{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:44:06Z","timestamp":1791945846114,"version":"4.3.4"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22021003"],"award-info":[{"award-number":["22021003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["KK.01.1.1.02.0007"],"award-info":[{"award-number":["KK.01.1.1.02.0007"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["XDB0750300"],"award-info":[{"award-number":["XDB0750300"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemico-Biological Interactions"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.cbi.2025.111722","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T06:44:49Z","timestamp":1756968289000},"page":"111722","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Simplified assessment of acetylcholinesterase inhibition by environmental organophosphorus pesticides"],"prefix":"10.1016","volume":"421","author":[{"given":"Ruihong","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongchao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanglei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3937-3520","authenticated-orcid":false,"given":"Antonio","family":"Zandona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1301-6816","authenticated-orcid":false,"given":"Tena","family":"\u010cade\u017e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9863-886X","authenticated-orcid":false,"given":"Zrinka","family":"Kovarik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6308-5273","authenticated-orcid":false,"given":"Heidi Qunhui","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.cbi.2025.111722_bib1","doi-asserted-by":"crossref","first-page":"488","DOI":"10.2174\/092986708783503203","article-title":"Pathways of acetylcholine synthesis, transport and release as targets for treatment of adult-onset cognitive dysfunction","volume":"15","author":"Amenta","year":"2008","journal-title":"Curr. Med. Chem."},{"issue":"6","key":"10.1016\/j.cbi.2025.111722_bib2","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1046\/j.1460-9568.2003.02867.x","article-title":"Respiratory survival mechanisms in acetylcholinesterase knockout mouse","volume":"18","author":"Chatonnet","year":"2003","journal-title":"Eur. J. Neurosci."},{"issue":"2","key":"10.1016\/j.cbi.2025.111722_bib3","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.bbr.2009.12.046","article-title":"Cholinergic systems mediate action from movement to higher consciousness","volume":"221","author":"Woolf","year":"2011","journal-title":"Behav. Brain Res."},{"issue":"4","key":"10.1016\/j.cbi.2025.111722_bib4","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/jnc.15887","article-title":"Recent advances in cholinergic mechanisms as reactions to toxicity, stress, and neuroimmune insults","volume":"168","author":"Kovarik","year":"2024","journal-title":"J. Neurochem."},{"key":"10.1016\/j.cbi.2025.111722_bib5","article-title":"N-methyl-d-aspartate receptors: structure, function, and role in organophosphorus compound poisoning","author":"Kolic","year":"2024","journal-title":"Biofactors"},{"issue":"21","key":"10.1016\/j.cbi.2025.111722_bib6","doi-asserted-by":"crossref","first-page":"9055","DOI":"10.1021\/ac901714h","article-title":"Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples","volume":"81","author":"Hossain","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.cbi.2025.111722_bib7","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.tox.2012.12.007","article-title":"Protein adducts as biomarkers of exposure to organophosphorus compounds","volume":"307","author":"Marsillach","year":"2013","journal-title":"Toxicology"},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib8","first-page":"76","article-title":"Environmental exposure to glyphosate does not inhibit human acetylcholinesterase and butyrylcholinesterase","volume":"75","author":"Kolic","year":"2024","journal-title":"Arh. Hig. Rada. Toksikol."},{"issue":"4","key":"10.1016\/j.cbi.2025.111722_bib9","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1021\/acsabm.1c00069","article-title":"Oxidase-like nanozyme-mediated altering of the aspect ratio of gold nanorods for breaking through H(2)O(2)-Supported multicolor colorimetric assay: application in the detection of acetylcholinesterase activity and its inhibitors","volume":"4","author":"Fu","year":"2021","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.cbi.2025.111722_bib10","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-1-0716-2213-1_6","article-title":"Acetylcholinesterase inhibition assays for high-throughput screening","volume":"2474","author":"Li","year":"2022","journal-title":"Methods Mol. Biol."