":"Carbohydrate Polymers","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.carbpol.2020.117529","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"117529"}}