{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:43:06Z","timestamp":1791171786936,"version":"4.1.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"published-print":{"date-parts":[[2012,1]]},"DOI":"10.1016\/j.cbpb.2011.09.003","type":"journal-article","created":{"date-parts":[[2011,9,17]],"date-time":"2011-09-17T06:26:36Z","timestamp":1316240796000},"page":"25-31","source":"Crossref","is-referenced-by-count":29,"title":["Cloning and localization of MCdef, a defensin from Manila clams (Ruditapes philippinarum)"],"prefix":"10.1016","volume":"161","author":[{"given":"Mausumi","family":"Adhya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Do","family":"Jeung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Sil","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Sik","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Sun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moonjae","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpb.2011.09.003_bb0005","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1111\/j.1365-2761.2010.01195.x","article-title":"Expression and localization of MCsialec, a sialic acid-specific lectin in the marine bivalve Manila clam. Ruditapes philippinarum","volume":"33","author":"Adhya","year":"2010","journal-title":"J. Fish Dis."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0010","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1128\/IAI.61.7.2978-2984.1993","article-title":"Mechanisms of action on Escherichia coli of cecropin P1 and PR-39, two antibacterial peptides from pig intestine","volume":"61","author":"Boman","year":"1993","journal-title":"Infect. Immun."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0015","doi-asserted-by":"crossref","first-page":"21808","DOI":"10.1074\/jbc.271.36.21808","article-title":"Innate immunity: isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis","volume":"271","author":"Charlet","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0020","first-page":"2434","article-title":"RGD-Tachyplesin inhibits tumor growth","volume":"61","author":"Chen","year":"2001","journal-title":"Cancer Res."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0025","author":"DeLano"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0030","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.tig.2003.10.010","article-title":"Arthropod and mollusk defensins: evolution by exon-shuffling","volume":"19","author":"Froy","year":"2003","journal-title":"Trends Genet."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0035","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1074\/jbc.M510850200","article-title":"Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure. antimicrobial activities and gene expression","volume":"281","author":"Gueguen","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0040","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0145-305X(02)00105-2","article-title":"A novel antimicrobial peptide from the sea hare Dolabella auricularia","volume":"27","author":"Iijima","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0045","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/CMR.00056-05","article-title":"Peptide antimicrobial agents","volume":"19","author":"Jenssen","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0050","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.dci.2006.03.005","article-title":"Analysis of EST and lectin expressions in hemocytes of Manila clams (Ruditapes philippinarum) (Bivalvia: Mollusca) infected with Perkinsus olseni","volume":"30","author":"Kang","year":"2006","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0065","doi-asserted-by":"crossref","first-page":"26854","DOI":"10.1074\/jbc.M601251200","article-title":"Lectin from the Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni","volume":"281","author":"Kim","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0055","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.fsi.2008.07.015","article-title":"Characterization, tissue expression, and immunohistochemical localization of MCL3, a C-type lectin produced by Perkinsus olseni-infected Manila clams (Ruditapes philippinarum)","volume":"25","author":"Kim","year":"2008","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0060","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.dci.2008.03.002","article-title":"Noble tandem-repeat galectin of Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni","volume":"32","author":"Kim","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0070","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1046\/j.1432-1327.1999.00654.x","article-title":"Myticin, a novel cysteine rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis","volume":"265","author":"Mitta","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0075","doi-asserted-by":"crossref","first-page":"4233","DOI":"10.1242\/jcs.112.23.4233","article-title":"Mussel defensins are synthesized and processed in granulocytes then released into the plasma after bacterial challenge","volume":"112","author":"Mitta","year":"1999","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0085","doi-asserted-by":"crossref","first-page":"249","DOI":"10.3354\/dao059249","article-title":"Effect of temperature on defense parameters in Manila clam Ruditapes philippinarum challenged with Vibrio tapetis","volume":"59","author":"Paillard","year":"2004","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0090","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1006\/bbrc.1998.8159","article-title":"Mechanism of action of the antimicrobial peptide buforin II: buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions","volume":"244","author":"Park","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0095","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.aquaculture.2005.12.011","article-title":"Report on the occurrence of brown ring disease (BRD) in Manila clam, Ruditapes philippinarum, on the west coast of Korea","volume":"255","author":"Park","year":"2006","journal-title":"Aquaculture"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0080","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1093\/oxfordjournals.jbchem.a124818","article-title":"A novel big defensin identified in horseshoe crab hemocytes: isolation, amino acid sequence and antibacterial activity","volume":"117","author":"Saito","year":"1995","journal-title":"J Biochem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0100","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: an automated protein homology-modeling server","volume":"31","author":"Schwede","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0105","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1177\/002215540205000802","article-title":"DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH","volume":"50","author":"Shi","year":"2002","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0110","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1128\/AAC.48.10.3645-3654.2004","article-title":"Antimicrobial peptides from marine invertebrates","volume":"48","author":"Tincu","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0120","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1016\/j.molimm.2010.01.025","article-title":"Defensin of the zebra mussel (Dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions","volume":"47","author":"Xu","year":"2010","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0115","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.cbpb.2010.05.010","article-title":"cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii","volume":"157","author":"Xu","year":"2010","journal-title":"Comp. Biochem. Physiol. Part B"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0125","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1126\/science.1076185","article-title":"Contribution of human \u03b1-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor","volume":"298","author":"Zang","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0135","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.molimm.2006.02.025","article-title":"Molecular cloning, expression of a big defensin gene from bay scallop Argopecten irradians and the antimicrobial activity of its recombinant protein","volume":"44","author":"Zhao","year":"2007","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.cbpb.2011.09.003_bb0130","doi-asserted-by":"crossref","first-page":"e13480","DOI":"10.1371\/journal.pone.0013480","article-title":"Molecular characterization of a novel big defensin from clam Venerupis philippinarum","volume":"5","author":"Zhao","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.cbpb.2011.09.003_bb0140","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.fsi.2009.11.005","article-title":"Defensin from disk abalone Haliotis discus discus: Molecular cloning, sequence characterization and immune response against bacterial infection","volume":"28","author":"Zoysa","year":"2010","journal-title":"Fish Shellfish Immunol."}],"container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495911001783?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495911001783?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T07:47:19Z","timestamp":1638690439000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096495911001783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["S1096495911001783"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpb.2011.09.003","relation":{},"ISSN":["1096-4959"],"issn-type":[{"value":"1096-4959","type":"print"}],"subject":[],"published":{"date-parts":[[2012,1]]}}}