{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:55Z","timestamp":1778544055766,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011752","name":"Synchrotron Light Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011752","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004352","name":"Suranaree University of Technology","doi-asserted-by":"publisher","award":["RSA6280012"],"award-info":[{"award-number":["RSA6280012"]}],"id":[{"id":"10.13039\/501100004352","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemico-Biological Interactions"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.cbi.2020.109223","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T19:17:46Z","timestamp":1596914266000},"page":"109223","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Roles of a protease from Euphorbia resinifera latex in human anticoagulant and antithrombotic activities"],"prefix":"10.1016","volume":"329","author":[{"given":"Jaruwan","family":"Siritapetawee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panida","family":"Khunkaewla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanjana","family":"Thumanu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbi.2020.109223_bib1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1177\/1043454206298693","article-title":"Theories of blood coagulation","volume":"24","author":"Riddel","year":"2007","journal-title":"J. Pediatr. Oncol. Nurs."},{"key":"10.1016\/j.cbi.2020.109223_bib2","doi-asserted-by":"crossref","first-page":"6210","DOI":"10.1038\/s41598-018-24422-y","article-title":"Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica","volume":"8","author":"Gogoi","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cbi.2020.109223_bib3","doi-asserted-by":"crossref","first-page":"e349","DOI":"10.1016\/j.thromres.2010.08.003","article-title":"Purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium Paenibacillus polymyxa EJS-3","volume":"126","author":"Lu","year":"2010","journal-title":"Thromb. Res."},{"key":"10.1016\/j.cbi.2020.109223_bib4","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1016\/j.biochi.2013.01.023","article-title":"Thrombolytic, anticoagulant and antiplatelet activities of codiase, a bi-functional fibrinolytic enzyme from Codium fragile","volume":"95","author":"Choi","year":"2013","journal-title":"Biochimie"},{"key":"10.1016\/j.cbi.2020.109223_bib5","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1080\/13102818.2009.10817623","article-title":"Synthetic and natural peptides as antithrombotic agents\u2014a view on the current development","volume":"23","author":"Atanassov","year":"2009","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"10.1016\/j.cbi.2020.109223_bib6","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1046\/j.1365-2141.2002.03954.x","article-title":"Activation and inactivation of human factor X by proteases derived from Ficus carica","volume":"119","author":"Richter","year":"2002","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.cbi.2020.109223_bib7","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/j.biochi.2006.06.007","article-title":"Purification and characterization of a 34-kDa, heat stable glycoprotein from Synadenium grantii latex: action on human fibrinogen and fibrin clot","volume":"88","author":"Rajesh","year":"2006","journal-title":"Biochimie"},{"key":"10.1016\/j.cbi.2020.109223_bib8","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1007\/s11239-008-0290-2","article-title":"Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities","volume":"28","author":"Shivaprasad","year":"2009","journal-title":"J. Thromb. Thrombolysis"},{"key":"10.1016\/j.cbi.2020.109223_bib9","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.ijbiomac.2019.09.190","article-title":"Identification and characterization of a protease (EuRP-61) from Euphorbia resinifera latex","volume":"145","author":"Siritapetawee","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.cbi.2020.109223_bib10","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.cbi.2020.109223_bib11","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1093\/oxfordjournals.jbchem.a127720","article-title":"Studies on snake venom. XIII. Chromatographic separation and properties of three proteinases from Agkistrodon halys blomhoffii venom","volume":"53","author":"Satake","year":"1963","journal-title":"J. Biochem."},{"key":"10.1016\/j.cbi.2020.109223_bib12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0041-0101(69)90154-8","article-title":"Coagulant and anticoagulant action of snake venoms","volume":"7","author":"Denson","year":"1969","journal-title":"Toxicon"},{"key":"10.1016\/j.cbi.2020.109223_bib13","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1006\/abio.1994.1066","article-title":"A colorimetric method for the measurement of platelet adhesion in microtiter plates","volume":"216","author":"Bellavite","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cbi.2020.109223_bib14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1136\/jcp.4.1.37","article-title":"The normal platelet count in man","volume":"4","author":"Sloan","year":"1951","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.cbi.2020.109223_bib15","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ab.2006.06.037","article-title":"Monitoring modulators of platelet aggregation in a microtiter plate assay","volume":"357","author":"Moran","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cbi.2020.109223_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0088422","article-title":"Eutirucallin, a RIP-2 type lectin from the latex of Euphorbia tirucalli L. presents proinflammatory properties","volume":"9","author":"Santana","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.cbi.2020.109223_bib17","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/hon.2900070405","article-title":"In vitro chemosensitivity testing of leukemic cells: prediction of response to chemotherapy in patients with acute non-lymphocytic leukemia","volume":"7","author":"Santini","year":"1989","journal-title":"Hematol. Oncol."