{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:18:30Z","timestamp":1790860710634,"version":"4.1.0"},"reference-count":30,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["PHD\/0216\/2546"],"award-info":[{"award-number":["PHD\/0216\/2546"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.cbpb.2007.07.004","type":"journal-article","created":{"date-parts":[[2007,7,17]],"date-time":"2007-07-17T07:22:42Z","timestamp":1184656962000},"page":"382-389","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":59,"title":["Trypsin from the pyloric caeca of bluefish (Pomatomus saltatrix)"],"prefix":"10.1016","volume":"148","author":[{"given":"Sappasith","family":"Klomklao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soottawat","family":"Benjakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonnop","family":"Visessanguan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Kishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin K.","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpb.2007.07.004_bib1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S1096-4959(03)00082-4","article-title":"Partial purification and characterization of trimethylamine-N-oxide demethylase from lizardfish kidney","volume":"135","author":"Benjakul","year":"2003","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1745-4514.2001.tb00734.x","article-title":"Partial purification and characterization of a thermostable trypsin from pyloric caeca of tambaqui (Colossoma macropomum)","volume":"25","author":"Bezerra","year":"2001","journal-title":"J. Food Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib3","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1016\/j.procbio.2004.06.066","article-title":"Alkaline proteinase from intestine of Nile tilapia (Oreochromis niloticus)","volume":"40","author":"Bezerra","year":"2005","journal-title":"Process Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib4","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1046\/j.1444-2906.2000.00185.x","article-title":"Purification and characterization of two anionic trypsins from the hepatopancreas of carp","volume":"66","author":"Cao","year":"2000","journal-title":"Fish. Sci."},{"key":"10.1016\/j.cbpb.2007.07.004_bib5","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.cbpc.2004.09.031","article-title":"Isolation and characterization of trypsin from pyloric caeca of Monterey sardine (Sardinops sagax caerulea)","volume":"140","author":"Castillo-Yanez","year":"2005","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib6","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1111\/j.1745-4514.2005.00019.x","article-title":"Purification and characterization of an alkaline protease from the viscera of bolti fish (Tilapia nilotica)","volume":"29","author":"El-Beltagy","year":"2005","journal-title":"J. Food Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib7","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0003-9861(61)90145-X","article-title":"The preparation and properties of two new chromogenic substrates of trypsin","volume":"95","author":"Erlanger","year":"1961","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.cbpb.2007.07.004_bib8","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1111\/j.1432-1033.1993.tb18341.x","article-title":"Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen","volume":"217","author":"Gudmundsdottir","year":"1993","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib9","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1021\/bi00741a002","article-title":"Determination of the amino sequence of porcine trypsin by sequenator analysis","volume":"12","author":"Hermodson","year":"1973","journal-title":"Biochemistry"},{"key":"10.1016\/j.cbpb.2007.07.004_bib10","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1139\/y59-157","article-title":"A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin","volume":"37","author":"Hummel","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"10.1016\/j.cbpb.2007.07.004_bib11","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S1096-4959(02)00062-3","article-title":"Isolation and characteristics of trypsin from the pyloric caeca of the starfish Asterina pectinifera","volume":"132","author":"Kishimura","year":"2002","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib12","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1745-4514.2005.00029.x","article-title":"Characteristics of two trypsin isozymes from the viscera of Japanese anchovy (Engraulis japonica)","volume":"29","author":"Kishimura","year":"2005","journal-title":"J. Food Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib13","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.foodchem.2005.03.008","article-title":"Characteristics of trypsins from the viscera of true sardine (Sardinops melanostictus) and the pyloric caeca of arabesque greenling (Pleuroprammus azonus)","volume":"97","author":"Kishimura","year":"2006","journal-title":"Food Chem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib14","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1745-4514.2004.05203.x","article-title":"Comparative studies on proteolytic activity of spleen extracts from three tuna species commonly used in Thailand","volume":"28","author":"Klomklao","year":"2004","journal-title":"J. Food Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib15","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1016\/j.procbio.2005.03.009","article-title":"Partitioning and recovery of proteinase from tuna spleen by aqueous two-phase systems","volume":"40","author":"Klomklao","year":"2005","journal-title":"Process Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib16","doi-asserted-by":"crossref","first-page":"5617","DOI":"10.1021\/jf060699d","article-title":"Purification and characterization of trypsin from the spleen of tongol tuna (Thunnus tonggol)","volume":"54","author":"Klomklao","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib17","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.cbpb.2006.01.006","article-title":"Trypsins from yellowfin tuna (Thunnus albacores) spleen: purification and characterization","volume":"144","author":"Klomklao","year":"2006","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib18","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.foodchem.2005.05.047","article-title":"Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen","volume":"98","author":"Klomklao","year":"2006","journal-title":"Food Chem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib19","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1016\/j.foodchem.2006.01.001","article-title":"Purification and characterization of trypsins from the spleen of skipjack tuna (Katsuwonus pelamis)","volume":"100","author":"Klomklao","year":"2007","journal-title":"Food Chem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib20","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1021\/bi00623a016","article-title":"Structure of bovine trypsinogen at 1.9\u00a0\u00c5 resolution","volume":"16","author":"Kossiakoff","year":"1977","journal-title":"Biochemistry"},{"key":"10.1016\/j.cbpb.2007.07.004_bib21","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.cbpb.2005.12.020","article-title":"Isolation and characterization of a trypsin fraction from the pyloric caeca of chinook salmon (Oncorhynchus tshawytscha)","volume":"143","author":"Kurtovic","year":"2006","journal-title":"Comp. Biochem, Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"277","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.cbpb.2007.07.004_bib23","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib24","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1111\/j.1432-1033.1995.677zz.x","article-title":"Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon","volume":"232","author":"Male","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.cbpb.2007.07.004_bib25","first-page":"2669","article-title":"Differential regulation of trypsinogen mRNA translation: full-length mRNA sequences encoding two oppositely charged trypsinogen isoenzymes in the dog pancreas","volume":"5","author":"Pinsky","year":"1985","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.cbpb.2007.07.004_bib26","series-title":"Seafood Enzymes: Utilization and Influence on Postharvest Seafood Quality","first-page":"531","article-title":"Digestive proteinases from marine animals","author":"Simpson","year":"2000"},{"key":"10.1016\/j.cbpb.2007.07.004_bib27","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/0305-0491(84)90375-4","article-title":"Trypsin from Greenland cod, Gadus ogac. Isolation and comparative properties","volume":"79","author":"Simpson","year":"1984","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2007.07.004_bib28","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1021\/bi00678a003","article-title":"Amino acid sequence of dogfish trypsin","volume":"14","author":"Titani","year":"1975","journal-title":"Biochemistry"},{"key":"10.1016\/j.cbpb.2007.07.004_bib29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.fishres.2006.01.015","article-title":"Morphometric and meristic variation between stocks of bluefish (Pomatomus saltatrix) in the Black, Marmara, Aegean and northeastern Mediterranean seas","volume":"79","author":"Turan","year":"2006","journal-title":"Fish. Res."},{"key":"10.1016\/j.cbpb.2007.07.004_bib30","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0076-6879(70)19006-9","article-title":"Trypsinogens and trypsins of various species","volume":"19","author":"Walsh","year":"1970","journal-title":"Methods Enzymol."}],"container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495907002242?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495907002242?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T22:28:19Z","timestamp":1761085699000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096495907002242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S1096495907002242"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpb.2007.07.004","relation":{},"ISSN":["1096-4959"],"issn-type":[{"value":"1096-4959","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Trypsin from the pyloric caeca of bluefish (Pomatomus saltatrix)","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpb.2007.07.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}