},{"issue":"8","key":"10.1016\/j.cbi.2025.111722_bib11","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1248\/cpb.c18-00944","article-title":"A probe for fluorescence detection of the acetylcholinesterase activity based on molecularly imprinted polymers coated carbon dots","volume":"67","author":"Jia","year":"2019","journal-title":"Chem. Pharm. Bull. (Tokyo)"},{"issue":"27","key":"10.1016\/j.cbi.2025.111722_bib12","doi-asserted-by":"crossref","first-page":"15105","DOI":"10.1039\/D0CP01647K","article-title":"Enzyme-based optical biosensors for organophosphate class of pesticide detection","volume":"22","author":"Kaur","year":"2020","journal-title":"Phys. Chem. Chem. Phys."},{"issue":"4","key":"10.1016\/j.cbi.2025.111722_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0231981","article-title":"Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides","volume":"15","author":"Wang","year":"2020","journal-title":"PLoS One"},{"issue":"1\u20133","key":"10.1016\/j.cbi.2025.111722_bib14","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cbi.2008.04.032","article-title":"Advantages of the WRAIR whole blood cholinesterase assay: comparative analysis to the micro-Ellman, test-mate ChE, and michel (DeltapH) assays","volume":"175","author":"Haigh","year":"2008","journal-title":"Chem. Biol. Interact."},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib15","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ab.2012.06.025","article-title":"Acetylcholinesterase inhibition-based biosensors for pesticide determination: a review","volume":"429","author":"Pundir","year":"2012","journal-title":"Anal. Biochem."},{"issue":"5","key":"10.1016\/j.cbi.2025.111722_bib16","doi-asserted-by":"crossref","DOI":"10.3390\/s20051395","article-title":"Detection of Escherichia coli O157:H7 using automated immunomagnetic separation and enzyme-based colorimetric assay","volume":"20","author":"Park","year":"2020","journal-title":"Sensors (Basel)"},{"key":"10.1016\/j.cbi.2025.111722_bib17","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib18","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1432-1033.1984.tb08079.x","article-title":"Kinetics for the inhibition of acetylcholinesterase from the electric eel by some organophosphates and carbamates","volume":"140","author":"Forsberg","year":"1984","journal-title":"Eur. J. Biochem."},{"issue":"9","key":"10.1016\/j.cbi.2025.111722_bib19","doi-asserted-by":"crossref","first-page":"10142","DOI":"10.3390\/molecules170910142","article-title":"Acetylcholinesterase-inhibiting activity of salicylanilide N-alkylcarbamates and their molecular docking","volume":"17","author":"Imramovsky","year":"2012","journal-title":"Molecules"},{"key":"10.1016\/j.cbi.2025.111722_bib20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.cbi.2015.01.006","article-title":"Acetylcholinesterase activity of electric eel is increased or decreased by selected monoterpenoids and phenylpropanoids in a concentration-dependent manner","volume":"229","author":"Lopez","year":"2015","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbi.2025.111722_bib21","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1146\/annurev.pa.34.040194.001433","article-title":"The cholinesterases: from genes to proteins","volume":"34","author":"Taylor","year":"1994","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.cbi.2025.111722_bib22","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.scitotenv.2012.09.058","article-title":"Comparative effect of pesticides on brain acetylcholinesterase in tropical fish","volume":"441","author":"Assis","year":"2012","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbi.2025.111722_bib23","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tiv.2019.01.002","article-title":"Use of high-throughput enzyme-based assay with xenobiotic metabolic capability to evaluate the inhibition of acetylcholinesterase activity by organophosphorous pesticides","volume":"56","author":"Li","year":"2019","journal-title":"Toxicol. Vitro"},{"issue":"4","key":"10.1016\/j.cbi.2025.111722_bib24","doi-asserted-by":"crossref","DOI":"10.1289\/EHP6993","article-title":"Profiling the Tox21 chemical collection for acetylcholinesterase inhibition","volume":"129","author":"Li","year":"2021","journal-title":"Environ. Health Perspect."