},{"key":"10.1016\/j.cbi.2020.109223_bib18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1042\/bse0380095","article-title":"Proteases in blood clotting","volume":"38","author":"Walsh","year":"2002","journal-title":"Essays Biochem."},{"key":"10.1016\/j.cbi.2020.109223_bib19","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/S0167-4838(99)00024-2","article-title":"Purification and characterization of a serine protease with fibrinolytic activity from Tenodera sinensis (praying mantis)","volume":"1430","author":"Hahn","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cbi.2020.109223_bib20","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1073\/pnas.66.3.738","article-title":"Subunit structure of human fibrinogen, soluble fibrin, and cross-linked insoluble fibrin","volume":"66","author":"McKee","year":"1970","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.cbi.2020.109223_bib21","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1055\/s-2007-981575","article-title":"Comparative study on plant latex proteases and their involvement in hemostasis: a special emphasis on clot inducing and dissolving properties","volume":"73","author":"Rajesh","year":"2007","journal-title":"Planta Med."},{"key":"10.1016\/j.cbi.2020.109223_bib22","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jep.2009.02.016","article-title":"Thrombin like activity of Asclepias curassavica L. latex: action of cysteine proteases","volume":"123","author":"Shivaprasad","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.cbi.2020.109223_bib23","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0167-4838(85)90076-7","article-title":"Specificity of hemorrhagic proteinase from Crotalus atrox (western diamondback rattlesnake) venom","volume":"829","author":"Komori","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cbi.2020.109223_bib24","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jep.2015.06.018","article-title":"Comparative analysis of procoagulant and fibrinogenolytic activity of crude protease fractions of turmeric species","volume":"172","author":"Shivalingu","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.cbi.2020.109223_bib25","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1093\/labmed\/lmx050","article-title":"Coagulation testing in the core laboratory","volume":"48","author":"Winter","year":"2017","journal-title":"Lab. Med."},{"key":"10.1016\/j.cbi.2020.109223_bib26","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jacc.2005.08.055","article-title":"Dependence of platelet thrombus stability on sustained glycoprotein IIb\/IIIa activation through adenosine 5'-diphosphate receptor stimulation and cyclic calcium signaling","volume":"47","author":"Goto","year":"2006","journal-title":"J. Am. Coll. Cardiol."},{"key":"10.1016\/j.cbi.2020.109223_bib27","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1172\/JCI12242","article-title":"Molecular identification and characterization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs","volume":"107","author":"Foster","year":"2001","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.cbi.2020.109223_bib28","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1002\/jrs.5540","article-title":"Analysis of platelets in hypertensive and normotensive individuals using Raman and Fourier transform infrared-attenuated total reflectance spectroscopies","volume":"50","author":"Garc\u00eda-Rubio","year":"2019","journal-title":"J. Raman Spectrosc."},{"key":"10.1016\/j.cbi.2020.109223_bib29","series-title":"Biological and Biomedical Infrared Spectroscopy","first-page":"272","article-title":"Biomedical FTIR spectroscopy of lipids","author":"Wolkers","year":"2009"},{"key":"10.1016\/j.cbi.2020.109223_bib30","doi-asserted-by":"crossref","first-page":"112099","DOI":"10.1016\/j.jep.2019.112099","article-title":"Characterization of active anticoagulant fraction and a fibrin(ogen)olytic serine protease from leaves of Clerodendrum colebrookianum, a traditional ethno-medicinal plant used to reduce hypertension","volume":"243","author":"Gogoi","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.cbi.2020.109223_bib31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.biochi.2016.03.015","article-title":"Fibrin(ogen)olytic and antiplatelet activities of a subtilisin-like protease from Solanum tuberosum (StSBTc-3)","volume":"125","author":"Pepe","year":"2016","journal-title":"Biochimie"},{"key":"10.1016\/j.cbi.2020.109223_bib32","doi-asserted-by":"crossref","first-page":"172875","DOI":"10.1016\/j.ejphar.2019.172875","article-title":"Activated clotting factor X mediates mitochondrial alterations and inflammatory responses via protease-activated receptor signaling in alveolar epithelial cells","volume":"869","author":"Bukowska","year":"2020","journal-title":"Eur. J. Pharmacol."}],"container-title":["Chemico-Biological Interactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009279720310334?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009279720310334?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T14:00:56Z","timestamp":1757858456000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009279720310334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":32,"alternative-id":["S0009279720310334"],"URL":"https:\/\/doi.org\/10.1016\/j.cbi.2020.109223","relation":{},"ISSN":["0009-2797"],"issn-type":[{"value":"0009-2797","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Roles of a protease from Euphorbia resinifera latex in human anticoagulant and antithrombotic activities","name":"articletitle","label":"Article Title"},{"value":"Chemico-Biological Interactions","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbi.2020.109223","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109223"}}