},{"issue":"15","key":"10.1016\/j.cbi.2025.111722_bib25","doi-asserted-by":"crossref","first-page":"8065","DOI":"10.1021\/acs.est.7b04004","article-title":"Acetylcholinesterase is a potential biomarker for a broad spectrum of organic environmental pollutants","volume":"52","author":"Fu","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"9","key":"10.1016\/j.cbi.2025.111722_bib26","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1021\/acs.biochem.5c00024","article-title":"Protonation state insights into the influence of biocatalytic function for acetylcholinesterase mediated by neonicotinoids","volume":"64","author":"He","year":"2025","journal-title":"Biochemistry"},{"issue":"Pt 10 Pt 2","key":"10.1016\/j.cbi.2025.111722_bib27","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1107\/S0907444902011642","article-title":"Structure of a complex of the potent and specific inhibitor BW284C51 with Torpedo californica acetylcholinesterase","volume":"58","author":"Felder","year":"2002","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib28","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/sj.bjp.0705965","article-title":"The acetylcholinesterase inhibitor BW284c51 is a potent blocker of torpedo nicotinic AchRs incorporated into the xenopus oocyte membrane","volume":"144","author":"Olivera-Bravo","year":"2005","journal-title":"Br. J. Pharmacol."},{"issue":"10","key":"10.1016\/j.cbi.2025.111722_bib29","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1002\/j.1460-2075.1992.tb05439.x","article-title":"Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center","volume":"11","author":"Shafferman","year":"1992","journal-title":"EMBO J."},{"issue":"5","key":"10.1016\/j.cbi.2025.111722_bib30","doi-asserted-by":"crossref","first-page":"2926","DOI":"10.1021\/acs.est.7b06376","article-title":"Development and application of a novel bioassay system for dioxin determination and aryl hydrocarbon receptor activation evaluation in ambient-air samples","volume":"52","author":"Zhang","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.cbi.2025.111722_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2022.114796","article-title":"Comparing the effects of organic cosolvents on acetylcholinesterase and butyrylcholinesterase activity","volume":"654","author":"Novales","year":"2022","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-00953-9","article-title":"Assessment of four organophosphorus pesticides as inhibitors of human acetylcholinesterase and butyrylcholinesterase","volume":"11","author":"Cadez","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cbi.2025.111722_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2023.110506","article-title":"Selected herbicides screened for toxicity and analysed as inhibitors of both cholinesterases","volume":"379","author":"Pehar","year":"2023","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbi.2025.111722_bib34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1146\/annurev-pharmtox-010716-104926","article-title":"Organophosphorus xenobiotic toxicology","volume":"57","author":"Casida","year":"2017","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"issue":"1","key":"10.1016\/j.cbi.2025.111722_bib35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1289\/ehp.0900824","article-title":"Mass spectrometric analyses of organophosphate insecticide oxon protein adducts","volume":"118","author":"Thompson","year":"2010","journal-title":"Environ. Health Perspect."},{"issue":"10","key":"10.1016\/j.cbi.2025.111722_bib36","doi-asserted-by":"crossref","first-page":"8451","DOI":"10.1007\/s10661-013-3186-3","article-title":"Simultaneous analysis of endosulfan, chlorpyrifos, and their metabolites in natural soil and water samples using gas chromatography-tandem mass spectrometry","volume":"185","author":"Tiwari","year":"2013","journal-title":"Environ. Monit. Assess."}],"container-title":["Chemico-Biological Interactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009279725003527?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009279725003527?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T05:02:31Z","timestamp":1780722151000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009279725003527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":36,"alternative-id":["S0009279725003527"],"URL":"https:\/\/doi.org\/10.1016\/j.cbi.2025.111722","relation":{},"ISSN":["0009-2797"],"issn-type":[{"value":"0009-2797","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simplified assessment of acetylcholinesterase inhibition by environmental organophosphorus pesticides","name":"articletitle","label":"Article Title"},{"value":"Chemico-Biological Interactions","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbi.2025.111722","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"111722"}}